FastQCFastQC Report
Fri 17 Jun 2016
SRR1526396_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526396_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65157
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG34325.267277498964041No Hit
AGTCGTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC9731.493316144082754No Hit
AGTCGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2810.43126601900026096No Hit
AGTCGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2340.35913255674754824No Hit
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT2310.35452829319950274No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1940.2977423761069417No Hit
AGTCGTGGGGCTTGGAAAATTGTTTTTCAACTTTTTACTCGGAGGTAGTGTCTGCCTTCGTCAC1710.2624430222385929No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1450.2225394048221987No Hit
AGTCGTGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGC1320.20258759611400157No Hit
AGTCGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.1841705419218196No Hit
AGTCGTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA1000.15347545160151635No Hit
AGTCGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC980.150405942569486No Hit
AGTCGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG920.14119741547339504No Hit
AGTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA890.13659315192534954No Hit
AGTCGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC890.13659315192534954No Hit
AGTCGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT870.13352364289331922No Hit
AGTCGTGGGTATGTGGTCAGCACAGGTAATTTGTAATGAATGAAGCAGTAGGCAGTTTCTAATG770.11817609773316759No Hit
AGTCGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT760.11664134321715242No Hit
AGTCGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG750.11510658870113726No Hit
AGTCGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.10282855257301593No Hit
AGTCGTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.10282855257301593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTGTC201.9613355E-458.20695533
CCCAAGC150.004601970358.2069533
ATGCGTT150.004601970358.2069533
TAATGAA150.004601970358.2069534
ATATGCT351.5645128E-858.20694734
CCTTCGT150.004630204358.11719555
CGGCGTG150.004630204358.11719554
CGAACTG150.004630204358.11719554
AAATCAA201.9763768E-458.11719557
ATAATTT150.004630204358.11719552
GAACTGA150.004630204358.11719555
TCGGGTC150.004630204358.11719539
GCTGCAC201.9763768E-458.11719556
TATATAA201.9763768E-458.11719552
AAGCCCC150.004630204358.11719556
TCTAATG150.004630204358.11719558
GTGTCCG201.9763768E-458.11719535
ATAAGCG150.004630204358.11719556
TTTGCTG201.9763768E-458.11719553
ATAAATC201.9763768E-458.11719555
Produced by FastQC (version 0.11.5)