SRR1526395_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1526395_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	59152
Sequences flagged as poor quality	0
Sequence length	56
%GC	51

Per base sequence quality

Per base quality graph

Per tile sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
AGTCGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA	3336	5.639707871246957	No Hit
AGTCGTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG	993	1.678725994049229	No Hit
AGTCGTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	355	0.600148769272383	No Hit
AGTCGTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA	346	0.584933730051393	No Hit
AGTCGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG	269	0.45476061671625645	No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	182	0.3076819042466865	No Hit
AGTCGTGGGGCTTGGAAAATTGTTTTTCAACTTTTTACTCGGAGGTAGTGTCTGCC	177	0.29922910467946984	No Hit
AGTCGTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	174	0.2941574249391398	No Hit
AGTCGTGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT	134	0.22653502840140655	No Hit
AGTCGTGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGC	129	0.21808222883418987	No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC	107	0.18088991073843655	No Hit
AGTCGTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC	107	0.18088991073843655	No Hit
AGTCGTGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	104	0.1758182309981066	No Hit
AGTCGTGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC	102	0.1724371111712199	No Hit
AGTCGTGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	99	0.16736543143088992	No Hit
AGTCGTGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG	94	0.15891263186367324	No Hit
AGTCGTGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA	94	0.15891263186367324	No Hit
AGTCGTGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92	0.15553151203678658	No Hit
AGTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	85	0.14369759264268325	No Hit
AGTCGTGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	84	0.14200703272923992	No Hit
AGTCGTGGGTATGTGGTCAGCACAGGTAATTTGTAATGAATGAAGCAGTAGGCAGT	82	0.13862591290235327	No Hit
AGTCGTGGGGGTGTTCGTCATGTCGGCGTCTGTGGTGTCCGTCATCTCCCGGTTCC	79	0.13355423316202328	No Hit
AGTCGTGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA	76	0.12848255342169326	No Hit
AGTCGTGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC	75	0.12679199350824993	No Hit
AGTCGTGGGGTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT	73	0.12341087368136328	No Hit
AGTCGTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA	73	0.12341087368136328	No Hit
AGTCGTGGGGGGGTTGGCGCTGCGGTGGCGTTTCCTACTGTGGTAGCCTCCGCTTC	71	0.1200297538544766	No Hit
AGTCGTGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC	68	0.1149580741141466	No Hit
AGTCGTGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGG	66	0.11157695428725994	No Hit
AGTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	65	0.1098863943738166	No Hit
AGTCGTGGGGAAGTGGCTTTAATGACCCTGAAGGAATTTCTTCTGGTGATAGCTTT	64	0.10819583446037327	No Hit
AGTCGTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	64	0.10819583446037327	No Hit
AGTCGTGGGGGTAGACAATGAGGCCATCTATGATATCTGTCGTAGAAACCTCGACA	62	0.1048147146334866	No Hit
AGTCGTGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGT	61	0.10312415472004327	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTCTAT	15	0.007913627	50.746334	37
CCATACT	15	0.007913627	50.746334	37
CCCATGC	15	0.007967814	50.658916	36
GCCATAC	15	0.007967814	50.658916	36
TACTCGG	15	0.007967814	50.658916	36
TTACTCG	15	0.007995011	50.61532	35
GGCCCAT	15	0.007995011	50.61532	34
TCTTGGA	15	0.007995011	50.61532	34
ATATGCT	45	1.1641532E-10	50.61532	34
CAGTCTC	15	0.008077017	50.48498	31
GATAAGG	15	0.008077017	50.48498	33
TTGTGTC	15	0.008077017	50.48498	31
CTTTTTA	15	0.008077017	50.48498	31
CATATGC	50	5.456968E-12	50.484978	33
CGGAGGT	15	0.008187329	50.312233	40
GTCGAAG	15	0.008187329	50.312233	40
GCTTGTC	45	1.2369128E-10	50.312233	38
ATGTCGA	15	0.008187329	50.312233	38
CATACTC	15	0.008187329	50.312233	38
ACTCGCA	15	0.008270795	50.183445	41

Summary

Basic Statistics

Per base sequence quality

Per tile sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content