FastQCFastQC Report
Fri 17 Jun 2016
SRR1526394_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526394_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566447
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC27410.4838934622303587No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC15190.26816277604082994No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC13170.2325018933810224No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG12540.22137993492771607No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC11570.20425564969008572No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC11230.1982533229057617No Hit
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC10810.19083868393689082No Hit
AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT8620.15217663788492128No Hit
AGGGTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8590.1516470208157162No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC8050.1421139135700251No Hit
AGGGTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC7200.127108096609215No Hit
AGGGTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC6620.11686883327125044No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCA6620.11686883327125044No Hit
AGGGTCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG6560.11580959913284031No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT6550.11563306010977197No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCCA6530.11527998206363527No Hit
AGGGTCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC6200.10945419430237957No Hit
AGGGTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA6000.10592341384101248No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCTGAAGGTCC5980.10557033579487578No Hit
AGGGTCGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCTCAGGAATCTCTTC5710.1008037821720302No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGTTA258.520272E-658.1638152
TTACGAA150.00463396358.1638153
TATCGGG150.00463396358.1638152
CGTATAG150.00468299958.0093832
CCGTATA150.00468299958.0093831
CCGTACG150.00469120957.98372328
CGATAGT150.004707661557.93247221
AATTGCG150.00473076757.86087812
GTCGGTA303.821151E-757.8608784
AGGGTCG573300.056.8970341
TCGGGAT38950.056.6724625
GTCGGGG336300.056.647914
GGGTCGG573400.056.5944752
GGTCGGG562150.056.5691343
TCGGGGG153700.056.562115
CGGGATA4700.056.014256
TCGGGGA87200.055.638015
GTCGGGA164300.055.5541884
TCGGGGT42250.054.7795265
TCGGGAG61350.054.7485545
Produced by FastQC (version 0.11.5)