FastQCFastQC Report
Fri 17 Jun 2016
SRR1526393_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526393_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences545289
Sequences flagged as poor quality0
Sequence length64
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC21990.4032723931713275No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC12760.2340043536546675No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG11650.21364817555461416No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC11080.2031950030167489No Hit
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC9790.17953782306263283No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC9410.17256904137072268No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC8870.16266603580853456No Hit
AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT7180.13167329617872356No Hit
AGGGTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7160.13130651819493883No Hit
AGGGTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC6660.1221370686003202No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC6430.11791912178679563No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT6160.11296761900570157No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCA5730.10508189235432955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAA258.565556E-658.11130547
TACGTAA150.004657265758.08986341
TCGGGGG143350.056.9133225
AGGGTCG549250.056.897311
GTCGGGG314850.056.6679344
GGGTCGG548050.056.6054422
GGTCGGG537050.056.5979353
TCGGGAT39850.056.5274665
GTCGGGA159300.055.9102974
TCGGGGA82250.055.7931945
TCGGGGT39900.054.7194985
CGGGATT14000.054.2526476
CGGGATA4900.054.223186
TCGGGGC53700.054.2100075
GTCGGGT24450.054.097834
TCGGGAC28800.054.0492255
TCGGGAG60450.054.0330545
CGGGAGT16500.053.908846
TCGGGCA15800.053.3726735
CGGGATC11750.053.3353426
Produced by FastQC (version 0.11.5)