##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526393_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 545289 Sequences flagged as poor quality 0 Sequence length 64 %GC 57 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.66057265046609 38.0 38.0 39.0 35.0 40.0 2 37.277130108988075 38.0 38.0 39.0 33.0 40.0 3 37.36951047976394 38.0 38.0 39.0 33.0 40.0 4 37.2462950838913 38.0 38.0 39.0 33.0 40.0 5 35.544271019587775 37.0 35.0 39.0 29.0 40.0 6 37.966731402980805 39.0 38.0 40.0 35.0 40.0 7 37.93146203206006 39.0 38.0 40.0 35.0 40.0 8 37.73403644672825 39.0 38.0 40.0 35.0 40.0 9 37.94378944009507 39.0 38.0 40.0 35.0 40.0 10 37.275912406081915 39.0 37.0 39.0 33.0 40.0 11 37.384412669245116 39.0 38.0 39.0 33.0 40.0 12 37.177184942296655 39.0 36.0 39.0 33.0 40.0 13 37.118716863901525 39.0 36.0 39.0 32.0 40.0 14 37.1259919052099 39.0 36.0 39.0 33.0 40.0 15 36.90686223268762 39.0 36.0 39.0 33.0 40.0 16 36.66293470068166 38.0 36.0 39.0 32.0 40.0 17 36.24700479928992 38.0 35.0 39.0 31.0 40.0 18 36.497913950217225 38.0 35.0 39.0 31.0 40.0 19 36.404161829782005 38.0 35.0 39.0 31.0 40.0 20 36.328115916513994 38.0 35.0 39.0 31.0 40.0 21 36.26324572841191 38.0 35.0 39.0 31.0 40.0 22 36.12482555122146 38.0 35.0 39.0 30.0 40.0 23 35.98727280396267 38.0 35.0 39.0 30.0 40.0 24 35.86855594006114 38.0 35.0 39.0 30.0 40.0 25 35.72052617969554 38.0 35.0 39.0 29.0 40.0 26 36.00278751267676 38.0 35.0 39.0 30.0 40.0 27 35.44427267008871 38.0 35.0 39.0 28.0 40.0 28 35.29573675610548 38.0 35.0 39.0 28.0 40.0 29 35.17501361663265 38.0 35.0 39.0 27.0 40.0 30 35.09686789940747 38.0 35.0 39.0 27.0 40.0 31 34.70274661693157 38.0 34.0 39.0 26.0 40.0 32 34.78473983520665 38.0 34.0 39.0 27.0 40.0 33 34.66782568509543 38.0 33.0 39.0 26.0 40.0 34 34.02718375026821 38.0 33.0 39.0 23.0 40.0 35 34.0597444657787 38.0 33.0 39.0 24.0 40.0 36 33.55293431556478 38.0 33.0 39.0 22.0 40.0 37 33.30264501943007 37.0 32.0 39.0 21.0 40.0 38 33.23376594796521 37.0 32.0 39.0 22.0 40.0 39 32.77339905994803 37.0 32.0 39.0 17.0 40.0 40 32.784794851904216 36.0 32.0 39.0 18.0 40.0 41 32.3010110235123 36.0 31.0 39.0 14.0 40.0 42 31.97227708609563 36.0 31.0 39.0 11.0 40.0 43 31.888912118161194 36.0 30.0 39.0 11.0 40.0 44 31.744728024955574 36.0 30.0 39.0 10.0 40.0 45 31.438426229027176 36.0 30.0 39.0 4.0 40.0 46 30.958471562785974 36.0 30.0 38.0 4.0 40.0 47 30.727109844504472 35.0 29.0 38.0 4.0 40.0 48 30.4726667877034 35.0 29.0 38.0 4.0 40.0 49 30.312975321343362 35.0 29.0 38.0 4.0 40.0 50 29.889917089836764 35.0 28.0 38.0 4.0 39.0 51 29.39047000764732 35.0 27.0 38.0 4.0 39.0 52 29.24734040114508 35.0 27.0 38.0 4.0 39.0 53 28.983476651830497 34.0 26.0 38.0 4.0 39.0 54 28.762089460818025 34.0 25.0 38.0 4.0 39.0 55 28.49684846017433 34.0 25.0 38.0 4.0 39.0 56 27.94302287410896 34.0 23.0 38.0 4.0 39.0 57 27.488586786089577 33.0 22.0 38.0 4.0 39.0 58 27.136478087766303 33.0 20.0 37.0 4.0 39.0 59 26.8539380035174 33.0 19.0 37.0 4.0 39.0 60 26.60113077652401 33.0 17.0 37.0 4.0 39.0 61 26.227741619581543 33.0 4.0 37.0 4.0 39.0 62 25.829989235066176 32.0 4.0 36.0 4.0 39.0 63 25.667875200123238 32.0 4.0 36.0 4.0 39.0 64 25.52534160784465 32.0 4.0 36.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -0.01614193377766071 2 2 0.08391931419026832 2 3 -0.0701869508021673 2 4 0.4242745997618229 2 5 -0.24357132777542034 2 6 0.2785852984591415 2 7 0.06739109861235448 2 8 0.4238184283889481 2 9 0.03143487185659666 2 10 0.034605549192811225 2 11 -0.23018458186152202 2 12 -0.3292795179467589 2 13 -0.1559014228444795 2 14 -0.24515719554270277 2 15 0.15167923168662867 2 16 0.0013044899289127443 2 17 0.490319591056263 2 18 0.29588905962897627 2 19 0.010009882661812242 2 20 -0.04582196917898074 2 21 -0.29817031465167076 2 22 -0.48851051980172144 2 23 -0.45252782726822716 2 24 -0.4543951940181685 2 25 -0.5510055345389375 2 26 -0.16426831252781682 2 27 0.23057117328077226 2 28 0.24246702280682797 2 29 -0.02404760883045043 2 30 -0.05377941455707713 2 31 0.3106019475791584 2 32 0.0420109964118609 2 33 -0.786508316258896 2 34 -0.016089562160985338 2 35 -0.4234390002169448 2 36 -0.932241293865772 2 37 -0.9364501139788928 2 38 -0.9594778637745449 2 39 -4.562678932045856 2 40 -1.5070263654445206 2 41 -1.8679151107539802 2 42 -1.8068336490468226 2 43 -2.2783256092272026 2 44 -1.9231423819311857 2 45 -2.077289184281714 2 46 -1.6047934799840782 2 47 -1.4482772569762652 2 48 -1.5288012744671207 2 49 -1.4014228321274302 2 50 -1.504402250246084 2 51 -1.242929818224173 2 52 -1.3718267453851674 2 53 -1.3866763544404463 2 54 -1.1375275777112677 2 55 -1.9386628442653553 2 56 -1.4583400713255799 2 57 -1.4766411356542655 2 58 -1.1745157079996353 2 59 -1.2759689109783494 2 60 -1.1370429785509657 2 61 -1.917667884525649 2 62 -1.345905345106285 2 63 -1.5092035126888597 2 64 -2.0461630055334226 3 1 -0.025343543103183208 3 2 0.4782117157873529 3 3 -0.07887608479533981 3 4 0.49200930581515223 3 5 -0.2942298968219035 3 6 0.30950974276760235 3 7 0.2793378929644206 3 8 0.47449994409210916 3 9 0.11113243492165736 3 10 0.32220364615008634 3 11 -0.2633004318821719 3 12 -0.11475122471907895 3 13 -0.13623231363369115 3 14 -0.1994321576807252 3 15 0.23689164123855733 3 16 0.014127846565855862 3 17 0.03405209423055311 3 18 -0.13605115668151058 3 19 0.0493863455111736 3 20 0.09663087544155502 3 21 0.21272934726759019 3 22 0.27660750250242927 3 23 0.0365495641558482 3 24 -0.35881472009322124 3 25 -0.19118666014893648 3 26 0.04309679937538391 3 27 0.4681952764489381 3 28 0.40902150581865726 3 29 0.4422552258465373 3 30 -0.03632083327237012 3 31 0.6558305727684655 3 32 0.5837280182417786 3 33 0.44279762386562993 3 34 0.9841934466046354 3 35 0.8994051613887066 3 36 -3.972849227289874 3 37 -3.695066583559271 3 38 -3.871997559654858 3 39 -19.414607980983426 3 40 -6.226407876558351 3 41 -4.7611787372436005 3 42 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58 1.130985920256876 118 59 0.9877890737410908 118 60 0.9104073909380119 118 61 1.123120723941284 118 62 1.1589011428696665 118 63 1.2754321649163494 118 64 1.1307220145066559 119 1 -0.3118648080977309 119 2 0.4416213849985482 119 3 0.11340308641512564 119 4 0.4344702291157674 119 5 0.5727203031053918 119 6 0.3591426035455072 119 7 0.14598638442206635 119 8 0.26705537847960414 119 9 0.15332436512885295 119 10 0.25504261379821 119 11 0.1290405350389463 119 12 0.19580493667677246 119 13 0.10779167764221143 119 14 0.012287217092087133 119 15 0.14725514541885332 119 16 0.001510616467705006 119 17 0.3631433337798029 119 18 -0.15376835042923886 119 19 -0.025611836967925683 119 20 0.1952277493056087 119 21 0.36647707536646124 119 22 0.30455370388374803 119 23 0.12043905888503303 119 24 0.1908763013535264 119 25 0.0022266441040628138 119 26 0.19162824248697774 119 27 0.912124756637958 119 28 0.6915442248299257 119 29 0.5039455202849226 119 30 0.28358102059895174 119 31 0.5123082172613778 119 32 0.25544739301459174 119 33 0.6349822839892241 119 34 0.6088899206140894 119 35 0.34718415084708454 119 36 0.6489973739460382 119 37 0.6734248740924897 119 38 0.552204548669387 119 39 0.6666024879369701 119 40 0.7842609711333992 119 41 0.3540411827854797 119 42 0.6071715041166428 119 43 0.4655316290588942 119 44 0.504719055228847 119 45 0.6043712795029954 119 46 0.1757542822069702 119 47 0.2629470964925744 119 48 0.22175507632971048 119 49 0.24796355970385164 119 50 0.35072896456449243 119 51 0.6219084297002482 119 52 0.5006954418464389 119 53 0.38882894186645345 119 54 0.483233945334824 119 55 0.350631935770906 119 56 0.10970649800355403 119 57 0.2342320392670061 119 58 0.20231032501878232 119 59 0.190865710645852 119 60 0.5536030754618224 119 61 0.5104607537031889 119 62 0.6825172142982368 119 63 0.5983525518211117 119 64 0.5121803478399904 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 424.0 5 52.0 6 99.0 7 172.0 8 435.0 9 470.0 10 768.0 11 869.0 12 1203.0 13 1666.0 14 2090.0 15 2707.0 16 3576.0 17 3811.0 18 4208.0 19 4828.0 20 5370.0 21 6493.0 22 7618.0 23 7626.0 24 8800.0 25 8975.0 26 9834.0 27 10810.0 28 12586.0 29 14262.0 30 16906.0 31 19918.0 32 24192.0 33 30296.0 34 37976.0 35 48989.0 36 62239.0 37 75409.0 38 69987.0 39 39595.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 100.0 0.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 99.20757180635484 0.5147757323679684 0.16101642074067551 0.11663604053652578 8 99.13685381011885 0.4640511722384001 0.24312684982834326 0.1559681678144089 9 97.68269669844798 1.3244352994467155 0.3790650462415343 0.6138029558637713 10 59.312217924806845 28.2488735331173 4.855040171358674 7.5838683707171795 11 39.99292118491296 24.386517974872042 17.8884958251496 17.732065015065405 12 33.95190440298631 16.136764174593658 24.73202283559727 25.179308586822767 13 26.072412977338622 15.998122096723023 28.83718541910803 29.09227950683032 14 24.60511765320775 17.533271347854075 31.450111775590557 26.41149922334762 15 28.854850313468116 18.35458603121467 27.716784859563347 25.073778795753864 16 33.573131809677534 18.62045562933343 23.75435635936755 24.052056201621483 17 32.225290767066845 19.64674601629838 23.115201416100803 25.01276180053398 18 32.816359765188736 20.285573338174803 21.921953312830446 24.97611358380602 19 30.90929763849995 22.30596986185307 21.16895811212036 25.615774387526614 20 30.386756546063193 23.34021185857752 21.884532489567572 24.38849910579172 21 31.197034966779086 20.95677704850089 24.797309316710955 23.048878668009074 22 29.848025542418792 18.885948552052216 26.14558518510368 25.120440720425314 23 31.78204585091575 19.339652917993945 24.300875315658303 24.577425915432 24 31.780918390926864 20.318454749861534 22.341533732655 25.559093126556604 25 28.73797523398181 19.566035070208304 23.038928775221503 28.65706092058838 26 26.673661465202407 19.075989559162604 25.261800512503505 28.98854846313148 27 25.726921783675426 18.718523023553644 24.883554709534458 30.671000483236472 28 27.646002160001764 22.224324264754504 22.590202114450705 27.53947146079303 29 29.965040999709895 20.989170788670638 22.88235488526324 26.163433326356223 30 29.37660056802942 19.348272580639236 25.452016945353805 25.82310990597754 31 32.27689220331009 18.848340727073587 24.380598139497277 24.49416893011905 32 34.391041711434234 18.72707671002922 23.86115901623387 23.02072256230268 33 33.17008717895944 19.05238823304568 25.551300333720466 22.226224254274417 34 28.125339345695487 20.885473703584413 29.956471725026457 21.032715225693646 35 27.15319558028518 25.78761325473285 26.816809033541233 20.242382131440745 36 28.20333570038302 25.95359601452608 23.718573736050658 22.124494549040243 37 29.83131191011153 25.252792913149648 22.998036542032168 21.91785863470665 38 30.30466388355838 25.111634042725434 23.15695737207852 21.426744701637666 39 30.666019478398248 21.817503981868885 22.98041271171269 24.536063828020175 40 28.318220884073682 21.49636252210736 23.580941934700235 26.604474659118726 41 25.231888060388474 22.29623492589524 24.802908957010033 27.66896805670625 42 24.630924058167587 19.98916406671839 25.963117284235004 29.416794590879018 43 24.877406942609312 18.08443395270239 28.062891482339413 28.975267622348884 44 26.147535095168184 18.151491763388293 26.561806362720375 29.139166778723148 45 27.33140834403625 19.457440476738892 25.75738869735225 27.453762481872605 46 25.629627172248306 21.79168901347812 25.719567328869502 26.859116485404076 47 25.452179714482654 20.328827816009763 27.310475235627372 26.908517233880215 48 27.42062402444351 19.695230162225716 28.869100820250665 24.015044993080114 49 28.46547291008119 18.88543807426112 26.848095549677907 25.800993465979783 50 24.56576048651062 20.208604713809684 27.44021019148237 27.785424608197317 51 24.25660427452542 21.114319217664498 27.756021652875496 26.873054854934587 52 24.981236457516943 20.350744548430225 27.91461896639159 26.75340002766124 53 24.563866088720836 19.77349442036337 29.115927326385687 26.546712164530113 54 24.783202080374764 19.11086110547574 28.240349680013278 27.86558713413622 55 25.320719770063207 19.707339750803413 25.675107275551508 29.29683320358188 56 25.543747001734378 21.38399940957231 24.1132514114912 28.95900217720211 57 27.1286851053388 23.161437087302332 23.702383522166443 26.007494285192422 58 25.51472461264135 26.56300184957526 22.891908754287062 25.030364783496324 59 27.42460715973535 25.563415642722116 23.024353142722116 23.987624054820415 60 30.34205238819862 22.78299378019413 22.760094257390637 24.114859574216617 61 29.229477646364188 21.216857021606362 23.445209144417586 26.10845618761186 62 25.75080049090624 20.663092524752933 25.45293482573751 28.133172158603315 63 24.908554027867492 20.601642520992893 24.042262618805943 30.44754083233367 64 25.893881320711152 20.73368737012237 23.085107365504502 30.28732394366197 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 1.0 6 2.0 7 10.5 8 19.0 9 15.5 10 125.5 11 239.0 12 342.5 13 509.0 14 572.0 15 605.0 16 887.0 17 1136.0 18 1309.5 19 1483.0 20 1504.5 21 1752.5 22 1979.0 23 2447.5 24 3208.0 25 3500.0 26 3833.5 27 4420.0 28 4673.0 29 4820.0 30 4637.5 31 4308.0 32 4489.5 33 4671.0 34 4685.5 35 4642.0 36 4584.0 37 4613.0 38 4960.5 39 5279.0 40 5837.0 41 6786.0 42 7177.0 43 7390.5 44 7604.0 45 8051.0 46 8810.5 47 9123.0 48 9784.5 49 11427.0 50 12408.0 51 13749.0 52 15252.5 53 15415.0 54 17466.5 55 22352.5 56 25187.0 57 27229.5 58 29272.0 59 31672.5 60 39071.5 61 44070.0 62 46155.5 63 48028.5 64 47816.0 65 42486.5 66 33930.0 67 30703.0 68 27297.0 69 23891.0 70 20545.5 71 14949.0 72 12698.0 73 10882.5 74 7988.0 75 6909.0 76 5599.0 77 3468.5 78 2648.0 79 2164.5 80 1359.5 81 1038.0 82 799.0 83 560.0 84 441.5 85 241.5 86 160.0 87 129.0 88 68.5 89 39.0 90 27.5 91 14.0 92 12.0 93 8.0 94 4.0 95 4.0 96 2.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 5.501669756771179E-4 8 0.05611703151906604 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.07647320961911941 16 0.01998940011626862 17 0.127455349365199 18 0.0 19 0.0 20 0.020906345075730484 21 0.0 22 0.0 23 0.0 24 0.005685058748663553 25 0.04914824982715588 26 0.022373457010869468 27 0.19109132955185232 28 0.15496369814905492 29 0.11938623372193462 30 0.1744029312896464 31 0.20814650579784297 32 0.14854508343282186 33 0.20557905991134975 34 0.36017598007661994 35 0.23327079768709802 36 0.31451212109541915 37 0.34110352491981316 38 0.32496529363328436 39 0.6341591339638247 40 0.3517400864495708 41 0.3922690536577851 42 0.4859808285147876 43 0.48414693859586383 44 0.4416006924768334 45 0.4771781569039537 46 0.4962506120607605 47 0.49423333314994433 48 0.4859808285147876 49 0.5032193937526707 50 0.6064673961880764 51 0.5342121333824816 52 0.5527344215636112 53 0.5756580455501579 54 0.5646547060366155 55 0.5917962768366867 56 0.6068341741718611 57 0.6007823374394128 58 0.6497471982746763 59 0.658916647869295 60 0.6955944462477696 61 0.6086680640907849 62 0.6297577981584077 63 0.6306747431178695 64 0.7177845142667466 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 545289.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.01631934245114 #Duplication Level Percentage of deduplicated Percentage of total 1 87.79684557862312 50.936498303492684 2 4.792586856155774 5.560964990463346 3 2.04523506146811 3.559710313695347 4 1.2528073704777731 2.907330899208599 5 0.8502528137395321 2.466426938186516 6 0.6184560595620545 2.1528326550495684 7 0.4340988867176913 1.762937374661127 8 0.33582106951135826 1.5586481928555556 9 0.2723135585265237 1.421876733547865 >10 1.441103092113158 14.94066713749292 >50 0.09526708037564477 3.725985109506745 >100 0.060959578855693884 6.778031603699701 >500 0.0031188621740120344 1.2825703777222333 >1k 0.0011341316996407401 0.9455193704177757 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC 2199 0.4032723931713275 No Hit AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC 1276 0.2340043536546675 No Hit AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG 1165 0.21364817555461416 No Hit AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC 1108 0.2031950030167489 No Hit AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC 979 0.17953782306263283 No Hit AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC 941 0.17256904137072268 No Hit AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 887 0.16266603580853456 No Hit AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT 718 0.13167329617872356 No Hit AGGGTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 716 0.13130651819493883 No Hit AGGGTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC 666 0.1221370686003202 No Hit AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC 643 0.11791912178679563 No Hit AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 616 0.11296761900570157 No Hit AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCCA 573 0.10508189235432955 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 7.335559675694907E-4 0.0 0.0 0.0 0.0 8 9.169449594618633E-4 0.0 0.0 0.0 0.0 9 9.169449594618633E-4 0.0 0.0 0.0 0.0 10 0.002567445886493217 0.0 0.0 0.0 0.0 11 0.004034557821632198 0.0 0.0 0.0 0.0 12 0.004768113789201689 0.0 0.0 0.0 0.0 13 0.005318280764878808 0.0 0.0 0.0 0.0 14 0.008619282618941515 0.0 0.0 0.0 0.0 15 0.009719616570295752 0.0 0.0 0.0 0.0 16 0.010453172537865242 0.0 0.0 0.0 0.0 17 0.011370117497327106 0.0 0.0 0.0 0.0 18 0.01228706245678897 0.0 0.0 0.0 0.0 19 0.013937563383820323 0.0 0.0 0.0 0.0 20 0.016688398262205913 0.0 0.0 0.0 0.0 21 0.01778873221356015 0.0 0.0 0.0 0.0 22 0.019622622132483874 0.0 0.0 0.0 0.0 23 0.021823290035192348 0.0 0.0 0.0 0.0 24 0.023840568946008448 0.0 0.0 0.0 0.0 25 0.025857847856824545 0.0 0.0 0.0 0.0 26 0.02732495979196353 0.0 0.0 0.0 0.0 27 0.02879207172710251 0.0 0.0 0.0 0.0 28 0.03686118737036691 0.0 0.0 0.0 0.0 29 0.03777813232982877 0.0 0.0 0.0 0.0 30 0.04254624611903046 0.0 0.0 0.0 0.0 31 0.043646580070384695 0.0 0.0 0.0 0.0 32 0.04529708099741605 0.0 0.0 0.0 0.0 33 0.0469475819244474 0.0 0.0 0.0 0.0 34 0.0489648608352635 0.0 0.0 0.0 0.0 35 0.060885145308267725 0.0 0.0 0.0 0.0 36 0.06473631413800755 0.0 0.0 0.0 0.0 37 0.06730376002450077 0.0 0.0 0.0 0.0 38 0.07133831784613297 0.0 0.0 0.0 0.0 39 0.07555626465965753 0.0 0.0 0.0 0.0 40 0.07739015457858127 0.0 0.0 0.0 0.0 41 0.07977421147318212 0.0 0.0 0.0 0.0 42 0.08142471240021347 0.0 0.0 0.0 0.0 43 0.08472571425427618 0.0 0.0 0.0 0.0 44 0.08876027207590838 0.0 0.0 0.0 0.0 45 0.09224466292186345 0.0 0.0 0.0 0.0 46 0.09554566477592616 0.0 0.0 0.0 0.0 47 0.09884666662998887 0.0 0.0 0.0 0.0 48 0.10178089050026683 0.0 0.0 0.0 0.0 49 0.10434833638676005 0.0 0.0 0.0 0.0 50 0.10728256025703801 0.0 0.0 0.0 0.0 51 0.11095034009488547 0.0 0.0 0.0 0.0 52 0.11443473094084054 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGAA 25 8.565556E-6 58.111305 47 TACGTAA 15 0.0046572657 58.089863 41 TCGGGGG 14335 0.0 56.913322 5 AGGGTCG 54925 0.0 56.89731 1 GTCGGGG 31485 0.0 56.667934 4 GGGTCGG 54805 0.0 56.605442 2 GGTCGGG 53705 0.0 56.597935 3 TCGGGAT 3985 0.0 56.527466 5 GTCGGGA 15930 0.0 55.910297 4 TCGGGGA 8225 0.0 55.793194 5 TCGGGGT 3990 0.0 54.719498 5 CGGGATT 1400 0.0 54.252647 6 CGGGATA 490 0.0 54.22318 6 TCGGGGC 5370 0.0 54.210007 5 GTCGGGT 2445 0.0 54.09783 4 TCGGGAC 2880 0.0 54.049225 5 TCGGGAG 6045 0.0 54.033054 5 CGGGAGT 1650 0.0 53.90884 6 TCGGGCA 1580 0.0 53.372673 5 CGGGATC 1175 0.0 53.335342 6 >>END_MODULE