FastQCFastQC Report
Fri 17 Jun 2016
SRR1526392_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526392_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences514320
Sequences flagged as poor quality0
Sequence length56
%GC58

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC48710.9470757505055218No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC20210.39294602582050087No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC15420.2998133457769482No Hit
AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT11450.22262404728573654No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG10030.19501477679265827No Hit
AGGGTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT9770.1899595582516721No Hit
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA9190.17868253227562605No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG9180.17848810079328045No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC8410.1635168766526676No Hit
AGGGTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT8090.15729506921760772No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT7050.13707419505366308No Hit
AGGGTCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC6890.13396329133613316No Hit
AGGGTCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA6180.12015865608959403No Hit
AGGGTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT6080.1182143412661378No Hit
AGGGTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG5710.11102037641934981No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCG5670.11024265048996734No Hit
AGGGTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG5270.10246539119614247No Hit
AGGGTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG5220.10149323378441438No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACACC150.00805193450.5812434
TCGTACC150.00830655550.1844547
ACGTAGG355.1346433E-850.1795346
GTATTCG150.00830977550.17952750
GTCTAGT150.0083258950.15493845
ACGTATG150.00845564749.95909529
ATAGTGC150.00846870549.93959828
TCGACGG150.00854079249.8326223
CTCCGTA150.00857700649.779320
TCGGTAC252.1436097E-549.769625
GTCGGTA402.7903297E-949.769624
ACAACGG150.00859350549.755112
ATTAACG150.00859350549.755114
ATCGGAT150.00859350549.755112
GATTAGT150.00859350549.75519
TCGGGAT34000.049.2572865
CGGGATA3700.049.0970576
AGGGTCG518600.049.0018651
GGGTCGG517950.048.7750932
GGTCGGG508550.048.727353
Produced by FastQC (version 0.11.5)