##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526392_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	514320
Sequences flagged as poor quality	0
Sequence length	56
%GC	58
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.79626108259449	38.0	38.0	40.0	35.0	40.0
2	37.394237050863275	38.0	38.0	40.0	34.0	40.0
3	37.509414372375176	38.0	38.0	40.0	34.0	40.0
4	37.44864286825323	38.0	38.0	40.0	34.0	40.0
5	35.29084616581117	37.0	34.0	39.0	29.0	40.0
6	38.230154378596986	39.0	38.0	40.0	36.0	40.0
7	38.05117825478302	39.0	38.0	40.0	36.0	40.0
8	38.18981179032509	39.0	38.0	40.0	36.0	40.0
9	38.19955864053507	39.0	38.0	40.0	36.0	40.0
10	37.55936187587494	39.0	38.0	40.0	33.0	40.0
11	37.815708119458705	39.0	38.0	40.0	35.0	40.0
12	37.71211696997978	39.0	38.0	40.0	34.0	40.0
13	37.700904106392905	39.0	38.0	40.0	34.0	40.0
14	37.74245216985534	39.0	38.0	40.0	34.0	40.0
15	37.728509488256336	39.0	38.0	40.0	34.0	40.0
16	37.64607831700109	39.0	38.0	40.0	34.0	40.0
17	37.46994672577384	39.0	38.0	40.0	33.0	40.0
18	37.409814901228806	39.0	37.0	40.0	33.0	40.0
19	37.31422655156323	39.0	37.0	40.0	33.0	40.0
20	37.24715352309846	39.0	37.0	40.0	33.0	40.0
21	37.074342821589674	39.0	36.0	40.0	33.0	40.0
22	36.96602115414528	38.0	36.0	40.0	33.0	40.0
23	36.8152025976046	38.0	36.0	40.0	32.0	40.0
24	36.68452325400529	38.0	36.0	40.0	32.0	40.0
25	36.546587727484834	38.0	36.0	40.0	31.0	40.0
26	36.01346632446726	38.0	35.0	39.0	30.0	40.0
27	35.78364636801991	38.0	35.0	39.0	29.0	40.0
28	35.510635402084304	38.0	35.0	39.0	29.0	40.0
29	35.33828939181832	38.0	35.0	39.0	29.0	40.0
30	35.06787797480168	38.0	34.0	39.0	28.0	40.0
31	34.349881396795766	38.0	33.0	39.0	26.0	40.0
32	34.11662583605538	38.0	33.0	39.0	25.0	40.0
33	33.881742883807746	37.0	33.0	39.0	24.0	40.0
34	34.132501166588895	38.0	33.0	39.0	25.0	40.0
35	33.92323261782548	38.0	33.0	39.0	25.0	40.0
36	33.55138046352465	38.0	33.0	39.0	23.0	40.0
37	33.03613120236429	37.0	32.0	39.0	19.0	40.0
38	33.03922071861876	37.0	32.0	39.0	20.0	40.0
39	32.88452714263494	37.0	32.0	39.0	19.0	40.0
40	32.446245528075906	36.0	31.0	39.0	15.0	40.0
41	31.84589360709286	36.0	30.0	38.0	10.0	40.0
42	31.66361798102349	36.0	30.0	38.0	9.0	40.0
43	31.473909239384042	36.0	30.0	38.0	4.0	40.0
44	31.02862809146057	36.0	29.0	38.0	4.0	40.0
45	30.748775081661222	35.0	29.0	38.0	4.0	40.0
46	30.010674288380773	35.0	28.0	38.0	4.0	40.0
47	29.69367125524965	35.0	28.0	38.0	4.0	40.0
48	29.240991989422927	35.0	27.0	38.0	4.0	39.0
49	29.13885518743195	34.0	27.0	38.0	4.0	39.0
50	28.709194664800126	34.0	26.0	38.0	4.0	39.0
51	27.893105459636026	34.0	23.0	38.0	4.0	39.0
52	27.605156322911807	33.0	23.0	38.0	4.0	39.0
53	27.26180782392285	33.0	20.0	38.0	4.0	39.0
54	26.964955669622025	33.0	17.0	38.0	4.0	39.0
55	26.492522165188987	33.0	4.0	38.0	4.0	39.0
56	16.16911067039975	20.0	4.0	24.0	4.0	27.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	-0.6707626699679707
1	2	-0.8174638666189082
1	3	-1.309150315143512
1	4	-1.430213059199744
1	5	0.921272830640838
1	6	-0.7651152214912358
1	7	-1.7225655943455962
1	8	-2.1281094144067225
1	9	-2.3224964140129956
1	10	-0.6326721086894196
1	11	-0.5637283670728053
1	12	-0.5342660320690626
1	13	-0.9494870012133916
1	14	-0.6827592157684421
1	15	-0.5886910824566058
1	16	-0.7979716856109107
1	17	-0.7207725500915743
1	18	-0.6335682782618051
1	19	-0.6656315257769521
1	20	-1.096541164549663
1	21	-0.8740593817243081
1	22	-0.9569445404538826
1	23	-1.0130636213505326
1	24	-1.033310247524696
1	25	-0.7794421487095633
1	26	-0.5010678775815194
1	27	-0.5862112181117212
1	28	-0.5225192514335788
1	29	-0.8176569898209536
1	30	-0.4754219552124752
1	31	-0.3022134245054886
1	32	-0.39477257434481317
1	33	-0.39947356039217397
1	34	-0.008957783146854581
1	35	0.21436500190083052
1	36	0.5334803968113278
1	37	0.6978185553936669
1	38	0.1948134573592739
1	39	0.6511416185287828
1	40	0.6444788644854142
1	41	0.9559044985704581
1	42	1.0555452604968423
1	43	1.1453558605509322
1	44	0.9425101261397657
1	45	1.0913893486803516
1	46	0.9278892470001985
1	47	0.7505203855392857
1	48	1.189627176679167
1	49	1.068902560467066
1	50	1.0475023751992616
1	51	1.5023081747985394
1	52	1.8035447822704107
1	53	1.7883290391588602
1	54	2.0697133969703927
1	55	2.1218525742193926
1	56	2.1375818485008615
2	1	-0.19053748142567883
2	2	0.2940239356683847
2	3	-0.28921174629459756
2	4	-0.009422951916526756
2	5	0.46152174187547956
2	6	0.16904988136611365
2	7	0.3164352200154852
2	8	0.06384522922179059
2	9	0.13373610377902168
2	10	0.20775295238765779
2	11	-0.3275636536925006
2	12	-0.3311139235820022
2	13	-0.10795547127676741
2	14	-0.20153377937712236
2	15	-0.10441621788839939
2	16	-0.3047388491558607
2	17	-0.0407367890180339
2	18	-0.1292619014604739
2	19	-0.2991247807427868
2	20	-0.3260980459007996
2	21	-0.05116521325778223
2	22	-0.13649788110466687
2	23	-0.08363420521202158
2	24	-0.1526274234620928
2	25	-0.15500866076564535
2	26	0.33652019733970207
2	27	0.0848223123205969
2	28	0.13930845808737047
2	29	0.21620980903185938
2	30	0.2634779491886121
2	31	0.4411964469670835
2	32	0.14436544215485014
2	33	0.7885774610998766
2	34	0.31559166273353867
2	35	0.7488735526921744
2	36	0.45156629420182526
2	37	0.5786164522176591
2	38	0.8933095448853905
2	39	0.5344180055981411
2	40	0.5597941638516346
2	41	0.26725155801881684
2	42	-0.024505548553189982
2	43	0.21582907515277938
2	44	0.36601772094201834
2	45	0.35466697499483857
2	46	-0.07422632145924268
2	47	0.9477595244409613
2	48	0.969107932972765
2	49	0.7949275932185458
2	50	0.7677958992583207
2	51	0.8585468888161714
2	52	1.2168286048105088
2	53	1.1572301828050726
2	54	1.244274350126929
2	55	0.7510306006330154
2	56	0.4778697074393605
3	1	-0.4180092256472463
3	2	0.33062068413256185
3	3	-0.3737796789558985
3	4	-0.15013757703955832
3	5	-0.4114121073591548
3	6	-0.04814202034111048
3	7	0.1863907574692405
3	8	0.11689905944953694
3	9	0.06738593577168928
3	10	0.2549095555848879
3	11	-0.1202527892184122
3	12	0.024140972389211868
3	13	-0.1479675202873878
3	14	-0.0701910783622921
3	15	-0.1525401360230063
3	16	-0.653544978877882
3	17	-0.23529436971246298
3	18	-0.45783078446627457
3	19	-0.3466165500805971
3	20	-0.5446136417328589
3	21	-0.38656893996585495
3	22	-0.4407905532697569
3	23	-0.14670907878225847
3	24	-0.499524378965603
3	25	-0.5939787249138959
3	26	0.010721738255426772
3	27	-0.297657408006593
3	28	-0.11304194750609042
3	29	-0.1882126869800942
3	30	0.01829755343801054
3	31	0.16533774282969205
3	32	0.248042087438769
3	33	-0.09095364571205522
3	34	0.18504657228110233
3	35	0.1880133212154078
3	36	0.49170137888430787
3	37	0.6838713481888732
3	38	0.6815481831774832
3	39	0.6640424498163142
3	40	0.3801705681553358
3	41	0.3065253929982781
3	42	0.29767396473466334
3	43	0.14890191227052085
3	44	0.6835812752304484
3	45	0.5389744114405488
3	46	0.21147947903629216
3	47	0.01553520919811291
3	48	0.044511829385214696
3	49	-0.08049666771890429
3	50	0.38208331058778455
3	51	0.10080836931713932
3	52	0.11192160280799968
3	53	0.4662151809383239
3	54	-0.02024810176187941
3	55	0.04986727714253547
3	56	0.009731703841627137
4	1	-0.30684963425049716
4	2	0.247621009235651
4	3	-0.31788333551015313
4	4	-0.21225793362631862
4	5	-0.608522967513494
4	6	-0.033683488096016845
4	7	0.13451542594963684
4	8	0.03595266723831969
4	9	0.015476382873998773
4	10	-0.24426913134083605
4	11	-0.17528076519500502
4	12	-0.23551373390581176
4	13	-0.22543616494971275
4	14	-0.22642878005454037
4	15	-0.41184407319255456
4	16	-0.4468435775124533
4	17	-0.45267284104350125
4	18	-0.5258714394634509
4	19	-0.4767091189083459
4	20	-0.39724355463945216
4	21	-0.4271982711090203
4	22	-0.2765364937587762
4	23	-0.7348162943598311
4	24	-0.4874228441368018
4	25	-0.6248806293335889
4	26	-0.439695762557335
4	27	-0.592016869362098
4	28	-0.31835310538641437
4	29	-0.42401509274296956
4	30	-0.5211040857659981
4	31	-0.06705108046821806
4	32	-0.21749270714741442
4	33	-0.702023405991298
4	34	-0.45598400452022503
4	35	-0.4092181692967145
4	36	-0.13829280348141282
4	37	0.27752242701782137
4	38	-0.133600765370673
4	39	0.3469942147838907
4	40	0.3395326816676487
4	41	0.015134636192502882
4	42	0.3021270215592615
4	43	0.40000127404181995
4	44	0.17408912048137637
4	45	0.09437026723166042
4	46	0.7650231027380201
4	47	0.4378184595446051
4	48	0.4760006304392306
4	49	0.35553929894947345
4	50	0.4524253532618445
4	51	0.8511291947367781
4	52	1.0797706180878421
4	53	1.0766347350337675
4	54	0.4636721282057792
4	55	0.5429117820447473
4	56	0.6148768880381965
5	1	-0.17559051903416645
5	2	0.10415618528954695
5	3	-0.22961331675885077
5	4	-0.24790277007329564
5	5	-0.17861464054634268
5	6	-0.35483426245534844
5	7	0.14595719273899732
5	8	0.05229060737305247
5	9	0.08812031648049867
5	10	-0.11858331982614345
5	11	-0.20337299382199348
5	12	-0.012182252552939588
5	13	-0.16590078054801438
5	14	-0.25436474783008123
5	15	-0.07371295297587466
5	16	-0.3936801633864704
5	17	-0.3519366788100129
5	18	-0.2508523409149461
5	19	-0.5929324677520142
5	20	-0.6523654381035797
5	21	-0.2003248771233288
5	22	-0.04266608609157885
5	23	-0.027996376309964432
5	24	-0.25162521930174364
5	25	-0.487475253960298
5	26	-0.08378562990923655
5	27	0.11891791777589589
5	28	0.16131562160851587
5	29	-0.05817371486950407
5	30	0.06345039718420509
5	31	0.05679432546274654
5	32	-0.06449597126297846
5	33	-0.4684655200269958
5	34	-0.16618957435146342
5	35	-0.15564153203023778
5	36	-0.14817196175629022
5	37	-2.4532922128877317
5	38	-0.1551647628704984
5	39	-0.33982431428062654
5	40	-0.4587345591129619
5	41	-0.30433199343455186
5	42	-0.22981964140344502
5	43	-0.49547757865628483
5	44	-0.22154599306817602
5	45	-0.025481805828967197
5	46	0.15600511537778772
5	47	0.22415778866468372
5	48	0.307360447093167
5	49	-0.009677035518194543
5	50	0.24906053626066438
5	51	0.6405416539353332
5	52	0.6474836537153195
5	53	0.728607094225378
5	54	0.3273953034262824
5	55	0.27794546493939265
5	56	0.7676479138038736
6	1	-0.08904625040268854
6	2	0.442145590497212
6	3	0.035286688417762946
6	4	-0.2554045387479107
6	5	0.1273833233209274
6	6	0.16950011195149983
6	7	0.37852496317366047
6	8	0.13186370971460093
6	9	0.012977528359087387
6	10	0.6051971574571695
6	11	-0.012520061273207261
6	12	0.11512349520818077
6	13	0.1888306397408357
6	14	0.07872844838064452
6	15	0.21088404804218897
6	16	-0.02895788843952829
6	17	0.1798088736204022
6	18	-0.10819363472199228
6	19	0.14032551716680075
6	20	0.24862760798549033
6	21	0.07614484834385848
6	22	-0.09993663291400168
6	23	0.05334108680669658
6	24	0.05577042889593997
6	25	-0.3369481026480301
6	26	0.33308666315583935
6	27	0.2919115505472831
6	28	0.22888330576636662
6	29	-0.010622250752035711
6	30	0.2312717351649809
6	31	0.2599891557562586
6	32	0.3398791257984328
6	33	0.12048155777743119
6	34	0.014543088323463849
6	35	0.17109306324524454
6	36	-0.15854243438066362
6	37	-0.9232980797108468
6	38	-0.02097412562997647
6	39	-0.15691316214009987
6	40	0.24120763542399004
6	41	0.4232964295979009
6	42	0.629544843610276
6	43	0.34629075085210914
6	44	0.44050774012941574
6	45	0.5641209757354027
6	46	0.7672504348608165
6	47	1.6182875144769788
6	48	1.4148656668402033
6	49	1.4619378938210943
6	50	1.0005272420394853
6	51	1.1164574822789923
6	52	1.7315431502038514
6	53	1.1002444555354067
6	54	1.2135703073087392
6	55	1.6567136046388065
6	56	0.15256759009328036
7	1	-0.08968365628906838
7	2	0.35461136736237364
7	3	-0.08453278454596358
7	4	0.03360983496872194
7	5	-0.03806842205894867
7	6	0.08728758628414823
7	7	0.23081363531602506
7	8	0.03051189588845915
7	9	0.026495666620554914
7	10	0.11284175020186638
7	11	-0.0017098285558887483
7	12	0.04501312559832371
7	13	0.042625129816130425
7	14	0.09826130259008892
7	15	-0.2056457260851161
7	16	-0.14131671800831924
7	17	-0.24022449393606848
7	18	-0.2061744697664878
7	19	-0.28016986270999666
7	20	-0.523741488523747
7	21	-0.4053318799244465
7	22	-0.24390908329046113
7	23	-0.43851808499344
7	24	-0.2882148551560846
7	25	-0.188000464044336
7	26	0.07323467821401408
7	27	0.034446629260493467
7	28	0.20594097174856785
7	29	0.377172923785956
7	30	0.28540417869680823
7	31	0.04331393330588185
7	32	0.10003484106195515
7	33	0.05336571246599675
7	34	0.4637474004384572
7	35	-0.2449447533187552
7	36	0.3349337805406236
7	37	0.610993501397985
7	38	0.3515404192023297
7	39	0.602280027456068
7	40	0.5364335081139302
7	41	0.9838157656690889
7	42	1.0237868011735856
7	43	1.1497430095790087
7	44	0.9997950433463885
7	45	0.48390194767243244
7	46	0.9620100173385708
7	47	1.1881283768999786
7	48	1.0330082061968078
7	49	1.0609111997759229
7	50	0.9942986314987579
7	51	1.1498378724227294
7	52	0.7862616649197989
7	53	1.2488626964662757
7	54	0.7867265359192785
7	55	0.7271576498133427
7	56	0.8104175216470111
8	1	-0.011724972815677859
8	2	0.618862067642489
8	3	0.17164718744282936
8	4	0.22058462488468678
8	5	0.1638223342435765
8	6	0.3972665778807851
8	7	0.5031000498818514
8	8	0.22035783426380817
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114	1	8.800692011305955E-4
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116	3	0.06417072046119188
116	4	0.13124931994726552
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116	6	-0.2326158471922497
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116	14	-0.35876659440562264
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116	16	0.0036671875710823088
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116	18	0.11692996514052822
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116	37	1.0281608833722302
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116	40	1.1470558257104955
116	41	0.8098797856976994
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116	43	1.0565294902657065
116	44	1.1709234410574076
116	45	1.2711694727081984
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116	49	0.4523275087887981
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116	54	1.0040101553756458
116	55	0.6104462764227847
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117	1	0.08678207092039969
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117	3	-0.14875947208945917
117	4	0.08841150511486262
117	5	0.2934033942249741
117	6	-0.2819451903338077
117	7	-2.654046315971378
117	8	-0.3576057102354113
117	9	-0.46184810512614405
117	10	-0.2912074843144907
117	11	-0.49492051053222497
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117	34	0.020193120688979604
117	35	0.14258569205355087
117	36	1.0964377819324156
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117	38	1.0617744136351774
117	39	1.0415067149995636
117	40	1.2730201469448232
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118	1	-0.11575688861229594
118	2	0.34867702392803324
118	3	-0.2047979360611123
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118	5	0.3667295577390348
118	6	0.018474454971013188
118	7	0.2726696959220902
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118	10	-0.23242340131328376
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118	38	-0.24044190402999277
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118	48	0.10125315569175797
118	49	0.5588909977557179
118	50	-0.04307510587497987
118	51	-4.777841429266516E-4
118	52	-0.4451145977064641
118	53	-0.4361751823216018
118	54	-0.2836648782221367
118	55	0.09956926597496008
118	56	-0.23737288239339271
119	1	-0.05490086386174653
119	2	0.325106634434718
119	3	0.0025657617691123846
119	4	0.04121632584093504
119	5	0.2640106854461237
119	6	-0.01572965509516422
119	7	0.2592576853498372
119	8	0.05852068561124213
119	9	0.10340574099512878
119	10	0.074331777247167
119	11	-0.07225157973911678
119	12	-0.13960908535502625
119	13	-0.2959355530777188
119	14	-0.19836648645656396
119	15	-0.12826235894515747
119	16	-0.4559085029981773
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119	19	-0.30736306757815157
119	20	-0.7813860118503229
119	21	-0.3209420895255306
119	22	-0.5178906586792351
119	23	-0.35652433921926985
119	24	-0.43954176523045874
119	25	-0.3983706466001138
119	26	-0.7154865861538013
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119	28	-0.264575424465086
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119	45	-0.15855917470755188
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119	47	-0.412759047035113
119	48	-0.0094317870418692
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119	51	0.02687894747343833
119	52	-0.2240028719294216
119	53	-0.3807814887940353
119	54	-0.10103123960472615
119	55	-0.008275920166372686
119	56	-0.5624737900500811
120	1	-0.0703752370450772
120	2	0.27218009588471404
120	3	-0.12613501822293927
120	4	-0.37927676296942536
120	5	0.3704367325802309
120	6	-0.2584598363962698
120	7	0.0766684463125884
120	8	-0.17868054397408883
120	9	-0.32281704815608236
120	10	-0.3661316174957321
120	11	-0.10637048147685135
120	12	-0.3456440908965206
120	13	-0.4531008152010543
120	14	-0.20882632627589004
120	15	-0.4948209838170783
120	16	-0.5502729035753262
120	17	-0.5000827413587459
120	18	-0.5833222360895078
120	19	-0.8083270792048438
120	20	-0.8339863338845745
120	21	-0.47796933696912447
120	22	-0.704712347895267
120	23	-0.5741065729659454
120	24	-0.6898042022351234
120	25	-0.6742118042505183
120	26	-0.6119672118203496
120	27	-0.7923789721140793
120	28	-0.5640547327187875
120	29	-0.8217317153893049
120	30	-1.1407556151176266
120	31	-0.8015743828453168
120	32	-0.7450252963950916
120	33	-0.745624837362179
120	34	-0.4201443521388626
120	35	-1.0060487052087908
120	36	-0.771874638703359
120	37	-0.1704497975710879
120	38	-0.6567284811413145
120	39	-0.4574063986607726
120	40	-0.3071231929170004
120	41	-0.040002962853247226
120	42	-0.24601560432469682
120	43	-0.16230595949315685
120	44	0.17185260348384546
120	45	-0.5566478805062616
120	46	-0.2569118039672702
120	47	0.2480510573123489
120	48	0.15519463231364483
120	49	-0.2473362229044902
120	50	0.3307247483372464
120	51	0.4973249858247897
120	52	0.1322882972222068
120	53	-0.05360723480520235
120	54	-0.16780525102230115
120	55	0.17000558795767162
120	56	-0.458762031617594
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	276.0
5	27.0
6	46.0
7	77.0
8	177.0
9	205.0
10	223.0
11	351.0
12	446.0
13	708.0
14	996.0
15	1359.0
16	1746.0
17	2385.0
18	3045.0
19	3482.0
20	3690.0
21	4704.0
22	4858.0
23	5386.0
24	7231.0
25	9074.0
26	9087.0
27	10364.0
28	11765.0
29	13380.0
30	15436.0
31	18011.0
32	21684.0
33	27174.0
34	35249.0
35	47629.0
36	64554.0
37	80806.0
38	76130.0
39	32559.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	100.0	0.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	100.0	0.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	99.1395879048197	0.5441041442955631	0.2194313460012139	0.09687660488351464
8	99.39726240472856	0.41122258516098925	0.09799346710219319	0.09352154300824389
9	97.85308757193964	1.1994478145901384	0.3573650645512521	0.590099548918961
10	59.74801679888008	27.833255560740394	4.85748172344066	7.56124591693887
11	39.83356665111214	23.997900139990666	17.835977601493234	18.33255560740395
12	33.46865764504589	15.883496655778503	25.058329444703688	25.589516254471924
13	25.801057707263958	15.943770415305647	28.91779436926427	29.337377508166124
14	24.368097682376728	17.19552029864676	31.967452169855342	26.46892984912117
15	28.431521231917873	18.230673510654846	27.97071861875875	25.367086638668535
16	33.12004199720019	18.403523098460102	23.937043086016487	24.53939181832322
17	31.913594649245606	19.577111525898275	23.13890185098771	25.37039197386841
18	32.60752060973713	20.155350754394153	22.08255560740395	25.15457302846477
19	30.897106859542696	21.81035153212008	21.434709908228342	25.85783170010888
20	30.15671177477057	22.692681599004512	22.453336444237053	24.697270181987868
21	31.03418105459636	20.623152900917717	24.740822834033285	23.601843210452635
22	29.81081816767771	18.60203764193498	26.314162389174058	25.272981801213252
23	31.67416855429066	19.204378639549695	24.268395827459823	24.853056978699826
24	31.417005465500846	20.2124370767445	22.59715311762979	25.773404340124866
25	28.803583550367907	19.61616487754971	22.97836915781509	28.601882414267294
26	27.001842272668025	19.006923599182162	25.200617073801794	28.790617054348015
27	26.159436650421185	18.286089095806798	24.738800741167495	30.81567351260452
28	28.36537245109469	21.891245409196983	22.22620129661599	27.517180843092337
29	30.373518794708744	20.440340964404342	22.9726519756543	26.21348826523261
30	29.686571381248843	19.038766940441743	25.318835920069372	25.955825758240046
31	32.595280175059486	18.613904601936486	24.116469893627592	24.674345329376436
32	34.20289063779669	18.70763686850172	23.28870498490722	23.800767508794372
33	33.574341680530985	19.100577912921025	24.563932226398315	22.761148180149675
34	28.535952073006342	20.913827780411342	29.429023454340797	21.121196692241515
35	27.481282452042688	25.364155578580526	26.56964957159889	20.584912397777895
36	28.811458530558227	25.482269766570308	23.317984763339886	22.38828693953158
37	30.093107815580737	24.838139734602347	22.597406690289958	22.471345759526955
38	30.586219762574064	24.76035686169558	22.869974428949007	21.783448946781352
39	31.151688246739546	21.297265829510913	22.973791346612177	24.577254577137364
40	28.718812094398167	21.348041854683203	23.00676370114425	26.92638234977438
41	25.95702922645961	21.808908880144344	24.201799603954203	28.03226228944184
42	25.34507065662659	19.69606016225507	25.303617204746807	29.65525197637153
43	25.5212164848409	17.934034027766906	27.350756781292556	29.193992706099642
44	26.5941498585022	18.225621474786774	25.776280819317286	29.40394784739374
45	27.96991333348991	19.476282186783163	25.122523114983835	27.431281364743093
46	26.218521288634967	21.299990993495737	25.126188956459426	27.35529876140987
47	25.750018596311275	20.005324417543957	26.4942625485951	27.75039443754967
48	27.676823113184433	19.57015592383155	28.143516567519626	24.60950439546439
49	28.84791723813629	18.920216309416514	25.981229672009093	26.250636780438104
50	25.353641429142403	20.007288344135237	26.765856066958722	27.873214159763638
51	24.93619088474791	20.79481770083466	27.36612818237556	26.902863232041874
52	25.7950156092915	20.229955337632994	27.160363178141893	26.814665874933613
53	25.225122803166027	19.64184206709189	28.40350003724278	26.7295350924993
54	25.866280825167532	18.9905382567336	27.38788442008101	27.755296498017856
55	26.210811128757133	19.209654334058783	24.95029704135051	29.62923749583358
56	26.286479586334288	20.885647784115754	23.51086421544501	29.31700841410495
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	7.0
9	12.0
10	11.0
11	10.0
12	110.0
13	308.0
14	406.0
15	471.0
16	536.0
17	527.0
18	518.0
19	807.5
20	1097.0
21	1311.0
22	1544.0
23	1563.0
24	1810.5
25	2058.0
26	2603.5
27	3149.0
28	3789.0
29	4738.0
30	5047.0
31	5461.5
32	5876.0
33	6120.0
34	6364.0
35	5925.0
36	5486.0
37	5817.0
38	5925.5
39	5703.0
40	5840.5
41	5978.0
42	6794.5
43	7611.0
44	7760.5
45	7910.0
46	9064.5
47	10420.5
48	10622.0
49	11328.5
50	12035.0
51	13435.5
52	14836.0
53	16529.5
54	19945.0
55	21667.0
56	24258.5
57	26850.0
58	29374.5
59	31899.0
60	35698.0
61	39497.0
62	47090.5
63	54443.5
64	54203.0
65	47142.5
66	40082.0
67	35596.5
68	31111.0
69	27632.5
70	24154.0
71	20614.5
72	14739.5
73	12404.0
74	10653.5
75	8903.0
76	7594.5
77	6286.0
78	4873.0
79	2755.5
80	2051.0
81	1614.5
82	1178.0
83	915.0
84	652.0
85	490.5
86	329.0
87	239.0
88	108.0
89	67.0
90	46.5
91	26.0
92	19.5
93	13.0
94	8.5
95	4.0
96	3.5
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.051329911339244055
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	9.721574117281071E-4
24	5.832944470368642E-4
25	0.03752527609270493
26	0.054635246539119615
27	0.10421527453725309
28	0.10129880230206877
29	0.13960180432415617
30	0.21873541763882406
31	0.21834655467413283
32	0.2900917716596671
33	0.3136179810234873
34	0.3318945403639757
35	0.35639290713952404
36	0.3894462591382797
37	1.2888862964691243
38	0.46916316689998444
39	0.47207963913516876
40	0.6814823456214031
41	0.5372141857209519
42	0.5644345932493389
43	0.6237361953647534
44	0.6529009177165966
45	0.6602893140457303
46	0.6956758438326334
47	0.6738995178099237
48	0.8039741794991445
49	0.7660600404417484
50	0.7613936848654533
51	0.7413672421838544
52	0.7868642090527298
53	0.8076683776637114
54	0.829444703686421
55	0.8341110592627158
56	0.8444159278270337
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	514320.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.920067767725314
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.87744807874907	23.70285086810174
2	11.766570000626448	8.217788436311183
3	5.7718278771719165	6.046578618434684
4	3.557378635206248	4.968956120674415
5	2.437292371834769	4.255520739711504
6	1.7083189478463268	3.5792768056609763
7	1.19347561943379	2.9173374656829125
8	0.9196025645884253	2.5690067099841443
9	0.703522704971909	2.2110354480377237
>10	3.6967611240643503	23.01835382473711
>50	0.22916017165527827	5.540384597968534
>100	0.1277567956978167	8.900345575901666
>500	0.00802060600793468	1.9555593068947108
>1k	0.0028645021456909572	2.117005481898704
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	4871	0.9470757505055218	No Hit
AGGGTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC	2021	0.39294602582050087	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC	1542	0.2998133457769482	No Hit
AGGGTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT	1145	0.22262404728573654	No Hit
AGGGTCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG	1003	0.19501477679265827	No Hit
AGGGTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT	977	0.1899595582516721	No Hit
AGGGTCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA	919	0.17868253227562605	No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCG	918	0.17848810079328045	No Hit
AGGGTCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	841	0.1635168766526676	No Hit
AGGGTCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT	809	0.15729506921760772	No Hit
AGGGTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT	705	0.13707419505366308	No Hit
AGGGTCGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC	689	0.13396329133613316	No Hit
AGGGTCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA	618	0.12015865608959403	No Hit
AGGGTCGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT	608	0.1182143412661378	No Hit
AGGGTCGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG	571	0.11102037641934981	No Hit
AGGGTCGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCG	567	0.11024265048996734	No Hit
AGGGTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG	527	0.10246539119614247	No Hit
AGGGTCGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG	522	0.10149323378441438	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	5.832944470368642E-4	0.0	0.0	0.0	0.0
8	5.832944470368642E-4	0.0	0.0	0.0	0.0
9	5.832944470368642E-4	0.0	0.0	0.0	0.0
10	0.0011665888940737283	0.0	0.0	0.0	0.0
11	0.005249650023331778	0.0	0.0	0.0	0.0
12	0.005832944470368642	0.0	0.0	0.0	0.0
13	0.006416238917405506	0.0	0.0	0.0	0.0
14	0.010110437081972313	0.0	0.0	0.0	0.0
15	0.010888163011354798	0.0	0.0	0.0	0.0
16	0.011277025976046042	0.0	0.0	0.0	0.0
17	0.013026909317156634	0.0	0.0	0.0	0.0
18	0.014387929693575984	0.0	0.0	0.0	0.0
19	0.01691553896406906	0.0	0.0	0.0	0.0
20	0.018859853787525277	0.0	0.0	0.0	0.0
21	0.019832011199253385	0.0	0.0	0.0	0.0
22	0.02216518898740084	0.0	0.0	0.0	0.0
23	0.02352620936382019	0.0	0.0	0.0	0.0
24	0.026053818634313268	0.0	0.0	0.0	0.0
25	0.027220407528386996	0.0	0.0	0.0	0.0
26	0.028581427904806347	0.0	0.0	0.0	0.0
27	0.029942448281225695	0.0	0.0	0.0	0.0
28	0.03499766682221185	0.0	0.0	0.0	0.0
29	0.03674755016332244	0.0	0.0	0.0	0.0
30	0.03888629646912428	0.0	0.0	0.0	0.0
31	0.040636179810234874	0.0	0.0	0.0	0.0
32	0.04258049463369109	0.0	0.0	0.0	0.0
33	0.04433037797480168	0.0	0.0	0.0	0.0
34	0.04510810390418417	0.0	0.0	0.0	0.0
35	0.05132991133924405	0.0	0.0	0.0	0.0
36	0.05385752060973713	0.0	0.0	0.0	0.0
37	0.0554129724685021	0.0	0.0	0.0	0.0
38	0.05735728729195831	0.0	0.0	0.0	0.0
39	0.05988489656245139	0.0	0.0	0.0	0.0
40	0.06319023176232695	0.0	0.0	0.0	0.0
41	0.06435682065640068	0.0	0.0	0.0	0.0
42	0.06688442992689377	0.0	0.0	0.0	0.0
43	0.0690231762326956	0.0	0.0	0.0	0.0
44	0.0717452169855343	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACACC	15	0.008051934	50.58124	34
TCGTACC	15	0.008306555	50.18445	47
ACGTAGG	35	5.1346433E-8	50.17953	46
GTATTCG	15	0.008309775	50.179527	50
GTCTAGT	15	0.00832589	50.154938	45
ACGTATG	15	0.008455647	49.959095	29
ATAGTGC	15	0.008468705	49.939598	28
TCGACGG	15	0.008540792	49.83262	23
CTCCGTA	15	0.008577006	49.7793	20
TCGGTAC	25	2.1436097E-5	49.76962	5
GTCGGTA	40	2.7903297E-9	49.76962	4
ACAACGG	15	0.008593505	49.7551	12
ATTAACG	15	0.008593505	49.7551	14
ATCGGAT	15	0.008593505	49.7551	12
GATTAGT	15	0.008593505	49.7551	9
TCGGGAT	3400	0.0	49.257286	5
CGGGATA	370	0.0	49.097057	6
AGGGTCG	51860	0.0	49.001865	1
GGGTCGG	51795	0.0	48.775093	2
GGTCGGG	50855	0.0	48.72735	3
>>END_MODULE