FastQCFastQC Report
Fri 17 Jun 2016
SRR1526391_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526391_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences426444
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18290.4288957049460187No Hit
GGGTTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT15270.35807749669358696No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC15200.35643601504535183No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC13920.32642035062048No Hit
GGGTTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC13460.3156334712177918No Hit
GGGTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC13310.31211601054300214No Hit
GGGTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT9360.21948954610687454No Hit
GGGTTTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC9360.21948954610687454No Hit
GGGTTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8650.20284023224620348No Hit
GGGTTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC8460.1983847820581366No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC7720.18103197606250762No Hit
GGGTTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA7640.17915599703595314No Hit
GGGTTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG6920.1622721857969628No Hit
GGGTTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT6490.15218879852923245No Hit
GGGTTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6310.14796784571948485No Hit
GGGTTTGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC5280.12381461575259589No Hit
GGGTTTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC4930.11560720751142002No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTC4830.11326223372822691No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC4640.10880678354016002No Hit
GGGTTTGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC4550.10669630713528622No Hit
GGGTTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT4460.10458583073041244No Hit
GGGTTTGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC4370.10247535432553864No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAG150.00463739958.15043354
TCGAATC150.004639562758.14358558
AATTGCG150.00465906758.0820143
CAAACCG150.00466775558.05468838
GTATGGG150.00467863258.0205732
TGTATCG150.00469389257.9728727
GTTAATC150.00469389257.9728727
GCATAAT258.757106E-657.89128516
CTACGAA150.0047223357.88450210
TTGGGGC51550.056.3686145
GTTTGGG430700.056.1440623
TTTGGGG249100.056.130074
GGTTTGG436450.056.094052
GGGTTTG440950.055.771021
TTGGGGT39000.055.435545
TTGGGAC22650.055.201115
TTTGGGA124700.055.076154
TTGGGGG99250.054.851765
TTTGGGC38300.054.3328364
TTGGGAT23400.054.297645
Produced by FastQC (version 0.11.5)