FastQCFastQC Report
Fri 17 Jun 2016
SRR1526390_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526390_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences411833
Sequences flagged as poor quality0
Sequence length64
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14780.35888333377849757No Hit
GGGTTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT12440.30206418621140124No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC12170.2955081307228901No Hit
GGGTTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC10590.2571430652716028No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC10550.2561717977918234No Hit
GGGTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC9920.24087433498529742No Hit
GGGTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT9440.22921912522794433No Hit
GGGTTTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC7570.1838123705482562No Hit
GGGTTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7150.17361406201057222No Hit
GGGTTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC7070.1716715270510134No Hit
GGGTTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA6860.1665723727821714No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC6760.16414420408272284No Hit
GGGTTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG5730.13913406647840265No Hit
GGGTTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT5310.12893575794071868No Hit
GGGTTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5170.1255363217614907No Hit
GGGTTTGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACTTCTTGTGC4450.10805350712546107No Hit
GGGTTTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC4220.10246871911672936No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCGA150.00457320658.3551136
ATCGACG150.004593250358.2906935
ACGTTCG150.00464702258.11960641
ACCGTAC150.00464702258.11960641
CATATCG150.00465828358.08408745
TACCAGT150.00465828358.08408745
CGTGCTA201.9945014E-458.08408746
CCGATAC150.004662793658.06989329
ACCGATA150.004662793658.06989328
GTTGACG150.00466730758.05570627
CGACGCA202.0029518E-458.0344325
TCGTTAT150.00473083757.85780714
TATTCGG150.00473083757.85780715
TTCGTTA150.004735399557.84372313
CACGACA150.004735399557.84372312
ACATAAT150.004749106257.8015116
CGTATCA150.004753681457.78745317
CGTTTAT150.004753681457.78745320
GACCATA150.004762842357.759369
GTTTGGG415200.055.8891143
Produced by FastQC (version 0.11.5)