FastQCFastQC Report
Fri 17 Jun 2016
SRR1526389_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526389_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381701
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC29590.7752141073772403No Hit
GGGTTTGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT19760.5176826888061598No Hit
GGGTTTGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC15130.39638355676301607No Hit
GGGTTTGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG13390.35079813780943725No Hit
GGGTTTGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT12420.32538557666864903No Hit
GGGTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC11990.31412021451345423No Hit
GGGTTTGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA10210.26748685489427587No Hit
GGGTTTGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT7880.2064443111231042No Hit
GGGTTTGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA7690.2014665929615065No Hit
GGGTTTGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6210.16269278833432452No Hit
GGGTTTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG6180.1619068328351249No Hit
GGGTTTGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA6040.15823904050552656No Hit
GGGTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC5900.15457124817592827No Hit
GGGTTTGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT5830.15273735201112915No Hit
GGGTTTGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT5670.14854558934873108No Hit
GGGTTTGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA5280.13832816785913582No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC5080.13308846453113826No Hit
GGGTTTGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC5020.13151655353273897No Hit
GGGTTTGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT5020.13151655353273897No Hit
GGGTTTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC4780.1252289095391419No Hit
GGGTTTGGGGAGATTCTCGGACCTCGGCGACCTCCGGGGATCCGAGTTTGCAGACT4440.11632141388154603No Hit
GGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT4250.11134369571994833No Hit
GGGTTTGGGGACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA3840.10060230389755333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGCCG251.9378664E-550.62146836
GCCCAAT150.00802506350.62146436
TTGCGAT203.9434576E-450.59448235
ACTAGTG150.00804199850.5944835
CTACGGT150.00804199850.5944835
TACGGAC204.0908466E-450.21970748
CTATTGG204.0908466E-450.21970740
CGATTGT150.00829921750.1931544
TACCCTA150.00834702950.12026241
ACCGTAC204.1310594E-450.12026241
CGAGTAC150.00854033149.8308225
ACGCGAA204.2535862E-449.8242824
TAGGTAT150.00854476349.82427624
GCGCATA150.00857583249.7785522
TAATCAT252.1485996E-549.7459419
TACGCTT355.4929842E-849.7459419
TAAGTGG252.1519327E-549.73290613
CGGTATA355.504262E-849.73290614
CTGATAT252.1519327E-549.73290612
CGAGGTA150.00860698449.73290317
Produced by FastQC (version 0.11.5)