FastQCFastQC Report
Fri 17 Jun 2016
SRR1526388_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526388_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235227
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10520.44722757166481736No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9910.42129517444851144No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9840.41831932558762386No Hit
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6050.25719836583385414No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC4490.19087944836264545No Hit
GAGATCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC3800.16154608101961085No Hit
GAGATCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC3490.14836732177853734No Hit
GAGATCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG3470.14751707924685517No Hit
GAGATCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3280.13943977519587464No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC3260.13858953266419247No Hit
GAGATCGGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3130.13306295620825842No Hit
GAGATCGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3050.12966198608152973No Hit
GAGATCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG3050.12966198608152973No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC3010.12796150101816545No Hit
GAGATCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2950.12541077342311896No Hit
GAGATCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2910.12371028835975462No Hit
GAGATCGGGGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2730.11605810557461516No Hit
GAGATCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2720.11563298430877407No Hit
GAGATCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2650.11265713544788651No Hit
GAGATCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC2530.10755568025779354No Hit
GAGATCGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA2530.10755568025779354No Hit
GAGATCGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAGC2510.10670543772611137No Hit
GAGATCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCTC2470.10500495266274705No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGCGG150.004602955758.25176653
CGACGCT150.004602955758.25176657
ATGCGGG150.004602955758.25176654
CGAAAGA201.9645286E-458.25176257
ACTAGTA201.9645286E-458.25176258
TAGATAG201.9645286E-458.25176257
AACTAGT201.9645286E-458.25176257
CGTCAGT201.9707758E-458.2143851
TGTATCG150.004618585658.20192349
ACAATTA150.00464603258.1149143
TCCATTA150.004657832458.07769442
ATGTGGA150.004657832458.07769440
GCGTTCG150.004657832458.07769439
TTACGAA150.004657832458.07769441
ACCGTAG201.9959228E-458.06530438
TCGCTAC202.0022492E-458.0281533
ATAGGTA258.678007E-657.96634330
CGTATAG258.6999535E-657.94165427
ATTGATA150.00471716857.8923422
CGTAAAC202.0277148E-457.88002421
Produced by FastQC (version 0.11.5)