FastQCFastQC Report
Fri 17 Jun 2016
SRR1526387_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526387_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227857
Sequences flagged as poor quality0
Sequence length64
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8280.36338580776539675No Hit
GAGATCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8240.361630320771361No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7670.33661463110635176No Hit
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4410.19354244109243954No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC3660.16062705995426957No Hit
GAGATCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC3010.13210039630118892No Hit
GAGATCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT2950.1294671658101353No Hit
GAGATCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTCG2830.12420070482802811No Hit
GAGATCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2800.1228840895825013No Hit
GAGATCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2760.12112860258846557No Hit
GAGATCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2750.12068973083995665No Hit
GAGATCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT2750.12068973083995665No Hit
GAGATCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC2740.1202508590914477No Hit
GAGATCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.1123511676182869No Hit
GAGATCGGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2530.1110345523727601No Hit
GAGATCGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2530.1110345523727601No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCCGAAGGTCC2520.11059568062425117No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC2480.10884019363021544No Hit
GAGATCGGGGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2300.10094050215705465No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAT150.004593486458.28144535
TACCGTA150.00460558358.24275637
GTACGTA201.9702004E-458.21698439
ACTTCGG258.52469E-658.1398350
TCCGCTA201.9853315E-458.12698747
AAGTAGA258.547047E-658.11415557
TAGATAG150.004646075458.11415557
CACGTTG201.9875004E-458.1141532
TAGAGTT150.004650139258.10132244
GCACGTT201.9896714E-458.10132231
AAGCTAG150.004650139258.10132253
AACTACG150.004703209757.93506223
AAAATAG150.004703209757.93506223
TAGCGTT351.6507329E-857.92231422
ACTTACT150.004719629457.88409815
TTCGATA202.0268676E-457.88409813
CAACGAA150.004727855357.8586521
GAGTATT150.004744339357.8078169
GATAGTA150.004744339357.8078169
ATCGGCT202.0445618E-457.7824334
Produced by FastQC (version 0.11.5)