FastQCFastQC Report
Fri 17 Jun 2016
SRR1526386_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526386_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212034
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGATCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA11420.5385928671816784No Hit
GAGATCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT11380.5367063772791156No Hit
GAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7730.3645641736702604No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC6830.32211815086259754No Hit
GAGATCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC4700.22166256355112857No Hit
GAGATCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4550.2145882264165181No Hit
GAGATCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC3700.17450031598705867No Hit
GAGATCGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA3220.15186243715630512No Hit
GAGATCGGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3020.14242998764349113No Hit
GAGATCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.14195836516785043No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2930.13818538536272484No Hit
GAGATCGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA2920.13771376288708415No Hit
GAGATCGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG2830.13346916060631786No Hit
GAGATCGGGAGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA2830.13346916060631786No Hit
GAGATCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT2790.13158267070375507No Hit
GAGATCGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT2770.13063942575247367No Hit
GAGATCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2710.12780969089862948No Hit
GAGATCGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC2670.1259232009960667No Hit
GAGATCGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2600.12262184366658177No Hit
GAGATCGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAG2370.1117745267268457No Hit
GAGATCGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA2360.11130290425120501No Hit
GAGATCGGGGTGCAGTGCTGAGATCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2320.1094164143486422No Hit
GAGATCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2290.1080015469217201No Hit
GAGATCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTTCC2250.1061150570191573No Hit
GAGATCGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAAC2180.10281369968967241No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTAT150.0079640750.7112637
TAGACTG150.00799442350.66250236
CAATGCG309.531377E-750.66250236
GCACTCT150.00799442350.66250236
CTAATGC251.9256071E-550.662536
AACCTCC150.00800963150.63815734
TATCTCT203.9226396E-450.63815334
ATTGTAA150.00807833650.52889333
ATGCGTT150.00807833650.52889333
GTATAGA150.00807833650.52889333
AGATCAC251.955942E-550.5288933
AATTGTA150.00808599650.5167832
TAGGTAG150.00811669450.4683931
CAATATC203.9882096E-450.46838831
GCTTACC204.073791E-450.2517940
GTCTTGA150.00825590650.2517940
TACGTAG204.073791E-450.2517940
CAGATTG150.00826369150.2398148
CCTAGAA150.00827148350.22783750
TAGAGTT150.008287081550.20390744
Produced by FastQC (version 0.11.5)