FastQCFastQC Report
Fri 17 Jun 2016
SRR1526384_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526384_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284597
Sequences flagged as poor quality0
Sequence length64
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15760.553765499987702No Hit
AAGTACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8190.2877753454885329No Hit
AAGTACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT7590.2666929025956001No Hit
AAGTACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT7580.2663415285473846No Hit
AAGTACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC7300.25650305519734923No Hit
AAGTACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7000.24596183375088285No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC6480.22769038324367438No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTC4160.14617160405766752No Hit
AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3930.13809000094870993No Hit
AAGTACGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTTCTTCTAGC3930.13809000094870993No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCAAAGGTCC3760.13211664212904564No Hit
AAGTACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC3590.12614328330938135No Hit
AAGTACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.1191158023450704No Hit
AAGTACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGT3260.11454793971826828No Hit
AAGTACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3030.10646633660931072No Hit
AAGTACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC2870.10084435183786197No Hit
AAGTACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2870.10084435183786197No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGGT150.00461061958.2304739
AGGTAAC150.004617091358.20984634
ATTGCGA303.6590245E-758.20984634
AACCGTT150.004643046858.127546
ATGAATC150.004643046858.127552
ACTAATT150.00465932458.07614540
ACCGCGA303.7267455E-758.0556328
CGCTAGA150.00466584758.0556328
GTTCGAA303.740406E-758.02488724
CGACGGA202.008772E-457.9941829
TACGGTC407.366907E-1057.810594
ACGGACT407.366907E-1057.810595
TACGGTT150.004744652757.8105854
AACTAGA150.004744652757.81058517
ACGAGAC150.004744652757.8105855
ACTGGAT202.0422862E-457.80042310
GAATTAG150.00475126457.7902579
ACTAGAT150.004761194357.75979618
ACGGGGC30900.057.1557735
TACGGGG143200.056.740774
Produced by FastQC (version 0.11.5)