FastQCFastQC Report
Fri 17 Jun 2016
SRR1526383_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526383_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260303
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT19280.7406752899505575No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC13830.5313039035278119No Hit
AAGTACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA11870.4560070379519252No Hit
AAGTACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG9950.3822468431020772No Hit
AAGTACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8670.33307337986884517No Hit
AAGTACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC7840.30118746230354626No Hit
AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC6320.24279397471408318No Hit
AAGTACGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC5530.2124447278748228No Hit
AAGTACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC5120.19669385293292815No Hit
AAGTACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC4550.17479629508687952No Hit
AAGTACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4340.1667287737751774No Hit
AAGTACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.15520374332988865No Hit
AAGTACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA3970.15251456955932124No Hit
AAGTACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT3960.15213040187781163No Hit
AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3940.15136206651479236No Hit
AAGTACGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT3900.14982539578875387No Hit
AAGTACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT3810.14636788665516723No Hit
AAGTACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC3520.1352270238913881No Hit
AAGTACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3430.1317695147578015No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT3130.12024448431251272No Hit
AAGTACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG3070.11793947822345498No Hit
AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC2850.10948778923024322No Hit
AAGTACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG2710.10410944168910846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTA150.00804598950.5835136
TTACGGG150.0081396150.43572231
GATAGCA150.00827203850.23026350
TGCCATA252.0294132E-550.22051646
TGCGAAA451.3096724E-1050.1913244
GTCGAAC150.00829744350.19131545
GAATTAG150.00829744350.19131544
TACGCCT204.1076844E-450.17186440
CATACGC204.1116143E-450.16214838
TGCGTCG204.1116143E-450.16214839
CGATTGG150.00831653550.16214439
TCATGTA150.00836120950.09421541
CACGAAC150.00845109449.958930
CCGAGGT150.00845109449.958930
CAGCCGT402.7121132E-949.91075527
TCTTAAC150.00848336949.91075527
ATGACCT252.1123047E-549.88191226
AGCTAGG150.00850277949.8819126
GCCAGTA1250.049.87230325
ATAACAC150.00855469749.80515323
Produced by FastQC (version 0.11.5)