##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526383_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 260303 Sequences flagged as poor quality 0 Sequence length 56 %GC 48 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.28170631917419 39.0 38.0 40.0 35.0 40.0 2 38.16207266147528 39.0 38.0 40.0 35.0 40.0 3 38.11778581115085 39.0 38.0 40.0 35.0 40.0 4 37.51156536805185 39.0 37.0 40.0 33.0 40.0 5 37.983104305367206 39.0 38.0 40.0 35.0 40.0 6 38.29286254864523 39.0 38.0 40.0 35.0 40.0 7 38.27982773921161 39.0 38.0 40.0 36.0 40.0 8 38.44269946946443 39.0 38.0 40.0 36.0 40.0 9 38.41860447248015 39.0 38.0 40.0 36.0 40.0 10 37.759365047656004 39.0 38.0 40.0 34.0 40.0 11 38.03075262290484 39.0 38.0 40.0 35.0 40.0 12 37.90038147850774 39.0 38.0 40.0 35.0 40.0 13 37.880957960530615 39.0 38.0 40.0 35.0 40.0 14 37.9173693733841 39.0 38.0 40.0 35.0 40.0 15 37.90816855741194 39.0 38.0 40.0 35.0 40.0 16 37.84968671125573 39.0 38.0 40.0 35.0 40.0 17 37.74525072703734 39.0 38.0 40.0 33.0 40.0 18 37.681590300534374 39.0 38.0 40.0 33.0 40.0 19 37.55533743368306 39.0 38.0 40.0 33.0 40.0 20 37.537170144024465 39.0 38.0 40.0 33.0 40.0 21 37.39769422557558 39.0 38.0 40.0 33.0 40.0 22 37.30817163075339 39.0 37.0 40.0 33.0 40.0 23 37.12373656853744 39.0 36.0 40.0 33.0 40.0 24 37.00070302685716 39.0 36.0 40.0 33.0 40.0 25 36.92910953773103 39.0 36.0 40.0 32.0 40.0 26 36.560016596043845 39.0 36.0 40.0 31.0 40.0 27 36.41004521653611 38.0 36.0 40.0 31.0 40.0 28 36.178764747236876 38.0 35.0 40.0 30.0 40.0 29 36.0958114197685 38.0 35.0 40.0 30.0 40.0 30 35.870762150263346 38.0 35.0 40.0 29.0 40.0 31 35.313046718631746 38.0 35.0 40.0 28.0 40.0 32 35.09151258341241 38.0 35.0 40.0 27.0 40.0 33 34.95730360387702 38.0 34.0 39.0 27.0 40.0 34 35.1222267895491 38.0 35.0 40.0 28.0 40.0 35 34.94378858484151 38.0 34.0 40.0 27.0 40.0 36 34.39853555279809 38.0 34.0 40.0 25.0 40.0 37 33.85909881945271 38.0 33.0 40.0 21.0 40.0 38 33.9528703088324 38.0 33.0 40.0 23.0 40.0 39 33.785426983169614 38.0 33.0 40.0 22.0 40.0 40 33.391947077060195 38.0 33.0 39.0 18.0 40.0 41 32.908541200063006 38.0 32.0 39.0 13.0 40.0 42 32.74372173966493 37.0 32.0 39.0 11.0 40.0 43 32.54147282205737 37.0 32.0 39.0 10.0 40.0 44 32.1155076967995 37.0 31.0 39.0 4.0 40.0 45 31.971033756814172 36.0 31.0 39.0 4.0 40.0 46 31.342109003737953 36.0 30.0 39.0 4.0 40.0 47 31.08441316465811 36.0 30.0 39.0 4.0 40.0 48 30.66731462948948 36.0 29.0 39.0 4.0 40.0 49 30.582743956081952 36.0 29.0 39.0 4.0 40.0 50 30.169867423733113 35.0 29.0 39.0 4.0 40.0 51 29.424420771178205 35.0 28.0 39.0 4.0 40.0 52 29.146721320922158 35.0 27.0 39.0 4.0 40.0 53 28.794043864265873 35.0 26.0 39.0 4.0 40.0 54 28.43845441658375 35.0 24.0 39.0 4.0 40.0 55 27.88599055715839 35.0 20.0 39.0 4.0 40.0 56 16.7039142845069 20.0 4.0 24.0 4.0 27.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.6263739917554005 1 2 -0.1477346344059285 1 3 -1.175346684560182 1 4 -0.16714047825583123 1 5 -0.5692130265802504 1 6 -0.6150361891924732 1 7 -1.9959306538456119 1 8 -2.4034016676836387 1 9 -2.5262529628654065 1 10 -0.6413671957564375 1 11 -0.5669588101234169 1 12 -0.7885268512332999 1 13 -0.9557389721859195 1 14 -0.9202605678960651 1 15 -1.1619081036280932 1 16 -0.930015331011866 1 17 -0.9576134157010614 1 18 -0.9101905571555307 1 19 -1.1674754055731498 1 20 -1.1226466201530698 1 21 -0.7721342251402206 1 22 -0.6668567820692815 1 23 -1.4106893133408391 1 24 -1.132070981016028 1 25 -0.9898750846010103 1 26 -0.6577246070782721 1 27 -0.48214352913578296 1 28 -0.6981260257446067 1 29 -0.5902892440472272 1 30 -0.5775430142785822 1 31 -0.21625978134940027 1 32 -0.5426487077553546 1 33 -0.5625436360836389 1 34 -0.4246278147076623 1 35 0.09230173854464852 1 36 -0.020936375709183608 1 37 0.13163212208220898 1 38 0.3244555233364821 1 39 0.1628308552642892 1 40 -0.5729452660880767 1 41 -0.16998957824337424 1 42 -0.2411841015955858 1 43 -0.2162321815689836 1 44 0.19959415200530728 1 45 0.06859957440362408 1 46 0.2270055317451245 1 47 0.24752851375550122 1 48 0.423452865412262 1 49 0.2992058708944043 1 50 0.732726747694624 1 51 1.1328700998742427 1 52 0.9735811154751417 1 53 1.016469744670463 1 54 1.1730292330492595 1 55 0.30180021291930714 1 56 1.539961662398877 2 1 0.26378470671750165 2 2 0.6917536711468557 2 3 0.2261238547484652 2 4 0.46103265944920224 2 5 0.753041356758068 2 6 0.35319084610712537 2 7 0.5286892350321821 2 8 0.2534503135520154 2 9 0.34241323801958146 2 10 0.7160703395930597 2 11 0.10587946382275248 2 12 0.27832945511796225 2 13 0.2647920189309545 2 14 0.12432139304481638 2 15 0.14835240141233186 2 16 0.10543398196015374 2 17 0.3922218972044149 2 18 0.06575514600845622 2 19 0.06766116826987911 2 20 0.08840238673384704 2 21 -0.10154168430114652 2 22 0.05329160266639121 2 23 0.10125465977689885 2 24 -0.07474656407488567 2 25 -0.11500400631641838 2 26 -0.01609370579071623 2 27 -0.23669321806015375 2 28 0.33822158047025397 2 29 0.3379471068859985 2 30 0.3835309451984088 2 31 0.36638319699174815 2 32 0.2718038324183141 2 33 0.755252551160595 2 34 0.4915481176874579 2 35 0.7418176582971157 2 36 1.3237254335199466 2 37 1.2508389624189036 2 38 1.5644828048349666 2 39 1.1326881264490396 2 40 1.4080075901517333 2 41 1.3747920034181362 2 42 0.8441870265941915 2 43 1.1015806407353068 2 44 1.082333613694761 2 45 1.4074890177945107 2 46 0.8907111577224356 2 47 1.0611825710466505 2 48 1.10010056811047 2 49 1.144765973366372 2 50 0.5580752522027232 2 51 1.1836236680946222 2 52 1.0918064872687339 2 53 1.129754503628444 2 54 1.3350713530213127 2 55 1.2617928934928848 2 56 0.5003535844137161 3 1 0.28806377648494674 3 2 0.8117846788987961 3 3 0.30782928110505026 3 4 0.5236063028600526 3 5 0.7524522094712509 3 6 0.5290668150993696 3 7 0.5501465993732708 3 8 0.359310778668295 3 9 0.2823822302676433 3 10 0.8273881690504297 3 11 0.2807321770010489 3 12 0.429430230311759 3 13 0.6201408561402602 3 14 0.3198252690138048 3 15 0.24798030838907437 3 16 0.06782157885938034 3 17 0.3006870134834898 3 18 0.1095690994968237 3 19 0.18313403648693338 3 20 -0.2766208690801051 3 21 0.028380796319005697 3 22 0.2290745484028207 3 23 0.16057248923426215 3 24 0.10751700181658919 3 25 0.04279444329598192 3 26 0.19590629420928707 3 27 0.08690368116464953 3 28 0.20098127039273095 3 29 0.017109897583672762 3 30 0.38874024752399094 3 31 0.6446157551312837 3 32 0.6505015068369175 3 33 0.32911301627687095 3 34 0.6383078076099338 3 35 0.6993060303901402 3 36 0.8079734955354496 3 37 1.417846714356891 3 38 1.3095370684008572 3 39 1.3557578938909032 3 40 0.8104882103067723 3 41 0.8401563445034128 3 42 0.9038459413228708 3 43 1.163596144611276 3 44 0.9574653966404973 3 45 0.9819386301976181 3 46 1.1284475918309624 3 47 1.3884383850001392 3 48 1.782457157257756 3 49 1.3773861284051314 3 50 1.4103233142182283 3 51 1.7286469239085775 3 52 2.033666952385012 3 53 2.2255684571168146 3 54 1.9053039111608499 3 55 1.348924676438621 3 56 0.02720629759200932 4 1 0.2133971098182812 4 2 0.7721338852480031 4 3 0.3017975350733053 4 4 0.1524316996854509 4 5 0.7059760189950595 4 6 0.4602414182739736 4 7 0.5486227898494604 4 8 0.2905488739063884 4 9 0.22235048423589632 4 10 0.35643578809804666 4 11 -0.14596623569736522 4 12 -0.21936342048189061 4 13 0.029188475187879703 4 14 -0.17763504844651123 4 15 -0.40351175510298987 4 16 -0.44338477034696666 4 17 -0.3584240976276263 4 18 -0.059383281455552606 4 19 -0.41388183652893673 4 20 -0.22287483733407498 4 21 -0.22114301320480223 4 22 -0.14032227699399868 4 23 -0.6671100504482723 4 24 -0.09191156961198033 4 25 -0.2704119059103647 4 26 -0.1510143407113489 4 27 -0.09909631883535042 4 28 0.19840984182130228 4 29 -0.48311232463854736 4 30 0.2802640570478019 4 31 0.610107818623348 4 32 0.6021840465194543 4 33 0.3677479369117904 4 34 0.5779268552289878 4 35 0.16355999864411075 4 36 0.042163971725926785 4 37 0.4643863968965718 4 38 0.11883865570244723 4 39 0.34032932246233116 4 40 0.4455675753861428 4 41 0.34406110640816934 4 42 0.3727030841800172 4 43 0.26677074778587695 4 44 0.20867174584684633 4 45 0.3487957730547606 4 46 -0.0921238367404662 4 47 0.26300981357156417 4 48 0.12169525249585078 4 49 0.21665596967497436 4 50 0.21698998088489674 4 51 0.5425199397815916 4 52 0.2677939365119961 4 53 0.4728700444184035 4 54 0.16609756195450132 4 55 0.25717864469259055 4 56 0.3080316944174051 5 1 0.033289582936561146 5 2 0.5294943563181462 5 3 0.20911960368569993 5 4 0.09834823834392381 5 5 0.5093554352776977 5 6 -0.07035253973933919 5 7 0.4791143413087511 5 8 0.30014948834570987 5 9 0.17402739155796354 5 10 0.289807523889138 5 11 -0.09617104880540239 5 12 0.06852700450531302 5 13 0.21256021097897104 5 14 -0.08194892453457925 5 15 0.07933514709875311 5 16 0.07114415950454145 5 17 0.006719271548007555 5 18 0.28124651885166685 5 19 -0.06102725383564689 5 20 0.2211855825327973 5 21 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-0.11841552448742831 120 50 0.781728272895915 120 51 0.7311923784540291 120 52 0.7692041424676574 120 53 0.4640808538110299 120 54 0.8355518450451456 120 55 0.09884203181052342 120 56 -0.04747138835840303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 136.0 5 20.0 6 36.0 7 61.0 8 127.0 9 121.0 10 134.0 11 199.0 12 271.0 13 313.0 14 497.0 15 678.0 16 808.0 17 962.0 18 1264.0 19 1546.0 20 1460.0 21 1886.0 22 2088.0 23 2287.0 24 3002.0 25 4107.0 26 4088.0 27 4068.0 28 4849.0 29 5278.0 30 6183.0 31 7803.0 32 9292.0 33 11329.0 34 15069.0 35 19793.0 36 27358.0 37 39721.0 38 47848.0 39 35621.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 100.0 0.0 0.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 99.2419466441147 0.5447066963942492 0.14146228953640347 0.0718843699546398 8 99.52017456579448 0.40414440094812587 0.0334225882913374 0.04225844496605878 9 97.6250753929075 1.277741708701014 0.5109430164078017 0.5862398819836881 10 51.11427836021867 31.257419238349154 6.874680660614745 10.753621740817431 11 34.57509133586628 28.020422353949055 18.08738278083618 19.31710352934849 12 37.69222790363538 16.78620684356308 23.5840539678759 21.937511284925645 13 26.52485756983208 16.959082300242407 28.08534669212418 28.43071343780133 14 25.286300964645047 20.818814996369618 30.037302681874582 23.857581357110753 15 29.38383345562671 20.007837020702794 27.731144089772304 22.877185433898187 16 29.728431865940845 20.23987430033461 24.99817520351283 25.033518630211717 17 30.357698528253614 20.109641456302846 22.488407740210448 27.044252275233095 18 29.820632109503155 22.198745308352187 23.003192433433345 24.97743014871131 19 28.05346077455888 22.976300695727673 24.155311310280712 24.81492721943274 20 26.771109053679748 24.65741847001379 23.199886286366272 25.37158618994019 21 26.53407759418831 21.33667303104459 27.121469979216528 25.00777939555057 22 26.624741167024585 20.908710233842868 26.80837331878618 25.658175280346367 23 29.10158200859002 23.302932747347313 25.62369862697943 21.971786617083232 24 27.638772036987945 23.8416294981579 25.01296575887146 23.50663270598269 25 27.022207190240827 20.111766198027563 26.265825217344513 26.600201394387092 26 25.985108533955543 18.889175049683065 28.209602958304664 26.916113458056728 27 22.606785997246217 17.734975346722766 29.393552456481775 30.26468619954924 28 22.978939433564605 22.24051197637032 29.399796932449274 25.380751657615804 29 26.07760527885189 19.943440872626535 30.273753871606935 23.705199976914642 30 23.865544276890375 20.363980838249564 33.25079712265676 22.519677762203294 31 26.841233778736186 18.637991451345837 33.20035426855096 21.320420501367014 32 27.26873384017232 17.698262541567605 35.27244843305063 19.760555185209448 33 26.75725161414667 18.254216054736435 34.81314445408114 20.175387877035753 34 22.845995782396326 20.65006611691321 38.78846057465814 17.71547752603233 35 22.061857260188404 24.32877430698815 36.907291228854895 16.702077203968553 36 23.354561479978553 23.044818262120682 35.11786057312138 18.482759684779378 37 22.8331743776398 23.241917589582886 35.61866204721957 18.30624598555774 38 24.51374786697655 22.2012029866189 35.57381226305101 17.711236883353536 39 23.962057276988055 20.4666012925543 35.452973106531495 20.118368323926152 40 22.676152783742065 20.264775633213787 36.81441332698862 20.244658256055523 41 20.451384596365436 20.941247372975646 36.76172889108666 21.84563913957226 42 19.846741222205907 19.560008965213427 37.49507299693173 23.098176815648934 43 19.9808980248403 18.833232796621964 38.997030300218086 22.188838878319643 44 21.17853675142259 18.362365429176002 37.53128541973718 22.927812399664226 45 21.95154975782616 20.119771598347338 36.4316884081519 21.496990235674605 46 20.46609365990333 20.461449750970385 36.47132579730111 22.60113079182517 47 20.87902308412589 20.31836437785843 37.51770195708195 21.284910580933737 48 21.812370455029733 21.529938205838484 37.13344826918234 19.52424306994944 49 22.741744323859542 21.531376604463446 35.331117876259746 20.395761195417258 50 19.414083983361348 21.712355825464574 36.29441427763619 22.579145913537882 51 19.6571499378502 23.6711365475688 36.28657835328193 20.38513516129907 52 19.99984503451856 22.843849032627983 36.21969456303608 20.936611369817374 53 21.27282802019599 21.4258866745973 36.8452867621701 20.455998543036607 54 20.97976901015425 21.405704984109757 35.54336873110612 22.071157274629876 55 21.336325511809804 22.33010085036782 33.57828887700286 22.755284760819517 56 20.514738657725925 22.65431500610477 33.013818097249946 23.81712823891936 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 3.0 5 5.0 6 9.5 7 14.0 8 89.5 9 165.0 10 174.5 11 184.0 12 266.5 13 517.0 14 685.0 15 1414.0 16 2143.0 17 2650.5 18 3158.0 19 3390.0 20 3622.0 21 4240.5 22 5693.0 23 6527.0 24 7304.5 25 8082.0 26 8552.5 27 9023.0 28 9305.0 29 9367.5 30 9148.0 31 7578.0 32 6008.0 33 5976.5 34 5945.0 35 4967.0 36 3989.0 37 3738.0 38 3344.0 39 3201.0 40 3600.5 41 4000.0 42 4359.0 43 4718.0 44 5915.5 45 7113.0 46 6577.5 47 6233.5 48 6425.0 49 7001.5 50 7578.0 51 9041.5 52 10505.0 53 11155.0 54 12255.5 55 12706.0 56 14262.0 57 15818.0 58 17212.0 59 18606.0 60 18657.5 61 18709.0 62 16894.5 63 13189.5 64 11299.0 65 10084.0 66 8869.0 67 7660.5 68 6452.0 69 5610.0 70 4768.0 71 4016.5 72 2983.0 73 2701.0 74 2137.5 75 1574.0 76 1267.5 77 961.0 78 736.0 79 420.0 80 329.0 81 246.0 82 163.0 83 119.5 84 76.0 85 51.5 86 27.0 87 22.0 88 10.5 89 4.0 90 2.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.06261933208606893 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 3.841676815096253E-4 24 7.683353630192506E-4 25 0.044947618736626165 26 0.0591618229524823 27 0.1140978014083587 28 0.11179279531930096 29 0.15289873724083086 30 0.23779979485445807 31 0.23549478876540034 32 0.3019557976665655 33 0.337299224365451 34 0.3511292608997975 35 0.37033764497527877 36 0.40568107167416434 37 1.3130851353998994 38 0.49211880001383007 39 0.49211880001383007 40 0.6991851803475181 41 0.5589639765965049 42 0.5858557143021785 43 0.6496275494327763 44 0.689965155991287 45 0.6957276712139314 46 0.7299185948682881 47 0.7133993845633743 48 0.8405588871430602 49 0.8132829817558769 50 0.8102096403037998 51 0.7898487531837896 52 0.8378697133724928 53 0.8574622651294838 54 0.8770548168864747 55 0.8824331644276093 56 0.8866590089242152 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 260303.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.27468830522753 #Duplication Level Percentage of deduplicated Percentage of total 1 69.72301707066461 27.383497631504106 2 11.747536222648295 9.227616469977635 3 5.575704750226523 6.569521984413695 4 3.2984456901789847 5.181817054940029 5 2.089983487273941 4.104172501287826 6 1.5432479228192992 3.6366348687850687 7 1.0664802030024148 2.9319974289630935 8 0.8305951689558166 2.6097093094853996 9 0.6656755504082911 2.352977977922677 >10 3.187446441767694 21.415868132042128 >50 0.17391523388757604 4.689468622589104 >100 0.08895664262065672 6.426134384043497 >500 0.00599707703060607 1.7048687617532625 >1k 0.002998538515303035 1.765714872292465 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAGTACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT 1928 0.7406752899505575 No Hit AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 1383 0.5313039035278119 No Hit AAGTACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA 1187 0.4560070379519252 No Hit AAGTACGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 995 0.3822468431020772 No Hit AAGTACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 867 0.33307337986884517 No Hit AAGTACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 784 0.30118746230354626 No Hit AAGTACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 632 0.24279397471408318 No Hit AAGTACGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC 553 0.2124447278748228 No Hit AAGTACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC 512 0.19669385293292815 No Hit AAGTACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC 455 0.17479629508687952 No Hit AAGTACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 434 0.1667287737751774 No Hit AAGTACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 404 0.15520374332988865 No Hit AAGTACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA 397 0.15251456955932124 No Hit AAGTACGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT 396 0.15213040187781163 No Hit AAGTACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC 394 0.15136206651479236 No Hit AAGTACGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 390 0.14982539578875387 No Hit AAGTACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT 381 0.14636788665516723 No Hit AAGTACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC 352 0.1352270238913881 No Hit AAGTACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 343 0.1317695147578015 No Hit AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCT 313 0.12024448431251272 No Hit AAGTACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG 307 0.11793947822345498 No Hit AAGTACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGTTCTTCC 285 0.10948778923024322 No Hit AAGTACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 271 0.10410944168910846 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.009220024356231009 0.0 0.0 0.0 0.0 11 0.013061701171327262 0.0 0.0 0.0 0.0 12 0.013445868852836886 0.0 0.0 0.0 0.0 13 0.014214204215856136 0.0 0.0 0.0 0.0 14 0.017671713349442765 0.0 0.0 0.0 0.0 15 0.018440048712462017 0.0 0.0 0.0 0.0 16 0.020360887120010142 0.0 0.0 0.0 0.0 17 0.023434228572087143 0.0 0.0 0.0 0.0 18 0.024970899298125647 0.0 0.0 0.0 0.0 19 0.027660073068693024 0.0 0.0 0.0 0.0 20 0.034959259017375906 0.0 0.0 0.0 0.0 21 0.03764843278794328 0.0 0.0 0.0 0.0 22 0.048020960188703166 0.0 0.0 0.0 0.0 23 0.052246804685309046 0.0 0.0 0.0 0.0 24 0.05954599063399192 0.0 0.0 0.0 0.0 25 0.061082661360030426 0.0 0.0 0.0 0.0 26 0.06338766744908818 0.0 0.0 0.0 0.0 27 0.06646100890116519 0.0 0.0 0.0 0.0 28 0.07683353630192506 0.0 0.0 0.0 0.0 29 0.07952271007249244 0.0 0.0 0.0 0.0 30 0.08451688993211758 0.0 0.0 0.0 0.0 31 0.08605356065815607 0.0 0.0 0.0 0.0 32 0.08720606370268495 0.0 0.0 0.0 0.0 33 0.08835856674721382 0.0 0.0 0.0 0.0 34 0.09066357283627158 0.0 0.0 0.0 0.0 35 0.10564611241514696 0.0 0.0 0.0 0.0 36 0.10756695082269509 0.0 0.0 0.0 0.0 37 0.11140862763779134 0.0 0.0 0.0 0.0 38 0.11486613677137797 0.0 0.0 0.0 0.0 39 0.1198603166310031 0.0 0.0 0.0 0.0 40 0.12754367026119562 0.0 0.0 0.0 0.0 41 0.13253785012082073 3.841676815096253E-4 0.0 0.0 0.0 42 0.13830036534346513 3.841676815096253E-4 0.0 0.0 0.0 43 0.14137370679554212 3.841676815096253E-4 0.0 0.0 0.0 44 0.1459837189736576 3.841676815096253E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGCGTA 15 0.008045989 50.58351 36 TTACGGG 15 0.00813961 50.435722 31 GATAGCA 15 0.008272038 50.230263 50 TGCCATA 25 2.0294132E-5 50.220516 46 TGCGAAA 45 1.3096724E-10 50.19132 44 GTCGAAC 15 0.008297443 50.191315 45 GAATTAG 15 0.008297443 50.191315 44 TACGCCT 20 4.1076844E-4 50.171864 40 CATACGC 20 4.1116143E-4 50.162148 38 TGCGTCG 20 4.1116143E-4 50.162148 39 CGATTGG 15 0.008316535 50.162144 39 TCATGTA 15 0.008361209 50.094215 41 CACGAAC 15 0.008451094 49.9589 30 CCGAGGT 15 0.008451094 49.9589 30 CAGCCGT 40 2.7121132E-9 49.910755 27 TCTTAAC 15 0.008483369 49.910755 27 ATGACCT 25 2.1123047E-5 49.881912 26 AGCTAGG 15 0.008502779 49.88191 26 GCCAGTA 125 0.0 49.872303 25 ATAACAC 15 0.008554697 49.805153 23 >>END_MODULE