FastQCFastQC Report
Fri 17 Jun 2016
SRR1526382_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526382_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173255
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10510.6066202995584543No Hit
ATCAACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8850.5108077688955586No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC6220.359008398026031No Hit
ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT4180.24126287841620733No Hit
ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC4150.2395313266572393No Hit
ATCAACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3930.22683328042480735No Hit
ATCAACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC3710.2141352341923754No Hit
ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3550.20490029147787941No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3500.20201437187959945No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA3130.1806585668523275No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC2420.13967850855675162No Hit
ATCAACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC2300.1327523015208796No Hit
ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2190.12640327840466364No Hit
ATCAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2170.12524891056535165No Hit
ATCAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG2160.12467172664569566No Hit
ATCAACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC2090.12063143920810367No Hit
ATCAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACA2080.12005425528844767No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC2060.11889988744913567No Hit
ATCAACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1970.11370523217223169No Hit
ATCAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCG1960.11312804825257569No Hit
ATCAACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1930.11139649649360768No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC1900.1096649447346397No Hit
ATCAACGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCCC1890.1090877608149837No Hit
ATCAACGGGGCAAAGATGGGGTTTAATGAGGTGACCTGTAGCTTTGGCACGTCCAGAGTTCTTT1880.1085105768953277No Hit
ATCAACGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.10677902513635969No Hit
ATCAACGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG1830.10562465729704772No Hit
ATCAACGGGGAGTCCCGCCCGGGAGCCGGCAGGGAGCGGAGCTGTGGAGCCTCCTGGTCTCCAG1800.1038931055380797No Hit
ATCAACGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCTGGGGAAAT1800.1038931055380797No Hit
ATCAACGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC1760.10158436985945572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCG150.00461050558.22109257
TCCGAAT201.9681861E-458.22109257
TTTAAGT150.00461050558.22109258
CTAGTAG201.9681861E-458.22109258
TACCGGA150.00461050558.22109258
CCTTCGT351.585795E-858.20418555
ACATTGC201.9710157E-458.20418556
GGCTAGT201.9710157E-458.20418556
ATCATTT150.004615812658.2041855
GATCATT150.004621124458.18728354
CTTAGGT150.004621124458.18728354
ATTCGTA150.00462644158.1703950
TCAAGGA150.00462644158.1703948
GCACGTC150.00463176258.15351547
CATTCTT150.00463176258.15351547
ACTTGGA150.004637087758.13664246
CTTCGTA150.004642417758.1197840
CGCATTC150.004642417758.1197845
AGGACTA150.004642417758.1197843
GCGCATT150.004642417758.1197844
Produced by FastQC (version 0.11.5)