FastQCFastQC Report
Fri 17 Jun 2016
SRR1526381_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526381_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167424
Sequences flagged as poor quality0
Sequence length64
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8910.5321817660550459No Hit
ATCAACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7230.43183772935779813No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC5210.31118597094801226No Hit
ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT3730.22278765290519878No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT3410.20367450305810397No Hit
ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC3340.199493501529052No Hit
ATCAACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT3120.18635321100917432No Hit
ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3000.17918577981651376No Hit
ATCAACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC2990.17858849388379205No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA2440.14573776758409787No Hit
ATCAACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC2060.12304090214067277No Hit
ATCAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC2030.12124904434250765No Hit
ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1910.11408161314984709No Hit
ATCAACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1850.11049789755351683No Hit
ATCAACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC1830.10930332568807338No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC1750.1045250382262997No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC1740.103927752293578No Hit
ATCAACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA1720.10273318042813455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATAGG201.9702734E-458.2074137
TAAGACG150.004614516658.20740534
TTGATAG201.9732086E-458.18988836
TTATCAG201.9761472E-458.17238657
ACCGCTA150.0046255358.1723857
CCGCTAA150.0046255358.1723858
ATGACGC150.0046255358.1723858
TGAATTA201.9820347E-458.13740553
ATCGAAC201.9820347E-458.13740552
CCGAATC150.004636562458.137452
AACATCG258.53209E-658.1199349
ACGTCCA150.00464208658.11992649
CGACTGT201.9879363E-458.10246744
GTTATGT201.9938518E-458.0675741
GGATAAG150.004664229758.05013331
TACAACT150.004664229758.05013330
CTTAATA150.00467533158.015327
AACCTAC202.017654E-457.92840214
TCGCAAC150.0047031757.92839415
TTAATGA150.004714340457.8937123
Produced by FastQC (version 0.11.5)