##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526381_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 167424 Sequences flagged as poor quality 0 Sequence length 64 %GC 50 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.189811496559635 39.0 38.0 40.0 35.0 40.0 2 38.16372204701835 39.0 38.0 40.0 35.0 40.0 3 38.165657253440365 39.0 38.0 40.0 35.0 40.0 4 38.020242020259936 39.0 38.0 40.0 35.0 40.0 5 38.30355862958716 39.0 38.0 40.0 36.0 40.0 6 38.023592794342505 39.0 38.0 40.0 35.0 40.0 7 38.2869779720948 39.0 38.0 40.0 36.0 40.0 8 38.153156058868504 39.0 38.0 40.0 36.0 40.0 9 38.34025587729358 39.0 38.0 40.0 36.0 40.0 10 37.493471664755354 39.0 38.0 39.0 33.0 40.0 11 37.192421636085626 39.0 37.0 39.0 33.0 40.0 12 36.912485665137616 39.0 36.0 39.0 32.0 40.0 13 36.84672448394495 39.0 36.0 39.0 32.0 40.0 14 36.87565701452599 39.0 36.0 39.0 32.0 40.0 15 36.66946793769113 39.0 36.0 39.0 31.0 40.0 16 36.38001720183486 38.0 36.0 39.0 31.0 40.0 17 36.0057936735474 38.0 35.0 39.0 30.0 40.0 18 36.24522768539755 38.0 35.0 39.0 30.0 40.0 19 36.1423153191896 38.0 35.0 39.0 30.0 40.0 20 36.02714067278288 38.0 35.0 39.0 30.0 40.0 21 36.017906632263 38.0 35.0 39.0 30.0 40.0 22 35.894788082951074 38.0 35.0 39.0 30.0 40.0 23 35.70719849006116 38.0 35.0 39.0 29.0 40.0 24 35.56046325496942 38.0 35.0 39.0 29.0 40.0 25 35.457210435779814 38.0 35.0 39.0 29.0 40.0 26 35.73454224006116 38.0 35.0 39.0 29.0 40.0 27 35.16628440366973 38.0 35.0 39.0 27.0 40.0 28 35.05434704701835 38.0 35.0 39.0 27.0 40.0 29 34.94780318233945 38.0 35.0 39.0 27.0 40.0 30 34.91358467125382 38.0 34.0 39.0 27.0 40.0 31 34.53977924311926 38.0 34.0 39.0 25.0 40.0 32 34.63485521788991 38.0 34.0 39.0 26.0 40.0 33 34.50703005542813 38.0 33.0 39.0 26.0 40.0 34 33.927871750764524 38.0 33.0 39.0 23.0 40.0 35 33.890469705657495 38.0 33.0 39.0 23.0 40.0 36 33.21655198776758 38.0 33.0 39.0 19.0 40.0 37 32.92919175267584 37.0 32.0 39.0 18.0 40.0 38 32.91501218463303 37.0 32.0 39.0 19.0 40.0 39 32.42989655007645 37.0 31.0 39.0 13.0 40.0 40 32.461684107415905 36.0 31.0 39.0 15.0 40.0 41 31.873823346712538 36.0 31.0 39.0 4.0 40.0 42 31.620783161314986 36.0 30.0 39.0 4.0 40.0 43 31.449618931574925 36.0 30.0 39.0 4.0 40.0 44 31.335477589831804 36.0 30.0 39.0 4.0 40.0 45 31.053612385321102 36.0 30.0 39.0 4.0 40.0 46 30.6082341838685 35.0 29.0 39.0 4.0 40.0 47 30.376433486238533 35.0 29.0 39.0 4.0 40.0 48 30.162509556574925 35.0 28.0 39.0 4.0 40.0 49 29.924735999617738 35.0 28.0 39.0 4.0 40.0 50 29.475224579510705 35.0 27.0 39.0 4.0 40.0 51 29.053606412461775 35.0 26.0 39.0 4.0 39.0 52 28.831583285550458 35.0 25.0 38.0 4.0 39.0 53 28.566824350152906 34.0 25.0 38.0 4.0 39.0 54 28.288220326834864 34.0 23.0 38.0 4.0 39.0 55 27.941955753058103 34.0 22.0 38.0 4.0 39.0 56 27.200383457568808 34.0 16.0 38.0 4.0 39.0 57 26.79569237385321 33.0 14.0 38.0 4.0 39.0 58 26.44862146406728 33.0 10.0 37.0 4.0 39.0 59 26.11849555619266 33.0 4.0 37.0 4.0 39.0 60 25.748554568042813 33.0 4.0 37.0 4.0 39.0 61 25.38953196674312 33.0 4.0 37.0 4.0 39.0 62 25.11322152140673 32.0 4.0 37.0 4.0 39.0 63 24.822791236620795 32.0 4.0 36.0 4.0 39.0 64 24.624366876911314 32.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 2 1 -0.3575578739873464 2 2 0.1255322178685887 2 3 -0.2652487841332132 2 4 -0.06983580549410817 2 5 -0.30175353965313434 2 6 -0.09355103939353882 2 7 -0.19608397033773173 2 8 0.24059477443550037 2 9 -0.1347811910556942 2 10 -0.002720230227474474 2 11 -0.7620680985627075 2 12 -0.9212088755862027 2 13 -0.1005566544859775 2 14 -0.804387194290662 2 15 -0.5580872135663455 2 16 -0.547045485895346 2 17 -0.5324689772125524 2 18 -0.8496249407710224 2 19 -0.3675931713381715 2 20 -0.31767996291307554 2 21 0.028546644646070263 2 22 -0.655370491083552 2 23 0.37992554973830295 2 24 -0.422200800346765 2 25 -0.47209164671389203 2 26 -0.2738292393447779 2 27 0.22404288170718445 2 28 -0.37207147211959324 2 29 0.13627838814402082 2 30 -0.44793869955297083 2 31 -0.5738386901395813 2 32 -1.1471866253111287 2 33 -0.4578404833458336 2 34 -0.7624098488466231 2 35 -1.4053737049200237 2 36 -1.532336075445997 2 37 -1.8549128072240677 2 38 -2.2190322957835313 2 39 -4.4107023224408834 2 40 -2.462225005245653 2 41 -3.342448061201331 2 42 -2.608242531471916 2 43 -3.1108486004302662 2 44 -2.479036733718143 2 45 -2.328447493846621 2 46 -1.1827392393042864 2 47 -0.7590947368326404 2 48 -1.6602356898124917 2 49 -2.213864029589942 2 50 -1.5364007315558936 2 51 -1.3318080289088208 2 52 -0.8950258967461373 2 53 -1.7198699358155451 2 54 -0.9729018437898915 2 55 -1.536066818311422 2 56 -0.7996744665881046 2 57 -1.2700545086655453 2 58 -1.203553349073836 2 59 -0.46164713275369706 2 60 -0.8121978077917262 2 61 -0.360625633593326 2 62 -0.6510063840502127 2 63 -0.18020218781725106 2 64 -1.5920663574761242 3 1 -0.43770027905063813 3 2 -0.2875215795997619 3 3 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0.4650851886020746 4 21 0.49956937191879547 4 22 0.6247431452800853 4 23 0.495834640647395 4 24 0.8304507148047477 4 25 0.4379462320739833 4 26 1.0161328818673425 4 27 1.2958231847374861 4 28 1.311830043031918 4 29 0.9468844487500832 4 30 1.223273421659151 4 31 0.818964340163447 4 32 0.7880406474161461 4 33 0.09670497119962107 4 34 1.030393181456411 4 35 -3.5625706746169925 4 36 0.38508816697824244 4 37 0.5604281018668402 4 38 0.14744497694373848 4 39 0.5302067684682115 4 40 0.28550226748161833 4 41 0.876112544859275 4 42 1.5383483776189948 4 43 1.0891513995697366 4 44 1.3347890238576134 4 45 1.2637873546382252 4 46 0.8487001546351074 4 47 1.0753749601370544 4 48 1.3632491586723567 4 49 0.9588632431373334 4 50 0.8601901775350136 4 51 0.9852374256366332 4 52 0.6941786487084087 4 53 1.327667942972333 4 54 1.3028557319676857 4 55 1.3612816665370637 4 56 1.6510831091694733 4 57 1.7521045822435468 4 58 2.5551587721382845 4 59 1.5875952914887286 4 60 1.9018173437234225 4 61 2.6160789118612193 4 62 2.676266343222512 4 63 2.342714478849416 4 64 2.1115321273723637 5 1 -0.8749920845136643 5 2 -0.15769146634193731 5 3 -0.5057093104489994 5 4 -0.09384896338885085 5 5 -0.4971482764952384 5 6 -0.5593405130777498 5 7 -0.5220708124429905 5 8 -0.0765104887223984 5 9 -0.36241277000306127 5 10 -0.3484439144380005 5 11 -1.7025286248784965 5 12 -1.6468667703230437 5 13 -1.7449645492228214 5 14 -1.7162292995538166 5 15 -1.6505214240926591 5 16 -18.592440222737455 5 17 -3.295626871949395 5 18 -3.062124940771021 5 19 -3.6929221187065906 5 20 -3.2219562787025495 5 21 -2.6500717764065627 5 22 -3.087936280557237 5 23 -2.8187586607880135 5 24 -2.70838501087308 5 25 -3.289525857240207 5 26 -0.4221845025026738 5 27 -0.2203650130296566 5 28 0.016415369985665507 5 29 -0.571616348698079 5 30 -0.24662291007928872 5 31 -1.9929176375080004 5 32 -1.9281076779427124 5 33 -1.7683667991353076 5 34 -1.5656993225308327 5 35 -6.256031599656865 5 36 -2.2836518649196833 5 37 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0.7981122139524501 119 1 -0.24235725670340003 119 2 0.2135723413253814 119 3 0.10288393191616763 119 4 0.29382160191329376 119 5 0.10827732454439598 119 6 -0.08305721223304374 119 7 -0.1643710073747684 119 8 0.1220762559169799 119 9 0.006576833635669743 119 10 -0.07903195862253654 119 11 -0.09856501214295577 119 12 0.11166149478417253 119 13 0.04597112329179964 119 14 -0.07506620663633612 119 15 0.36174303334723845 119 16 0.252182909166379 119 17 0.40117299809608653 119 18 -0.1686064222525019 119 19 0.2226074459457763 119 20 0.39752065437087225 119 21 0.0673583730411309 119 22 -0.1634723429354068 119 23 -0.44075346260737547 119 24 -0.26247857812454356 119 25 -0.7494836220225309 119 26 -0.33470886897440266 119 27 0.1816817705960716 119 28 -0.21937085483564545 119 29 -0.11742531555967872 119 30 0.32428352266924776 119 31 0.740513161712272 119 32 -0.13530390926175073 119 33 0.19524593640725385 119 34 0.4445345955978226 119 35 0.08120036915405393 119 36 0.18093552949227387 119 37 0.11923842734383072 119 38 0.593236222734987 119 39 0.6615198997813394 119 40 0.21154042685311225 119 41 0.2614099634900313 119 42 0.7724673450712913 119 43 0.6311267082117098 119 44 0.5648675872695108 119 45 0.5313518888694304 119 46 0.16216816810312196 119 47 0.041368226130320096 119 48 0.30735690278009997 119 49 -0.0027529184788299688 119 50 -0.08717233649416656 119 51 -0.5100487696495613 119 52 0.6339092884390496 119 53 -0.31284833087727293 119 54 -0.2074697450244578 119 55 0.27766774958981344 119 56 -0.13331644189674563 119 57 0.27724487405050624 119 58 0.16851455216072964 119 59 0.034957805517908724 119 60 0.24906762430703822 119 61 0.5138342429498834 119 62 0.6131911468139819 119 63 0.1624675652691714 119 64 -0.21158796241439504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 139.0 5 18.0 6 44.0 7 77.0 8 218.0 9 131.0 10 295.0 11 320.0 12 412.0 13 582.0 14 733.0 15 912.0 16 1127.0 17 1242.0 18 1350.0 19 1579.0 20 1745.0 21 2308.0 22 2830.0 23 2564.0 24 2863.0 25 2756.0 26 2994.0 27 3299.0 28 3849.0 29 4591.0 30 5324.0 31 6128.0 32 7547.0 33 9411.0 34 11494.0 35 14473.0 36 17713.0 37 21508.0 38 22493.0 39 12322.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 0.0 100.0 4 0.0 100.0 0.0 0.0 5 0.0 100.0 0.0 0.0 6 0.0 0.0 0.0 100.0 7 98.88365925625067 0.9706012352020642 0.07406434040926521 0.0716751681379986 8 99.12693243138263 0.4326495001225043 0.16911575764457007 0.27130231085029965 9 98.44227828746178 0.990897362385321 0.2245795107033639 0.34224483944954126 10 56.92015481651376 27.553994648318042 5.462179854740061 10.063670680428134 11 34.601371368501525 26.790065940366976 18.143754778287462 20.464807912844037 12 36.61661410550459 16.266485091743117 24.343582759938837 22.773318042813457 13 25.633720374617734 16.537055619266056 29.657635703363916 28.171588302752294 14 24.983873279816514 20.17631880733945 31.603593272171253 23.236214640672785 15 29.01947423161066 18.642183409245778 28.16889622111442 24.169446138029148 16 28.812111291006747 20.312938744540897 25.28542666132954 25.589523303122814 17 30.797892382313712 20.00681805948458 21.46372971776822 27.731559840433484 18 30.325401376146786 21.932339449541285 21.935923165137613 25.806336009174313 19 28.211009174311926 21.889334862385322 23.865156727828747 26.03449923547401 20 27.794570957490023 23.821357738536168 21.636120337403522 26.74795096657029 21 27.95835722477064 21.091360856269112 26.594753440366976 24.35552847859327 22 29.260440558103973 19.519304281345565 25.92758505351682 25.292670107033636 23 30.481890290519875 22.10913608562691 24.32088589449541 23.0880877293578 24 29.632837372102326 23.699819016957456 22.41322669470012 24.254116916240093 25 27.4137549822819 20.832063486372302 23.945429447302846 27.808752084042954 26 27.749069554879835 18.199088372871028 24.995668875042565 29.056173197206576 27 24.907986474759866 17.679763009066697 26.55734761662527 30.854902899548158 28 24.65315792307554 20.80932809256301 25.874209547056253 28.663304437305193 29 26.378298857173615 20.938660542887387 26.909345341681767 25.773695258257234 30 25.706762417713946 19.913225613405146 29.643925792938358 24.73608617594255 31 27.26559928175969 19.29373036061649 30.264851114768813 23.175819242855006 32 28.846878645568484 18.558822649636564 31.119619514821572 21.47467918997338 33 27.801289380271406 18.822318130937347 31.97172222654543 21.40467026224582 34 24.400743449847113 21.211703339528746 35.75873853348522 18.628814677138916 35 23.76461712561298 24.821421086981253 33.843473262560394 17.57048852484537 36 25.586106050436285 23.320188896346725 30.882634960207117 20.211070093009877 37 24.931068380166874 24.365229692145395 31.622830152488728 19.080871775199 38 26.479859055335165 22.74230856814123 31.863561729208868 18.91427064731474 39 25.904776788666023 21.00596873177974 31.52488173736378 21.564372742190457 40 25.13637944057453 21.095351709089165 32.018895296557844 21.749373553778458 41 21.984922362762916 21.64786460113832 33.89829491954395 22.468918116554814 42 21.341913640647604 19.241459212524685 34.87390670116399 24.542720445663726 43 21.50030313398522 19.276320133499006 37.40853457228095 21.814842160234825 44 23.991839918399183 18.74058740587406 35.24675246752468 22.020820208202082 45 22.258637662368844 19.474657254675982 33.810535667122856 24.456169415832314 46 23.11211440784813 20.214094451315425 33.6511329114684 23.02265822936804 47 22.924521505247235 20.49902740087894 35.11563122883696 21.46081986503686 48 23.784939006819712 21.678993372394583 33.908846412448376 20.627221208337335 49 24.27637385399593 21.112291888062344 33.03613777865836 21.575196479283367 50 20.445625191587578 22.11296304087803 33.70919560265187 23.732216164882523 51 20.9778365066971 23.782209141690192 33.19899093038621 22.0409634212265 52 22.229831612209466 23.74309898414644 33.12127164921513 20.905797754428963 53 22.891993390416104 21.855490461168696 33.371789094186575 21.880727054228633 54 22.989251575614475 21.24090529490576 31.696737021082296 24.073106108397468 55 23.34589425983689 21.931738275967763 31.05433652465007 23.668030939545282 56 22.359531651960665 23.071790926357803 30.465462938475223 24.103214483206308 57 22.525484444871857 24.331033322113765 30.030172621051115 23.113309611963263 58 22.848894903022103 25.851150202976996 29.94436926777928 21.35558562622162 59 24.4914775119387 25.070669890416564 28.99269844706675 21.445154150577988 60 24.99623931212506 24.05576648835992 28.42598665407087 22.522007545444154 61 23.646004292387325 23.531179925574573 28.534756915011933 24.28805886702617 62 22.71743443598716 23.364356745187163 27.64029676659091 26.27791205223477 63 22.65553130467721 24.122609234173296 27.520938929874877 25.700920531274612 64 23.16353919668757 23.234554230209074 27.850531409106775 25.75137516399658 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.5 3 1.0 4 2.5 5 14.5 6 25.0 7 90.0 8 155.0 9 158.0 10 175.0 11 189.0 12 223.0 13 638.0 14 1019.0 15 1259.5 16 1575.0 17 1650.0 18 1893.0 19 2136.0 20 2468.5 21 3109.5 22 3418.0 23 3711.0 24 4142.0 25 4280.0 26 4316.5 27 3673.5 28 2994.0 29 3087.0 30 2702.0 31 2224.0 32 2096.0 33 1968.0 34 1925.0 35 1913.0 36 1944.0 37 1950.0 38 1993.0 39 2030.0 40 1987.0 41 1956.5 42 1969.0 43 2210.0 44 2451.0 45 2514.5 46 2757.0 47 2936.0 48 3401.5 49 4076.0 50 4285.0 51 4337.5 52 5479.0 53 6568.0 54 7046.0 55 7858.5 56 8193.0 57 9398.0 58 10603.0 59 11176.5 60 11801.0 61 11852.0 62 11021.5 63 9417.5 64 8644.0 65 7636.5 66 5792.0 67 4955.0 68 4289.0 69 3623.0 70 3118.5 71 2385.0 72 2156.0 73 1759.5 74 1105.0 75 847.0 76 706.0 77 446.5 78 328.0 79 278.0 80 175.0 81 122.0 82 90.5 83 59.0 84 44.5 85 23.0 86 16.0 87 10.0 88 5.0 89 6.0 90 4.0 91 1.0 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.001194571865443425 8 0.04957473241590214 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.07525802752293578 16 0.025683295107033637 17 0.13200019113149847 18 0.0 19 0.0 20 0.01672400611620795 21 0.0 22 0.0 23 0.0 24 0.0029864296636085627 25 0.04838016055045872 26 0.018515863914373088 27 0.19650707186544344 28 0.16305905963302753 29 0.1236381880733945 30 0.19352064220183487 31 0.20845279051987767 32 0.1606699159021407 33 0.21920393730886853 34 0.3786792813455658 35 0.24548451834862386 36 0.334480122324159 37 0.3535932721712538 38 0.3285072629969419 39 0.6313312308868502 40 0.36434441896024466 41 0.4103354357798165 42 0.5023174694189603 43 0.49634461009174313 44 0.45334002293577985 45 0.494552752293578 46 0.5148604740061162 47 0.5136659021406728 48 0.5064984709480123 49 0.5178469036697247 50 0.6277475152905199 51 0.5578650611620795 52 0.5751863532110092 53 0.5966886467889908 54 0.5859375 55 0.6181909403669724 56 0.6283448012232417 57 0.6259556574923547 58 0.6862815366972477 59 0.6916571100917431 60 0.7352589831804281 61 0.6468606651376146 62 0.6558199541284403 63 0.6611955275229358 64 0.7537748470948011 >>END_MODULE >>Sequence Length Distribution pass #Length Count 64 167424.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.26058390672783 #Duplication Level Percentage of deduplicated Percentage of total 1 80.58449495912349 42.91977255351682 2 8.02727344091689 8.550745412844037 3 3.6031893777124853 5.7572391055045875 4 2.14419486155813 4.568042813455658 5 1.3165715311031614 3.506068425076453 6 0.9577104664072401 3.060493119266055 7 0.6627715288602797 2.470971903669725 8 0.4934339639568918 2.102446483180428 9 0.36558970965896986 1.7524369266055047 >10 1.6765540366262575 15.322773318042811 >50 0.1099012010631259 3.874593845565749 >100 0.05495060053156295 4.839210626911315 >500 0.0033643224815242626 1.2752054663608563 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCAACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 891 0.5321817660550459 No Hit ATCAACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 723 0.43183772935779813 No Hit ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC 521 0.31118597094801226 No Hit ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGT 373 0.22278765290519878 No Hit ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT 341 0.20367450305810397 No Hit ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC 334 0.199493501529052 No Hit ATCAACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTGAGACGCGT 312 0.18635321100917432 No Hit ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 300 0.17918577981651376 No Hit ATCAACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC 299 0.17858849388379205 No Hit ATCAACGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAAAAAAAAAA 244 0.14573776758409787 No Hit ATCAACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACAC 206 0.12304090214067277 No Hit ATCAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCAGCTCGCTC 203 0.12124904434250765 No Hit ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG 191 0.11408161314984709 No Hit ATCAACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA 185 0.11049789755351683 No Hit ATCAACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC 183 0.10930332568807338 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCCGAAGGTCC 175 0.1045250382262997 No Hit ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCCGAAGGTCC 174 0.103927752293578 No Hit ATCAACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA 172 0.10273318042813455 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.001194571865443425 0.0 0.0 0.0 0.0 9 0.001194571865443425 0.0 0.0 0.0 0.0 10 0.0035837155963302754 0.0 0.0 0.0 0.0 11 0.005375573394495413 0.0 0.0 0.0 0.0 12 0.005972859327217125 0.0 0.0 0.0 0.0 13 0.006570145259938838 0.0 0.0 0.0 0.0 14 0.008362003058103976 0.0 0.0 0.0 0.0 15 0.008959288990825688 0.0 0.0 0.0 0.0 16 0.010153860856269114 0.0 0.0 0.0 0.0 17 0.01194571865443425 0.0 0.0 0.0 0.0 18 0.014932148318042814 0.0 0.0 0.0 0.0 19 0.016724006116207953 0.0 0.0 0.0 0.0 20 0.0238914373088685 0.0 0.0 0.0 0.0 21 0.025086009174311925 0.0 0.0 0.0 0.0 22 0.03523987003058104 0.0 0.0 0.0 0.0 23 0.040615443425076454 0.0 0.0 0.0 0.0 24 0.04658830275229358 0.0 0.0 0.0 0.0 25 0.04838016055045872 0.0 0.0 0.0 0.0 26 0.04897744648318043 0.0 0.0 0.0 0.0 27 0.050769304281345566 0.0 0.0 0.0 0.0 28 0.059728593272171254 0.0 0.0 0.0 0.0 29 0.06152045107033639 0.0 0.0 0.0 0.0 30 0.06988245412844037 0.0 0.0 0.0 0.0 31 0.07346616972477064 0.0 0.0 0.0 0.0 32 0.07764717125382263 0.0 0.0 0.0 0.0 33 0.08003631498470948 0.0 0.0 0.0 0.0 34 0.08123088685015291 0.0 0.0 0.0 0.0 35 0.09855217889908256 0.0 0.0 0.0 0.0 36 0.10213589449541284 0.0 0.0 0.0 0.0 37 0.10631689602446483 0.0 0.0 0.0 0.0 38 0.11049789755351681 0.0 0.0 0.0 0.0 39 0.1188599006116208 0.0 0.0 0.0 0.0 40 0.1242354740061162 0.0 0.0 0.0 0.0 41 0.13080561926605505 0.0 0.0 0.0 0.0 42 0.13618119266055045 0.0 0.0 0.0 0.0 43 0.14095948012232415 0.0 0.0 0.0 0.0 44 0.1481269113149847 0.0 0.0 0.0 0.0 45 0.15469705657492355 0.0 0.0 0.0 0.0 46 0.16066991590214066 0.0 0.0 0.0 0.0 47 0.16783734709480122 0.0 0.0 0.0 0.0 48 0.1767966360856269 0.0 0.0 0.0 0.0 49 0.18038035168195718 0.0 0.0 0.0 0.0 50 0.18695049694189603 0.0 0.0 0.0 0.0 51 0.19352064220183487 0.0 0.0 0.0 0.0 52 0.19829892966360857 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATAGG 20 1.9702734E-4 58.20741 37 TAAGACG 15 0.0046145166 58.207405 34 TTGATAG 20 1.9732086E-4 58.189888 36 TTATCAG 20 1.9761472E-4 58.172386 57 ACCGCTA 15 0.00462553 58.17238 57 CCGCTAA 15 0.00462553 58.17238 58 ATGACGC 15 0.00462553 58.17238 58 TGAATTA 20 1.9820347E-4 58.137405 53 ATCGAAC 20 1.9820347E-4 58.137405 52 CCGAATC 15 0.0046365624 58.1374 52 AACATCG 25 8.53209E-6 58.11993 49 ACGTCCA 15 0.004642086 58.119926 49 CGACTGT 20 1.9879363E-4 58.102467 44 GTTATGT 20 1.9938518E-4 58.06757 41 GGATAAG 15 0.0046642297 58.050133 31 TACAACT 15 0.0046642297 58.050133 30 CTTAATA 15 0.004675331 58.0153 27 AACCTAC 20 2.017654E-4 57.928402 14 TCGCAAC 15 0.00470317 57.928394 15 TTAATGA 15 0.0047143404 57.89371 23 >>END_MODULE