FastQCFastQC Report
Fri 17 Jun 2016
SRR1526380_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526380_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154871
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCAACGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT11320.7309309037844399No Hit
ATCAACGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA10080.650864267680844No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC4240.2737762395800376No Hit
ATCAACGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCT3750.24213700434555213No Hit
ATCAACGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3580.23116012681522038No Hit
ATCAACGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC3410.2201832492848887No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC3370.21760045457186952No Hit
ATCAACGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCTG3190.205977878363283No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC3010.19435530215469649No Hit
ATCAACGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG2890.18660691801563883No Hit
ATCAACGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC2820.1820870272678552No Hit
ATCAACGGGGGGCTGGAGAGATGGCTCAGCCGTTAAAGGCTAGGCTCACAACCAAA2740.17692143784181674No Hit
ATCAACGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC2600.16788165634624946No Hit
ATCAACGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTT2390.15432198410289855No Hit
ATCAACGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC2210.14269940789431204No Hit
ATCAACGGGGGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT2060.13301392772048995No Hit
ATCAACGGGGATAGTAGAGGCCACAGGAAGACCGTCGTGGAGAAGCCCCGATGGCT1950.12591124225968708No Hit
ATCAACGGGGGCAAGCTCCGTTGGGCGCCTTGGATCCATTTCCATCTGGTTCTGCT1890.12203705019015827No Hit
ATCAACGGGGGGCTGGTGAGATGGCTCAGTGGGTTAGAGCACCCGACTGCTCTTCC1890.12203705019015827No Hit
ATCAACGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA1890.12203705019015827No Hit
ATCAACGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1870.12074565283364866No Hit
ATCAACGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGC1810.11687146076411982No Hit
ATCAACGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1770.1142886660511006No Hit
ATCAACGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG1740.11235157001633618No Hit
ATCAACGGGGGGGATGCGGGCCTTCCCTGGGGCTTGGACCTTTGCAGGGAGGCCAT1710.11041447398157177No Hit
ATCAACGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1710.11041447398157177No Hit
ATCAACGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.10912307662506214No Hit
ATCAACGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1660.10718598059029773No Hit
ATCAACGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG1640.10589458323378813No Hit
ATCAACGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGC1560.10072899380774968No Hit
ATCAACGGGGAATCCGCAGCGGCTTCTGAGCAGATCAGACTCTCCTCGTTCGCGCA1550.10008329512949488No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTACG203.9067704E-450.67094437
TCGCCAA150.00803712150.58771536
GTCTAGC150.00803712150.58771536
ATACCCA203.9835332E-450.4716533
CGTACCC150.00811045950.47164533
GGTAGCA150.00811045950.47164533
TGCCATT150.00813150450.43858332
CGTTAAA203.9964428E-450.43858331
CCCGTAC150.00813150450.43858331
CCGTACC150.00813150450.43858332
CCATACC203.9964428E-450.43858331
TAGATTG150.00813150450.43858331
ATGCAGG251.9742321E-550.43858332
CACTGAT150.0082160950.30676348
CTTGATA150.0082160950.30676347
CATGCCG150.00824797750.25750745
TAAGGAG150.00824797750.25750743
AACCCCT204.10092E-450.17562539
CCCCGTA150.00847377649.91539830
CCTACTA252.1282895E-549.80239523
Produced by FastQC (version 0.11.5)