FastQCFastQC Report
Fri 17 Jun 2016
SRR1526379_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526379_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20078
Sequences flagged as poor quality0
Sequence length64
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5612.794102998306604No Hit
GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3261.623667695985656No Hit
GCCCTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2371.1803964538300629No Hit
GCCCTCGGGGCGATACTGCTAATTACACACATGTGATGTTTTTGGGTTTGTTTGTTGATTTTTT1780.8865424843111863No Hit
GCCCTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1560.7769698177109273No Hit
GCCCTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1400.6972806056380118No Hit
GCCCTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1240.6175913935650961No Hit
GCCCTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.6026496663014245No Hit
GCCCTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT960.47813527243749376No Hit
GCCCTCGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATG700.3486403028190059No Hit
GCCCTCGGGAGCCCTGAGTTTGATTGTTTCCTGCTGTCTACTTCTCTTGGGTGTATTTGCTTCT680.3386791513098914No Hit
GCCCTCGGGTCTGAAATGCGGTAGGTGAGGGAAAGAGCAGTTTCCGCAAACTCTGAACCCTGCC650.32373742404621975No Hit
GCCCTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC580.28887339376431914No Hit
GCCCTCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.2789122422552047No Hit
GCCCTCGGGGCCTTATTATGTGCTTAAGATACAATAGATTAGAATTTTATCTAAATATCTAGAA540.26895109074609025No Hit
GCCCTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.2589899392369758No Hit
GCCCTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.25400936348241854No Hit
GCCCTCGGGAACTTAGTGATGAAAATCCAAACCAGAGAACATTAAGTGTGGTTATTCACAATCT500.24902878772786133No Hit
GCCCTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG490.24404821197330412No Hit
GCCCTCGGGGCCCACCTGTTTTTCCCTAGATTAGGTCTTGTTGTATTGCCAAGGGTGATCTTGT470.23408706046418964No Hit
GCCCTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG440.219145333200518No Hit
GCCCTCGGGGCTTCCTGTGTGCTACGGGATGGAACCCAGAGCCTCACACATCCTAAGCAACAAT440.219145333200518No Hit
GCCCTCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA420.20918418169140351No Hit
GCCCTCGGGGGTCGAGACTCCTCTACCCTGTGCAAAGGTGTATGAGTTTCGACCCCAGAGCTCT420.20918418169140351No Hit
GCCCTCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA420.20918418169140351No Hit
GCCCTCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCATCAAGC380.18926187867317462No Hit
GCCCTCGGGTCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC380.18926187867317462No Hit
GCCCTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC330.1643589999003885No Hit
GCCCTCGGGGCTCCCCGCGCACTGCCTGGTGTGGTTTGGGCCACGCATAAAAGGTTCTCCGGCC320.15937842414583125No Hit
GCCCTCGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCAGCCGGA320.15937842414583125No Hit
GCCCTCGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.15439784839127402No Hit
GCCCTCGGGATGGCACTGGCCCCAACATTCTCACCGACATCACCAAGGGTGTGCAGTACCTGAA300.1494172726367168No Hit
GCCCTCGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATGC290.14443669688215957No Hit
GCCCTCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA280.13945612112760236No Hit
GCCCTCGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA260.1294949696184879No Hit
GCCCTCGGGGCCCCGCCTCACCGGAGCCTTTCTGGTCACTCCTGTCCACGCGGTTCTATGGTAC260.1294949696184879No Hit
GCCCTCGGGGTGCTCGCTTCGGCAGCACATATACTAAAATTGGAACGATACAGAGAAGATTAGC260.1294949696184879No Hit
GCCCTCGGGAATGTCAAAGGCGTCTATGCTGTGGGAGACGTCTGTGGGAAAGCGCTTCTCACCC260.1294949696184879No Hit
GCCCTCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACTGAGAAGC250.12451439386393066No Hit
GCCCTCGGGGGTTTTAAAGTAAATTACCACTACATGTCTCAGGTGAAAAATGCCAACGATGCCA250.12451439386393066No Hit
GCCCTCGGGGCTTTTTTTCCGACATCTTAGCGAGGCTGCTGTGGTCTACACGACTCTCTGAGCT240.11953381810937344No Hit
GCCCTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.11455324235481622No Hit
GCCCTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGGGGCCTTCTCCTCTCCCC230.11455324235481622No Hit
GCCCTCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230.11455324235481622No Hit
GCCCTCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.11455324235481622No Hit
GCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAAAGGAAGGA220.109572666600259No Hit
GCCCTCGGGGGGGCTGGAGAGATAGCTCAGTGGTTAAGAGCACTGACTGCTCTTCCAAAGGTCC220.109572666600259No Hit
GCCCTCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.10459209084570176No Hit
GCCCTCGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.10459209084570176No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATG150.004567562658.16792358
AGCCCAT150.004567562658.16792353
GGTGATT150.004567562658.16792351
GCCCATC150.004567562658.16792354
GTGCAGC150.004567562658.16792349
TAAATAT150.004567562658.16792352
TCTAGAA150.004567562658.16792358
GTCGCGT150.004567562658.16792344
ATCTAGA150.004567562658.16792357
TTATCTA150.004567562658.16792347
TCGCGTG150.004567562658.16792345
AAATATC150.004567562658.16792353
CCGTCAT150.004567562658.16792357
CCCATCA150.004567562658.16792355
TTTTATC150.004567562658.16792345
TTTTAGG150.004567562658.16792356
GCGTGCA150.004567562658.16792347
CCCGTCA150.004567562658.16792356
CCCCGTC150.004567562658.16792355
GCAGCCC150.004567562658.16792351
Produced by FastQC (version 0.11.5)