FastQCFastQC Report
Fri 17 Jun 2016
SRR1526378_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526378_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19244
Sequences flagged as poor quality0
Sequence length64
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4412.2916233631261695No Hit
GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2681.3926418623986696No Hit
GCCCTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC2151.1172313448347537No Hit
GCCCTCGGGGCGATACTGCTAATTACACACATGTGATGTTTTTGGGTTTGTTTGTTGATTTTTT1290.6703388069008522No Hit
GCCCTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1110.5768031594263148No Hit
GCCCTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1110.5768031594263148No Hit
GCCCTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG1100.5716067345666181No Hit
GCCCTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.4676782373726876No Hit
GCCCTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT760.3949282893369362No Hit
GCCCTCGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATG620.3221783413011848No Hit
GCCCTCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.3065890667220952No Hit
GCCCTCGGGAGCCCTGAGTTTGATTGTTTCCTGCTGTCTACTTCTCTTGGGTGTATTTGCTTCT580.30139264186239867No Hit
GCCCTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG540.28060694242361256No Hit
GCCCTCGGGGCCCACCTGTTTTTCCCTAGATTAGGTCTTGTTGTATTGCCAAGGGTGATCTTGT490.25462481812512994No Hit
GCCCTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.2338391186863438No Hit
GCCCTCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA390.20266056952816464No Hit
GCCCTCGGGGCCTTATTATGTGCTTAAGATACAATAGATTAGAATTTTATCTAAATATCTAGAA380.1974641446684681No Hit
GCCCTCGGGTCTGAAATGCGGTAGGTGAGGGAAAGAGCAGTTTCCGCAAACTCTGAACCCTGCC380.1974641446684681No Hit
GCCCTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC370.19226771980877158No Hit
GCCCTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.1818748700893785No Hit
GCCCTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG310.1610891706505924No Hit
GCCCTCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.1610891706505924No Hit
GCCCTCGGGGCTTCCTGTGTGCTACGGGATGGAACCCAGAGCCTCACACATCCTAAGCAACAAT310.1610891706505924No Hit
GCCCTCGGGAACTTAGTGATGAAAATCCAAACCAGAGAACATTAAGTGTGGTTATTCACAATCT280.1454998960715028No Hit
GCCCTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC280.1454998960715028No Hit
GCCCTCGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGGTCGAGC270.14030347121180628No Hit
GCCCTCGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCAGCCGGA260.13510704635210974No Hit
GCCCTCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA240.1247141966327167No Hit
GCCCTCGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATGC240.1247141966327167No Hit
GCCCTCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCATCAAGC220.11432134691332363No Hit
GCCCTCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.10392849719393059No Hit
GCCCTCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTCTACTG200.10392849719393059No Hit
GCCCTCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200.10392849719393059No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGAA201.8855077E-458.45789758
ATCTAGA201.8855077E-458.45789757
TATCTAG201.8855077E-458.45789756
ACCCAGA150.00452221358.30446255
TACCCAG150.00452221358.30446254
GGGCCGC150.00452221358.30446254
GTTGATT201.9102947E-458.3044654
AATATCT201.9102947E-458.3044654
ATATCTA201.9102947E-458.3044655
ATGGGCC150.00456957458.15183352
TGTTGAT201.9353401E-458.15183353
TTGTTGA201.9353401E-458.15183352
AAACCAC150.00456957458.15183352
CACATGT150.00456957458.15183328
TAAATAT201.9353401E-458.15183352
AAATATC201.9353401E-458.15183353
AACCACC150.00456957458.15183353
CAGGAAT150.00456957458.15183328
TAGGATT150.004617303658.00000434
ACGCTAC150.004617303658.00000441
Produced by FastQC (version 0.11.5)