##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526378_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19244
Sequences flagged as poor quality	0
Sequence length	64
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.01163999168572	39.0	38.0	39.0	35.0	40.0
2	37.68083558511744	39.0	38.0	39.0	35.0	40.0
3	37.938993972147166	39.0	38.0	39.0	35.0	40.0
4	37.90641238827686	39.0	38.0	39.0	35.0	40.0
5	37.26018499272501	39.0	37.0	39.0	33.0	40.0
6	37.91768863022241	39.0	38.0	39.0	35.0	40.0
7	37.91368738308044	39.0	38.0	39.0	35.0	40.0
8	37.8766888380794	39.0	38.0	39.0	35.0	40.0
9	38.07462066098524	39.0	38.0	39.0	35.0	40.0
10	37.21622323841197	39.0	37.0	39.0	33.0	40.0
11	36.92756183745583	39.0	36.0	39.0	33.0	40.0
12	36.71430056121388	38.0	36.0	39.0	31.0	40.0
13	36.539129079193515	38.0	35.0	39.0	31.0	40.0
14	36.60216171274163	38.0	36.0	39.0	31.0	40.0
15	36.51948659322386	38.0	36.0	39.0	31.0	40.0
16	36.24631053834962	38.0	35.0	39.0	30.0	40.0
17	36.030814799418	38.0	35.0	39.0	30.0	40.0
18	36.233111619205985	38.0	35.0	39.0	31.0	40.0
19	35.93603200997713	38.0	35.0	39.0	30.0	40.0
20	36.08579297443359	38.0	35.0	39.0	30.0	40.0
21	36.0068073165662	38.0	35.0	39.0	30.0	40.0
22	35.921533984618584	38.0	35.0	39.0	30.0	40.0
23	35.685512367491164	38.0	35.0	39.0	29.0	40.0
24	35.50561213884847	38.0	35.0	39.0	29.0	40.0
25	35.48040947827894	38.0	35.0	39.0	29.0	40.0
26	35.81869673664519	38.0	35.0	39.0	29.0	40.0
27	35.38344419039701	38.0	35.0	39.0	28.0	40.0
28	35.21170234878404	38.0	35.0	39.0	27.0	40.0
29	35.19034504261069	38.0	35.0	39.0	27.0	40.0
30	35.120609020993555	38.0	35.0	39.0	27.0	40.0
31	34.93374558303887	38.0	35.0	39.0	27.0	40.0
32	34.85741010184993	38.0	35.0	39.0	26.0	40.0
33	34.94211182706298	38.0	35.0	39.0	27.0	40.0
34	34.255248389108296	38.0	34.0	39.0	23.0	40.0
35	34.294117647058826	38.0	33.0	39.0	24.0	40.0
36	34.0513926418624	38.0	34.0	39.0	23.0	40.0
37	33.843275826231555	38.0	33.0	39.0	22.0	40.0
38	33.80679692371648	38.0	33.0	39.0	22.0	40.0
39	33.41633755975889	38.0	33.0	39.0	18.0	40.0
40	33.50732695905217	38.0	33.0	39.0	20.0	40.0
41	33.36442527541052	38.0	33.0	39.0	18.0	40.0
42	33.03564747453752	38.0	33.0	39.0	14.0	40.0
43	32.94044897110788	38.0	32.0	39.0	16.0	40.0
44	32.870141342756185	38.0	32.0	39.0	15.0	40.0
45	32.65313864061525	37.0	32.0	39.0	13.0	40.0
46	32.305289960507174	37.0	32.0	39.0	4.0	40.0
47	32.14856578673872	37.0	32.0	39.0	4.0	40.0
48	32.147890251506965	37.0	32.0	39.0	4.0	40.0
49	31.84519850342964	37.0	31.0	39.0	4.0	40.0
50	31.482124298482645	36.0	31.0	39.0	4.0	40.0
51	31.22874662232384	36.0	31.0	39.0	4.0	40.0
52	30.93816254416961	36.0	30.0	39.0	4.0	40.0
53	30.699386821866558	36.0	29.0	39.0	4.0	40.0
54	30.2928185408439	35.0	29.0	39.0	4.0	40.0
55	29.903710247349824	35.0	28.0	39.0	4.0	40.0
56	29.110839742257326	35.0	26.0	39.0	4.0	40.0
57	28.77208480565371	35.0	25.0	39.0	4.0	40.0
58	28.356630638120972	35.0	23.0	39.0	4.0	39.0
59	27.92345666181667	35.0	19.0	39.0	4.0	39.0
60	27.34353564747454	35.0	10.0	39.0	4.0	39.0
61	26.953128247765537	35.0	4.0	39.0	4.0	39.0
62	26.754520889627937	35.0	4.0	39.0	4.0	39.0
63	26.335741010184993	34.0	4.0	39.0	4.0	39.0
64	26.188370401164	34.0	4.0	39.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2	1	0.9205977031341988
2	2	1.3454166873850184
2	3	1.5876817031298316
2	4	1.7526077657726091
2	5	1.1090083669487427
2	6	1.56175901621247
2	7	1.3709418566785345
2	8	1.6999558388242235
2	9	1.305567045150866
2	10	1.5638763977048953
2	11	0.9166119892135995
2	12	1.7243801167652109
2	13	0.9354201611207245
2	14	0.39425146778781794
2	15	2.340809461032528
2	16	0.6827194753814183
2	17	2.471136362777216
2	18	1.3812096281007555
2	19	0.534020814919046
2	20	2.6449114202240054
2	21	1.9955616398836966
2	22	0.874453521503419
2	23	1.638965659853561
2	24	1.4440553377423342
2	25	1.3720231500099729
2	26	0.7697949382874398
2	27	1.6234044413640802
2	28	2.510899722992889
2	29	-0.0758740383819756
2	30	0.613911410095028
2	31	0.977742231476249
2	32	0.6389249610244789
2	33	0.6041377603327049
2	34	1.409645009427706
2	35	0.6966999583436078
2	36	1.50277927854534
2	37	1.3358166638282825
2	38	1.605900331861875
2	39	-5.6375540740766645
2	40	1.4966131933008882
2	41	2.247736241168205
2	42	1.8930264808093042
2	43	2.225896936395472
2	44	2.657835689150673
2	45	2.3180079825163915
2	46	4.1358753435974265
2	47	4.283723974727788
2	48	3.793306722899082
2	49	2.053581141962301
2	50	4.700362491502258
2	51	2.851125916934233
2	52	2.474933654846531
2	53	2.726632920720803
2	54	3.910956600306246
2	55	3.80563973585328
2	56	3.627231123599312
2	57	3.0565553773212315
2	58	4.05489074420267
2	59	3.7519102961668658
2	60	5.280242779199874
2	61	2.4848911720587523
2	62	2.2233263949892894
2	63	2.9326468993770725
2	64	1.0818961585810847
3	1	0.5707525018958108
3	2	0.6581101858370317
3	3	-0.33801489129741213
3	4	-0.061633720295503736
3	5	-0.3306201160233968
3	6	-0.9305010457070395
3	7	-0.8519683600397343
3	8	0.09004871808118509
3	9	-0.08762180933829455
3	10	-0.06460657443132334
3	11	0.47388753100926806
3	12	0.7986835223379671
3	13	-1.0490999627182802
3	14	1.0970378454968
3	15	0.582295529143984
3	16	0.314298422749836
3	17	-0.6310308198853178
3	18	-0.41136003134197097
3	19	-0.1532856866289407
3	20	0.21457086294846306
3	21	0.428998172391438
3	22	0.8992213233610045
3	23	-0.766607095564396
3	24	-0.8531582845486838
3	25	1.9231067413412433
3	26	1.9184017494329524
3	27	2.261175339196903
3	28	0.6533145836740033
3	29	2.2337234848378387
3	30	1.796573948794716
3	31	2.8786710240459144
3	32	0.7008444656684389
3	33	1.904447357855922
3	34	0.7223385078797193
3	35	2.3871024351237935
3	36	-1.797530318977877
3	37	-1.6115517572243476
3	38	-0.5179386774260522
3	39	-16.01526305240484
3	40	-1.2340369614978712
3	41	-1.1052049353023818
3	42	-1.33297971114116
3	43	-2.4459296889915265
3	44	-1.1502138464530454
3	45	-2.3414347419418142
3	46	2.631231380749128
3	47	3.5809375970188064
3	48	2.2731828838897954
3	49	1.9080703060489874
3	50	1.892312955898543
3	51	2.0090206537763358
3	52	1.6947478963325935
3	53	2.308676264374057
3	54	-0.6803746690435979
3	55	2.0657016553579233
3	56	0.7882218356736175
3	57	1.0998990305720042
3	58	0.13538610023982045
3	59	-0.19236214965356524
3	60	0.5650725005620991
3	61	0.6768416364550376
3	62	0.786793887249349
3	63	0.8985911718228934
3	64	-0.36082829962325036
4	1	0.1496998703168657
4	2	-0.499784551005078
4	3	0.8198798455446905
4	4	1.1488925954939688
4	5	0.774643041871343
4	6	0.9116042174508578
4	7	0.35855795574974536
4	8	1.090048718081185
4	9	1.122904506451178
4	10	1.3038144782002519
4	11	0.10546647837768575
4	12	0.7460519433905972
4	13	0.84563687938698
4	14	0.2549325823389026
4	15	1.1612428975650388
4	16	1.5774563174866785
4	17	1.0005481274830998
4	18	1.4833768107632892
4	19	1.1625037870552646
4	20	-0.6275344002094272
4	21	0.6921560671282805
4	22	0.16237921809784694
4	23	-1.1876597271433411
4	24	0.9363153996618436
4	25	0.554685688709661
4	26	0.6552438546961099
4	27	0.8401227076179509
4	28	1.0743672152529484
4	29	-0.13469756779374364
4	30	-0.3086892091000166
4	31	-2.437118449638298
4	32	-1.299155534331561
4	33	-2.569236852670393
4	34	-0.9092404394887055
4	35	-8.244476512244624
4	36	-0.7975303189778771
4	37	-0.6115517572243476
4	38	-1.9916228879523707
4	39	-2.4889472629311555
4	40	-2.54982643518208
4	41	-3.8946786195129093
4	42	-3.9645586585095813
4	43	-2.972245478465208
4	44	-2.7817927938214666
4	45	-2.551961057731287
4	46	-5.000347566619293
4	47	-4.840115034560139
4	48	-5.20050132663652
4	49	-4.460350746582591
4	50	-4.423476517785669
4	51	-5.990979346223661
4	52	-6.621041577351615
4	53	-5.743955314573313
4	54	-5.364585195359389
4	55	-5.407982555168395
4	56	-4.317041322221119
4	57	-3.7422062325858896
4	58	-3.075140215549652
4	59	-2.9292042549167228
4	60	-3.5928222362800035
4	61	-3.691579416176541
4	62	-4.160574533803281
4	63	-3.575093038703425
4	64	-3.729249352254829
5	1	-0.12515393085272564
5	2	1.5382271449013558
5	3	0.9602307227376699
5	4	1.1284247592366654
5	5	1.04364888982456
5	6	0.94084398353273
5	7	1.1421836867438913
5	8	1.0725048584320618
5	9	1.070272927503808
5	10	0.7599548290774436
5	11	-1.067048141505353
5	12	-1.6252930858491723
5	13	-1.195298793127641
5	14	-0.6515001661991064
5	15	-0.42062844746420325
5	16	-20.81728052461858
5	17	-2.6661185391835645
5	18	-2.925979914382907
5	19	-2.939835394231281
5	20	-2.721101651671418
5	21	-0.3867913012927744
5	22	-1.0013634719606372
5	23	-0.49175329439480464
5	24	-1.1572518518001473
5	25	-0.22601606567630483
5	26	2.292670755280909
5	27	2.126672415220291
5	28	1.6971742327968116
5	29	1.5875246544284778
5	30	2.904761083297643
5	31	-0.963434239111983
5	32	-1.0506175226356547
5	33	-0.6540321743078223
5	34	-0.34198897750039947
5	35	-5.522254290022403
5	36	-0.1834952312585827
5	37	-1.5530722250606033
5	38	-4.23723692304009
5	39	-2.170233812638756
5	40	-2.5556743883984545
5	41	-2.994093824191271
5	42	-19.587365676053444
5	43	-4.712011560336556
5	44	-2.518634899084624
5	45	-3.0022534553921076
5	46	-2.7693534145725103
5	47	-1.9483021690630657
5	48	-3.4256475254669283
5	49	-3.439882910325281
5	50	-2.4761080967330393
5	51	-1.982207416399099
5	52	-1.2146088288136028
5	53	-2.8550664256844236
5	54	-1.76224601407284
5	55	-3.4459942510748256
5	56	-3.065579333917025
5	57	-2.531679916796417
5	58	-2.5137367067777205
5	59	-1.829789050238361
5	60	-2.7360970900811736
5	61	-2.691579416176541
5	62	-2.9237324285401236
5	63	-2.0019936234987448
5	64	-1.2350973054712036
6	1	-1.2558728851010912
6	2	-2.1251715479090976
6	3	-2.7064359439289944
6	4	-2.306215763639152
6	5	-1.3615798683453804
6	6	-1.7911821602581242
6	7	-1.6878816727332264
6	8	-1.2412206317640155
6	9	-1.929727072496192
6	10	-0.9655353670009887
6	11	-0.20103506960992945
6	12	-0.3344434126465572
6	13	-1.0645798388792755
6	14	-2.488101473388653
6	15	-1.718014068379233
6	16	-2.7878687599126977
6	17	-1.7053342254580741
6	18	-1.2658491954286575
6	19	-2.583626243904483
6	20	-2.1197944621289366
6	21	-0.8867913012927744
6	22	-1.890252360849523
6	23	-1.184563751911142
6	24	-1.2618270151988398
6	25	-0.2162121441076721
6	26	-0.46549917935961815
6	27	-0.37659555863591976
6	28	-0.6067473358306401
6	29	-0.7817563913231567
6	30	-0.327265060493211
6	31	-1.198728356759041
6	32	-0.4198985683872891
6	33	-0.27821518084376606
6	34	-0.88447263763112
6	35	-1.4797706298916822
6	36	-3.320750133219363
6	37	-3.7818303949952465
6	38	-2.80586437402048
6	39	-21.04931877995902
6	40	-7.091622100816757
6	41	-4.869910817655324
6	42	-3.9893264603671668
6	43	-5.127044240075115
6	44	-4.106870193202273
6	45	-4.2114037821894925
6	46	-1.0405952446378706
6	47	-1.30451131938986
6	48	-1.2066932771009142
6	49	-1.299360034508286
6	50	-0.41728456732127484
6	51	-0.7371093771834154
6	52	-0.054478109859353196
6	53	-2.4498376675144904
6	54	-1.0302198702819894
6	55	-3.3120073229702527
6	56	-0.31394534698891974
6	57	0.350673024380054
6	58	-1.2980504322679174
6	59	-1.4245602920684277
6	60	0.1625957203763413
6	61	0.07312646617640084
6	62	0.9292087479304634
6	63	1.462058664082953
6	64	-0.03575090024244432
7	1	0.4720682913694958
7	2	1.5035049226791344
7	3	1.1060640560710056
7	4	1.3298136481255511
7	5	-0.19246222128655432
7	6	0.8227884279771729
7	7	0.8157947978550055
7	8	1.0308381917653975
7	9	0.8202729275038081
7	10	-0.2192118375892207
7	11	1.4607296362724256
7	12	1.0552624697063848
7	13	1.3949789846501375
7	14	0.133222056023115
7	15	1.1349271080913539
7	16	1.1202194753814183
7	17	1.0005481274830998
7	18	-0.8357021366051285
7	19	0.31710905021316194
7	20	1.3691761261063604
7	21	1.9882086987072256
7	22	0.797247639150477
7	23	1.098524483382974
7	24	1.1205259259776312
7	25	1.3573172676570309
7	26	-0.44344035583020514
7	27	0.5572279707758483
7	28	0.6763408994634759
7	29	0.6778024322062564
7	30	0.724205527742086
7	31	-5.650934239111983
7	32	-3.960339744857876
7	33	-4.167921063196708
7	34	-23.744766755278178
7	35	-8.306976512244624
7	36	-6.475161897925247
7	37	-5.5391833361717175
7	38	-6.008070256373422
7	39	-4.725789368194313
7	40	-5.882063277287344
7	41	3.769795064697618
7	42	4.245967657279891
7	43	3.781043995219001
7	44	3.731365100915376
7	45	4.3658021001634495
7	46	2.2167576965386004
7	47	1.5447533864924914
7	48	3.9771302523108503
7	49	2.85872820078583
7	50	4.523891903266961
7	51	4.351125916934233
7	52	4.375668948964176
7	53	4.506044685426687
7	54	4.605809541482717
7	55	0.9637279711473958
7	56	-0.40585711169480376
7	57	-1.031679916796417
7	58	-1.5554033734443884
7	59	-1.0936779391272502
7	60	-1.5138748678589522
7	61	-1.4415794161765412
7	62	-1.1737324285401236
7	63	-0.828382512387634
7	64	-3.498986194360093
8	1	-0.18070948640828277
8	2	0.9271160337902487
8	3	0.4046751671821198
8	4	0.8506469814588868
8	5	-0.28968444350877576
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118	22	1.5097476391504756
118	23	2.086024483382971
118	24	0.6205259259776312
118	25	0.4573172676570323
118	26	0.33155964416979344
118	27	0.5822279707758469
118	28	0.413840899463473
118	29	1.4653024322062578
118	30	0.9992055277420846
118	31	1.7365657608880198
118	32	1.1271602551421225
118	33	1.4070789368032948
118	34	1.4802332447218234
118	35	2.2055234877553787
118	36	3.649838102074753
118	37	1.9358166638282839
118	38	2.6294297436265808
118	39	3.4242106318056855
118	40	1.7054367227126548
118	41	1.4947950646976196
118	42	0.6959676572798941
118	43	1.2935439952190038
118	44	0.0813651009153773
118	45	-0.6466978998365498
118	46	1.0917576965386004
118	47	0.25725338649248997
118	48	0.8021302523108531
118	49	1.6212282007858327
118	50	-0.026108096733040043
118	51	1.8511259169342331
118	52	2.363168948964173
118	53	3.0560446854266843
118	54	2.8433095414827143
118	55	3.4762279711473987
118	56	4.406642888305196
118	57	4.668320083203586
118	58	2.6195966265556123
118	59	3.53132206087275
118	60	2.836125132141049
118	61	1.0584205838234588
118	62	2.476267571459875
118	63	2.7091174876123674
118	64	1.7760138056399057
119	1	0.25496302821159844
119	2	0.13179439636334678
119	3	0.6619851087025879
119	4	-0.43005477292707894
119	5	-0.12009380023392424
119	6	0.5958147437666454
119	7	0.30592637680237544
119	8	0.30057503387065765
119	9	0.17553608539854793
119	10	0.6196039518844643
119	11	-0.5261124689907319
119	12	-0.14868489871467006
119	13	-0.5754157521919652
119	14	-0.7976989966084673
119	15	-0.10191499717180363
119	16	-0.8962278930396366
119	17	-0.6310308198853178
119	18	-1.2534652944998683
119	19	0.2151353660026345
119	20	-0.3643765054725847
119	21	0.5342613302861707
119	22	1.057116060203107
119	23	1.233392904435604
119	24	-1.3794740740223688
119	25	0.23889621502544856
119	26	-1.2394929874091503
119	27	0.313806918144266
119	28	-0.5045801531681064
119	29	-0.8189080941095312
119	30	-0.2034260512052839
119	31	1.4576183924669621
119	32	0.01663393935265134
119	33	0.904447357855922
119	34	-0.06713517633080812
119	35	1.702891908808006
119	36	-1.6922671610831443
119	37	-0.08523596775066267
119	38	-0.20214920374183976
119	39	-1.278420947141683
119	40	0.2922788279758137
119	41	-0.15783651424975176
119	42	-2.6487691848253725
119	43	-1.182771794254684
119	44	-2.518634899084624
119	45	-2.183540005099708
119	46	-3.737189671882451
119	47	-2.8401150345601387
119	48	-4.884711852952307
119	49	-2.9866665360562763
119	50	-2.370844938838303
119	51	-1.1488740830657669
119	52	-1.778936314193718
119	53	-1.8492184724680492
119	54	-5.048795721675177
119	55	-5.986929923589447
119	56	-5.317041322221119
119	57	-5.7948378115332595
119	58	-5.233034952391758
119	59	-3.2976253075483015
119	60	-3.6454538152273734
119	61	-2.7968425740712775
119	62	-2.2658376916980174
119	63	-1.943514091335004
119	64	-1.3608282996232504
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	20.0
5	2.0
6	6.0
7	5.0
8	31.0
9	20.0
10	26.0
11	34.0
12	36.0
13	58.0
14	66.0
15	102.0
16	117.0
17	124.0
18	138.0
19	121.0
20	150.0
21	174.0
22	215.0
23	198.0
24	229.0
25	234.0
26	255.0
27	346.0
28	457.0
29	462.0
30	598.0
31	725.0
32	909.0
33	1163.0
34	1338.0
35	1593.0
36	1769.0
37	2495.0
38	3254.0
39	1769.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	0.0	100.0
3	0.0	0.0	0.0	100.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	98.52933534272202	0.6859637270695838	0.28062152471028423	0.5040794054981033
8	98.25856422519104	0.7641524146176639	0.6445911524666008	0.33269220772469715
9	97.22510912492204	1.3198919143629184	0.5819995842860112	0.8729993764290168
10	52.26564123882768	29.99376429016836	6.989191436291831	10.751403034712117
11	23.092912076491373	27.016212845562254	23.78403658283101	26.10683849511536
12	40.59966742880898	13.988775722303057	25.904177925587195	19.50737892330077
13	22.957805030139262	14.913739347329038	36.130742049469966	25.99771357306173
14	25.514446061109957	24.277696944502182	32.7790480149657	17.428808979422158
15	32.30841267363821	20.70131626866448	28.968315904479475	18.021955153217835
16	20.855642771741955	22.711441492956283	26.459427145604824	29.973488589696938
17	23.608654946426714	21.756995734942265	21.434515759908457	33.19983355872257
18	24.755768031594265	28.559551028892123	21.118270629806695	25.56641030970692
19	20.44793182290584	25.696320931199335	31.71897734358761	22.13676990230721
20	21.069868995633186	28.191931794551884	22.894572676232062	27.843626533582867
21	22.02244855539389	27.75410517563916	26.501766784452297	23.721679484514656
22	24.73498233215548	31.34483475368946	20.34400332571191	23.576179588443154
23	30.892745790895866	29.328621908127207	20.577842444398254	19.200789856578673
24	22.210674011328795	35.87798160370005	19.67468689913215	22.236657485839007
25	21.501741979096252	28.08486298164422	25.240497114034632	25.172897925224895
26	24.701112381744466	23.42759122569914	21.743424472398377	30.12787192015802
27	18.281697474615985	23.941681853683935	22.671179380369697	35.10544129133038
28	19.197334860236325	28.566966841913487	24.891988964655663	27.34370933319452
29	23.894035599042365	24.258353284063702	25.798896637868225	26.04871447902571
30	17.200270706439692	32.7294497371024	26.893643604560364	23.17663595189755
31	21.214646596176884	32.24126256575863	28.428563987707694	18.11552685035679
32	17.326809972414512	32.24379326497684	35.01795659189091	15.411440170717745
33	21.34006663890046	31.226572261557685	30.148896293211163	17.284464806330696
34	19.60988839052884	32.84656305413581	33.80098049441953	13.742568060915824
35	17.832404562262383	36.649132857663666	33.857611582730065	11.66085099734389
36	18.286518317786232	35.619365261347646	31.148053572359164	14.946062848506958
37	17.970908711746	32.91799176268182	35.62379437985507	13.487305145717116
38	16.551112964604076	34.858989730490535	34.98410050565605	13.605796799249337
39	16.9645656945751	32.20445280652242	36.42730218459287	14.403679314309606
40	17.2090112640801	33.06737588652482	37.234042553191486	12.489570296203588
41	17.04581028905353	31.895022435562975	37.51434832515913	13.544818950224355
42	16.595277893857084	32.422691182615964	36.78959465106561	14.192436272461345
43	16.648383221020737	32.1997597032858	36.943007888000835	14.208849187692628
44	16.74238863648232	30.388009817745054	36.236879210402634	16.632722335369994
45	17.12897664942799	32.89453063783106	35.66316669278587	14.313326019955074
46	18.571353921722316	32.96232429325391	35.1518001776663	13.314521607357474
47	15.482286550318738	32.51645940014631	38.09697983070331	13.904274218831643
48	19.33444781109602	32.452199352209796	35.39859993731063	12.814752899383555
49	17.335423197492165	33.85057471264368	35.54858934169279	13.265412748171368
50	15.155318481330404	33.83537286894676	35.01725760903671	15.99205104068612
51	15.629083686163817	33.24969944069834	35.66985520882338	15.451361664314463
52	14.111744107040192	34.83510165682329	38.11738880468301	12.935765431453511
53	15.980756157506667	32.97076818490823	36.01945301469435	15.02902264289076
54	16.71111111111111	34.07581699346405	34.614379084967325	14.598692810457518
55	16.740779492545123	34.43369081872875	34.28720899816898	14.538320690557155
56	16.055453832069055	34.86790478681663	32.09521318336385	16.98142819775046
57	15.51056706423938	34.15986608077004	32.83113622096673	17.498430634023855
58	14.857651245551601	34.995813271927986	33.19028679087293	16.956248691647478
59	16.173931243786303	34.26298990110407	33.03856417769871	16.524514677410913
60	15.726101978850382	34.07496597214951	33.01748508009633	17.181446968903778
61	15.87509153677163	34.78920389162046	31.614185584266135	17.72151898734177
62	17.234521379599098	33.90903857225101	31.43350604490501	17.422934003244883
63	17.3346588530766	34.30305567182922	29.525952281289243	18.83633319380494
64	17.813383600377	35.4382657869934	28.746465598491987	18.001885014137606
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.0
8	4.0
9	4.5
10	5.5
11	6.0
12	66.0
13	137.0
14	148.0
15	120.5
16	97.5
17	102.0
18	150.5
19	199.0
20	221.0
21	258.0
22	273.0
23	307.5
24	398.0
25	454.0
26	544.5
27	683.0
28	731.0
29	811.5
30	1063.5
31	1235.0
32	1018.0
33	801.0
34	835.5
35	653.5
36	437.0
37	403.0
38	390.5
39	412.0
40	478.5
41	455.5
42	366.0
43	354.5
44	343.0
45	335.5
46	311.0
47	294.0
48	287.0
49	339.5
50	399.0
51	385.0
52	372.0
53	373.0
54	415.0
55	468.5
56	480.0
57	543.5
58	607.0
59	658.5
60	682.5
61	655.0
62	716.0
63	747.5
64	718.0
65	669.5
66	628.5
67	636.0
68	753.0
69	870.0
70	657.5
71	343.0
72	241.0
73	191.5
74	114.0
75	86.0
76	69.0
77	37.5
78	23.0
79	18.0
80	12.0
81	11.0
82	9.5
83	8.0
84	8.0
85	5.0
86	2.0
87	1.5
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.005196424859696528
8	0.0363749740178757
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.11951777177302016
16	0.0363749740178757
17	0.09353564747453752
18	0.0
19	0.0
20	0.041571398877572226
21	0.0
22	0.0
23	0.0
24	0.005196424859696528
25	0.06755352317605487
26	0.031178549158179174
27	0.20266056952816464
28	0.17148202036998544
29	0.15589274579089588
30	0.1818748700893785
31	0.2338391186863438
32	0.1610891706505924
33	0.18707129494907504
34	0.363749740178757
35	0.22344626896695072
36	0.2858033672833091
37	0.32737476616088135
38	0.31698191644148827
39	0.5716067345666181
40	0.35335689045936397
41	0.40532113905632927
42	0.5196424859696529
43	0.5248389108293494
44	0.4936603616711702
45	0.5248389108293494
46	0.5560174599875286
47	0.550821035127832
48	0.5300353356890459
49	0.540428185408439
50	0.6339638328829765
51	0.5871960091457078
52	0.5768031594263148
53	0.6287674080232799
54	0.6183745583038869
55	0.6703388069008522
56	0.6703388069008522
57	0.6651423820411556
58	0.7067137809187279
59	0.6911245063396384
60	0.7378923300769071
61	0.6547495323217627
62	0.7119102057784245
63	0.6859280814799418
64	0.7586780295156932
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
64	19244.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.063188526293914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.09931069696196	50.74308875493661
2	8.433324823419284	10.29931407191852
3	2.9444302612543614	5.393889004364997
4	1.8126116926219042	4.427353980461443
5	0.9446004595353586	2.8840157971315734
6	0.5871840694408986	2.151319891914363
7	0.4850651008424815	2.073373519018915
8	0.33188664794485573	1.621284556225317
9	0.2808271636456472	1.543338183329869
>10	0.970130201684963	9.592600290999792
>50	0.051059484299208584	2.073373519018915
>100	0.059569398349076666	7.1970484306796925
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCCCTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	441	2.2916233631261695	No Hit
GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	268	1.3926418623986696	No Hit
GCCCTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC	215	1.1172313448347537	No Hit
GCCCTCGGGGCGATACTGCTAATTACACACATGTGATGTTTTTGGGTTTGTTTGTTGATTTTTT	129	0.6703388069008522	No Hit
GCCCTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	111	0.5768031594263148	No Hit
GCCCTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	111	0.5768031594263148	No Hit
GCCCTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCG	110	0.5716067345666181	No Hit
GCCCTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	90	0.4676782373726876	No Hit
GCCCTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	76	0.3949282893369362	No Hit
GCCCTCGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCCGTCATG	62	0.3221783413011848	No Hit
GCCCTCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	59	0.3065890667220952	No Hit
GCCCTCGGGAGCCCTGAGTTTGATTGTTTCCTGCTGTCTACTTCTCTTGGGTGTATTTGCTTCT	58	0.30139264186239867	No Hit
GCCCTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGAGGCAGGGG	54	0.28060694242361256	No Hit
GCCCTCGGGGCCCACCTGTTTTTCCCTAGATTAGGTCTTGTTGTATTGCCAAGGGTGATCTTGT	49	0.25462481812512994	No Hit
GCCCTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	45	0.2338391186863438	No Hit
GCCCTCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGA	39	0.20266056952816464	No Hit
GCCCTCGGGGCCTTATTATGTGCTTAAGATACAATAGATTAGAATTTTATCTAAATATCTAGAA	38	0.1974641446684681	No Hit
GCCCTCGGGTCTGAAATGCGGTAGGTGAGGGAAAGAGCAGTTTCCGCAAACTCTGAACCCTGCC	38	0.1974641446684681	No Hit
GCCCTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC	37	0.19226771980877158	No Hit
GCCCTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	35	0.1818748700893785	No Hit
GCCCTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGAGCGAAGTG	31	0.1610891706505924	No Hit
GCCCTCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	31	0.1610891706505924	No Hit
GCCCTCGGGGCTTCCTGTGTGCTACGGGATGGAACCCAGAGCCTCACACATCCTAAGCAACAAT	31	0.1610891706505924	No Hit
GCCCTCGGGAACTTAGTGATGAAAATCCAAACCAGAGAACATTAAGTGTGGTTATTCACAATCT	28	0.1454998960715028	No Hit
GCCCTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC	28	0.1454998960715028	No Hit
GCCCTCGGGGCTCTTCGCCATCTTTACCCCGGCAGGCCGACATCTATCACCATGAAGGTCGAGC	27	0.14030347121180628	No Hit
GCCCTCGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCCCAGCCGGA	26	0.13510704635210974	No Hit
GCCCTCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA	24	0.1247141966327167	No Hit
GCCCTCGGGAACATTTCCTGGGCCTTTCAGGAATACCACGACGCTACTCAGACTACCCAGATGC	24	0.1247141966327167	No Hit
GCCCTCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCCCATCAAGC	22	0.11432134691332363	No Hit
GCCCTCGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	20	0.10392849719393059	No Hit
GCCCTCGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCAGCTCTACTG	20	0.10392849719393059	No Hit
GCCCTCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20	0.10392849719393059	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.005196424859696529	0.0	0.0	0.0	0.0
9	0.005196424859696529	0.0	0.0	0.0	0.0
10	0.015589274579089585	0.0	0.0	0.0	0.0
11	0.015589274579089585	0.0	0.0	0.0	0.0
12	0.015589274579089585	0.0	0.0	0.0	0.0
13	0.015589274579089585	0.0	0.0	0.0	0.0
14	0.0363749740178757	0.0	0.0	0.0	0.0
15	0.04157139887757223	0.0	0.0	0.0	0.0
16	0.05196424859696529	0.0	0.0	0.0	0.0
17	0.06755352317605487	0.0	0.0	0.0	0.0
18	0.06755352317605487	0.0	0.0	0.0	0.0
19	0.0727499480357514	0.0	0.0	0.0	0.0
20	0.09353564747453752	0.0	0.0	0.0	0.0
21	0.09353564747453752	0.0	0.0	0.0	0.0
22	0.15589274579089588	0.0	0.0	0.0	0.0
23	0.17667844522968199	0.0	0.0	0.0	0.0
24	0.18707129494907504	0.0	0.0	0.0	0.0
25	0.1974641446684681	0.0	0.0	0.0	0.0
26	0.20785699438786115	0.0	0.0	0.0	0.0
27	0.2182498441072542	0.0	0.0	0.0	0.0
28	0.22864269382664726	0.0	0.0	0.0	0.0
29	0.2390355435460403	0.0	0.0	0.0	0.0
30	0.29619621700270216	0.0	0.0	0.0	0.0
31	0.29619621700270216	0.0	0.0	0.0	0.0
32	0.30139264186239867	0.0	0.0	0.0	0.0
33	0.31178549158179175	0.0	0.0	0.0	0.0
34	0.3429640407399709	0.0	0.0	0.0	0.0
35	0.4209104136354188	0.0	0.0	0.0	0.0
36	0.4261068384951154	0.0	0.0	0.0	0.0
37	0.4416961130742049	0.0	0.0	0.0	0.0
38	0.452088962793598	0.0	0.0	0.0	0.0
39	0.4676782373726876	0.0	0.0	0.0	0.0
40	0.49885678653086674	0.0	0.0	0.0	0.0
41	0.5092496362502599	0.0	0.0	0.0	0.0
42	0.5248389108293494	0.0	0.0	0.0	0.0
43	0.5456246102681355	0.0	0.0	0.0	0.0
44	0.5664103097069216	0.0	0.0	0.0	0.0
45	0.6079817085844939	0.0	0.0	0.0	0.0
46	0.62876740802328	0.0	0.0	0.0	0.0
47	0.6443566826023696	0.0	0.0	0.0	0.0
48	0.6807316566202453	0.0	0.0	0.0	0.0
49	0.6963209311993348	0.0	0.0	0.0	0.0
50	0.7015173560590314	0.0	0.0	0.0	0.0
51	0.7119102057784245	0.0	0.0	0.0	0.0
52	0.717106630638121	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGAA	20	1.8855077E-4	58.457897	58
ATCTAGA	20	1.8855077E-4	58.457897	57
TATCTAG	20	1.8855077E-4	58.457897	56
ACCCAGA	15	0.004522213	58.304462	55
TACCCAG	15	0.004522213	58.304462	54
GGGCCGC	15	0.004522213	58.304462	54
GTTGATT	20	1.9102947E-4	58.30446	54
AATATCT	20	1.9102947E-4	58.30446	54
ATATCTA	20	1.9102947E-4	58.30446	55
ATGGGCC	15	0.004569574	58.151833	52
TGTTGAT	20	1.9353401E-4	58.151833	53
TTGTTGA	20	1.9353401E-4	58.151833	52
AAACCAC	15	0.004569574	58.151833	52
CACATGT	15	0.004569574	58.151833	28
TAAATAT	20	1.9353401E-4	58.151833	52
AAATATC	20	1.9353401E-4	58.151833	53
AACCACC	15	0.004569574	58.151833	53
CAGGAAT	15	0.004569574	58.151833	28
TAGGATT	15	0.0046173036	58.000004	34
ACGCTAC	15	0.0046173036	58.000004	41
>>END_MODULE