FastQCFastQC Report
Fri 17 Jun 2016
SRR1526377_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526377_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18780
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA6553.487752928647497No Hit
GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT4232.252396166134185No Hit
GCCCTCGGGGCGATACTGCTAATTACACACATGTGATGTTTTTGGGTTTGTTTGTT2101.1182108626198082No Hit
GCCCTCGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC1881.0010649627263046No Hit
GCCCTCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA1520.8093716719914802No Hit
GCCCTCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1180.6283280085197018No Hit
GCCCTCGGGGCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCC1070.56975505857295No Hit
GCCCTCGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT770.4100106496272631No Hit
GCCCTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.3727369542066028No Hit
GCCCTCGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGAC690.36741214057507987No Hit
GCCCTCGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.36741214057507987No Hit
GCCCTCGGGTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCC640.3407880724174654No Hit
GCCCTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG570.3035143769968051No Hit
GCCCTCGGGTCTGAAATGCGGTAGGTGAGGGAAAGAGCAGTTTCCGCAAACTCTGA550.2928647497337593No Hit
GCCCTCGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCGCCATGAGA530.28221512247071356No Hit
GCCCTCGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.27689030883919064No Hit
GCCCTCGGGACAGTGAATCCTAGCACATGGAATGGGTTGGAGGGAAGAGAATGAGA480.25559105431309903No Hit
GCCCTCGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.25559105431309903No Hit
GCCCTCGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT470.25026624068157616No Hit
GCCCTCGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAGA460.24494142705005326No Hit
GCCCTCGGGAGCCCTGAGTTTGATTGTTTCCTGCTGTCTACTTCTCTTGGGTGTAT450.23961661341853036No Hit
GCCCTCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.22896698615548455No Hit
GCCCTCGGGGCTTCCTGTGTGCTACGGGATGGAACCCAGAGCCTCACACATCCTAA380.20234291799787005No Hit
GCCCTCGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAG370.19701810436634717No Hit
GCCCTCGGGGAGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACA360.19169329073482427No Hit
GCCCTCGGGGCCTTATTATGTGCTTAAGATACAATAGATTAGAATTTTATCTAAAT360.19169329073482427No Hit
GCCCTCGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.1863684771033014No Hit
GCCCTCGGGGCCCACCTGTTTTTCCCTAGATTAGGTCTTGTTGTATTGCCAAGGGT330.1757188498402556No Hit
GCCCTCGGGGGTCGAGACTCCTCTACCCTGTGCAAAGGTGTATGAGTTTCGACCCC320.1703940362087327No Hit
GCCCTCGGGAACTTAGTGATGAAAATCCAAACCAGAGAACATTAAGTGTGGTTATT320.1703940362087327No Hit
GCCCTCGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA300.1597444089456869No Hit
GCCCTCGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC290.154419595314164No Hit
GCCCTCGGGGCTCCCCGCGCACTGCCTGGTGTGGTTTGGGCCACGCATAAAAGGTT280.1490947816826411No Hit
GCCCTCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA280.1490947816826411No Hit
GCCCTCGGGGCCTTTCCGCTCGGCCGTTCTCCTGTATAGGAGGCAGCCATGGCGCC270.14376996805111822No Hit
GCCCTCGTGTGCGGTGTTGTCTGTGAATGCTAAATCTGCCATCCCTTCCAGCCCAC260.13844515441959532No Hit
GCCCTCGGGGCTTTCTGACCCTTTCCGGGCGTTCAAGATGTCGAAGCGAGGACGCG260.13844515441959532No Hit
GCCCTCGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA250.13312034078807242No Hit
GCCCTCGGGATGGCACTGGCCCCAACATTCTCACCGACATCACCAAGGGTGTGCAG230.12247071352502663No Hit
GCCCTCGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGG230.12247071352502663No Hit
GCCCTCGGGCCATCTCATCCCTGAGCCATGATCAAACTCTACGTACTGAGTCGGAG230.12247071352502663No Hit
GCCCTCGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC230.12247071352502663No Hit
GCCCTCGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA230.12247071352502663No Hit
GCCCTCGGGGTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGC220.11714589989350374No Hit
GCCCTCGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA220.11714589989350374No Hit
GCCCTCGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.11182108626198084No Hit
GCCCTCGGGAAGCAGTGGTATCAACGCAGAGTCGAAAAAAAAAAAAAAAAAAAAAA210.11182108626198084No Hit
GCCCTCGGGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT210.11182108626198084No Hit
GCCCTCGGGGCTTTCGGCCGCCGCAGCCATCTTCCAGTAACTCGCCAAAATGACGA210.11182108626198084No Hit
GCCCTCGGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTGCAGCC210.11182108626198084No Hit
GCCCTCGGGGCCCACTAGTCTTCTGAGATCTTGGGCCTGTTGCTAGCCACGTGGGA210.11182108626198084No Hit
GCCCTCGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.11182108626198084No Hit
GCCCTCGGGGGTTTTAAAGTAAATTACCACTACATGTCTCAGGTGAAAAATGCCAA200.10649627263045794No Hit
GCCCTCGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAG200.10649627263045794No Hit
GCCCTCGGGGTGTGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.10649627263045794No Hit
GCCCTCGGGCTACGAATCAAAATTTAACCCAAAAAAAAAAAAAAAAAAAAAAAAAA200.10649627263045794No Hit
GCCCTCGGGCCATCTCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAG200.10649627263045794No Hit
GCCCTCGGGGTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT200.10649627263045794No Hit
GCCCTCGGGATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.10649627263045794No Hit
GCCCTCCACCATATCACACATTCGAGGAACCAACCTATGTAAAAGTAAAATAAGAA200.10649627263045794No Hit
GCCCTCGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTT190.10117145899893502No Hit
GCCCTCGGGATCCATATCATACTCTATCCCAAAAAAAACTTCGTTTCCAATACTTA190.10117145899893502No Hit
GCCCTCGGGGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190.10117145899893502No Hit
GCCCTCGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGGG190.10117145899893502No Hit
GCCCTCGGGGCTTTTTTTTTTTTCCTGTGGGAGCAGCCGGGGAGAGAGGGGCGGGG190.10117145899893502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGTTT308.940315E-750.7602235
ATGTTTT308.940315E-750.7602236
TCGTAGG150.00781961350.7602234
TGATGTT308.940315E-750.7602234
GTGATGT309.284686E-750.48509633
GTTTTTG309.461073E-750.3486538
ATGTGAT309.461073E-750.3486531
TGTGATG309.461073E-750.3486532
TTGTTTG251.9272838E-550.34864848
TGTTTGT251.9272838E-550.34864849
TTTGTTT251.9272838E-550.34864847
GGGTTTG309.640298E-750.2129444
CACGCCG150.00816338650.2129444
CCACGCC150.00816338650.2129443
TGGGTTT309.640298E-750.2129443
TTTGGGT309.640298E-750.2129441
CACATGT301.0007461E-649.94370328
ACATGTG301.0007461E-649.94370329
CATGTGA301.0007461E-649.94370330
GCCTCGG150.00842849949.8101625
Produced by FastQC (version 0.11.5)