FastQCFastQC Report
Fri 17 Jun 2016
SRR1526376_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526376_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41545
Sequences flagged as poor quality0
Sequence length64
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG6241.5019857985317127No Hit
AAGCGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC4861.1698158623179684No Hit
AAGCGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1980.4765916476110242No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1890.45492839090143217No Hit
AAGCGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1870.45011433385485616No Hit
AAGCGGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA1690.40678782043567213No Hit
AAGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGCG1590.38271753520279217No Hit
AAGCGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA1490.35864724996991215No Hit
AAGCGGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCC1250.3008785654110001No Hit
AAGCGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1200.2888434227945601No Hit
AAGCGGGGGGCAGCCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACT1100.2647731375616801No Hit
AAGCGGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT1010.2431098808520881No Hit
AAGCGGGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCT990.2382958238055121No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT870.20941148152605607No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC850.20459742447948007No Hit
AAGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG830.19978336743290406No Hit
AAGCGGGGCAAGGGACACCGAAAAGCAGTGTCTTATGCCAAGTTTGTAAGTGGTGAAGAAATTG790.19015525333975208No Hit
AAGCGGGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACA700.16849199663016007No Hit
AAGCGGGGGAAGATGAAGAGTTAAGTGACCTTAATTAACCTGTTCTGTGCCCCATCCTAAGGCA690.16608496810687207No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT690.16608496810687207No Hit
AAGCGGGGGGAGGCTCTCCGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGC680.16367793958358406No Hit
AAGCGGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC670.16127091106029606No Hit
AAGCGGGGGAGCTTTTGGAAAGCTCTTTTTTACATAATAAAAAATATACAGAAAACAGTGCTTC650.15645685401372006No Hit
AAGCGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTCCTCTCCCC630.15164279696714406No Hit
AAGCGGGGGCAGTCTGCTGCTGGAACACATACATCGATCTTTATTCTCCACTGATTGGTTGCCC620.14923576844385605No Hit
AAGCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCC610.14682873992056805No Hit
AAGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG600.14442171139728005No Hit
AAGCGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCTC580.13960765435070405No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGCAAGGCCCC560.13479359730412804No Hit
AAGCGGGGGGACTAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAA560.13479359730412804No Hit
AAGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCCC550.13238656878084004No Hit
AAGCGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC530.12757251173426404No Hit
AAGCGGGGGGTCTCTCGGGTGGAGTCTTCTGACTGCTGGTGGAGCAGGTCTCAGGAATCTCTTC530.12757251173426404No Hit
AAGCGGGGGACACCACCCAGGGCCACCTGGCCAGGGGTGACACTGCTCACAACAAAAACAGACA530.12757251173426404No Hit
AAGCGGGGGAGTCAGTCTTTTCCGGACTCCGCCATCAGCAGGAGGCGGGACGAGCCGGAGGCCG520.12516548321097604No Hit
AAGCGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC520.12516548321097604No Hit
AAGCGGGGGGACTCTTGCGCATGCGTGCTGCTGGAAGGCTCTCCGTTTCTCCGAGAACCGGGAA520.12516548321097604No Hit
AAGCGGGGGGGTTCTCCCGGAGGGAATCGTGGGACAGCCAGGGCGTCCCACTCGGAAGAGCTCG520.12516548321097604No Hit
AAGCGGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAACACCATGG490.11794439764111204No Hit
AAGCGGGGGATGGCCTACCCATTCCAACTTGGTCTACAAGACGCCACATCCCCTATTATAGAAG490.11794439764111204No Hit
AAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.11553736911782404No Hit
AAGCGGGGGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.11072331207124804No Hit
AAGCGGGGGAACCAGAGCTGAACTAAGGCTGCTCCATCAACACTCCAGGCCCCTGCCTACCCAC460.11072331207124804No Hit
AAGCGGGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACTTCTAGGGA460.11072331207124804No Hit
AAGCGGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAA460.11072331207124804No Hit
AAGCGGGGGGGCAGAGTCTTCAGGCTTGAGAGTGTGGGCCAGTGGGTAGGGGGCTGGGACGCTG460.11072331207124804No Hit
AAGCGGGAGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG450.10831628354796004No Hit
AAGCGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGGTTTCGACG450.10831628354796004No Hit
AAGCGGGGGCACCGCTTCCTCCTCCTCCTCCTCCTCCTCGCCCGGCCCCAGCTGCCAGCACCAT440.10590925502467204No Hit
AAGCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAAC440.10590925502467204No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGCAAGGCCCC440.10590925502467204No Hit
AAGCGGGGGGTCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGC440.10590925502467204No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCCAGGCCCC430.10350222650138403No Hit
AAGCGGGGGGGCCCTGCTCCGCCGTCTGCCACTGCCGCCTGGGCTCACTGAGTGGTTCATCTGG430.10350222650138403No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTT258.457293E-658.07618341
CTTCATG150.004631276758.0761851
CTCTGGT150.004631276758.0761827
GTTGATG150.004631276758.0761836
TTCATGG150.004631276758.0761852
CCTACTA150.004631276758.0761844
GGGTCCG150.004631276758.0761848
ATCTTCT150.004631276758.0761844
ACAGACA150.004631276758.0761858
AAACATC150.004631276758.0761848
ACATGAT150.004631276758.0761831
ACTGACG150.004631276758.0761857
TTGTAAG150.004631276758.0761844
TGATTGT150.004631276758.0761842
TGATTCG150.004631276758.0761852
CCATGCC150.004631276758.0761839
CTCTAAC150.004631276758.0761833
GACACTG150.004631276758.0761839
GAGCATC150.004631276758.0761835
ATTATCT150.004631276758.0761846
Produced by FastQC (version 0.11.5)