FastQCFastQC Report
Fri 17 Jun 2016
SRR1526375_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526375_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40916
Sequences flagged as poor quality0
Sequence length64
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG4791.1706911721575912No Hit
AAGCGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC4381.0704858734969205No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC1900.4643660181835957No Hit
AAGCGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1680.4105973213412846No Hit
AAGCGGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA1540.3763808778961775No Hit
AAGCGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1390.33972040277641996No Hit
AAGCGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA1220.2981718643073614No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT1150.2810636425848079No Hit
AAGCGGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCC1100.2688434842115554No Hit
AAGCGGGGGGCAGCCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACT940.22973897741714733No Hit
AAGCGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT900.21996285071854532No Hit
AAGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGCG870.21263075569459383No Hit
AAGCGGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT860.21018672401994332No Hit
AAGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG850.2077426923452928No Hit
AAGCGGGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCT750.18330237559878776No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC740.18085834392413724No Hit
AAGCGGGGCAAGGGACACCGAAAAGCAGTGTCTTATGCCAAGTTTGTAAGTGGTGAAGAAATTG670.16375012220158372No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT660.16130609052693323No Hit
AAGCGGGGGGACTAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAA620.15152996382833123No Hit
AAGCGGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC550.1344217421057777No Hit
AAGCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACGC540.1319777104311272No Hit
AAGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG540.1319777104311272No Hit
AAGCGGGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACA510.12464561540717568No Hit
AAGCGGGGGGAGGCTCTCCGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGC510.12464561540717568No Hit
AAGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCCC500.12220158373252518No Hit
AAGCGGGGGAGCTTTTGGAAAGCTCTTTTTTACATAATAAAAAATATACAGAAAACAGTGCTTC500.12220158373252518No Hit
AAGCGGGGGCAGTCTGCTGCTGGAACACATACATCGATCTTTATTCTCCACTGATTGGTTGCCC490.11975755205787468No Hit
AAGCGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCTC490.11975755205787468No Hit
AAGCGGGGGAAGATGAAGAGTTAAGTGACCTTAATTAACCTGTTCTGTGCCCCATCCTAAGGCA480.11731352038322418No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGCAAGGCCCC480.11731352038322418No Hit
AAGCGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC470.11486948870857366No Hit
AAGCGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC460.11242545703392316No Hit
AAGCGGGGGGGGTGGAAATTATCCTGCCACACAACACTGGCAAAACCTTGTCTACTCACAGAGA450.10998142535927266No Hit
AAGCGGGGGGTCAGTGCTGCCCGTCACAGAGATACACCCTGGAGTTACCAATAATAGCAGATCT430.10509336200997166No Hit
AAGCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAAC430.10509336200997166No Hit
AAGCGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG430.10509336200997166No Hit
AAGCGGGGGACACCACCCAGGGCCACCTGGCCAGGGGTGACACTGCTCACAACAAAAACAGACA430.10509336200997166No Hit
AAGCGGGGGGGCAGAGTCTTCAGGCTTGAGAGTGTGGGCCAGTGGGTAGGGGGCTGGGACGCTG420.10264933033532116No Hit
AAGCGGGGGGTATGTGTTTGGTTAAAGAAACTCTCTGAAATTACTGTTATAATAAGTTTTTAAA410.10020529866067066No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATGT150.004540800658.3638645
TATACTA150.004540800658.3638644
TTATCTC303.515761E-758.3638647
TTATCAC303.515761E-758.3638638
CAATTAT303.515761E-758.3638644
AACCTGT150.004540800658.3638637
TGGAGCG150.004540800658.3638644
ATTATCT303.515761E-758.3638646
ATTATAT150.004540800658.3638650
TATCTCA303.515761E-758.3638648
GCATGTG150.004540800658.3638646
TATCACA303.515761E-758.3638639
ATACTAT150.004540800658.3638645
ACTATTA150.004540800658.3638647
GCGAGCT150.004540800658.3638650
GCGAGAG150.004540800658.3638644
CCGCGCT150.004540800658.3638638
TAAATCA150.00456313258.29171856
CCTAAGG150.00456313258.29171856
TGATTTA303.5460653E-758.29171834
Produced by FastQC (version 0.11.5)