##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526375_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	40916
Sequences flagged as poor quality	0
Sequence length	64
%GC	54
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.176239124059045	39.0	38.0	40.0	35.0	40.0
2	38.18643073614234	39.0	38.0	40.0	35.0	40.0
3	37.81838400625672	39.0	38.0	40.0	34.0	40.0
4	37.664092286636034	39.0	38.0	40.0	33.0	40.0
5	37.853187017303746	39.0	38.0	40.0	35.0	40.0
6	37.88212435233161	39.0	38.0	40.0	35.0	40.0
7	38.207644931078306	39.0	38.0	40.0	35.0	40.0
8	37.443371786098346	39.0	37.0	40.0	33.0	40.0
9	37.50322612181054	39.0	37.0	40.0	32.0	40.0
10	36.711702023658226	39.0	35.0	40.0	31.0	40.0
11	36.68518427998827	39.0	35.0	40.0	31.0	40.0
12	36.85150063544823	39.0	35.0	40.0	32.0	40.0
13	36.91262586763124	39.0	36.0	40.0	32.0	40.0
14	36.830237559878775	39.0	36.0	40.0	32.0	40.0
15	36.83458793625965	39.0	36.0	40.0	32.0	40.0
16	36.45828037931371	38.0	36.0	40.0	31.0	40.0
17	36.18107830677486	38.0	35.0	40.0	30.0	40.0
18	36.45632515397399	38.0	35.0	40.0	31.0	40.0
19	36.3129093753055	38.0	35.0	40.0	30.0	40.0
20	35.93354677876625	38.0	35.0	40.0	29.0	40.0
21	36.21768990126112	38.0	35.0	40.0	30.0	40.0
22	36.15959526835468	38.0	35.0	39.0	30.0	40.0
23	35.98328282334539	38.0	35.0	39.0	30.0	40.0
24	35.62679636328087	38.0	35.0	39.0	29.0	40.0
25	35.59038029132858	38.0	35.0	39.0	29.0	40.0
26	35.67863427510021	38.0	35.0	40.0	29.0	40.0
27	35.16915143220256	38.0	35.0	40.0	27.0	40.0
28	35.09341089060514	38.0	35.0	39.0	27.0	40.0
29	34.8727637110177	38.0	34.0	39.0	27.0	40.0
30	34.901774366995795	38.0	34.0	39.0	27.0	40.0
31	34.37579431029426	38.0	33.0	39.0	25.0	40.0
32	34.574591846710334	38.0	34.0	39.0	26.0	40.0
33	34.31330042037345	38.0	33.0	39.0	25.0	40.0
34	33.62689412454785	38.0	33.0	39.0	22.0	40.0
35	33.63432398083879	38.0	33.0	39.0	22.0	40.0
36	32.32278326327109	37.0	31.0	39.0	6.0	40.0
37	31.877382930882785	36.0	31.0	39.0	4.0	40.0
38	31.7811125232183	36.0	30.0	39.0	4.0	40.0
39	31.384470622739272	36.0	30.0	39.0	4.0	40.0
40	31.420960015641803	36.0	30.0	39.0	4.0	40.0
41	30.892902532016816	36.0	29.0	39.0	4.0	40.0
42	30.632710919933523	36.0	29.0	39.0	4.0	40.0
43	30.48125427705543	35.0	29.0	39.0	4.0	40.0
44	30.331019650014664	35.0	28.0	39.0	4.0	40.0
45	30.15441392120442	35.0	28.0	39.0	4.0	40.0
46	29.71307068139603	35.0	28.0	39.0	4.0	40.0
47	29.46517254863623	35.0	27.0	39.0	4.0	40.0
48	29.41462997360446	35.0	27.0	39.0	4.0	40.0
49	29.01630169126992	35.0	26.0	38.0	4.0	40.0
50	28.591944471600353	34.0	24.0	38.0	4.0	40.0
51	28.188214879264834	34.0	23.0	38.0	4.0	40.0
52	28.035291817381953	34.0	23.0	38.0	4.0	40.0
53	27.62300811418516	33.0	21.0	38.0	4.0	40.0
54	27.249486753348325	33.0	19.0	38.0	4.0	39.0
55	27.145346563691465	33.0	17.0	38.0	4.0	39.0
56	26.4678365431616	33.0	4.0	38.0	4.0	39.0
57	26.1251833023756	33.0	4.0	37.0	4.0	39.0
58	25.889383126405317	33.0	4.0	37.0	4.0	39.0
59	25.51307556945938	32.0	4.0	36.0	4.0	39.0
60	25.318775051324664	32.0	4.0	36.0	4.0	39.0
61	25.03580506403363	32.0	4.0	36.0	4.0	39.0
62	24.788297976341774	32.0	4.0	36.0	4.0	39.0
63	24.565548929514126	32.0	4.0	36.0	4.0	39.0
64	24.455029817186432	32.0	4.0	36.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
2	1	-0.9196964721447998
2	2	0.13357444421748
2	3	-0.41021397214790767
2	4	0.2917268240931179
2	5	0.12455053596560361
2	6	-0.4823247472844656
2	7	-0.035197264082498236
2	8	0.5059355414145656
2	9	-0.01515108063551196
2	10	0.09333909736987778
2	11	0.15887605463211685
2	12	0.045378970154828835
2	13	-0.019006798168902606
2	14	-1.1310339359640125
2	15	0.665025071646582
2	16	-0.36766936572273323
2	17	0.32482301911408484
2	18	-1.0003519177659967
2	19	-0.29305921188126405
2	20	-0.48607836759963874
2	21	-0.26903348503470426
2	22	0.08787508630830843
2	23	-0.05539385253205609
2	24	0.24414836438197085
2	25	-0.11810490624336012
2	26	1.0353900534356555
2	27	1.0790904573474407
2	28	1.416111500763023
2	29	1.0068284979130269
2	30	1.1929483269359338
2	31	0.899142990095541
2	32	1.3050565903127946
2	33	0.5861148490960488
2	34	0.7017056345203656
2	35	0.9221035582185451
2	36	-2.194952864009256
2	37	-1.508303819803519
2	38	-1.2766586111698786
2	39	-3.714746817810699
2	40	-2.0717811535500523
2	41	-0.9496380414471268
2	42	-1.4463362407052713
2	43	-1.548873471658208
2	44	-0.19883055000381233
2	45	-2.0732886979203755
2	46	-3.480239065769993
2	47	-2.7608668080324605
2	48	-2.684150053002707
2	49	-2.760424167912202
2	50	-3.672590006767777
2	51	-3.892568236006216
2	52	-3.3991346505781586
2	53	-2.789929671518941
2	54	-3.376386291154489
2	55	-3.2963228403279103
2	56	-2.012605784881316
2	57	-1.1180718222497568
2	58	-3.3300279602856584
2	59	-3.2774622808620038
2	60	-1.8285296840936134
2	61	-2.880324536502062
2	62	-3.4557495568480583
2	63	-2.411442590364903
2	64	-2.1451275488703594
3	1	-0.6360707411506539
3	2	0.32217093544555553
3	3	-0.9847753756566746
3	4	-0.5664603104098092
3	5	-0.6415313353794261
3	6	-0.22647679406809118
3	7	-0.6726241646672975
3	8	-0.5671638737901077
3	9	-1.6935136537349251
3	10	-0.8174796160804192
3	11	-0.692001138350335
3	12	-1.2718724918334772
3	13	-2.089182236765396
3	14	-1.9278175616950008
3	15	-0.6990100160727124
3	16	-0.47147053531337235
3	17	0.047045241336306276
3	18	-1.976960104900499
3	19	-0.6015387440450084
3	20	-1.298943864675664
3	21	-0.8859925493621859
3	22	-0.42820678503672127
3	23	-0.589019583526202
3	24	-1.9868457876648122
3	25	-1.898806660629326
3	26	-0.8491128705409565
3	27	-0.21769316838354769
3	28	-0.3938300197048079
3	29	-0.531183197993407
3	30	-1.1447709713096756
3	31	0.07750556319495416
3	32	-0.8162884389269749
3	33	-0.19312491698582335
3	34	-0.059990271912383264
3	35	0.8387702248852094
3	36	-4.187642922488788
3	37	-3.2115201940725235
3	38	-3.8058983772517507
3	39	-20.03784623301538
3	40	-4.884646650626074
3	41	-4.179170205189816
3	42	-4.493119866436267
3	43	-4.2184641149330595
3	44	-3.644736982752349
3	45	-4.209253610201078
3	46	0.10601824417152628
3	47	-0.2857206092020519
3	48	0.8480136896873525
3	49	0.19425419466089622
3	50	1.2879363090216955
3	51	1.3969054482043113
3	52	0.9882922500066371
3	53	1.559485533159421
3	54	2.0505142936408305
3	55	0.7636186801399276
3	56	-0.030149644530439446
3	57	-0.020118605875488527
3	58	0.0690948467318826
3	59	0.9608418127052474
3	60	0.07497908783621199
3	61	-0.17272219732077687
3	62	0.4886948875963846
3	63	-0.06202738568654098
3	64	0.10633443943373422
4	1	0.8321832271033145
4	2	0.9245518878265031
4	3	1.117605576724273
4	4	-0.260904754854252
4	5	1.0140242201761325
4	6	1.3838406662493625
4	7	0.738486946443814
4	8	1.6217250150987752
4	9	0.8668038065825314
4	10	1.459504510903706
4	11	1.5032369568877613
4	12	1.1701910002300124
4	13	0.8727225251393662
4	14	0.9253570414796002
4	15	1.4462280791653797
4	16	1.8150374011945658
4	17	2.453394447685511
4	18	1.0071668792264887
4	19	1.9595723670661087
4	20	2.174072008340211
4	21	0.49337253000289394
4	22	0.30592019909026646
4	23	0.4498693053626823
4	24	0.1163288155097888
4	25	0.17738381556115002
4	26	0.5707283993003145
4	27	1.2069100062196227
4	28	1.3950588691840764
4	29	1.4196104528002422
4	30	1.7004671239284193
4	31	1.4314738171632087
4	32	0.8606956880571559
4	33	0.7267163528554477
4	34	1.6979462360241229
4	35	-0.9778964417814535
4	36	0.15918247433661037
4	37	0.6067337741814427
4	38	0.10680003544666405
4	39	1.1438997987306543
4	40	1.0169406509612244
4	41	2.0962266202070055
4	42	2.88148330816691
4	43	2.418043821574873
4	44	0.9417709537555865
4	45	2.7883654374179656
4	46	-0.2757277875745068
4	47	0.5436444701630272
4	48	1.5940454357190994
4	49	0.22679387720057775
4	50	0.9093648804502656
4	51	1.2683340196328814
4	52	2.0128954246098125
4	53	1.2205966442705325
4	54	2.4251174682440038
4	55	1.1533012198224668
4	56	2.3746122602314657
4	57	3.775913140156259
4	58	3.228618656255694
4	59	2.818778320641755
4	60	4.251169564026686
4	61	4.839976215377636
4	62	4.7728218717233695
4	63	3.6562265825674274
4	64	4.11427094737024
5	1	-0.17201845356895262
5	2	0.5623670138769228
5	3	0.1965971733629317
5	4	0.533212892204574
5	5	-0.5565640151179849
5	6	-0.7346467287086185
5	7	-0.8850424653208862
5	8	-1.178274984901222
5	9	-1.3928600589636844
5	10	-0.12957111934839105
5	11	-2.2900403540366128
5	12	-2.6264476552321696
5	13	-2.079378315196763
5	14	-2.70069337868847
5	15	-1.282343349406048
5	16	-18.293365960149973
5	17	-3.439882863238857
5	18	-3.98022807875671
5	19	-4.652192338816246
5	20	-5.2662641261135725
5	21	-4.046123268316435
5	22	-3.828533582422338
5	23	-4.597189518166729
5	24	-4.4508980752465135
5	25	-3.153708621413635
5	26	-0.6317926091030444
5	27	0.06825454403475106
5	28	0.5244706338899618
5	29	-1.3971962698888305
5	30	0.2130721659452206
5	31	-1.4584421492233446
5	32	-0.8048505304302438
5	33	-1.0052164202537952
5	34	-0.7936504026313358
5	35	-6.313190559428513
5	36	-2.091237693730619
5	37	-3.5562914359025903
5	38	-3.7797545864020776
5	39	-2.4283691088323742
5	40	-3.322555147358102
5	41	-3.9357061529022346
5	42	-17.427760389312084
5	43	-6.937418363299074
5	44	-4.231338289941892
5	45	-3.68474380627951
5	46	-3.179929468246776
5	47	-3.725263092862182
5	48	-4.910156244953171
5	49	-3.826987635404464
5	50	-2.4914754556841885
5	51	-0.6030945517956887
5	52	-0.7551718022809446
5	53	-0.8441092380824102
5	54	-0.14883211158792875
5	55	0.3600239088980963
5	56	-0.5497574876676943
5	57	-0.38122971698659924
5	58	1.4563497486926664
5	59	0.6683581525745268
5	60	0.43772418587542816
5	61	2.2357745347053672
5	62	1.3089563255048837
5	63	1.2990837254245697
5	64	1.7697331322441912
6	1	-0.35054102538837384
6	2	0.9597678346703589
6	3	0.29235640728906276
6	4	0.9716533846806286
6	5	0.41402422017613105
6	6	0.7379934901696288
6	7	0.3570915976066047
6	8	0.6589343174243609
6	9	0.6721194212004704
6	10	0.6973782650565354
6	11	0.3816422725023756
6	12	1.2093936580373281
6	13	0.7790348174981716
6	14	0.07153644347295796
6	15	0.17114502268697862
6	16	0.33796098923443196
6	17	0.9411020888815287
6	18	0.36929312507366063
6	19	0.7363165531126157
6	20	1.1222447658152888
6	21	-0.07074707132601077
6	22	0.6913022588909286
6	23	0.7707995379208228
6	24	-0.8537708522642973
6	25	-0.5654733272959902
6	26	1.0610938478052958
6	27	1.758405022830921
6	28	0.7159891017422169
6	29	0.8887134428334633
6	30	-0.07694151394533577
6	31	0.9909422557014196
6	32	1.024150837558814
6	33	0.8682445920581046
6	34	1.7843249735656528
6	35	0.561638441939472
6	36	-2.210252741610237
6	37	-2.6643625713335055
6	38	-2.5642963100682863
6	39	-17.51523641389393
6	40	-4.486713834088608
6	41	-4.0971288615205665
6	42	-4.511207721733424
6	43	-4.519497706664325
6	44	-4.87018917913478
6	45	-3.5910365559375137
6	46	-0.4863590168103862
6	47	0.1516178920899378
6	48	0.7834142064832186
6	49	0.008853677865030107
6	50	1.514680495068209
6	51	0.47830079704151984
6	52	-0.45421420994168216
6	53	0.7461780396193696
6	54	0.21847294997158073
6	55	1.5433344424138298
6	56	0.7334162469424292
6	57	1.5373749341761922
6	58	1.34622662967762
6	59	0.32841287213677006
6	60	2.3734287002393124
6	61	0.3595775442812936
6	62	0.660529512919382
6	63	0.0781534928664307
6	64	1.8983241035164191
7	1	-1.0775912089869095
7	2	-0.2611623978877802
7	3	-1.0154771300426404
7	4	-0.2082731759068821
7	5	-0.6386073587712389
7	6	-0.35074579991604793
7	7	-0.3246709482930257
7	8	-0.2835381427959618
7	9	-0.27830897537235444
7	10	-1.2750819552617045
7	11	-0.7358607874731433
7	12	-0.059884187739911
7	13	-0.834796271853115
7	14	-1.9994549885955877
7	15	0.19134086112026694
7	16	-0.28872199730167836
7	17	0.035349334903557406
7	18	0.13122702960242805
7	19	-0.42463815924968173
7	20	-1.1176573149680635
7	21	-1.321665063982067
7	22	-2.4384407031653765
7	23	-1.2132885893741587
7	24	-2.966377951407509
7	25	-2.223368064138093
7	26	-1.254083630774872
7	27	-0.026172700547292038
7	28	-0.7944148150264496
7	29	-1.098434659981713
7	30	-1.2281043046430113
7	31	-5.574541220430774
7	32	-5.642311830739839
7	33	-6.887569361430266
7	34	-22.061452260216477
7	35	-7.709475389149876
7	36	-8.221268653482941
7	37	-7.876724872435098
7	38	-6.829290190117245
7	39	-7.162115238863333
7	40	-7.019149574602682
7	41	-2.8706906730260755
7	42	-2.4989678196526413
7	43	-2.364662945342417
7	44	-2.7514621289511787
7	45	-2.520657118973009
7	46	-0.559186434191048
7	47	-0.02402470276930302
7	48	-0.631518474055337
7	49	-0.9446346942279931
7	50	0.301094203758538
7	51	-2.3136208675851613
7	52	-2.162292545315001
7	53	-2.132034934676838
7	54	-2.61322839641765
7	55	-1.2700070508542254
7	56	-0.6441847322497374
7	57	-0.27596655909186296
7	58	-1.1721332234435557
7	59	0.3804324559800989
7	60	-1.249582315672562
7	61	-0.6434824312389047
7	62	-0.008381135795428207
7	63	-0.7272320640491152
7	64	-0.5135486015019382
8	1	0.10661931732887808
8	2	0.63357444421748
8	3	0.1687333962731472
8	4	1.0548847188299604
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118	21	0.8076582442886036
118	22	0.24877734194740952
118	23	-0.178702123208744
118	24	0.48775738693836246
118	25	-0.07975904158170266
118	26	0.9135855421574561
118	27	1.3783385776481936
118	28	0.19505886918408066
118	29	2.1338961670859575
118	30	-0.5852471617858654
118	31	0.8600452457346393
118	32	1.0035528309142947
118	33	0.18385920999830319
118	34	0.9836605217384076
118	35	0.9078178439328326
118	36	1.0734681886223214
118	37	0.6353052027528747
118	38	1.4210857497323772
118	39	0.6296140844449418
118	40	1.9312263652469426
118	41	2.2676551916355834
118	42	2.5100547367383363
118	43	3.503758107289162
118	44	1.5703423823270164
118	45	2.1883654374179713
118	46	2.6957007838540648
118	47	2.3150730415915994
118	48	1.7654740071476702
118	49	1.055365305772007
118	50	0.995079166164551
118	51	-0.5888088375099727
118	52	0.8986097103240986
118	53	1.6491680728419595
118	54	1.7965460396725774
118	55	1.4390155055367515
118	56	2.2317551173743198
118	57	2.490198854441971
118	58	2.828618656255692
118	59	3.447349749213185
118	60	2.3083124211695427
118	61	2.5256905010919226
118	62	2.687107586009084
118	63	2.684798011138856
118	64	3.0571280902273834
119	1	0.0583737032937961
119	2	0.32217093544555553
119	3	-0.09588648676778888
119	4	0.3224285784790837
119	5	0.10846866462057392
119	6	-0.3098101274014269
119	7	0.049598057554931074
119	8	0.2939472373209995
119	9	0.1953752351539606
119	10	0.12696482836402367
119	11	-0.4697789161281136
119	12	0.06146084149985853
119	13	0.6052622076790541
119	14	-0.733373117250558
119	15	0.3009899839272876
119	16	-0.027026090868929487
119	17	1.1026007968918634
119	18	0.30081767287727956
119	19	-0.15709429960055843
119	20	-0.7433883091201068
119	21	0.5306741173044784
119	22	-0.37265122948116414
119	23	-0.36679736130398055
119	24	0.013154212335187765
119	25	-0.17658443840710447
119	26	0.17866490723682205
119	27	1.0045290538386737
119	28	-1.0049411308159222
119	29	0.4132612464510359
119	30	-1.2003265268652328
119	31	-0.14471665902726727
119	32	-0.8440662167047535
119	33	0.2513195274586195
119	34	-0.3655458274679404
119	35	0.5054368915518808
119	36	1.3679126330667657
119	37	1.0662575837052515
119	38	1.4996571783038064
119	39	1.906598211429067
119	40	0.865353349373926
119	41	0.23749646147685155
119	42	0.5902134668970653
119	43	1.0315358850669405
119	44	0.4663741283587619
119	45	1.1796352786878117
119	46	2.133796021949305
119	47	1.8531682796868374
119	48	1.0980136896873525
119	49	0.9442541946608962
119	50	2.2323807534661384
119	51	1.8691276704265327
119	52	1.8771811388955264
119	53	2.392818866492753
119	54	1.772736515863052
119	55	2.069174235695481
119	56	2.025405911025114
119	57	2.5632147274578436
119	58	1.5690948467318826
119	59	2.266397368260801
119	60	1.852756865613987
119	61	2.577277802679223
119	62	2.766472665374163
119	63	2.521305947646791
119	64	1.8841122172115128
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	57.0
5	24.0
6	16.0
7	21.0
8	49.0
9	41.0
10	90.0
11	78.0
12	96.0
13	163.0
14	229.0
15	282.0
16	311.0
17	389.0
18	422.0
19	506.0
20	630.0
21	815.0
22	1017.0
23	659.0
24	697.0
25	634.0
26	697.0
27	819.0
28	901.0
29	1072.0
30	1181.0
31	1452.0
32	1662.0
33	2068.0
34	2622.0
35	3209.0
36	4098.0
37	4865.0
38	5215.0
39	3810.0
40	19.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	100.0	0.0	0.0
2	0.0	100.0	0.0	0.0
3	100.0	0.0	0.0	0.0
4	0.0	0.0	0.0	100.0
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	98.62889823052107	1.0509336200997164	0.12708964708182618	0.19307850229738976
8	98.4613879308236	1.0175876323964679	0.3033193904258702	0.217705046354052
9	92.93186039691075	4.284387525662332	1.3271091993352235	1.4566428780917
10	55.66770945351451	28.250562127285168	6.3349301006941054	9.746798318506208
11	36.643366898035	25.43259360641314	17.86587154169518	20.05816795385668
12	36.08368364454003	16.682960211164335	25.584123570241466	21.64923257405416
13	26.615504936943985	18.00762537882491	27.715319190536707	27.6615504936944
14	22.367777886401406	19.37872714830384	31.183400136865774	27.070094828428974
15	25.789962825278813	18.893073762473097	30.490608491488945	24.826354920759147
16	30.720152537400995	20.27476288256576	22.861054072553046	26.1440305074802
17	29.90626759012261	20.834047135410295	20.74594356476836	28.513741709698735
18	29.372372665949754	22.125818750611007	21.607684035585102	26.894124547854144
19	28.292110665754226	21.135985922377554	24.242350180858345	26.32955323100987
20	25.578211334409072	23.566084788029926	25.69067527260281	25.165028604958195
21	26.9454492130218	18.831264053182128	28.947111154560563	25.276175579235506
22	28.485189168051615	18.89236484504839	27.400039104506796	25.222406882393194
23	27.683546778766257	23.474924235018086	25.926288004692537	22.915240981523123
24	24.243535220218018	27.127633572860145	22.840592462237865	25.788238744683973
25	25.792253521126764	23.190532081377153	22.542546948356808	28.47466744913928
26	25.524945610990248	18.594930210956022	25.207167127038062	30.672957051015672
27	22.793271467397958	18.953992311647607	28.733380671384147	29.519355549570285
28	24.74231852123883	20.48475945648182	24.40690414983474	30.366017872444605
29	24.409044193216857	22.250770811921893	26.596681838202908	26.74350315665835
30	26.528913479900112	19.279733633648338	28.80575821377858	25.38559467267297
31	25.424932647563065	20.86211119275043	28.888072495713935	24.824883663972567
32	29.37317962650219	18.863352669065275	27.246249112759134	24.517218591673398
33	28.550089379729165	19.783529642236207	28.983519847197392	22.68286113083723
34	23.152395202236995	21.92106747774044	33.64811498933013	21.278422330692436
35	23.854087557265	25.02021117616796	30.60339547759622	20.522305788970822
36	27.053400676702786	22.669543470798803	26.771441180797336	23.505614671701075
37	25.350074797066974	24.327439487946638	28.50871814993746	21.813767565048924
38	27.730783376241263	20.568836582076745	29.57459850435209	22.1257815373299
39	26.120119085697414	20.237187215510666	26.40553108776419	27.237162611027728
40	26.752998307703628	21.631962328011184	27.976847423540086	23.638191940745102
41	21.56877177486628	21.649737474851563	29.62853918249178	27.152951567790375
42	22.50251774704625	18.307091449485398	33.462700498636735	25.72769030483162
43	23.257527383466773	17.739083452035956	35.296429097696354	23.70696006680092
44	26.551453260015712	19.400530243519245	30.572466614296935	23.475549882168107
45	24.25276911363804	18.181594911216443	29.872043618144755	27.693592357000764
46	25.2941320953995	19.61044383857736	30.029720236779404	25.06570382924373
47	22.985485178181104	21.551194832625193	32.33047621386645	23.13284377532726
48	22.966037179833503	23.346676162176763	29.122565752314532	24.564720905675205
49	24.16292038224384	25.037462844228266	27.091163681922026	23.708453091605865
50	20.96992351769422	27.40083122248727	26.825369500528733	24.803875759289774
51	22.497174030569617	29.205779721826314	26.95729100113039	21.339755246473683
52	25.589341461615987	26.21125341068312	27.880337258179495	20.319067869521398
53	28.029408871840268	23.48037769253467	27.43434641487164	21.055867020753418
54	24.23810460086512	25.12042862760519	26.48692489186001	24.15454187966968
55	29.384713751721424	22.653944520952194	26.308282510328546	21.653059216997836
56	22.62809632745431	24.244213219196613	27.29441861609229	25.83327183725678
57	22.883565349992622	26.79423483693246	26.9639431354223	23.358256677652616
58	23.00972068413929	28.52467085025225	26.016980435585086	22.44862803002338
59	24.99138737142576	27.479206653870765	26.667158816870913	20.86224715783257
60	26.490066225165563	25.773652724094635	24.734730052438515	23.001550998301287
61	24.380287231949634	24.399960653157585	24.891796183356284	26.327955931536493
62	23.43911439114391	23.389913899138993	25.057810578105784	28.113161131611314
63	22.26623041157224	23.535634332947918	25.102708553715956	29.095426701763884
64	25.73560190086918	22.315514736660674	24.33211040799744	27.616772954472708
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	1.5
8	2.0
9	15.5
10	40.5
11	52.0
12	53.5
13	43.5
14	32.0
15	72.0
16	135.0
17	158.0
18	238.0
19	318.0
20	327.5
21	327.0
22	317.0
23	404.0
24	514.0
25	537.0
26	589.5
27	677.0
28	712.0
29	727.5
30	642.5
31	542.0
32	551.5
33	561.0
34	687.0
35	664.0
36	515.0
37	499.0
38	435.5
39	388.0
40	448.5
41	546.0
42	583.0
43	566.5
44	550.0
45	643.0
46	807.5
47	879.0
48	878.0
49	896.5
50	916.0
51	1046.0
52	1163.0
53	1150.0
54	1182.5
55	1461.0
56	1707.0
57	1794.5
58	1882.0
59	1842.0
60	2193.0
61	2584.0
62	2589.5
63	2689.5
64	2784.0
65	2572.0
66	2313.5
67	2267.0
68	2455.5
69	2644.0
70	2036.5
71	1161.0
72	893.0
73	716.0
74	491.0
75	443.0
76	332.0
77	186.5
78	152.0
79	109.5
80	57.5
81	48.0
82	40.5
83	33.0
84	26.0
85	12.0
86	5.0
87	6.0
88	4.5
89	2.0
90	1.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.08554110861276762
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0684328868902141
16	0.01955225339720403
17	0.1344217421057777
18	0.0
19	0.0
20	0.03421644344510705
21	0.0
22	0.0
23	0.0
24	0.004888063349301007
25	0.04888063349301008
26	0.017108221722553524
27	0.18330237559878776
28	0.17352624890018575
29	0.12220158373252518
30	0.17108221722553524
31	0.21018672401994332
32	0.1441978688043797
33	0.19307850229738976
34	0.359272656173624
35	0.23707107244109885
36	0.31772411770456543
37	0.33972040277641996
38	0.32016814937921595
39	0.6672206471795875
40	0.34949652947502197
41	0.38615700459477953
42	0.5010264933033531
43	0.4839182715807997
44	0.43992570143709064
45	0.48636230325545016
46	0.49613842995405216
47	0.48636230325545016
48	0.47658617655684815
49	0.5108026200019552
50	0.6183400136865773
51	0.5425750317724118
52	0.5743474435428683
53	0.6061198553133249
54	0.5572392218203148
55	0.6158959820119269
56	0.6427803304330824
57	0.63056017205983
58	0.6867729005767915
59	0.6794408055528399
60	0.7258774073711995
61	0.6158959820119269
62	0.650112425457034
63	0.6525564571316844
64	0.7405415974191026
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
64	40916.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.48030110470232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.53112146970113	42.53348323394271
2	8.303628553148707	8.881611105679928
3	3.687962709075953	5.917000684328869
4	2.399232245681382	5.1324665167660575
5	1.5720683666940867	4.203734480398865
6	1.0602321542820583	3.4020920911135013
7	0.7403345215245407	2.771531919053671
8	0.5575358742345307	2.3853749144588914
9	0.35188739603326935	1.6937139505327987
>10	1.686317521250343	15.82999315671131
>50	0.06854949273375377	2.568677290057679
>100	0.04112969564025226	4.680320656955715
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAGCGGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAG	479	1.1706911721575912	No Hit
AAGCGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGC	438	1.0704858734969205	No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCC	190	0.4643660181835957	No Hit
AAGCGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	168	0.4105973213412846	No Hit
AAGCGGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATATAAATCAAA	154	0.3763808778961775	No Hit
AAGCGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	139	0.33972040277641996	No Hit
AAGCGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATCAATAATTA	122	0.2981718643073614	No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCT	115	0.2810636425848079	No Hit
AAGCGGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCC	110	0.2688434842115554	No Hit
AAGCGGGGGGCAGCCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGAGCTGAACT	94	0.22973897741714733	No Hit
AAGCGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	90	0.21996285071854532	No Hit
AAGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAAGAGCG	87	0.21263075569459383	No Hit
AAGCGGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCT	86	0.21018672401994332	No Hit
AAGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCCG	85	0.2077426923452928	No Hit
AAGCGGGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCT	75	0.18330237559878776	No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGCAAGGCCCC	74	0.18085834392413724	No Hit
AAGCGGGGCAAGGGACACCGAAAAGCAGTGTCTTATGCCAAGTTTGTAAGTGGTGAAGAAATTG	67	0.16375012220158372	No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT	66	0.16130609052693323	No Hit
AAGCGGGGGGACTAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGCAAA	62	0.15152996382833123	No Hit
AAGCGGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTC	55	0.1344217421057777	No Hit
AAGCGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACGC	54	0.1319777104311272	No Hit
AAGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGGCCCTG	54	0.1319777104311272	No Hit
AAGCGGGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCATGGTAGACA	51	0.12464561540717568	No Hit
AAGCGGGGGGAGGCTCTCCGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGGACATTGGC	51	0.12464561540717568	No Hit
AAGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCCC	50	0.12220158373252518	No Hit
AAGCGGGGGAGCTTTTGGAAAGCTCTTTTTTACATAATAAAAAATATACAGAAAACAGTGCTTC	50	0.12220158373252518	No Hit
AAGCGGGGGCAGTCTGCTGCTGGAACACATACATCGATCTTTATTCTCCACTGATTGGTTGCCC	49	0.11975755205787468	No Hit
AAGCGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAACACCTCCTC	49	0.11975755205787468	No Hit
AAGCGGGGGAAGATGAAGAGTTAAGTGACCTTAATTAACCTGTTCTGTGCCCCATCCTAAGGCA	48	0.11731352038322418	No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGCAAGGCCCC	48	0.11731352038322418	No Hit
AAGCGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGGCCGCCGCC	47	0.11486948870857366	No Hit
AAGCGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGGTCAACCC	46	0.11242545703392316	No Hit
AAGCGGGGGGGGTGGAAATTATCCTGCCACACAACACTGGCAAAACCTTGTCTACTCACAGAGA	45	0.10998142535927266	No Hit
AAGCGGGGGGTCAGTGCTGCCCGTCACAGAGATACACCCTGGAGTTACCAATAATAGCAGATCT	43	0.10509336200997166	No Hit
AAGCGGGGGGAGCAGCGCCTTGGAGGTCCCGTGGATCTGTGTCTTGCTTCAACAGTGTTTGAAC	43	0.10509336200997166	No Hit
AAGCGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGTGTTTCGACG	43	0.10509336200997166	No Hit
AAGCGGGGGACACCACCCAGGGCCACCTGGCCAGGGGTGACACTGCTCACAACAAAAACAGACA	43	0.10509336200997166	No Hit
AAGCGGGGGGGCAGAGTCTTCAGGCTTGAGAGTGTGGGCCAGTGGGTAGGGGGCTGGGACGCTG	42	0.10264933033532116	No Hit
AAGCGGGGGGTATGTGTTTGGTTAAAGAAACTCTCTGAAATTACTGTTATAATAAGTTTTTAAA	41	0.10020529866067066	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.00733209502395151	0.0	0.0	0.0	0.0
11	0.009776126698602014	0.0	0.0	0.0	0.0
12	0.009776126698602014	0.0	0.0	0.0	0.0
13	0.012220158373252517	0.0	0.0	0.0	0.0
14	0.01955225339720403	0.0	0.0	0.0	0.0
15	0.01955225339720403	0.0	0.0	0.0	0.0
16	0.02199628507185453	0.0	0.0	0.0	0.0
17	0.02932838009580604	0.0	0.0	0.0	0.0
18	0.02932838009580604	0.0	0.0	0.0	0.0
19	0.02932838009580604	0.0	0.0	0.0	0.0
20	0.03421644344510705	0.0	0.0	0.0	0.0
21	0.04399257014370906	0.0	0.0	0.0	0.0
22	0.05621272851696158	0.0	0.0	0.0	0.0
23	0.05621272851696158	0.0	0.0	0.0	0.0
24	0.05621272851696158	0.0	0.0	0.0	0.0
25	0.05865676019161208	0.0	0.0	0.0	0.0
26	0.0659888552155636	0.0	0.0	0.0	0.0
27	0.0708769185648646	0.0	0.0	0.0	0.0
28	0.0733209502395151	0.0	0.0	0.0	0.0
29	0.07576498191416561	0.0	0.0	0.0	0.0
30	0.08065304526346662	0.0	0.0	0.0	0.0
31	0.08554110861276762	0.0	0.0	0.0	0.0
32	0.08798514028741812	0.0	0.0	0.0	0.0
33	0.09042917196206862	0.0	0.0	0.0	0.0
34	0.09776126698602014	0.0	0.0	0.0	0.0
35	0.10264933033532114	0.0	0.0	0.0	0.0
36	0.10509336200997164	0.0	0.0	0.0	0.0
37	0.10998142535927266	0.0	0.0	0.0	0.0
38	0.11975755205787467	0.0	0.0	0.0	0.0
39	0.12220158373252517	0.0	0.0	0.0	0.0
40	0.1344217421057777	0.0	0.0	0.0	0.0
41	0.1417538371297292	0.0	0.0	0.0	0.0
42	0.1490859321536807	0.0	0.0	0.0	0.0
43	0.1490859321536807	0.0	0.0	0.0	0.0
44	0.15397399550298171	0.0	0.0	0.0	0.0
45	0.15397399550298171	0.0	0.0	0.0	0.0
46	0.15397399550298171	0.0	0.0	0.0	0.0
47	0.15641802717763223	0.0	0.0	0.0	0.0
48	0.16130609052693323	0.0	0.0	0.0	0.0
49	0.16619415387623424	0.0	0.0	0.0	0.0
50	0.17108221722553524	0.0	0.0	0.0	0.0
51	0.17352624890018575	0.0	0.0	0.0	0.0
52	0.17841431224948676	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCATGT	15	0.0045408006	58.36386	45
TATACTA	15	0.0045408006	58.36386	44
TTATCTC	30	3.515761E-7	58.36386	47
TTATCAC	30	3.515761E-7	58.36386	38
CAATTAT	30	3.515761E-7	58.36386	44
AACCTGT	15	0.0045408006	58.36386	37
TGGAGCG	15	0.0045408006	58.36386	44
ATTATCT	30	3.515761E-7	58.36386	46
ATTATAT	15	0.0045408006	58.36386	50
TATCTCA	30	3.515761E-7	58.36386	48
GCATGTG	15	0.0045408006	58.36386	46
TATCACA	30	3.515761E-7	58.36386	39
ATACTAT	15	0.0045408006	58.36386	45
ACTATTA	15	0.0045408006	58.36386	47
GCGAGCT	15	0.0045408006	58.36386	50
GCGAGAG	15	0.0045408006	58.36386	44
CCGCGCT	15	0.0045408006	58.36386	38
TAAATCA	15	0.004563132	58.291718	56
CCTAAGG	15	0.004563132	58.291718	56
TGATTTA	30	3.5460653E-7	58.291718	34
>>END_MODULE