FastQCFastQC Report
Fri 17 Jun 2016
SRR1526374_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526374_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37024
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCGGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGA5161.3936905790838374No Hit
AAGCGGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAG3821.0317631806395853No Hit
AAGCGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT1930.5212834917891098No Hit
AAGCGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1900.5131806395851339No Hit
AAGCGGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATAT1480.39974070872947276No Hit
AAGCGGGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCATC1380.3727312013828868No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCCAGCAAGCGC1130.3052074330164218No Hit
AAGCGGGGGGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1070.2890017286084702No Hit
AAGCGGGGGACTGCACTGCGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCT1060.2863007778738116No Hit
AAGCGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCACCGCGGCCAAGGAGAGC940.2538893690579084No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCCAGCAAGCGC910.24578651685393257No Hit
AAGCGGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAA870.23498271391529818No Hit
AAGCGGGGGGCAGCCAGTGAATCTCTCTTCATCTCTAACCATGCCTACTAACCAGA860.2322817631806396No Hit
AAGCGGGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG850.229580812445981No Hit
AAGCGGGGGACACCACCCAGGGCCACCTGGCCAGGGGTGACACTGCTCACAACAAA800.216076058772688No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC790.21337510803802937No Hit
AAGCGGGGGGAGGCTCTCCGTTTCTCCGAGAACCGGGAAGGACGTTGGTGTAGAGG790.21337510803802937No Hit
AAGCGGGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCTAATCGC760.2052722558340536No Hit
AAGCGGGGGAAGATGAAGAGTTAAGTGACCTTAATTAACCTGTTCTGTGCCCCATC650.17556179775280897No Hit
AAGCGGGGGATCCTCACCACCCACACCACCCTGGAGCACTCTGATTGTGCCTTCAT640.17286084701815038No Hit
AAGCGGGGGCAGTCTGCTGCTGGAACACATACATCGATCTTTATTCTCCACTGATT640.17286084701815038No Hit
AAGCGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATGG620.1674589455488332No Hit
AAGCGGGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTGTGAGGGGG610.16475799481417458No Hit
AAGCGGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGC590.15935609334485737No Hit
AAGCGGGGGCACCGCTTCCTCCTCCTCCTCCTCCTCCTCGCCCGGCCCCAGCTGCC560.1512532411408816No Hit
AAGCGGGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG540.14585133967156438No Hit
AAGCGGGGGGACTCTTGCGCATGCGTGCTGCTGGAAGGCTCTCCGTTTCTCCGAGA540.14585133967156438No Hit
AAGCGGGGGAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCTTGCAAA540.14585133967156438No Hit
AAGCGGGGGGCCTTTTCCTGTGGGAGCAGCCGGGTAGAGAGGAGCGTGGCCTTCTC530.1431503889369058No Hit
AAGCGGGGGGGCTTTGCAGACGCCACTGTCGCTTTTCGCCGCTTGCTGCAGCCATG520.1404494382022472No Hit
AAGCGGGGGGGGTGGAAATTATCCTGCCACACAACACTGGCAAAACCTTGTCTACT510.1377484874675886No Hit
AAGCGGGGGAGCTTTTGGAAAGCTCTTTTTTACATAATAAAAAATATACAGAAAAC490.1323465859982714No Hit
AAGCGGGGGGGGACGACGTTCGTCATTTGGTGCGGGAGGGAGCGCGAGAGCGGCGG490.1323465859982714No Hit
AAGCGGGGGTTTAATGCTGGCCTAACTATTACCAAGGCCATGCTGGAGAAATGGCA480.12964563526361278No Hit
AAGCGGGGGCTCTTCCTGTCTGTATCAGGGCGGCGCGTGGTCCACGCCGAGCGACT480.12964563526361278No Hit
AAGCGGGGGACGGTGAACTGACCAAGATAAAATATAAGATCAGGAGACAAAAATCT470.1269446845289542No Hit
AAGCGGGGGGCTCTCTGCTCCTCCCTGTTCCAGAGACGGCCGCATCTTCTTGTGCA470.1269446845289542No Hit
AAGCGGGGGGGTTCTCCCGGAGGGAATCGTGGGACAGCCAGGGCGTCCCACTCGGA470.1269446845289542No Hit
AAGCGGGGGGCTCTTTCCGTTCCTAGCGCAGCCATGGCTCGTGGTCCCAAGAAACA460.12424373379429558No Hit
AAGCGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA460.12424373379429558No Hit
AAGCGGGGCAAGGGACACCGAAAAGCAGTGTCTTATGCCAAGTTTGTAAGTGGTGA450.12154278305963699No Hit
AAGCGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA430.1161408815903198No Hit
AAGCGGGGGGGACCCAGCAGGGACTTCGCCTCTTAGTCTCAGCAGCTTATCTGCCC420.1134399308556612No Hit
AAGCGGGGGAGGTATGAATGACAACTGTTTGAGAAGAAGGTCCCAAATCCCTCTCT420.1134399308556612No Hit
AAGCGGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGGGCGCCTGCCCAGCAAGCGC410.1107389801210026No Hit
AAGCGGGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAGCGCGAGCT410.1107389801210026No Hit
AAGCGGGGGACCCCTAGCGTCATATGAACTGGGTATATATGTCTGTAGAGAGTCTG410.1107389801210026No Hit
AAGCGGGGGATGCCATCTACCTTCTTCAACCTCACCATAGCCTTCTCACTATCACT400.108038029386344No Hit
AAGCGGGGGAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCAAC400.108038029386344No Hit
AAGCGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTT400.108038029386344No Hit
AAGCGGGGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAAACGCACC390.10533707865168539No Hit
AAGCGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.10533707865168539No Hit
AAGCGGGGGGGCCATTACTGCCGGAAAAGGTCCAGCAGAGCTCAGCAGCCAAGATG390.10533707865168539No Hit
AAGCGGGGGGCAGGTTCTCTTACATCGACCGCCTAAGAGTCGCGCTGTAAGAAGCA380.1026361279170268No Hit
AAGCGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.1026361279170268No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTA150.00786472150.78700337
TAACTCG150.00786472150.78700337
GTGACAC150.00786472150.78700337
TCGATCT150.00790789850.7168534
TGATTTA150.00790789850.7168534
TGCGATT150.00790789850.7168536
TAACCTG150.00790789850.7168536
TCTAACC150.00790789850.7168534
ATTGCGA150.00790789850.7168534
GATCTTT150.00790789850.7168536
ATTAACC150.00790789850.7168534
TAGAGGG150.00790789850.7168534
TTGCGAT150.00790789850.7168535
TTAACCT150.00790789850.7168535
GTAACTC150.00790789850.7168536
CGATCTT150.00790789850.7168535
AGGGGTG150.00795125150.646933
TTATGCC150.00795125150.646933
AAACCAG203.8762428E-450.646933
ATCGATC150.00795125150.646933