FastQCFastQC Report
Fri 17 Jun 2016
SRR1526372_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526372_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2553
Sequences flagged as poor quality0
Sequence length64
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1797.011359185272228No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1104.308656482569527No Hit
TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTTC572.2326674500587544No Hit
TTTAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGAACTGACGG491.9193106149627888No Hit
TTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA451.762632197414806No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA271.0575793184488838No Hit
TTTAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA220.8617312965139052No Hit
TTTAGGGGGTGTTAATAAACAAGGGAAAAACCAGTTCCAGACGTTATATGTTAATAAACAAGGG170.6658832745789268No Hit
TTTAGGGGGAGCTGGTTCTCCCCGAAATGCATTTAGGTGCAGCGTTGCGTGGTTGTTGTTGGTG160.626713670191931No Hit
TTTAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.5483744614179397No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.5483744614179397No Hit
TTTAGGGGGTGTCGTTATACATCTTGGACTGCCGAACTTGCCATCAATGTAAATTGTAATACTA140.5483744614179397No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.509204857030944No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA120.4700352526439483No Hit
TTTAGGGGGGTTGTGTACAGTTCATTGCGCGACAAAAAAGTCCAGTTTCTTCATTGCCTCAAAA100.39169604386995693No Hit
TTTAGGGGGGTTATTTAGGGCGCATGGTGGATGCCTTGGCATGCTGAGCCGATGAAGGACGTGA100.39169604386995693No Hit
TTTAGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.35252643948296125No Hit
TTTAGGGGGGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGA90.35252643948296125No Hit
TTTAGGGGGGTTAAGGAACTCGGCAAAATGACCCCGTAACTTCGGGAGAAGGGGTGCTGACTTT90.35252643948296125No Hit
TTTAGGGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGA90.35252643948296125No Hit
TTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.3133568350959655No Hit
TTTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.3133568350959655No Hit
TTTAGGGGGGACCGATAGCTGCATCTTTGAGAAGGAGTAATCGTTCGGTTTAGGTGATTATTTG70.27418723070896983No Hit
TTTAGGGGGGGTCAAGTAATGAAGTGCACATGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAG70.27418723070896983No Hit
TTTAGGGGGAAGTTGTTACGGGCACACGGTGGATGCCTTGGCATCAGGAGCTGATGAAGGACGT60.23501762632197415No Hit
TTTAGGGGGGACATACTGTGAATGCCTCAATCTATGTACTCCGCCAAAAAAAAAAAAAAAAAAA60.23501762632197415No Hit
TTTAGGGGGAAAAAACCTTACATAAATTAAAGAATGAATACATTTACAGGTGTCGATGCAAACG60.23501762632197415No Hit
TTTAGGGGGAAGCTCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.23501762632197415No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.23501762632197415No Hit
TTTAGGGGGGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCG60.23501762632197415No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.23501762632197415No Hit
TTTAGGGGGAAGCTAGGGGGGCTGGAGAGATGGCTCAGCGGTTAAGGACATATTCAAAAAGACT50.19584802193497847No Hit
TTTAGGGGGATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGT50.19584802193497847No Hit
TTTAGGGGGGCTGGTGAGATGGCCCAATGGTTAAGAACACTGACTGTTCTTCCAGAGGTCCTGA50.19584802193497847No Hit
TTTAGGGGGGGAGTCGATAACCGGACGTTGAGACCAGGATTTCCGAATGGGGAAACCCCGCACG50.19584802193497847No Hit
TTTAGGAGGTTCGTGGCCGGAAAAAAATAAAAATAACCAATAAAGGAAATCATAAAAAAACAAA50.19584802193497847No Hit
TTTAGGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCG50.19584802193497847No Hit
TTTAGGGGGGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGAT50.19584802193497847No Hit
TTTAGGGGGCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGCGTACCACC50.19584802193497847No Hit
TTTAGGGGGCGTCTCTACTAAAAATACAAAAATTAGCCAGGTGTGGTGGTGCGCGTCAATAATC50.19584802193497847No Hit
TTTAGGGGGATGGACAGTCGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.19584802193497847No Hit
TTTAGGGGGGTCAAGTAATTAATGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGATGAAAGAC50.19584802193497847No Hit
TTTAGGGGGGACTGTTTAATTAAAACAAAGCATTGCGATGGTCCTCGTGATGCTGACGCAATGT50.19584802193497847No Hit
TTTAGGGGGGGGAATTCGTGGAGAAAGAAATGGCCCGTCTGGCAGCATTTGATATGGATGGCAC50.19584802193497847No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.19584802193497847No Hit
TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTGGCGCCGGAAGATGCTTTTC50.19584802193497847No Hit
TTTAGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCTTTT50.19584802193497847No Hit
TTTAGGGGGGCCGATCGACAACCTTAGCAAAGATGCAGATCTTCGTTAAGACCCTGACGGGCAA50.19584802193497847No Hit
TTTAGGGGGCGCAGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.19584802193497847No Hit
TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGATGCCTTTC50.19584802193497847No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAA40.15667841754798276No Hit
TTTAGGGGGTCCAGTTCGGTTGAAAGAAAAAAACATCAAGATACAGGGCCAACCAAAAAAATAA40.15667841754798276No Hit
TTTAGGGGGGAAACATCTAAGTAGCTGCAGGAAGAGAAAGCAAAAGCGATTGCCTTAGTAGCGG40.15667841754798276No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGCGGG40.15667841754798276No Hit
TTTAGGGGGACCCTTCAAAAAATTAATGAATCCAGGAGCTGGTTTTTTGAACGGATCAACAAAA40.15667841754798276No Hit
TTTAGGGGGTCGGACCTCCGATCAAGTAAGAGGACCCGCTGAATTTAAGCATATAACTAAGCGG40.15667841754798276No Hit
TTTAGGGGAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCCAGCAGTGG40.15667841754798276No Hit
TTTAGGAGGGAGTGCAGTGCTTTTAGGGGGGTTGGCCATCAAGATCCCCAAAAAAAAAAAAAAA40.15667841754798276No Hit
TTTAGGGGGGTGTGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.15667841754798276No Hit
TTTAGGGGGGTGAAAAGCCCGCTCGCCGAAAGCCCAAGGTTTCCTACGCAACGTTCATCGGCGT40.15667841754798276No Hit
TTTAGGGGGGAAAAGAAATCGAAGAGATTCCGTAAGTAGTGGCGAGCGAGCGGATTAGCCCAAA40.15667841754798276No Hit
TTTAGGGGGAGTGCAGTGCTTGATCAGGGGGGGCTAGAGAGATGGCTCAGCGGTTAAGAGCACT40.15667841754798276No Hit
TTTAGGGGGCGGAAGGTTGGTGTGTTAATTCGGAGTGACTCGTCTTTTCCCAAATGATAAGTGG40.15667841754798276No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGG40.15667841754798276No Hit
TTTAGGGGGGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGG40.15667841754798276No Hit
TTTAGGGGGGTTGTGTACAGTTCATTGCGCGACAAAAAAGTCCAGTTTCCTCATTGCCTCAAAA40.15667841754798276No Hit
TTTAGGGGGGCCATCGCCGTTCGACTGGGTTGATTGATTACCGCACAGGATTAAAGCACCAGGC40.15667841754798276No Hit
TTTAGGGGGATCTTGATGGCCACACCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.15667841754798276No Hit
TTTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.15667841754798276No Hit
TTTAGGGAAAAAAACTTTATTAGCTTTCTGCTTCTCATAATCACACATCATCTTTAAGGAGTTA40.15667841754798276No Hit
TTTAGGGGGGGCTGTTTTTTCGGCTCCTCGGGTTTGTGTCTGCAGGTGCCATCCGCGAAGATGC40.15667841754798276No Hit
TTTAGGGGGCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTT40.15667841754798276No Hit
TTTAGGGGGGGTCAAGAAAGAAAGCGCAAACGGTGGATGCCTAGGCAATATACGGCGAAGAAGG40.15667841754798276No Hit
TTTAGGGGGTGGAGCTGTGGAGCCTCCTGGTCTCCAGCATCAGTTCCTGGGTCCTGTCCGAAGG40.15667841754798276No Hit
TTTAGGGGGGATCAGAGTATGTCTTGTGTGTGAGAGAGCCTTACAATCATGTCCACTGAAGCCA40.15667841754798276No Hit
TTTAGGGGGATAGTTATTGGCAGCATCCTCCTTGCCTGTGATGAGCTGCTCAGGGGGGAAGAGC40.15667841754798276No Hit
TTTAGGGGGGCTCACACCCATAATCCCAGCACTTTGGGAGGCTGAGGTGGGTAGATTACTTGAG40.15667841754798276No Hit
TTTAGGGGGACTGTACGGGTATAACTAAATTTAATCAGATCTATGTTATTGGCAATTTAAATTA40.15667841754798276No Hit
TTTAGGGGGATCTTGATGGCAATTTAAATAGTTTTATTATTCCATGTTACTTGTTTTGGGTCCC30.11750881316098707No Hit
TTTAGGGGGGCCAAGGTGTTGGAGCAGCTCACAGGGCAGACCCCTGTGTTTTCCAAAGCTAGAT30.11750881316098707No Hit
TTTAGGGGGGTGCCGAGGATCGGGAACATCCGGTAGCGCCCCGTGCGGGCGATGATCTGCCCGC30.11750881316098707No Hit
TTTAGGGGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAATAAGCGGAGG30.11750881316098707No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11750881316098707No Hit
TTTAGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11750881316098707No Hit
TTTAGGGGGCAGTGTATAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.11750881316098707No Hit
TTTAGGGGGAGGAGAATTGCGTGTACCCGGAAGGCGGAGCTTGCAGTGAGCCGAGATTGCGCCA30.11750881316098707No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAGCAGGGTTTATTTTTCTATCAAATTCCCAAT30.11750881316098707No Hit
TTTAGGGGGATCTTGATGGCCGACATCGAGCAGGTTTTCAGGTCTTTCGTGGTCAGTAAATTCC30.11750881316098707No Hit
TTTAGGGGGACCTGTGGTCACTAAACCCATACAACCAAATTAAAGATGTAAACGAATAAAGTGT30.11750881316098707No Hit
TTTAGGGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAATAAGCGGA30.11750881316098707No Hit
TTTAGGGGGAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11750881316098707No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11750881316098707No Hit
TTTAGGGGGCGGCGGCCGTAACGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT30.11750881316098707No Hit
TTTAGGGGGGAATTGATCCGCAGATGTCCGAATGGGGCAACCCGGCATGTTGAAGACATGTCAC30.11750881316098707No Hit
TTTAGGGGGAAGCAGTGATATCAACGCAGAATGCCAAGTCAAAGAATCATGTACTCATTATACT30.11750881316098707No Hit
TTTAGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCCCCTGGCAGCATTTGATATGGATGGGAC30.11750881316098707No Hit
TTTAGGGGCACGTGTAGGGATCACATTCACCTTTAAAAGATGCTGTGGGAGCTGGGCAGTGGTG30.11750881316098707No Hit
TTTAGGGGTGAAAAAAAAAAGGTAACTTTAATGCAGTAATAACAATCTGAGGATAAATAAATCC30.11750881316098707No Hit
TTTAGGGGGGGTAAAGTGATGTTCGAAATCGCTGGTGTATCTGAAGAGATCGCTCGCGAAGCGC30.11750881316098707No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG30.11750881316098707No Hit
TTTAGGGGGGTGTGGCCATAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11750881316098707No Hit
TTTAGGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11750881316098707No Hit
TTTAGGGGGAAGACGCAGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA30.11750881316098707No Hit
TTTAGGGGGGGGCTGCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCG30.11750881316098707No Hit
TTTAGGGGGGGTCCTTGCCGTATGATTGCCCCGTTCTTCGCTGAGCTAGCTAATAAGTTCACCG30.11750881316098707No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAA30.11750881316098707No Hit
TTTAGGGGGGCAGAGATAGAGGAATAAGCTGGGAAGCTTAGCGACACAGGGTGATAGCCCCGTA30.11750881316098707No Hit
TTTAGGGGGGTGAACTTGTCGGAATATAGGGGTACCATCCTCTAAGGCTAAGTAATATAAATTT30.11750881316098707No Hit
TTTAGGGGATGCGTCTGTGGAGCAGCTCCTGTCTCCCAACTATATAGTAATCATTAGTAGACTG30.11750881316098707No Hit
TTTAGGGGGCCATCTCATCCCTGCGTGTCTCCGACTCAGTACGTAGAGTTTGATCCTGGCTCAG30.11750881316098707No Hit
TTTAGGGGGGTCCGGTATCCGAAGTCCCCGTGTTCGTCATGTCGGCGTCTGTGGTGTCCGTCAT30.11750881316098707No Hit
TTTAGGGGGGTGTGGCCGCCTCGTTCAATCTCTGCACCTTTCCCACAAGGAGATGGGGGTGATG30.11750881316098707No Hit
TTTAGGGGGGGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11750881316098707No Hit
TTTAGGGATCAATTGAAAGTGGATCAACATGAGAAATTCTGAAAATGGCAAAGGAAAGCTTAAG30.11750881316098707No Hit
TTTAGGGGGGAGTACAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11750881316098707No Hit
TTTAGGGCGTCGTGTGTGGGTGACATCTTGGCGATCGCTTGGAAGCTGCCCTCTTTCCCCTCCC30.11750881316098707No Hit
TTTAGGGGGAGCATTCAACAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.11750881316098707No Hit
TTTAGGGGGACCAGCCTGACCAACATGGTGAAACCCCGTCTCTAAAAAAAAAAAAAAAAAAAAA30.11750881316098707No Hit
TTTAGGGGGTGTACCCCAATTCCAAACTCCCTGAGGATGAGGCATGGGGTCCCACTAGAGAAGT30.11750881316098707No Hit
TTTAGGGGGCATGCCATGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.11750881316098707No Hit
TTTAGGGGGGAAAGCGAGTCTGAATAGGGCGAAAGTCAGGTGTTATCGACCCGAAACCAAGTGA30.11750881316098707No Hit
TTTAGGGGGGGGTCAAGCGAACAAGTGCATGTGGTGGATGCCTTGGCGATCACAGGCGATGAAG30.11750881316098707No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAGATCGGAAGAGCTCGTATGCCGT30.11750881316098707Illumina Single End Adapter 2 (100% over 24bp)
TTTAGGGGGGGAGTTGATAACCGGACGTTGAGACCAGGATTTCCGAATGGGGAAACCCCGCACG30.11750881316098707No Hit
TTTAGGGGGGAGTGTGACATGAATATTGTATTGTCAGATGAAGCCATGACTCAATTGGTCGTTT30.11750881316098707No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGC150.00416987858.0000044
TAGCGCC201.6523969E-458.044
AGGGGGT256.448143E-658.04
TTTTGCT201.6523969E-458.029
TCCTTCG256.448143E-658.016
CCGGAAG201.6523969E-458.049
TCGATTC201.6523969E-458.020
TTGTAGC201.6523969E-458.041
AATTCCT256.448143E-658.013
GATGCTT201.6523969E-458.055
TTCGATT201.6523969E-458.019
TGTTTTG201.6523969E-458.027
TTCTGTT201.6523969E-458.024
ACCCGTT201.6523969E-458.036
GTTTTGC201.6523969E-458.028
GAAGATG201.6523969E-458.052
CCGTTGT201.6523969E-458.038
GCTACCC201.6523969E-458.033
TTCCTTC256.448143E-658.015
CCCGTTG201.6523969E-458.037