FastQCFastQC Report
Fri 17 Jun 2016
SRR1526371_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526371_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179
Sequences flagged as poor quality0
Sequence length56
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAA2019.224414869206058No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1245.690683799908214No Hit
TTTAGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGA582.6617714547957783No Hit
TTTAGGGGGGGGAATTCCTTCGGCAACATTAACTGGTTGATGCTGAAAAACATCGA442.0192748967416243No Hit
TTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA431.9733822854520422No Hit
TTTAGGGGGTGTTAATAAACAAGGGAAAAACCAGTTCCAGACGTTATATGTTAATA331.5144561725562184No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA271.2391005048187242No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.9178522257916476No Hit
TTTAGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.8260670032124828No Hit
TTTAGGGGGGTCAAGTAATTAAGTGCATGTGGTGGATGCCTTGGCAGTCAGAGGCG120.5507113354749885No Hit
TTTAGGGGGTGTCGTTATACATCTTGGACTGCCGAACTTGCCATCAATGTAAATTG110.5048187241854061No Hit
TTTAGGGGGGCGACCCCAGGTCAGGCGGGGTTACCCGCTGAATTTAAGCATATCAA110.5048187241854061No Hit
TTTAGGGGGGTTATTTAGGGCGCATGGTGGATGCCTTGGCATGCTGAGCCGATGAA110.5048187241854061No Hit
TTTAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.4589261128958238No Hit
TTTAGGGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG100.4589261128958238No Hit
TTTAGGGGGCCCATCCACTTCCCATTACCACTGCATTTATATAGTTCTTTTTCTAA100.4589261128958238No Hit
TTTAGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.4589261128958238No Hit
TTTAGGGATCAATTGAAAGTGGATCAACATGAGAAATTCTGAAAATGGCAAAGGAA100.4589261128958238No Hit
TTTAGGGGGAAGTTGTTACGGGCACACGGTGGATGCCTTGGCATCAGGAGCTGATG90.4130335016062414No Hit
TTTAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.4130335016062414No Hit
TTTAGGGGGAAGCAGTGGTATCAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTT90.4130335016062414No Hit
TTTAGGGGGAGCTGGTTCTCCCCGAAATGCATTTAGGTGCAGCGTTGCGTGGTTGT90.4130335016062414No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA90.4130335016062414No Hit
TTTAGGGGGGTTAAGGAACTCGGCAAAATGACCCCGTAACTTCGGGAGAAGGGGTG80.36714089031665903No Hit
TTTAGGGGGGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGT80.36714089031665903No Hit
TTTAGGGGGTTGTGTGGTTCAATTTATTATCGTACCAAAGCAATTCTTTGACTTGC70.32124827902707664No Hit
TTTAGGGGGGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACGAA70.32124827902707664No Hit
TTTAGGGGGCAGTGTATAACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTT70.32124827902707664No Hit
TTTAGGGGGGAGTGTGACATGAATATTGTATTGTCAGATGAAGCCATGACTCAATT70.32124827902707664No Hit
TTTAGGGGGATAGTTATTGGCAGCATCCTCCTTGCCTGTGATGAGCTGCTCAGGGG60.27535566773749426No Hit
TTTAGGGGGGGTCAAGAAAGAAAGCGCAAACGGTGGATGCCTAGGCAATATACGGC60.27535566773749426No Hit
TTTAGGGGGGTCAAGTAATTAATGCATGTGGTGGATGCCTTGGCAGTCAGAGGCGA60.27535566773749426No Hit
TTTAGGGGGGTGAAAAGCCCGCTCGCCGAAAGCCCAAGGTTTCCTACGCAACGTTC60.27535566773749426No Hit
TTTAGGGGGCGTCTCTACTAAAAATACAAAAATTAGCCAGGTGTGGTGGTGCGCGT60.27535566773749426No Hit
TTTAGGGGGGACATACTGTGAATGCCTCAATCTATGTACTCCGCCAAAAAAAAAAA60.27535566773749426No Hit
TTTAGGGGGGGAGTCGATAACCGGACGTTGAGACCAGGATTTCCGAATGGGGAAAC60.27535566773749426No Hit
TTTAGGGGGGGTGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAA60.27535566773749426No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.27535566773749426No Hit
TTTAGGGGGGGGCTGCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAG60.27535566773749426No Hit
TTTAGGGGGGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.27535566773749426No Hit
TTTAGGGGGAAGCATGGAACGCAGAGTCGACTCTTTTTTTTTTTTTTTTTTTTTTT50.2294630564479119No Hit
TTTAGGGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.2294630564479119No Hit
TTTAGGGGGGGCTGGAGAGATGGCTCACCGGTTAAGAGCACTGACTGATCTTCTGA50.2294630564479119No Hit
TTTAGGAGGGAGTGCAGTGCTTTTAGGGGGGTTGGCCATCAAGATCCCCAAAAAAA50.2294630564479119No Hit
TTTAGGGGGGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTG50.2294630564479119No Hit
TTTAGGGGGGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACG50.2294630564479119No Hit
TTTAGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAGAT50.2294630564479119No Hit
TTTAGGGGGATCTTAGTACCCCTAGGAAAAGAAAATAATAATGATTCCCCTAGTAG50.2294630564479119No Hit
TTTAGGGGAGCGTGGACACATCTGTCGGCCATACCACTTCGCGCAACAGATCGCCC50.2294630564479119No Hit
TTTAGGGGGGGTAAAGTGATGTTCGAAATCGCTGGTGTATCTGAAGAGATCGCTCG50.2294630564479119No Hit
TTTAGGGGGCAACGCATGACCAAAGACGAAACCATCCGCTACCACAAGGATCACTT50.2294630564479119No Hit
TTTAGGGGGAGATGATAAATGAGTAGGGCGGGACACGAAAAAAAAAAAAAAAAAAA50.2294630564479119No Hit
TTTAGGGGGCATCACGCAGAGTCGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.18357044515832951No Hit
TTTAGGGGAGCCACCCCTGAGCATTGCCTTCTGAGCTGTTCTGTGGGACCTAGCTG40.18357044515832951No Hit
TTTAGGGGGCGATGGTAGTCGCCGTGCCTACCATGGTGACCACGGGTGACGGGGAA40.18357044515832951No Hit
TTTAGGGGGACTGTACGGGTATAACTAAATTTAATCAGATCTATGTTATTGGCAAT40.18357044515832951No Hit
TTTAGGTGTCACCGTCTTAGCCCTTTGTTTTCCTTACTAGAGTATCTTTCCCGCAC40.18357044515832951No Hit
TTTAGGGGGGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTT40.18357044515832951No Hit
TTTAGGGGGGATGCCTTGGCATGCTGAGCCGATGAAGGACGTGTGAGACTGCGTTA40.18357044515832951No Hit
TTTAGGGGGCTCGGCGAACTCCCTTCGGTCTCCGGTTTCCTGGTTTCACGGTTTCT40.18357044515832951No Hit
TTTAGGGGGCGTTAATAAACAAGGGAAAAACCAGTTCCAGACGTTATATGTTAATA40.18357044515832951No Hit
TTTAGGGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATG40.18357044515832951No Hit
TTTAGGGGGCGGAAGGTTGGTGTGTTAATTCGGAGTGACTCGTCTTTTCCCAAATG40.18357044515832951No Hit
TTTAGGGGGGCTGGTGAGATGGCCCAATGGTTAAGAACACTGACTGTTCTTCCAGA40.18357044515832951No Hit
TTTAGGGGGGATCAGAGTATGTCTTGTGTGTGAGAGAGCCTTACAATCATGTCCAC40.18357044515832951No Hit
TTTAGGGGGAAAAAACCTTACATAAATTAAAGAATGAATACATTTACAGGTGTCGA40.18357044515832951No Hit
TTTAGGGGGGAACTCTCATTCACAATTGCTACAAAGAGAATAAAATACCTAGGAAT40.18357044515832951No Hit
TTTAGGGGGCATTGCCCGGTGAAAGGATAAGCGGCTGAAACGGTGAGTGGTTTCGC40.18357044515832951No Hit
TTTAGGGGGAAGCAATGGTATCAACGCAGATTCGACTTTTTTTTTTTTTTTTTTTT40.18357044515832951No Hit
TTTAGGGGGACAGGCATATTTAGGACATAACTCAAGGCATCTGGCCTCAGGGTTCT40.18357044515832951No Hit
TTTAGGGGGGTGATGCGGACACCATAAGTGGTGGTAGTTAGTGTGCCTGTGCTGTC40.18357044515832951No Hit
TTTAGGGGGCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGAT30.13767783386874713No Hit
TTTAGGGATGTACAGATTTCCATATGTATCTTTCATAAGGTTTTAACGGCATCTAC30.13767783386874713No Hit
TTTAGGGGGGCAGTGGTGGTGCACACCTTTAATCCCAGCACTTGGGAGGCAGAGGC30.13767783386874713No Hit
TTTAGGGGGGACCGATAGCTGCATCTTTGAGAAGGAGTAATCGTTCGGTTTAGGTG30.13767783386874713No Hit
TTTAGGGGGAGGAGAATTGCGTGTACCCGGAAGGCGGAGCTTGCAGTGAGCCGAGA30.13767783386874713No Hit
TTTAGGGGGTCAGACTTGCAAAAAAAATTTAAAAAAAAAAAAAAAAAAAAAAAAAA30.13767783386874713No Hit
TTTAGGGGGGGAGAGGTGGCTCAGTGGTTGAGAGCACTGGCTGCTCTTCTAGAGGG30.13767783386874713No Hit
TTTAGGGGGTTGACCAAAAAGTTTCGGCTTTGAAACCGAAATAGAGAAATAAAATG30.13767783386874713No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAA30.13767783386874713No Hit
TTTAGGGGGGCAGTGCTTTTAGGGGGGAGTGCAGTGCTGCGTCCGGGAAACAAAGG30.13767783386874713No Hit
TTTAGGGCTTTTTCGCAACGGGTTTGCCGTCAGAACGCAGGTGTTGTGAAAACCAC30.13767783386874713No Hit
TTTAGGGGGCGGCGGCCGTAACGATAACGGTCCTAAGGTAGCGAAATTCCTTGTCG30.13767783386874713No Hit
TTTAGGGGGAAGGGATGGCAGATTTAGCATTCACAGACAACACCGCACACGAGGGC30.13767783386874713No Hit
TTTAGGGGGGCGACCCCAGGTCAGGTGGGGCTACCCGCTGAGTTTAAGCATATAAA30.13767783386874713No Hit
TTTAGGGGGGTGTGGCCATAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13767783386874713No Hit
TTTAGGGGGGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTG30.13767783386874713No Hit
TTTAGGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCGGACTGCTCTTCC30.13767783386874713No Hit
TTTAGGGGGATTTTTTAAGGCAGCAACCTTGCTCCCCATCTGATCTTTTGCCGTGT30.13767783386874713No Hit
TTTAGGGGGAAGGTAAAAAGAGGAACAACAAAAAAAGACAACGGGAAAAAATGCAA30.13767783386874713No Hit
TTTAGGGGGGAGGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13767783386874713No Hit
TTTAGGGGGGCGTGGCGGAATAAAATAAATAAAAATAAAAAAAAAAAAAAAAAAAA30.13767783386874713No Hit
TTTAGGGGGGGGAATTCGTGGAGAAAGAAATGGCTCGCCTGGCAGCATTTGATATG30.13767783386874713No Hit
TTTAGGGGGGAGTCCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA30.13767783386874713No Hit
TTTAGGGGGGCCGATCGACAACCTTAGCAAAGATGCAGATCTTCGTTAAGACCCTG30.13767783386874713No Hit
TTTAGGGGGGGTGGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13767783386874713No Hit
TTTAGGGGGCTGTAGGGGAGACTGGAATTCCTGGTGTAGCGTTGGAATGCGCAGAT30.13767783386874713No Hit
TTTAGGGGGGAGTGCAGTGCTTTTAGGGGGGAGTGCAGTGCTTTTAGGGGGAAAAA30.13767783386874713No Hit
TTTAGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13767783386874713No Hit
TTTAGGGGGGTGAACTTGTCGGAATATAGGGGTACCATCCTCTAAGGCTAAGTAAT30.13767783386874713No Hit
TTTAGGGGGCCGTCTGTTGGCCAGTGTGGGGTGGGTCATCTGAAAGCAGCCACTTC30.13767783386874713No Hit
TTTAGGGGGGTGTGGCCATCAAGATCCCCAAAAGGTATCTTTATTGAGGTCTGGGT30.13767783386874713No Hit
TTTAGGGGGACCCTTCAAAAAATTAATGAATCCAGGAGCTGGTTTTTTGAACGGAT30.13767783386874713No Hit
TTTAGGGGGGGAGTCAATAACCGGACGTTGAGACCAGGATTTCCGAATGGGGAAAC30.13767783386874713No Hit
TTTAGGGGGGTGGTATCAAACTAAAAAGAAAAAAAAATGGAAAAAGCTAACATGAA30.13767783386874713No Hit
TTTAGGGGGGGAGTTGATAACCGGACGTTGAGACCAGGATTTCCGAATGGGGAAAC30.13767783386874713No Hit
TTTAGGGGGGTTGTGTACAGTTCATTGCGCGACAAAAAAGTCCAGTTTCTTCATTG30.13767783386874713No Hit
TTTAGGGGGAACTGTAACAGAGAAATTACCATTTTACTTCAAAAGACCCCCTGTTA30.13767783386874713No Hit
TTTAGGGGGGTCCTCATGCATAGATTCATGATATGTTATGAAAAAAAAAAAAAAAA30.13767783386874713No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13767783386874713No Hit
TTTAGGGCCATCCCATCCCTGAGCCAGGATCAAACTCTACGTACTGAGTCGGAGAC30.13767783386874713No Hit
TTTAGGGAGGTGAAAGGATGTAGGCATGTATCTTAGGCAAATCCGGGGTACTCTAT30.13767783386874713No Hit
TTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAA30.13767783386874713No Hit
TTTAGGGGGATCTTGATGGCAGAGGTGGAGGAAACACTCAAGAGGCTTCAGAGCCA30.13767783386874713No Hit
TTTAGGGGGAGTGCAGTGCTTTTAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13767783386874713No Hit
TTTAGGGGGGTTATTTAGGGCGCCAGGTTTCCCCATTCGGACATCCTCGGATCAAC30.13767783386874713No Hit
TTTAGGGGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13767783386874713No Hit
TTTAGGGGGTGTGGCCATCAAGATCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13767783386874713No Hit
TTTAGGGGGGTGTGCCATCAAGATCCCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.13767783386874713No Hit
TTTAGGGGGAAGGTAAAAAGAGGAACAACTGGCTGAACCTTCATACCCTGACTGAG30.13767783386874713No Hit
TTTAGGGGGGGCATAATGATGGCGGCGCTGTCTCCAGCAGAGGCTCAGTGAAATCG30.13767783386874713No Hit
TTTAGGGGGGCTGGGAAAGGGCACACGGAGGATGCCTAGGCGGCGGGAGCCGAAGA30.13767783386874713No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCCA251.4963756E-549.8372124
AGGGGGC251.4963756E-549.837214
GATCCCC251.4963756E-549.8372123
GGGGTGT251.4963756E-549.837217
GGGTGTG251.4963756E-549.837218
CCCCAAA251.4963756E-549.8372126
TCCCCAA251.4963756E-549.8372125
CAAGATC306.5190034E-749.83720820
AGGGGAA150.00743368649.8372084
TCAAGAT306.5190034E-749.83720819
TGGTATC150.00743368649.83720816
AGTGGTA150.00743368649.83720814
GTATCAA150.00743368649.83720818
CATCAAG306.5190034E-749.83720817
AGCAGTG150.00743368649.83720811
GGGGGTG306.5190034E-749.8372086
GCAGTGG150.00743368649.83720812
GGGGGGT352.7812348E-849.8372085
GGGGGCG150.00743368649.8372086
AAGATCC306.5190034E-749.83720821