FastQCFastQC Report
Fri 17 Jun 2016
SRR1526370_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526370_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17953
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTTCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC430.23951428730574278No Hit
CCTTTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA280.15596279173397204No Hit
CCTTTCGGGGATGTTGTCCTGGCTGTTGCCAAAAAAAAACCAAAAAAAAAAAAAAA210.11697209380047903No Hit
CCTTTCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC210.11697209380047903No Hit
CCTTTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG200.11140199409569432No Hit
CCTTTCGGGATGTTGTCCTGGCTGTTGCCAAAAAAAAAAAAACCAAAACAAAAAAA190.1058318943909096No Hit
CCTTTCGGGATGTTGTCCTGGCTGTTGCCAAAAAAAAAACCAAAAAAAAAAAAAAA180.10026179468612488No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGCT150.00784480150.71022439
TTACTTT150.00784480150.71022441
CAACCTG203.7979707E-450.71022441
AGACCCT203.7979707E-450.71022440
GCCAACC150.00784480150.71022439
TTTGCTT150.00784480150.71022440
CGACCCT150.00784480150.71022440
CCGGAGA150.00811358650.2816936
CCAACAC451.1459633E-1050.2816943
CGGAGAC150.00811358650.2816937
TTTTCAT150.00811358650.2816936
TTTTTCA150.00811358650.2816935
CAACACA402.3155735E-950.2816944
TACTTTT150.00811358650.2816942
CGATCCT150.00811358650.2816936
GTGCAAT505.456968E-1250.049
GCGTGCA550.050.047
GCCAGGA252.0052737E-550.015
CGTGCAA505.456968E-1250.048
CTTTTGA150.00829654249.99999630