##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526370_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17953 Sequences flagged as poor quality 0 Sequence length 56 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.586642900907925 39.0 38.0 40.0 36.0 40.0 2 38.332367849384504 39.0 38.0 40.0 35.0 40.0 3 38.01971815295494 39.0 38.0 40.0 35.0 40.0 4 38.2495961677714 39.0 38.0 40.0 35.0 40.0 5 38.30162089901409 39.0 38.0 40.0 35.0 40.0 6 37.85812956051913 39.0 38.0 40.0 35.0 40.0 7 37.993650086336544 39.0 38.0 40.0 35.0 40.0 8 37.88792959393973 39.0 38.0 40.0 35.0 40.0 9 37.99025232551663 39.0 38.0 40.0 35.0 40.0 10 37.4360831058876 39.0 38.0 40.0 34.0 40.0 11 35.601904974099035 38.0 35.0 39.0 29.0 40.0 12 33.387623238455966 38.0 31.0 39.0 19.0 40.0 13 32.851668244861585 37.0 30.0 39.0 17.0 40.0 14 32.38756753745892 37.0 29.0 39.0 15.0 40.0 15 32.99014092352253 37.0 31.0 39.0 18.0 40.0 16 32.61142984459422 36.0 30.0 39.0 18.0 40.0 17 32.680387678939454 36.0 30.0 39.0 18.0 40.0 18 32.73514175903749 36.0 30.0 39.0 19.0 40.0 19 33.821144098479365 36.0 31.0 39.0 24.0 40.0 20 33.82972205202473 36.0 31.0 39.0 24.0 40.0 21 35.439982175680946 38.0 35.0 39.0 27.0 40.0 22 34.96596669080377 38.0 34.0 39.0 26.0 40.0 23 35.26664067286804 38.0 35.0 39.0 27.0 40.0 24 34.80883417813179 38.0 34.0 39.0 25.0 40.0 25 35.20486826714198 38.0 35.0 39.0 27.0 40.0 26 35.59950983122598 38.0 35.0 39.0 29.0 40.0 27 35.58569598395811 38.0 35.0 39.0 29.0 40.0 28 35.54208210326965 38.0 35.0 39.0 29.0 40.0 29 35.419038600790955 38.0 35.0 39.0 28.0 40.0 30 35.242132234166995 38.0 35.0 39.0 28.0 40.0 31 34.92079318219796 38.0 35.0 39.0 27.0 40.0 32 33.92859132178466 38.0 33.0 39.0 20.0 40.0 33 35.14320726341001 38.0 35.0 39.0 28.0 40.0 34 34.91878794630424 38.0 35.0 39.0 27.0 40.0 35 34.99838467108561 38.0 35.0 39.0 28.0 40.0 36 32.724001559627915 35.0 31.0 38.0 24.0 39.0 37 32.44555227538573 35.0 31.0 38.0 23.0 39.0 38 31.788002005235892 35.0 31.0 38.0 18.0 39.0 39 29.773798250988694 34.0 28.0 38.0 6.0 39.0 40 25.59483094747396 31.0 12.0 37.0 4.0 39.0 41 22.04534061159695 26.0 10.0 32.0 4.0 36.0 42 26.09491449896953 30.0 22.0 34.0 4.0 37.0 43 21.70634434356375 26.0 8.0 33.0 4.0 37.0 44 22.819528769564975 28.0 9.0 33.0 4.0 37.0 45 25.643290814905587 30.0 21.0 34.0 4.0 37.0 46 25.679329359995545 30.0 17.0 36.0 4.0 38.0 47 24.818804656603355 30.0 11.0 36.0 4.0 38.0 48 24.81139642399599 30.0 12.0 35.0 4.0 38.0 49 21.889099314877736 26.0 5.0 35.0 4.0 38.0 50 24.61755695426948 30.0 10.0 35.0 4.0 38.0 51 26.09703113685735 32.0 4.0 38.0 4.0 40.0 52 25.78421433743664 32.0 4.0 38.0 4.0 40.0 53 25.334818693254608 31.0 4.0 38.0 4.0 40.0 54 24.46777697320782 31.0 4.0 38.0 4.0 40.0 55 24.76037431070016 31.0 4.0 38.0 4.0 40.0 56 24.2819584470562 31.0 4.0 38.0 4.0 40.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.009995761612003662 1 2 0.4854085750302204 1 3 0.8348528808369267 1 4 0.5002075684105094 1 5 0.2723137092549024 1 6 0.8008423847990827 1 7 0.43730287361034215 1 8 0.6989364052404881 1 9 0.5693585364652236 1 10 0.4431308530139191 1 11 -5.079164474682475 1 12 -7.493423884592339 1 13 -21.277181128658608 1 14 -16.94224542118271 1 15 -10.235801850833575 1 16 -22.20744589917953 1 17 -21.951546323283843 1 18 -19.882758163602958 1 19 -7.4132800004240025 1 20 -10.502719752134539 1 21 -9.218974799878882 1 22 -2.939881954489973 1 23 -3.189331139848761 1 24 -3.2033926311639185 1 25 -3.642072565861831 1 26 2.223808450448793 1 27 1.6903578529180123 1 28 0.3517925655957015 1 29 1.61980587656236 1 30 0.7865117516884865 1 31 -0.5821209369682165 1 32 -11.993768942169584 1 33 0.05596728851642041 1 34 0.14435409171731095 1 35 -0.5273958912098919 1 36 3.102129710407585 1 37 3.716655162498146 1 38 4.026510264241054 1 39 7.152834578435165 1 40 9.724221193981595 1 41 -1.288474258818006 1 42 -5.195809355684034 1 43 -12.409577173630973 1 44 -15.808664410752549 1 45 -12.880371490926809 1 46 -18.76338913020924 1 47 -17.459817361620175 1 48 -15.368547014074753 1 49 -12.868116031174614 1 50 -15.233631639427198 1 51 -15.75910331752567 1 52 -15.367497740303335 1 53 -15.534461035928496 1 54 -17.523725961374147 1 55 -14.925676166158134 1 56 -13.988605473675346 2 1 -0.43545878384254166 2 2 0.039954029575675065 2 3 0.5893983353823771 2 4 0.05475302295596407 2 5 -0.07314083619964151 2 6 0.5053878393445359 2 7 0.10093923724670617 2 8 0.35348185978594415 2 9 -0.04882328171659367 2 10 0.6976763075593695 2 11 -2.583709929227929 2 12 -13.038878430046886 2 13 -11.577181128658609 2 14 -15.369518148455438 2 15 -9.417620032651758 2 16 -3.8029004446340764 2 17 -2.22881905055657 2 18 -10.550939981784776 2 19 -2.8223709095149125 2 20 -3.948174297589084 2 21 0.09466156375748369 2 22 1.4964816818736608 2 23 1.1833961328785136 2 24 0.9602437324724491 2 25 1.048836525047264 2 26 0.4283539049942462 2 27 -0.4687330561728942 2 28 0.26997438377752303 2 29 0.7970786038350894 2 30 1.0183299335066636 2 31 -5.2548482096954885 2 32 -10.361950760351402 2 33 -4.971305438756303 2 34 -4.992009544646326 2 35 -4.477395891209895 2 36 3.6430388013166812 2 37 3.9621097079526955 2 38 4.921964809695602 2 39 6.09828912388971 2 40 8.810584830345231 2 41 7.911525741181993 2 42 3.0769179170432395 2 43 -1.0595771736309736 2 44 -8.308664410752549 2 45 3.5514466908913747 2 46 -12.240661857481967 2 47 -11.71436281616563 2 48 -9.25491065043839 2 49 -10.427206940265522 2 50 -9.20635891215447 2 51 -9.927285135707487 2 52 -9.453861376666971 2 53 -10.434461035928496 2 54 -15.787362325010513 2 55 -9.77113071161268 2 56 -10.352241837311709 3 1 -0.1486249280431693 3 2 0.8095465060647058 3 3 1.462439087733479 3 4 0.7208972235829236 3 5 0.6964516402893892 3 6 0.9991182468680506 3 7 0.754544252920681 3 8 0.7092812328266973 3 9 0.7176343985341944 3 10 1.1224411978415034 3 11 0.7501458701451114 3 12 0.6962312878214547 3 13 -4.335801818313779 3 14 -8.176728179803401 3 15 -4.322008747385297 3 16 3.3977265146135736 3 17 2.2225916077506405 3 18 1.8534487329487632 3 19 1.3280993099208231 3 20 0.924866454762018 3 21 2.955163131155601 3 22 3.44632494206175 3 23 3.2382550670477954 3 24 3.8552280584912566 3 25 3.0096515720692025 3 26 2.4565670711384513 3 27 2.6989785425731867 3 28 2.33110291042329 3 29 1.6215300144933948 3 30 2.5778910620333093 3 31 3.569603200962817 3 32 2.507955195761447 3 33 2.928381081619868 3 34 3.247802367579382 3 35 2.712259281203899 3 36 5.034888331097246 3 37 5.640793093532633 3 38 6.566165436654849 3 39 9.93214492326275 3 40 10.863876366395392 3 41 10.411525741181993 3 42 8.735225127074585 3 43 0.6473193780931652 3 44 4.880990761661245 3 45 7.093766440107675 3 46 0.831438455997656 3 47 -3.8977483961029336 3 48 0.1400736755804175 3 49 -9.656047065657372 3 50 0.08360973988314768 3 51 0.5633104755777794 3 52 0.13077812176562986 3 53 -0.8654955186871156 3 54 -3.3030363062017365 3 55 -1.0877451316753763 3 56 1.5976014228763766 4 1 -0.3577461738718668 4 2 0.21444083309474138 4 3 0.6025948163207957 4 4 0.22923982647503038 4 5 0.0045717737710333495 4 6 0.09922948157327482 4 7 -0.05301970703482084 4 8 -0.2139668205659575 4 9 0.20484240743297022 4 10 0.09796956269133261 4 11 2.822448428543332 4 12 3.683995470246373 4 13 4.648625322954295 4 14 1.6964642562366414 4 15 5.006133633037393 4 16 5.7748121653365985 4 17 5.530711741232285 4 18 4.751112804138977 4 19 4.022203870543741 4 20 3.935989925284815 4 21 2.231025200121117 4 22 2.8278599809938996 4 23 1.9267978924093043 4 24 3.6127364010941463 4 25 2.361153240589786 4 26 2.489937482706857 4 27 1.7145514013051084 4 28 0.8227603075311833 4 29 1.5214187797881706 4 30 2.142963364591708 4 31 2.759814546902753 4 32 3.1497794449271908 4 33 2.949515675613199 4 34 2.660483123975375 4 35 2.700023463628817 4 36 4.9714845491172674 4 37 5.142461614111053 4 38 4.102316715853959 4 39 7.714124901015811 4 40 9.819382484304182 4 41 0.41152574118199325 4 42 6.417093870122418 4 43 5.892035729594834 4 44 9.159077524731323 4 45 6.821241412298999 4 46 5.228546353661727 4 47 4.336956831928212 4 48 4.736291695602667 4 49 4.06252913011571 4 50 5.211529650895383 4 51 5.552187005054975 4 52 6.1986312919547295 4 53 5.517151867297311 4 54 0.49562887733552685 4 55 7.00013028545477 4 56 5.624297752131106 5 1 0.2638419154581584 5 2 0.9315624211840685 5 3 0.250237496221537 5 4 0.9078998761028174 5 5 0.5107752477164453 5 6 0.43545776941446945 5 7 0.5065336428411058 5 8 0.8220133283174107 5 9 0.538589305695993 5 10 1.0123616222446827 5 11 2.370835525317524 5 12 5.852729961561508 5 13 5.615126563649085 5 14 5.242369963432676 5 15 6.033428918397192 5 16 6.127169485435857 5 17 5.267684445946923 5 18 4.805703374858581 5 19 4.932873845729844 5 20 3.7895879401731563 5 21 2.807948277044197 5 22 2.437041122433108 5 23 0.7183611678435469 5 24 3.3273765996053157 5 25 1.9963889725997106 5 26 1.012269988910333 5 27 1.9018963144564722 5 28 0.5671771809803161 5 29 1.0313443381008227 5 30 2.8365117516884837 5 31 1.556340601493325 5 32 3.7254618270611815 5 33 3.179044211593343 5 34 2.5289694763326978 5 35 3.0610656472516453 5 36 4.898283556561434 5 37 5.532039777882762 5 38 5.299587187317975 5 39 8.668219193819777 5 40 4.481913501673905 5 41 5.7961411257973765 5 42 8.304190644315966 5 43 12.748115134061333 5 44 11.806720204632065 5 45 8.985013124457804 5 46 9.448149331329219 5 47 10.124798022995211 5 48 9.881452985925247 5 49 10.712653199594616 5 50 9.027906822111262 5 51 8.390896682474331 5 52 8.297886875081282 5 53 9.104000502533044 5 54 8.11473557708739 5 55 9.851246910764946 5 56 9.857548372478501 6 1 1.2499957616120057 6 2 1.7254085750302224 6 3 2.154852880836927 6 4 1.620207568410514 6 5 1.3323137092549047 6 6 2.110842384799085 6 7 1.7973028736103416 6 8 1.8389364052404886 6 9 1.5493585364652276 6 10 0.9031308530139128 6 11 4.410835525317523 6 12 7.04657611540766 6 13 7.302818871341394 6 14 7.777754578817291 6 15 7.664198149166424 6 16 8.182554100820468 6 17 7.778453676716158 6 18 7.027241836397039 6 19 6.046719999575998 6 20 5.617280247865466 6 21 3.6310252001211154 6 22 4.300118045510025 6 23 3.9706688601512425 6 24 4.736607368836083 6 25 3.4979274341381696 6 26 3.3738084504487915 6 27 1.5003578529180146 6 28 3.471792565595706 6 29 3.5498058765623597 6 30 3.456511751688481 6 31 4.497879063031782 6 32 3.196231057830417 6 33 4.395967288516424 6 34 4.944354091717308 6 35 4.942604108790107 6 36 4.352129710407585 6 37 4.396655162498146 6 38 6.556510264241055 6 39 8.152834578435165 6 40 4.174221193981598 6 41 1.0115257411819947 6 42 7.644190644315966 6 43 13.200422826369024 6 44 11.23133558924745 6 45 8.40962850907319 6 46 10.966610869790756 6 47 12.280182638379824 6 48 12.341452985925248 6 49 11.521883968825385 6 50 11.6863683605728 6 51 9.950896682474333 6 52 9.722502259696665 6 53 9.745538964071503 6 54 10.036274038625852 6 55 9.454323833841865 6 56 10.011394526324654 7 1 0.9578218485685284 7 2 1.1288868358997846 7 3 0.9826789677934471 7 4 0.8393380031931201 7 5 0.9723137092549052 7 6 1.50736412392952 7 7 1.2373028736103393 7 8 1.2685016226317956 7 9 1.5302281016826171 7 10 0.9170438964921743 7 11 3.849096394882743 7 12 5.5283152458424425 7 13 6.335862349602259 7 14 6.683841535339031 7 15 6.568545975253379 7 16 6.177336709516119 7 17 6.585410198455289 7 18 6.223763575527478 7 19 5.043241738706435 7 20 5.01032372612633 7 21 3.404938243599375 7 22 3.6427267411621997 7 23 3.3671905992816775 7 24 3.6618247601404335 7 25 3.153579608051217 7 26 3.1042432330574883 7 27 1.983836113787575 7 28 2.560488217769617 7 29 2.635023267866714 7 30 3.640859577775437 7 31 3.6700529760752616 7 32 4.217100623047806 7 33 1.8864020711251186 7 34 3.100875830847748 7 35 3.239995413137933 7 36 2.726042753885846 7 37 3.829698640759016 7 38 4.441727655545407 7 39 6.683269361043859 7 40 1.174221193981598 7 41 -10.501517737078876 7 42 5.543321079098575 7 43 10.223031522021202 7 44 8.756552980551799 7 45 5.282671987334062 7 46 8.225741304573368 7 47 9.501052203597215 7 48 8.381452985925247 7 49 9.873188316651472 7 50 8.522890099703236 7 51 9.173505378126503 7 52 10.595545737957533 7 53 10.891625920593246 7 54 9.424100125582374 7 55 9.948236877320124 7 56 9.924438004585525 8 1 0.5299957616120068 8 2 0.8054085750302207 8 3 1.0348528808369224 8 4 0.9402075684105142 8 5 0.6923137092549041 8 6 1.5508423847990827 8 7 1.4373028736103421 8 8 1.5589364052404875 8 9 1.0693585364652236 8 10 1.9831308530139182 8 11 2.9308355253175264 8 12 6.166576115407658 8 13 6.062818871341392 8 14 7.257754578817288 8 15 5.904198149166426 8 16 7.10255410082047 8 17 6.418453676716158 8 18 5.587241836397041 8 19 4.606719999576001 8 20 5.097280247865463 8 21 3.8310252001211182 8 22 4.42011804551003 8 23 4.130668860151239 8 24 3.6566073688360845 8 25 4.417927434138171 8 26 1.973808450448793 8 27 1.9003578529180132 8 28 2.031792565595701 8 29 1.349805876562364 8 30 1.6965117516884831 8 31 2.3778790630317843 8 32 3.3962310578304127 8 33 2.0359672885164244 8 34 3.18435409171731 8 35 1.742604108790104 8 36 0.9921297104075855 8 37 1.6766551624981467 8 38 2.236510264241055 8 39 4.672834578435161 8 40 -0.4657788060184025 8 41 -8.588474258818007 8 42 6.204190644315965 8 43 10.840422826369025 8 44 10.111335589247453 8 45 5.8096285090731925 8 46 7.366610869790758 8 47 7.560182638379825 8 48 8.421452985925246 8 49 11.121883968825387 8 50 8.326368360572804 8 51 9.910896682474334 8 52 9.802502259696663 8 53 9.425538964071503 8 54 10.47627403862585 8 55 8.654323833841865 8 56 9.611394526324656 9 1 0.9578218485685284 9 2 1.433234661986745 9 3 1.1565920112717052 9 4 1.274120611888769 9 5 1.2766615353418587 9 6 1.9856249934947314 9 7 1.280781134479902 9 8 1.572849448718749 9 9 1.7041411451608752 9 10 1.786609113883479 9 11 4.457792047056657 9 12 6.74570655019027 9 13 6.422818871341391 9 14 7.205580665773812 9 15 6.785937279601207 9 16 7.177336709516119 9 17 6.368018894107461 9 18 6.832459227701392 9 19 5.86932869522817 9 20 4.9668454652567675 9 21 3.970155634903726 9 22 4.68620500203177 9 23 2.0193645123251542 9 24 4.574868238401301 9 25 3.4144491732686078 9 26 1.3216345374053162 9 27 2.7229665485701844 9 28 2.2561403916826563 9 29 2.98284935482323 9 30 3.901729142992835 9 31 4.843966019553527 9 32 5.956231057830415 9 33 3.582054245038165 9 34 3.2313106134564435 9 35 3.283473674007496 9 36 2.856477536494541 9 37 2.655785597280758 9 38 3.74607548163236 9 39 3.900660665391687 9 40 -4.521430979931445 9 41 -4.153691650122354 9 42 6.238973253011618 9 43 12.005640217673374 9 44 9.365248632725713 9 45 7.543541552551453 9 46 10.182263043703806 9 47 10.240182638379824 9 48 10.99014863809916 9 49 13.612318751434081 9 50 9.74028140405106 9 51 8.13002711725694 9 52 7.030328346653189 9 53 7.761191137984547 9 54 7.293665342973675 9 55 7.122149920798389 9 56 4.576611917629002 10 1 0.6516624282786694 10 2 0.4187419083635575 10 3 1.7181862141702595 10 4 0.7668742350771751 10 5 0.8889803759215695 10 6 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-4.6437689421695865 114 33 0.3559672885164247 114 34 0.7443540917173124 114 35 1.0892707754567752 114 36 3.6187963770742577 114 37 3.783321829164816 114 38 3.9431769309077254 114 39 7.752834578435166 114 40 9.840887860648262 114 41 8.211525741181994 114 42 3.1375239776492982 114 43 3.1737561597023607 114 44 1.0580022559141185 114 45 0.9696285090731926 114 46 -3.71338913020924 114 47 -6.026484028286841 114 48 -4.9518803474080855 114 49 -12.251449364507948 114 50 -2.4336316394271975 114 51 -0.2757699841923369 114 52 1.0158355930299976 114 53 -0.26779436926182854 114 54 -0.3903926280408143 114 55 -0.02567616615813506 114 56 4.611394526324656 115 1 -1.015004238387995 115 2 -0.4145914249697782 115 3 0.07235288083692382 115 4 -0.3997924315894892 115 5 -1.0901862907450948 115 6 0.11334238479908265 115 7 -0.5751971263896607 115 8 -0.0385635947595091 115 9 -0.29314146353477355 115 10 0.18063085301391624 115 11 -0.12916447468247583 115 12 -4.055923884592339 115 13 -0.8896811286586086 115 14 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52 -3.5049977403033346 115 53 -3.9969610359284964 115 54 -3.3612259613741493 115 55 -3.5381761661581344 115 56 -1.1136054736753458 116 1 0.23499576161200508 116 2 0.7104085750302218 116 3 1.2598528808369238 116 4 0.7252075684105108 116 5 0.5973137092549052 116 6 0.48834238479908265 116 7 0.8623028736103393 116 8 0.8364364052404909 116 9 0.8943585364652265 116 10 0.9931308530139162 116 11 -0.8791644746824758 116 12 -2.055923884592339 116 13 -6.389681128658609 116 14 -10.26724542118271 116 15 -3.4233018508335746 116 16 -9.63244589917953 116 17 -12.564046323283844 116 18 -4.045258163602959 116 19 -3.2257800004240025 116 20 -4.090219752134537 116 21 2.106025200121117 116 22 1.6101180455100277 116 23 1.5981688601512403 116 24 2.1591073688360822 116 25 1.2704274341381705 116 26 1.661308450448793 116 27 1.3153578529180123 116 28 1.514292565595703 116 29 0.1323058765623628 116 30 0.9615117516884837 116 31 1.8928790630317849 116 32 -3.168768942169585 116 33 2.3184672885164233 116 34 1.831854091717311 116 35 2.647604108790105 116 36 5.302129710407588 116 37 5.041655162498149 116 38 4.501510264241055 116 39 7.240334578435164 116 40 9.236721193981598 116 41 4.474025741181993 116 42 0.9916906443159661 116 43 -7.122077173630974 116 44 -3.6211644107525487 116 45 3.4321285090731912 116 46 -7.638389130209241 116 47 -15.259817361620176 116 48 -9.306047014074753 116 49 -8.705616031174614 116 50 -6.1836316394271975 116 51 -2.234103317525669 116 52 -2.7549977403033346 116 53 -5.309461035928496 116 54 -4.736225961374149 116 55 -4.600676166158134 116 56 0.011394526324654208 117 1 -1.5718224202061748 117 2 -1.8236823340606847 117 3 -1.1833289373448963 117 4 -0.9907015224985827 117 5 -0.9367771998360013 117 6 -0.7218848879281907 117 7 -1.035424399116934 117 8 -1.2374272311231422 117 9 -1.1851869180802268 117 10 -1.6205055106224506 117 11 -11.99280083831884 117 12 -12.357060248228702 117 13 -15.577181128658609 117 14 -13.596790875728164 117 15 -9.917620032651758 117 16 -12.893809535543166 117 17 -6.13790995964748 117 18 -6.369121799966596 117 19 -4.367825454969456 117 20 -3.902719752134537 117 21 0.049207018302936945 117 22 -0.3217001363081522 117 23 0.5015779510603338 117 24 -1.8124835402548243 117 25 -0.9057089294981964 117 26 1.5192629959033397 117 27 0.03126694382710582 117 28 -0.32093470713157046 117 29 -0.02110321434673068 117 30 0.5183299335066636 117 31 0.01787906303178488 117 32 -10.407405305805948 117 33 -0.42585089330175663 117 34 0.8716268189900376 117 35 0.2044222906082851 117 36 2.7339478922257676 117 37 1.0984733443163286 117 38 3.0583284460592353 117 39 6.09828912388971 117 40 6.174221193981598 117 41 3.95698028663654 117 42 1.3496451897705128 117 43 -5.014122628176429 117 44 -2.3541189562070954 117 45 1.915083054527738 117 46 -5.1497527665728775 117 47 -9.396180997983812 117 48 -6.25491065043839 117 49 -4.972661485720069 117 50 -3.160904366699924 117 51 -3.154557862980216 117 52 -2.044770467576061 117 53 -4.8435519450194064 117 54 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118 36 1.4807011389790183 118 37 0.8452265910695758 118 38 2.805081692812486 118 39 1.4814060070065906 118 40 6.031364051124456 118 41 2.982954312610566 118 42 0.0899049300302508 118 43 -2.9167200307738312 118 44 -5.88009298218112 118 45 -3.9875143480696664 118 46 -9.870531987352098 118 47 -16.116960218763033 118 48 -11.40426129978904 118 49 -11.160973174031756 118 50 -8.790774496570055 118 51 -9.751960460382811 118 52 -9.603212026017621 118 53 -10.14874675021421 118 54 -13.92372596137415 118 55 -10.797104737586706 118 56 -8.274319759389632 119 1 -0.747147095530849 119 2 -0.2717342821126323 119 3 0.2777100236940697 119 4 -0.2569352887323433 119 5 -0.38482914788794886 119 6 -0.09201475805806325 119 7 -0.4055542692468066 119 8 -0.243920737616655 119 9 -0.44492717782048885 119 10 -0.6854405755575144 119 11 -8.843450188968191 119 12 -10.350566741735197 119 13 -2.0057525572300356 119 14 -0.7851025640398532 119 15 -7.664373279405002 119 16 -10.686017327750957 119 17 -10.572974894712416 119 18 -1.8756153064601016 119 19 -1.8418514289954295 119 20 -1.3312911807059642 119 21 -1.2689747998788832 119 22 -2.2827390973471147 119 23 -2.5893311398487597 119 24 -8.689106916878202 119 25 -2.5420725658618295 119 26 1.0452370218773623 119 27 0.8689292814894429 119 28 -1.548207434404297 119 29 0.3555201622766475 119 30 1.1936546088313378 119 31 0.08930763446035428 119 32 -10.543768942169585 119 33 -0.029746997197861447 119 34 -0.21278876542554315 119 35 0.3797469659329593 119 36 4.337843996121872 119 37 4.416655162498149 119 38 5.16222454995534 119 39 7.909977435578018 119 40 10.245649765410167 119 41 7.911525741181993 119 42 3.0184763586016814 119 43 -3.202434316488116 119 44 -3.7372358393239757 119 45 0.4410570805017642 119 46 -3.941960558780668 119 47 -9.188388790191604 119 48 -8.689975585503324 119 49 -5.8038303168889005 119 50 -3.790774496570055 119 51 -3.609103317525669 119 52 -4.03178345458905 119 53 -9.577318178785639 119 54 -11.352297389945578 119 55 -9.868533309015277 119 56 -7.988605473675346 120 1 -0.6976965460803015 120 2 -0.2222837326620848 120 3 0.09639134237538372 120 4 -0.6690232008202557 120 5 -0.33537859843740137 120 6 0.16622700018369585 120 7 -0.1473125110050475 120 8 -0.3702943639902756 120 9 -1.1921799250732334 120 10 -0.02609991621685026 120 11 -5.7060875516055525 120 12 -2.224193115361569 120 13 -6.423334974812455 120 14 -2.4499377288750175 120 15 -10.966571081602805 120 16 -10.71898436071799 120 17 -7.040007861745384 120 18 -1.7327581636029592 120 19 -2.067126154270156 120 20 -1.8257966752114605 120 21 -0.4612824921865766 120 22 0.6293488147407942 120 23 -0.743177293694913 120 24 -7.364931092702378 120 25 -0.9266879504772163 120 26 2.2045776812180264 120 27 2.4018963144564722 120 28 1.951792565595703 120 29 2.223652030408516 120 30 2.028819443996177 120 31 -0.21289016773744862 120 32 -7.505307403708045 120 33 0.14058267313181005 120 34 -5.394107446744229 120 35 1.2533733395593387 120 36 3.090591248869128 120 37 3.9166551624981487 120 38 3.9534333411641356 120 39 4.16821919381978 120 40 -3.9796249598645552 120 41 3.873064202720453 120 42 -1.734270894145574 120 43 0.748115134061333 120 44 -1.962510564598702 120 45 -10.3226791832345 120 46 -12.590312207132317 120 47 -10.37520197700479 120 48 -8.310854706382445 120 49 -14.287346800405384 120 50 -7.741323947119506 120 51 -11.609103317525669 120 52 -10.086728509534105 120 53 -10.472922574390035 120 54 -10.15449519214338 120 55 -10.302599243081211 120 56 -8.065528550598422 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 31.0 5 9.0 6 22.0 7 21.0 8 40.0 9 53.0 10 48.0 11 34.0 12 36.0 13 23.0 14 26.0 15 43.0 16 78.0 17 68.0 18 137.0 19 147.0 20 177.0 21 231.0 22 339.0 23 427.0 24 480.0 25 539.0 26 527.0 27 601.0 28 655.0 29 691.0 30 757.0 31 872.0 32 1015.0 33 1239.0 34 1529.0 35 1739.0 36 2008.0 37 1870.0 38 1060.0 39 381.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 0.0 0.0 0.0 100.0 7 99.0865036484153 0.607140867821534 0.19495348966746506 0.11140199409569432 8 99.13663454575837 0.5848604690023952 0.1503926920291873 0.12811229321004847 9 97.5596166703811 1.1979050590595053 0.5237352351236907 0.7187430354357032 10 47.033921907202135 32.32328858686571 8.778477134740712 11.864312371191446 11 27.766947028351808 30.496295883696316 20.82103269648527 20.915724391466608 12 33.225644739040824 14.721773519746003 23.071352977218293 28.981228763994878 13 24.720102489834566 13.262407397092407 27.683395532780036 34.33409458029299 14 17.811577246643267 20.11811242966182 29.22168365925678 32.84862666443813 15 23.171344083021815 20.052446577023936 25.76577581878034 31.01043352117391 16 22.917479244441967 20.215077728868337 28.199699114058056 28.66774391263164 17 23.096206339479693 25.291070135368503 22.750821681243387 28.861901843908417 18 23.928909688562037 29.193826954147863 20.970527605994764 25.906735751295333 19 23.207620745362377 29.33541306890981 20.35541195476575 27.101554230962066 20 25.52515740792333 26.93486376553184 22.45500640775617 25.084972418788652 21 27.871866295264624 25.197771587743734 21.214484679665738 25.715877437325908 22 25.756141034924525 26.0569264189829 21.51729515958336 26.66963738650922 23 25.466495850275724 27.505152342226925 25.97337492341113 21.054976884086223 24 22.58118420319724 28.507770289088175 23.67292374533504 25.238121762379546 25 23.333147663343173 29.198462652481478 24.02384002673648 23.444549657438866 26 21.09139604954804 29.756723579957594 29.2433880147305 19.908492355763865 27 22.833110516934045 23.651960784313726 28.737745098039213 24.777183600713013 28 20.583477436269316 25.73771406258716 30.11100574552351 23.567802755620015 29 18.366209740332106 24.919202050596233 31.639362532040565 25.075225677031092 30 17.416012034096607 28.859546492840828 32.93776812078668 20.78667335227589 31 17.436927885688768 30.162982808662647 34.723152489394955 17.676936816253626 32 19.993315135647038 25.235362932427165 34.11509108127681 20.65623085064899 33 18.794642857142858 27.650669642857146 36.356026785714285 17.198660714285715 34 18.24516416745638 28.30146607949161 38.240704610067446 15.212665142984557 35 19.61767820319902 28.841330881123557 38.404949005183084 13.136041910494342 36 17.148276246792367 28.383353787794263 37.52091933504407 16.947450630369296 37 16.989559488582433 28.323376695885212 37.725420132879236 16.96164368265312 38 17.891391614629796 31.662578055307762 37.834522747546835 12.61150758251561 39 17.213206491326243 24.907666480134303 33.670956911024064 24.20817011751539 40 10.867492850333651 19.985420288229687 26.97807435653003 42.16901250490663 41 13.484030589293747 22.17161493477283 29.104813315339634 35.23954116059379 42 15.278165280955303 31.722560124993027 38.870598738909656 14.128675855142012 43 10.596322584250824 16.78868831386576 29.721119991057954 42.89386911082546 44 10.9717868338558 19.31258396775638 26.780116435288846 42.93551276309897 45 15.137998327293003 28.709227766936156 40.49066071926401 15.66211318650683 46 16.146471965221266 28.948835135436408 39.917511983056514 14.98718091628581 47 14.744232698094283 27.788922322523124 34.832274601582526 22.634570377800067 48 15.634053933585914 31.028526855359928 37.37463784265656 15.962781368397593 49 10.612746467072558 19.935206389990505 26.705021504775733 42.747025638161205 50 13.894313933374253 31.968082138273534 39.86942692930082 14.268176999051393 51 17.531015983011063 31.278640885212923 36.93416787750084 14.256175254275178 52 15.047810770005032 33.42280378012637 35.20102891013812 16.32835653973047 53 14.963442540603896 34.151922754925494 36.02165541106212 14.862979293408497 54 14.561374156488762 28.765824549662593 32.60275500529809 24.070046288550557 55 15.834402722758467 31.78039390726999 37.98471238073983 14.400490989231713 56 16.5234375 31.874999999999996 35.27901785714286 16.322544642857142 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 1.0 9 2.0 10 1.0 11 0.0 12 5.0 13 39.0 14 68.0 15 119.0 16 170.0 17 209.0 18 248.0 19 266.5 20 285.0 21 295.0 22 337.0 23 369.0 24 410.5 25 452.0 26 535.5 27 619.0 28 663.0 29 765.0 30 823.0 31 878.0 32 933.0 33 919.5 34 906.0 35 854.0 36 802.0 37 676.5 38 482.0 39 413.0 40 386.0 41 359.0 42 341.0 43 323.0 44 304.5 45 286.0 46 288.0 47 315.5 48 341.0 49 388.0 50 435.0 51 449.0 52 463.0 53 492.5 54 551.5 55 581.0 56 615.5 57 650.0 58 709.5 59 769.0 60 826.5 61 884.0 62 878.5 63 864.5 64 856.0 65 835.5 66 815.0 67 702.0 68 589.0 69 507.5 70 426.0 71 377.0 72 251.0 73 174.0 74 164.0 75 154.0 76 123.0 77 92.0 78 70.0 79 34.0 80 20.0 81 15.5 82 11.0 83 5.5 84 0.0 85 0.5 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.02785049852392358 10 0.0 11 0.0 12 0.0 13 0.0 14 0.02228039881913886 15 0.16710299114354146 16 0.0334205982287083 17 0.01114019940956943 18 0.02228039881913886 19 0.01114019940956943 20 0.0334205982287083 21 0.01671029911435415 22 0.0 23 0.0 24 0.0 25 0.0 26 0.1726730908483262 27 0.005570099704784715 28 0.1448225923244026 29 0.03899069793349301 30 0.02228039881913886 31 0.2060936890770345 32 0.01114019940956943 33 0.1838132902578956 34 0.07798139586698602 35 0.05570099704784716 36 0.1503926920291873 37 0.23394418760095806 38 0.09469169498134017 39 0.4623182754971314 40 0.6684119645741659 41 0.9413468501086171 42 0.1782431905531109 43 0.3342059822870829 44 0.4957388737258397 45 0.10026179468612488 46 0.06127109675263188 47 0.03899069793349301 48 0.02785049852392358 49 0.2785049852392358 50 0.1782431905531109 51 0.3286358825822982 52 0.3899069793349301 53 0.20052358937224976 54 0.12254219350526376 55 0.16710299114354146 56 0.1838132902578956 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 17953.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.58096139920904 #Duplication Level Percentage of deduplicated Percentage of total 1 94.39798302791785 85.50660056815018 2 4.0769893002090765 7.385952208544532 3 0.7563645308080187 2.05536679106556 4 0.1967777641126553 0.7129727622124435 5 0.1906284589841348 0.8633654542416309 6 0.1106874923133686 0.6015707681167493 7 0.07379166154224573 0.4678883752019161 8 0.030746525642602385 0.22280398819138864 9 0.043045135899643344 0.3509162814014371 >10 0.12298610257040954 1.8325628028741714 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTTTCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 43 0.23951428730574278 No Hit CCTTTCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 28 0.15596279173397204 No Hit CCTTTCGGGGATGTTGTCCTGGCTGTTGCCAAAAAAAAACCAAAAAAAAAAAAAAA 21 0.11697209380047903 No Hit CCTTTCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC 21 0.11697209380047903 No Hit CCTTTCGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 20 0.11140199409569432 No Hit CCTTTCGGGATGTTGTCCTGGCTGTTGCCAAAAAAAAAAAAACCAAAACAAAAAAA 19 0.1058318943909096 No Hit CCTTTCGGGATGTTGTCCTGGCTGTTGCCAAAAAAAAAACCAAAAAAAAAAAAAAA 18 0.10026179468612488 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.005570099704784716 0.0 0.0 0.0 0.0 12 0.005570099704784716 0.0 0.0 0.0 0.0 13 0.011140199409569432 0.0 0.0 0.0 0.0 14 0.011140199409569432 0.0 0.0 0.0 0.0 15 0.011140199409569432 0.0 0.0 0.0 0.0 16 0.016710299114354146 0.0 0.0 0.0 0.0 17 0.022280398819138864 0.0 0.0 0.0 0.0 18 0.03342059822870829 0.0 0.0 0.0 0.0 19 0.03899069793349301 0.0 0.0 0.0 0.0 20 0.04456079763827773 0.0 0.0 0.0 0.0 21 0.05570099704784716 0.0 0.0 0.0 0.0 22 0.05570099704784716 0.0 0.0 0.0 0.0 23 0.06684119645741658 0.0 0.0 0.0 0.0 24 0.07241129616220131 0.0 0.0 0.0 0.0 25 0.07241129616220131 0.0 0.0 0.0 0.0 26 0.07241129616220131 0.0 0.0 0.0 0.0 27 0.07798139586698602 0.0 0.0 0.0 0.0 28 0.08912159527655546 0.0 0.0 0.0 0.0 29 0.08912159527655546 0.0 0.0 0.0 0.0 30 0.08912159527655546 0.0 0.0 0.0 0.0 31 0.08912159527655546 0.0 0.0 0.0 0.0 32 0.08912159527655546 0.0 0.0 0.0 0.0 33 0.08912159527655546 0.0 0.0 0.0 0.0 34 0.08912159527655546 0.0 0.0 0.0 0.0 35 0.08912159527655546 0.0 0.0 0.0 0.0 36 0.08912159527655546 0.0 0.0 0.0 0.0 37 0.08912159527655546 0.0 0.0 0.0 0.0 38 0.08912159527655546 0.0 0.0 0.0 0.0 39 0.08912159527655546 0.0 0.0 0.0 0.0 40 0.08912159527655546 0.0 0.0 0.0 0.0 41 0.08912159527655546 0.0 0.0 0.0 0.0 42 0.10583189439090959 0.0 0.0 0.0 0.0 43 0.11140199409569432 0.0 0.0 0.0 0.0 44 0.11697209380047903 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTTGCT 15 0.007844801 50.710224 39 TTACTTT 15 0.007844801 50.710224 41 CAACCTG 20 3.7979707E-4 50.710224 41 AGACCCT 20 3.7979707E-4 50.710224 40 GCCAACC 15 0.007844801 50.710224 39 TTTGCTT 15 0.007844801 50.710224 40 CGACCCT 15 0.007844801 50.710224 40 CCGGAGA 15 0.008113586 50.28169 36 CCAACAC 45 1.1459633E-10 50.28169 43 CGGAGAC 15 0.008113586 50.28169 37 TTTTCAT 15 0.008113586 50.28169 36 TTTTTCA 15 0.008113586 50.28169 35 CAACACA 40 2.3155735E-9 50.28169 44 TACTTTT 15 0.008113586 50.28169 42 CGATCCT 15 0.008113586 50.28169 36 GTGCAAT 50 5.456968E-12 50.0 49 GCGTGCA 55 0.0 50.0 47 GCCAGGA 25 2.0052737E-5 50.0 15 CGTGCAA 50 5.456968E-12 50.0 48 CTTTTGA 15 0.008296542 49.999996 30 >>END_MODULE