FastQCFastQC Report
Fri 17 Jun 2016
SRR1526369_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526369_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35931
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG3010.8377167348529125No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG2140.5955859842475857No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2040.5677548634883527No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1650.45921349252734406No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG1540.4285992596921878No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG1240.3451058974144889No Hit
CAACAAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC750.2087334056942473No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG640.17811917285909104No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG530.14750494002393477No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC490.13637249172024157No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG440.12245693134062509No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG440.12245693134062509No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCAGTCTCAAAGATTAAG430.11967381926470179No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG400.11132448303693189No Hit
CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG390.1085413709610086No Hit
CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA360.10019203473323872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAACC203.9467087E-450.4590438
GCCCGTC402.3592293E-950.4590438
GCCTGTC451.1823431E-1050.4590438
TTCATTT150.00806735450.45903838
TCATTTT150.00806735450.45903839
TTAATTT150.00806735450.45903838
AAACCAC203.974369E-450.3878737
TTTAATT150.00811258250.38786737
CAACACA505.456968E-1250.24613644
CCTCAAA251.9879502E-550.24613643
CCAACAC309.852956E-750.2461343
GCCTCAA550.050.2461342
CCTTGTC150.00820360450.2461343
TATTGTC150.00820360450.2461342
ACAACAC150.00820360450.2461343
TATGCCC402.4811015E-950.17556435
CACGATT451.2369128E-1050.17556447
ACGATTA850.050.17556448
CGATCCT252.0046049E-550.17556436
CGATTAA850.050.17556449