##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526369_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 35931 Sequences flagged as poor quality 0 Sequence length 56 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.67073001029752 39.0 38.0 40.0 35.0 40.0 2 36.581948735075564 39.0 38.0 39.0 32.0 40.0 3 36.396315159611476 39.0 38.0 39.0 31.0 40.0 4 36.410175057749576 39.0 36.0 39.0 30.0 40.0 5 36.06929949069049 39.0 38.0 39.0 30.0 40.0 6 34.77968884806991 38.0 35.0 39.0 18.0 40.0 7 36.209985806128415 38.0 36.0 39.0 30.0 40.0 8 36.29456458211572 38.0 36.0 39.0 30.0 40.0 9 36.43085358047369 38.0 36.0 39.0 31.0 40.0 10 35.373883276279535 38.0 35.0 39.0 27.0 40.0 11 34.08794634159918 37.0 32.0 39.0 25.0 40.0 12 31.904372269071274 35.0 28.0 39.0 16.0 40.0 13 31.461328657705046 35.0 27.0 39.0 15.0 40.0 14 30.774901895299323 35.0 26.0 39.0 13.0 40.0 15 31.50296401436086 35.0 27.0 39.0 15.0 40.0 16 31.983412652027496 36.0 29.0 39.0 16.0 40.0 17 32.20661824051655 36.0 29.0 39.0 18.0 40.0 18 32.400434165483844 36.0 29.0 39.0 19.0 40.0 19 33.20208176783279 36.0 30.0 38.0 23.0 40.0 20 33.30516823912499 36.0 30.0 39.0 23.0 40.0 21 35.15930533522585 38.0 35.0 39.0 27.0 40.0 22 34.79605354707634 38.0 34.0 39.0 26.0 40.0 23 35.02694052489494 38.0 34.0 39.0 27.0 40.0 24 34.53967326264229 38.0 33.0 39.0 25.0 40.0 25 34.92836269516573 38.0 34.0 39.0 27.0 40.0 26 35.33714619687735 38.0 35.0 39.0 29.0 40.0 27 35.35139573070607 38.0 35.0 39.0 29.0 40.0 28 35.07133116250591 38.0 35.0 39.0 28.0 40.0 29 35.01622554340263 38.0 35.0 39.0 27.0 40.0 30 34.704767470986056 38.0 34.0 39.0 27.0 40.0 31 34.365283459964935 38.0 34.0 39.0 25.0 40.0 32 33.2996298460939 38.0 33.0 39.0 18.0 40.0 33 34.475522529292256 38.0 34.0 39.0 26.0 40.0 34 34.25404247029028 38.0 34.0 39.0 25.0 40.0 35 34.26770755058306 38.0 34.0 39.0 26.0 40.0 36 32.10965461579138 35.0 31.0 38.0 22.0 39.0 37 31.9440872783947 35.0 31.0 38.0 21.0 39.0 38 31.307422559906488 35.0 30.0 38.0 17.0 39.0 39 29.336533912220645 34.0 27.0 37.0 5.0 39.0 40 25.24858757062147 31.0 12.0 36.0 4.0 39.0 41 21.424758565027414 25.0 9.0 32.0 4.0 36.0 42 25.400322841000808 30.0 21.0 33.0 4.0 36.0 43 20.90634827864518 24.0 8.0 32.0 4.0 36.0 44 22.482090673791433 27.0 10.0 33.0 4.0 36.0 45 25.131529876708136 30.0 20.0 33.0 4.0 36.0 46 24.957084411789264 30.0 15.0 35.0 4.0 38.0 47 24.291697976677522 29.0 10.0 35.0 4.0 38.0 48 24.29720853858785 29.0 11.0 35.0 4.0 38.0 49 21.78302858256102 26.0 5.0 35.0 4.0 38.0 50 24.17063260137486 29.0 4.0 35.0 4.0 38.0 51 25.758119729481507 32.0 4.0 38.0 4.0 39.0 52 25.646099468425593 32.0 4.0 38.0 4.0 39.0 53 25.195402298850574 31.0 4.0 37.0 4.0 39.0 54 24.28131696863433 31.0 4.0 37.0 4.0 39.0 55 24.476190476190474 31.0 4.0 37.0 4.0 39.0 56 24.015084467451505 31.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.7982005131667762 1 2 2.1575531352020434 1 3 2.2646618908797933 1 4 2.4556125551527117 1 5 2.7014210285637787 1 6 4.684848965238913 1 7 2.5987876064151294 1 8 2.372113247803277 1 9 2.498803202285707 1 10 3.4174523655300604 1 11 -2.3321726404911693 1 12 -6.15027079308674 1 13 -18.711440417936885 1 14 -15.902907258054997 1 15 -9.720039365968603 1 16 -19.34470861837156 1 17 -20.067689449385266 1 18 -19.896775633660244 1 19 -6.236950658026476 1 20 -11.41737110867766 1 21 -8.79700296889973 1 22 -4.1056435984617075 1 23 -5.430269465220707 1 24 -4.824776598580005 1 25 -4.360703826217577 1 26 1.0680583850335736 1 27 0.10473814051220387 1 28 0.9720593162459252 1 29 -0.13269163073879753 1 30 1.1411842463244781 1 31 -0.8128869925344091 1 32 -11.782839671092951 1 33 -1.884422084321951 1 34 -1.5492491384768705 1 35 -1.1745750813385811 1 36 1.79121278922279 1 37 2.3369874595075224 1 38 3.1490245399732224 1 39 6.077792212585756 1 40 6.353256969779498 1 41 -8.882865417657374 1 42 -13.719280561028675 1 43 -13.304032826305562 1 44 -15.658732764972832 1 45 -15.533663800126034 1 46 -18.382458304886004 1 47 -17.94707417608901 1 48 -16.714734234541638 1 49 -15.32448695579569 1 50 -17.176396324590645 1 51 -18.633464307472174 1 52 -18.68114933703195 1 53 -18.11430303097332 1 54 -17.88792551221342 1 55 -17.75266853761741 1 56 -17.218586672557272 2 1 0.6007377442754347 2 2 1.9529199305688394 2 3 2.2089531208853046 2 4 1.7076809500782488 2 5 2.544223014222908 2 6 4.196156190831857 2 7 1.8784346003478376 2 8 2.1062555533741545 2 9 1.908069611552115 2 10 3.1295317918951966 2 11 -1.4794423591894592 2 12 -7.586012657400033 2 13 -10.745637880705775 2 14 -13.676211174216055 2 15 -8.70018277468344 2 16 -4.384421800941883 2 17 -3.267910078177323 2 18 -10.570245021415346 2 19 -2.4090411158312186 2 20 -4.514999349163045 2 21 2.4716125854301083 2 22 3.0752720110253335 2 23 2.9823063759265622 2 24 2.5602206435600934 2 25 2.2973215460935066 2 26 2.017865334840522 2 27 2.56474917195181 2 28 2.1783472368195618 2 29 2.119928336166879 2 30 2.1626955535500727 2 31 -0.9187888127219495 2 32 -9.732095048919756 2 33 -0.5942952227665188 2 34 0.08726492660862561 2 35 -0.35052654300432806 2 36 4.413937554804697 2 37 4.622701745221807 2 38 5.060221451170133 2 39 8.311107160186417 2 40 10.453091498185454 2 41 4.97372586750534 2 42 1.3005760302564866 2 43 -2.866084674071697 2 44 -9.293040542137753 2 45 -2.3990802369710416 2 46 -11.352121845978116 2 47 -12.531188903060878 2 48 -9.004309524116927 2 49 -10.358132846584438 2 50 -9.110207686973435 2 51 -8.070309315745757 2 52 -8.771607141775469 2 53 -8.899189958717391 2 54 -14.09807443664806 2 55 -7.823269751075765 2 56 -7.492717946688545 3 1 1.1472614384988873 3 2 2.526769809503463 3 3 2.8097832834588132 3 4 2.5879991790647594 3 5 3.097664729892223 3 6 4.590483413246247 3 7 2.388984583052789 3 8 2.3684485661724963 3 9 2.2688444299540507 3 10 2.9112682152781133 3 11 2.0283404149371407 3 12 2.6834442779164682 3 13 -5.108142204469178 3 14 -4.617062090853899 3 15 -3.5033650645485395 3 16 2.7982139652289924 3 17 2.3477938305047914 3 18 2.154071823966877 3 19 1.365431385033535 3 20 0.7644887172499608 3 21 3.191086815800226 3 22 3.3506092645708208 3 23 2.599047987825557 3 24 2.6145271119101494 3 25 2.807871528798458 3 26 2.175250322733987 3 27 1.8051504171956694 3 28 2.274853635989402 3 29 1.8677664544650483 3 30 1.5213949655182475 3 31 2.907222947914512 3 32 1.181429683006911 3 33 2.7381157077073652 3 34 2.3747087176843777 3 35 2.8258830038652647 3 36 5.301519706309371 3 37 4.286140001880401 3 38 5.423417577078123 3 39 7.712698305118963 3 40 9.762327056815682 3 41 8.910080070203367 3 42 6.158410689543931 3 43 1.0294532020957199 3 44 1.245527427422953 3 45 6.1571286872766215 3 46 -2.163951662650547 3 47 -6.387294056986855 3 48 -2.0963192093469516 3 49 -11.022608806459914 3 50 -3.9739226644898658 3 51 -5.705383684476299 3 52 -5.553343565158382 3 53 -4.990161940730534 3 54 -6.114677688118141 3 55 -4.8319172778831 3 56 -2.54840801932777 4 1 1.2390206436420783 4 2 2.7213928369727824 4 3 2.8331614248965664 4 4 2.4532826390296876 4 5 3.464701550464987 4 6 4.764066113421578 4 7 2.23205880865185 4 8 2.5911253633671194 4 9 2.646985867709752 4 10 2.517638758819899 4 11 4.85095876677817 4 12 6.145162571312138 4 13 6.384552126331524 4 14 5.427940831413782 4 15 7.6024210254573035 4 16 6.751749909121454 4 17 6.554398155460959 4 18 6.034258849098375 4 19 5.353450087546687 4 20 5.140876794397826 4 21 3.3372001997861958 4 22 4.127348013840255 4 23 2.903840506820302 4 24 3.8737322551012667 4 25 3.2716354095699316 4 26 2.9981609013429846 4 27 2.431392380959551 4 28 2.664976371231944 4 29 2.5770008203329624 4 30 2.3238496703692135 4 31 3.0477840233090205 4 32 3.2921836280682797 4 33 3.346239611856987 4 34 2.159045362921077 4 35 2.4833932318021468 4 36 4.28515500730294 4 37 3.9921598733006256 4 38 5.478940662992795 4 39 7.539115604943632 4 40 9.21206405272451 4 41 4.991319111699568 4 42 7.103179830397234 4 43 7.334830174626404 4 44 9.446579443787652 4 45 6.682552416090182 4 46 5.744289132206259 4 47 5.86606655084482 4 48 5.904557470956966 4 49 1.4490451970468072 4 50 6.473184290507213 4 51 4.58275190874404 4 52 4.564423822334312 4 53 4.436489140508506 4 54 -0.8581025858387719 4 55 4.211916737312691 4 56 3.1131933833607164 5 1 2.0559967709630342 5 2 3.3924803701292774 5 3 3.376928003145899 5 4 3.2284815780217357 5 5 3.847990675133424 5 6 5.580379110769059 5 7 2.7983718059993308 5 8 3.068578944268971 5 9 2.6827948862773923 5 10 3.414853612931303 5 11 5.64339908508056 5 12 7.615321722505772 5 13 8.738663532167063 5 14 8.138153033005292 5 15 8.457195561266325 5 16 7.78159075792782 5 17 7.614742983047165 5 18 7.026301289416676 5 19 5.887269716193902 5 20 5.683980242673691 5 21 4.331895159998396 5 22 4.08822334540524 5 23 3.72877419382295 5 24 4.157551883748482 5 25 3.1787972132834597 5 26 2.715667532642719 5 27 2.598501134275196 5 28 2.1092734534600623 5 29 2.847557849510679 5 30 3.2290220841623167 5 31 3.543142113494696 5 32 5.161547023293743 5 33 3.6592369593370933 5 34 4.3254909862632545 5 35 3.6710590142955155 5 36 5.404518402528403 5 37 5.298525921045982 5 38 5.818463209411892 5 39 8.93434106913461 5 40 6.735273601796127 5 41 4.33283104803909 5 42 9.515646673898559 5 43 12.990665718392982 5 44 12.103741247501176 5 45 10.086398569936335 5 46 10.292697620269923 5 47 9.917790688775856 5 48 10.718217948410544 5 49 10.890689759381026 5 50 10.536181637987319 5 51 10.0442903702825 5 52 9.423840267957655 5 53 10.533306116635824 5 54 9.927043302755393 5 55 10.318017533068662 5 56 9.367172163201566 6 1 1.4374724381529873 6 2 3.095777073425978 6 3 3.004871488232247 6 4 2.421966664363964 6 5 3.625855667284128 6 6 5.441054150015525 6 7 2.71516929422539 6 8 2.7389086145986425 6 9 3.010110427878651 6 10 3.741776689854383 6 11 4.887904579586049 6 12 7.862966934436702 6 13 7.458443751947289 6 14 8.527870458437004 6 15 8.29981722531656 6 16 7.125782280690768 6 17 7.466783799373694 6 18 6.674652937768325 6 19 5.713407863760619 6 20 5.382959834510423 6 21 3.675694218083173 6 22 4.095680174290635 6 23 3.8802655596000335 6 24 4.070424725192751 6 25 3.7667093011955473 6 26 3.2627632940241966 6 27 2.4627083556252742 6 28 3.5456941755950737 6 29 2.1403364994321876 6 30 3.0402465739582354 6 31 3.142435677073969 6 32 4.288313114345549 6 33 3.8955006956008305 6 34 3.9444077837514797 6 35 4.24131019168955 6 36 3.4343457180700057 6 37 3.6839262350177258 6 38 3.815323491986465 6 39 6.086617364268051 6 40 0.8408466002262678 6 41 0.4635217858726861 6 42 8.055678071072812 6 43 12.664527570826262 6 44 10.360020682352044 6 45 8.213164660988141 6 46 11.178490398919845 6 47 11.12187232142892 6 48 10.87324149629124 6 49 12.009214092191073 6 50 10.012241292618402 6 51 9.746017214866487 6 52 10.207984694959226 6 53 10.815095755568326 6 54 10.53457862457643 6 55 10.258362901985457 6 56 9.548098379842067 7 1 1.6010896091593168 7 2 3.2213928369727824 7 3 3.126264873172424 7 4 2.66017919075383 7 5 3.3440118952925744 7 6 4.919238527214681 7 7 2.1458519121001274 7 8 2.280780535780913 7 9 2.6125031090890616 7 10 3.1555697933026607 7 11 3.8337173874678214 7 12 6.610679812691448 7 13 6.884552126331524 7 14 8.41069945210344 7 15 8.033455508215923 7 16 6.734508529811105 7 17 6.433708500288546 7 18 6.223914021512169 7 19 4.491381122029441 7 20 5.244325070259897 7 21 2.819958820475854 7 22 3.4721756000471444 7 23 2.627978437854779 7 24 3.304766737859886 7 25 2.426807823363035 7 26 1.9119540047912622 7 27 2.483116518890583 7 28 2.233941888473325 7 29 1.3528628892984784 7 30 1.9962634634726584 7 31 2.116749540550394 7 32 2.82666638668897 7 33 2.4324465084087095 7 34 1.9521488111969418 7 35 1.2592553007676628 7 36 -0.6803622340763695 7 37 -0.4043918508373068 7 38 0.6858372147169334 7 39 2.9184259497712155 7 40 -3.4431083610685924 7 41 -8.784542957265948 7 42 5.206628106259302 7 43 9.817588795316059 7 44 8.032786340339374 7 45 4.941173105745353 7 46 7.296013270137294 7 47 7.969514826706892 7 48 7.370074712336276 7 49 10.052493472908875 7 50 7.214563600852038 7 51 8.375855357019901 7 52 8.340285891299832 7 53 7.453730519818851 7 54 8.93500086243709 7 55 5.746399495933382 7 56 5.475262348877958 8 1 1.9252275401938022 8 2 2.8786342162831247 8 3 3.2938510800689826 8 4 2.801558501098654 8 5 3.7295291366718857 8 6 5.142686803076749 8 7 2.8906794983070228 8 8 2.5931943288843584 8 9 2.696641040123545 8 10 4.001776689854381 8 11 5.536476008157479 8 12 7.463783260967315 8 13 8.515586609090146 8 14 7.69276841762068 8 15 8.46104171511248 8 16 6.073129219466278 8 17 6.524742983047162 8 18 6.883224366339757 8 19 5.9203466392708215 8 20 5.993980242673693 8 21 3.0365105446137832 8 22 3.0997618069436967 8 23 2.4010818861306404 8 24 4.2206288068254025 8 25 3.869566444052694 8 26 3.346436763411951 8 27 3.1431165188905794 8 28 2.6877349919216016 8 29 2.7121732341260625 8 30 3.299022084162317 8 31 3.3946805750331563 8 32 4.917700869447586 8 33 4.366929267029398 8 34 3.7431832939555605 8 35 3.5102897835262823 8 36 1.3706722486822471 8 37 1.6369874595075196 8 38 2.063078594027278 8 39 3.7404949152884583 8 40 -6.46241870589618 8 41 -9.701784336576294 8 42 4.461800520052407 8 43 8.497588795316059 8 44 7.624510478270409 8 45 4.622552416090183 8 46 10.17808223565454 8 47 10.377790688775857 8 48 9.452833333025932 8 49 12.681458990150258 8 50 9.173873945679624 8 51 10.782751908744043 8 52 10.456147960265351 8 53 11.044075347405059 8 54 10.943966379678468 8 55 10.325709840760972 8 56 9.898710624740026 9 1 1.2780577288730441 9 2 2.1820304426982204 9 3 2.850077495163319 9 4 2.2128792558156363 9 5 2.8472649857284864 9 6 4.428347180435246 9 7 1.6604908190617422 9 8 2.038099989261717 9 9 1.9215467005009046 9 10 1.7040408407977807 9 11 3.8070420458933256 9 12 6.035858732665428 9 13 5.126907363807124 9 14 7.0180514364886015 9 15 6.6629285075653115 9 16 5.587468842107786 9 17 5.08172411512264 9 18 4.499450781434096 9 19 3.060346639270822 9 20 3.8207726955038765 9 21 1.6599067710288793 9 22 1.658252372981437 9 23 2.053157357828759 9 24 1.9579872973914405 9 25 1.985038142165898 9 26 0.6728518577515743 9 27 1.2755693490792623 9 28 1.8364142372046217 9 29 1.2982109699751234 9 30 1.7148711407660926 9 31 2.358454159938816 9 32 2.7765687939758905 9 33 1.806551908538836 9 34 2.052994614710279 9 35 1.2940633684319423 9 36 0.06199300339923042 9 37 0.7898176481867694 9 38 1.82572010346124 9 39 2.271060953024307 9 40 -0.22807908325466997 9 41 -4.348954147897047 9 42 5.9655741049580655 9 43 9.713815210410399 9 44 9.056208591477958 9 45 5.022175057599615 9 46 8.327893556409254 9 47 7.144205783115478 9 48 7.64528616321461 9 49 9.017685405244595 9 50 7.641043757000379 9 51 6.526148135159133 9 52 6.5784121112087455 9 53 6.698037611556 9 54 5.79641920986715 9 55 5.361558897364741 9 56 5.908899303985308 10 1 2.138953030389878 10 2 3.57196754961646 10 3 3.669537354578786 10 4 3.6956761481574816 10 5 4.162470313142471 10 6 5.483471116802242 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2.2238572596313233 115 28 1.6847720289586405 115 29 2.472913974866806 115 30 0.9049480100882406 115 31 0.5324583528109343 115 32 -6.723780612033892 115 33 0.9484107485108808 115 34 1.1424425532148206 115 35 0.3199194131559082 115 36 2.6580796560896545 115 37 3.4110615335815986 115 38 3.499374890323576 115 39 5.669383804177343 115 40 9.084988701511225 115 41 10.64266010786815 115 42 6.324763483015367 115 43 6.5768480545753185 115 44 4.806732700492631 115 45 6.97884871238648 115 46 -0.175251097678796 115 47 -2.6748019038167357 115 48 -1.9249444447518478 115 49 -3.971133602442338 115 50 1.501281353087034 115 51 2.916085242077372 115 52 2.505036849154237 115 53 2.0418531251828362 115 54 1.5595219352340273 115 55 3.2923765074276368 115 56 5.3379698839992855 116 1 0.9814775401938007 116 2 1.988634216283124 116 3 2.2663510800689792 116 4 2.3703085010986555 116 5 2.653279136671884 116 6 3.647686803076752 116 7 1.8656794983070242 116 8 1.7969443288843578 116 9 0.8366410401235456 116 10 1.8042766898543832 116 11 0.19147600815747978 116 12 -2.1587167390326876 116 13 -5.434413390909857 116 14 -9.445981582379321 116 15 -3.326458284887522 116 16 -10.97562078053372 116 17 -8.104007016952835 116 18 -3.865525633660244 116 19 -4.252153360729178 116 20 -4.066019757326309 116 21 0.9040105446137829 116 22 1.4872618069436996 116 23 0.45233188613064357 116 24 0.549378806825402 116 25 1.3470664440526932 116 26 1.5089367634119526 116 27 2.233116518890583 116 28 2.0852349919216024 116 29 2.2009232341260656 116 30 2.1040220841623167 116 31 -0.12031942496684422 116 32 -9.83604913055241 116 33 -0.7205707329706001 116 34 0.32068329395556106 116 35 0.002789783526282008 116 36 2.8444222486822497 116 37 4.086987459507522 116 38 2.8280785940272786 116 39 5.7804949152884575 116 40 8.20883129410382 116 41 5.8232156634237064 116 42 2.4243005200524053 116 43 -7.526161204683941 116 44 1.6470104782704098 116 45 3.870052416090182 116 46 -4.560667764345464 116 47 -9.082209311224144 116 48 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120 15 -5.638958284887522 120 16 -5.173537447200388 120 17 -7.153775535471354 120 18 -2.6375163744009846 120 19 -2.5692829903588077 120 20 -3.399353090659641 120 21 -0.20941538131214088 120 22 0.8553173624992567 120 23 0.25441521946397927 120 24 -4.086037859841266 120 25 0.31697385146009793 120 26 1.0019923189675097 120 27 1.075709111483178 120 28 0.9440312882179 120 29 1.2136547156075466 120 30 1.3123554174956524 120 31 -0.20828238792980613 120 32 -11.316373204626483 120 33 -1.0886262885261573 120 34 -7.33903892826666 120 35 -1.828228734992237 120 36 -0.19377219576219318 120 37 -0.5148643923443288 120 38 -0.16729177634309167 120 39 1.8916060263995682 120 40 -2.211307594785069 120 41 -2.7647472995392555 120 42 -7.119680961429076 120 43 -6.386114908387645 120 44 -4.4525265587666265 120 45 -12.317447583909818 120 46 -13.360436282863983 120 47 -14.045172274187108 120 48 -12.51753703734444 120 49 -14.600763232071966 120 50 -11.906126054320374 120 51 -12.269099943107811 120 52 -12.272740928623541 120 53 -12.772961689631979 120 54 -12.403441027728936 120 55 -13.337253122201995 120 56 -10.47684493081553 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 518.0 5 37.0 6 29.0 7 40.0 8 49.0 9 42.0 10 29.0 11 38.0 12 56.0 13 71.0 14 99.0 15 134.0 16 225.0 17 325.0 18 377.0 19 389.0 20 481.0 21 650.0 22 755.0 23 878.0 24 984.0 25 1130.0 26 1119.0 27 1116.0 28 1217.0 29 1347.0 30 1435.0 31 1794.0 32 2059.0 33 2520.0 34 3005.0 35 3523.0 36 3829.0 37 3448.0 38 1687.0 39 496.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 100.0 0.0 0.0 3 0.0 100.0 0.0 0.0 4 0.0 0.0 0.0 100.0 5 0.0 100.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.46842559349865 0.4759121649828839 0.02226489660738638 0.03339734491107957 8 99.58531630068744 0.3534552336422587 0.03339734491107957 0.027831120759232972 9 99.20104671232114 0.5317075886643282 0.11970380268359222 0.14754189633093925 10 50.315883220617295 25.94417077175698 14.547326820851076 9.192619186774651 11 29.7355969941553 35.31867520178124 17.25020873921514 17.695519064848316 12 28.713367287300663 13.523141576911302 20.369597283682612 37.39389385210543 13 22.74637499652111 13.178035679496814 23.24455205811138 40.8310372658707 14 19.930410577592205 16.8044537230341 31.74391092553932 31.521224773834376 15 29.19791463380635 19.097270623658314 22.417686581727956 29.28712816080738 16 30.722975418279002 19.75167729183486 23.629631691768047 25.89571559811809 17 23.642234779945994 21.816106672605294 29.07607939203296 25.465579155415753 18 24.804453723034097 25.10229645093946 28.501043841336116 21.592205984690327 19 32.479679323015255 25.242177931188063 18.466763166685222 23.81137957911146 20 24.219054513057518 32.37374018597918 19.160309594075393 24.246895706887912 21 23.856045424181698 23.51369405477622 28.98296593186373 23.647294589178355 22 23.175619259671585 22.424158085165597 23.055942109657668 31.34428054550515 23 24.279869750354845 24.201942612228994 21.449444769140854 30.068742868275304 24 22.042247641312514 24.18246082769753 29.715287634633047 24.060003896356907 25 30.297801280267183 26.38185360423045 21.07987753966045 22.240467575841915 26 22.912659251205707 25.736667502996852 22.171113155473783 29.17956009032366 27 21.421811501419587 21.920057896787842 23.403663085230754 33.25446751656182 28 20.632840814050738 31.137440758293838 25.639810426540283 22.589908001115138 29 28.73726407215634 22.111797784087745 26.77189466065364 22.379043483102276 30 19.442202243438082 23.33620953600356 37.99649288835695 19.22509533220141 31 18.930224775503373 31.00842210943165 30.868983211556696 19.192369903508283 32 30.280004453351143 19.956579826319302 29.041416165664664 20.721999554664887 33 21.005380990882987 21.908718320461706 29.9690523322274 27.116848356427916 34 20.854572296036324 29.19138742653408 32.09938441826133 17.85465585916827 35 20.66939548352964 22.746637707793834 40.77353604544315 15.810430763233368 36 20.780379041248604 30.515607580824973 30.515607580824973 18.18840579710145 37 19.70377395330674 22.760871384340746 38.54843658475357 18.98691807759895 38 28.94685505671209 25.34347741270239 30.501908981969176 15.207758548616338 39 19.50258488193377 19.59759675841833 28.031298029900796 32.868520329747106 40 11.794871794871794 15.33697632058288 27.745551352108727 45.12260053243659 41 16.032830199286057 16.800179891502935 29.72144925092054 37.44554065829047 42 24.749686236229255 23.385859712731836 32.21865848556686 19.645795565472042 43 11.849824012514667 13.542656014302477 29.817308229510033 44.79021174367283 44 12.34747565207657 15.26362923989701 23.961714989365273 48.42718011866115 45 18.224624557688557 22.749435791702656 40.202279122899895 18.823660527708896 46 17.316475437228473 23.50172663473321 33.69165645538599 25.490141472652333 47 15.8675799086758 26.60095779039982 32.077625570776256 25.453836730148122 48 19.03488987274803 29.76359535544232 32.626068554562416 18.575446217247237 49 12.46544357878864 19.220351288710173 23.517913490268352 44.79629164223283 50 23.02264614011602 22.97244533690317 35.35531012940651 18.649598393574294 51 20.14183605092696 29.36955550591914 34.177462586553496 16.3111458566004 52 18.077675328303997 24.26376082704666 37.37636211232188 20.282201732327465 53 18.503333054416647 25.119236883942765 38.62438289682872 17.75304716481187 54 17.838033664028536 26.727789544086498 28.67573291717757 26.75844387470739 55 17.989181955054924 30.4104165504935 33.86494172754141 17.735459766910168 56 24.8535891572313 23.227731608009368 31.878520832171343 20.040158402587984 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 2.5 5 3.0 6 3.5 7 4.0 8 3.5 9 3.0 10 11.0 11 19.0 12 52.5 13 144.0 14 202.0 15 252.0 16 302.0 17 334.0 18 366.0 19 383.5 20 401.0 21 439.0 22 520.0 23 563.0 24 638.0 25 713.0 26 807.5 27 902.0 28 1017.0 29 1157.5 30 1183.0 31 1089.0 32 995.0 33 914.5 34 834.0 35 734.5 36 635.0 37 567.5 38 515.5 39 531.0 40 543.0 41 555.0 42 575.0 43 595.0 44 905.5 45 1216.0 46 1614.5 47 1877.0 48 1741.0 49 1586.0 50 1431.0 51 1451.0 52 1471.0 53 1528.5 54 1599.0 55 1612.0 56 1736.5 57 1861.0 58 1906.0 59 1951.0 60 1984.5 61 2018.0 62 1930.5 63 1790.5 64 1738.0 65 1514.5 66 1291.0 67 1105.5 68 920.0 69 836.0 70 752.0 71 663.0 72 479.5 73 385.0 74 310.5 75 236.0 76 186.5 77 137.0 78 112.0 79 65.5 80 44.0 81 27.0 82 10.0 83 9.0 84 8.0 85 4.0 86 0.0 87 1.0 88 1.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.02504800868330968 10 0.0 11 0.0027831120759232975 12 0.0 13 0.0 14 0.016698672455539784 15 0.17255294870724444 16 0.027831120759232972 17 0.02226489660738638 18 0.016698672455539784 19 0.01948178453146308 20 0.03618045698700287 21 0.008349336227769892 22 0.0027831120759232975 23 0.0 24 0.0 25 0.0027831120759232975 26 0.16698672455539784 27 0.013915560379616486 28 0.16976983663132114 29 0.02504800868330968 30 0.01113244830369319 31 0.2031671815424007 32 0.008349336227769892 33 0.17811917285909104 34 0.08349336227769892 35 0.05009601736661936 36 0.14193871587208817 37 0.22264896607386378 38 0.13358937964431827 39 0.4063343630848014 40 0.6846455706771312 41 0.9852216748768473 42 0.2115165177701706 43 0.3701539060977985 44 0.5538393031087362 45 0.11132448303693189 46 0.06401157774623584 47 0.04174668113884946 48 0.05009601736661936 49 0.3339734491107957 50 0.2087334056942473 51 0.3200578887311792 52 0.392418802705185 53 0.2170827419220172 54 0.13080626756839497 55 0.18090228493501434 56 0.2031671815424007 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 35931.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.10929281122151 #Duplication Level Percentage of deduplicated Percentage of total 1 92.92411760766191 77.22857699479559 2 4.487308284776639 7.458740363474437 3 1.2155917219208359 3.030809050680471 4 0.5056593664188601 1.6809996938576717 5 0.22436541423883197 0.9323425454343046 6 0.11720581340834506 0.5844535359438925 7 0.11050833835643961 0.6428988895382818 8 0.10381086330453419 0.6902117948289778 9 0.053579800415243455 0.4007681389329549 >10 0.22771415176478468 3.5818652417132837 >50 0.010046212577858147 0.5343575185772731 >100 0.020092425155716295 3.233976232222872 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 301 0.8377167348529125 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 214 0.5955859842475857 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 204 0.5677548634883527 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 165 0.45921349252734406 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 154 0.4285992596921878 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG 124 0.3451058974144889 No Hit CAACAAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 75 0.2087334056942473 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG 64 0.17811917285909104 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 53 0.14750494002393477 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 49 0.13637249172024157 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG 44 0.12245693134062509 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 44 0.12245693134062509 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCAGTCTCAAAGATTAAG 43 0.11967381926470179 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG 40 0.11132448303693189 No Hit CAACAAGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG 39 0.1085413709610086 No Hit CAACAAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 36 0.10019203473323872 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.008349336227769892 0.0 0.0 0.0 0.0 7 0.008349336227769892 0.0 0.0 0.0 0.0 8 0.008349336227769892 0.0 0.0 0.0 0.0 9 0.008349336227769892 0.0 0.0 0.0 0.0 10 0.013915560379616488 0.0 0.0 0.0 0.0 11 0.016698672455539784 0.0 0.0 0.0 0.0 12 0.016698672455539784 0.0 0.0 0.0 0.0 13 0.016698672455539784 0.0 0.0 0.0 0.0 14 0.016698672455539784 0.0 0.0 0.0 0.0 15 0.01948178453146308 0.0 0.0 0.0 0.0 16 0.025048008683309676 0.0 0.0 0.0 0.0 17 0.025048008683309676 0.0 0.0 0.0 0.0 18 0.027831120759232976 0.0 0.0 0.0 0.0 19 0.030614232835156272 0.0 0.0 0.0 0.0 20 0.03339734491107957 0.0 0.0 0.0 0.0 21 0.03339734491107957 0.0 0.0 0.0 0.0 22 0.03339734491107957 0.0 0.0 0.0 0.0 23 0.03339734491107957 0.0 0.0 0.0 0.0 24 0.03618045698700287 0.0 0.0 0.0 0.0 25 0.04174668113884946 0.0 0.0 0.0 0.0 26 0.04174668113884946 0.0 0.0 0.0 0.0 27 0.04174668113884946 0.0 0.0 0.0 0.0 28 0.04731290529069605 0.0 0.0 0.0 0.0 29 0.05009601736661935 0.0 0.0 0.0 0.0 30 0.05009601736661935 0.0 0.0 0.0 0.0 31 0.05009601736661935 0.0 0.0 0.0 0.0 32 0.05009601736661935 0.0 0.0 0.0 0.0 33 0.05009601736661935 0.0 0.0 0.0 0.0 34 0.05009601736661935 0.0 0.0 0.0 0.0 35 0.05009601736661935 0.0 0.0 0.0 0.0 36 0.05009601736661935 0.0 0.0 0.0 0.0 37 0.05009601736661935 0.0 0.0 0.0 0.0 38 0.05009601736661935 0.0 0.0 0.0 0.0 39 0.05009601736661935 0.0 0.0 0.0 0.0 40 0.05287912944254265 0.0 0.0 0.0 0.0 41 0.05566224151846595 0.0 0.0 0.0 0.0 42 0.058445353594389245 0.0 0.0 0.0 0.0 43 0.058445353594389245 0.0 0.0 0.0 0.0 44 0.06401157774623584 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACAACC 20 3.9467087E-4 50.45904 38 GCCCGTC 40 2.3592293E-9 50.45904 38 GCCTGTC 45 1.1823431E-10 50.45904 38 TTCATTT 15 0.008067354 50.459038 38 TCATTTT 15 0.008067354 50.459038 39 TTAATTT 15 0.008067354 50.459038 38 AAACCAC 20 3.974369E-4 50.38787 37 TTTAATT 15 0.008112582 50.387867 37 CAACACA 50 5.456968E-12 50.246136 44 CCTCAAA 25 1.9879502E-5 50.246136 43 CCAACAC 30 9.852956E-7 50.24613 43 GCCTCAA 55 0.0 50.24613 42 CCTTGTC 15 0.008203604 50.24613 43 TATTGTC 15 0.008203604 50.24613 42 ACAACAC 15 0.008203604 50.24613 43 TATGCCC 40 2.4811015E-9 50.175564 35 CACGATT 45 1.2369128E-10 50.175564 47 ACGATTA 85 0.0 50.175564 48 CGATCCT 25 2.0046049E-5 50.175564 36 CGATTAA 85 0.0 50.175564 49 >>END_MODULE