FastQCFastQC Report
Fri 17 Jun 2016
SRR1526368_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526368_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12947
Sequences flagged as poor quality0
Sequence length56
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTACCCACAGTTCTGAGTCG2612.0159110218583454No Hit
CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG2411.8614350814860587No Hit
CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG2241.7301305321696145No Hit
CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG2211.7069591411137717No Hit
CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG2111.6297211709276278No Hit
CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGTCG1771.36711207229474No Hit
CTCGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACCCACAGTTCTGAGTCG1351.0427125975129374No Hit
CTCGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG1120.8650652660848073No Hit
CTCGCAGGGATGAGATGGACTCAAATGAATTGACGGGTACCCACAGTTCTGAGTCG1110.857341469066193No Hit
CTCGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG1080.8341700780103499No Hit
CTCGCAGGGATGCGCTGGACTTAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG1000.7723797018614351No Hit
CTCGCAGGGATGCGCTGGACTTAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG990.7646559048428208No Hit
CTCGCAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG940.7260369197497489No Hit
CTCGCAGGGATGAGATGGACTCAAATGAATTGACGGGTACGCACAGTTCTGAGTCG870.6719703406194485No Hit
CTCGCAGGGATGCGCTGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG450.3475708658376458No Hit
CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACTCACAGTTCTGAGTCG440.33984706881903143No Hit
CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTACTCACAGTTCTGAGTCG420.3243994747818027No Hit
CTCGCAGGGATGCGCTGGACTCAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG390.30122808372595966No Hit
CTCGCAGGGATGCGCTGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG370.285780489688731No Hit
CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTACACACAGTTCTGAGTCG350.27033289565150226No Hit
CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCAAGTTCTGAGTCG330.2548853016142736No Hit
CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACACACAGTTCTGAGTCG320.24716150459565922No Hit
CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTAACCACAGTTCTGAGTCG310.23943770757704486No Hit
CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTAACCACAGTTCTGAGTCG300.23171391055843055No Hit
CTCGCAGGGATGCGCTGGACTCAAATGAATTGACGGGTCCGCCCAGTTCTGAGTCG270.20854251950258748No Hit
CTCGCAGGGATGAGCTGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG250.19309492546535878No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC240.18537112844674442No Hit
CTCGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACTCACAGTTCTGAGTCG210.16219973739090135No Hit
CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGCCG200.154475940372287No Hit
CTCGCAGGGATGCGATGGACTTAAAGGAATTGACGGGTCCGCCCAGTTCTGAGTCG200.154475940372287No Hit
CTCGCAGGGATGAGCTGGACTTAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG190.14675214335367268No Hit
CTCGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTAACCACAGTTCTGAGTCG190.14675214335367268No Hit
CTCGCAGGGATGCGCTGGACTCAAAGGAATTGACGGGTACGCCCAGTTCTGAGTCG180.1390283463350583No Hit
CTCGCAGGGATGCGCTGGACTCAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG170.13130454931644397No Hit
CTCGCAGGGATGAGATGGACTCAAATGAATTGACGGGTACTCACAGTTCTGAGTCG170.13130454931644397No Hit
CTCGCAGGGATGAGATGGACTTAAAGGAATTGACGGGTACGCCAAGTTCTGAGTCG150.11585695527921527No Hit
CTCGCAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC150.11585695527921527No Hit
CTCGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACACACAGTTCTGAGTCG150.11585695527921527No Hit
CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCACAGTTCTGAGCCG140.10813315826060091No Hit
CTCGCAGGGATGAGATGGACTTAAATGAATTGACGGGTACGCCTAGTTCTGAGTCG140.10813315826060091No Hit
CTCGCAGGGATGAGCTGGACTTAAAGGAATTGACGGGTACGCACAGTTCTGAGTCG140.10813315826060091No Hit
CTCGCAGGGATGAGATGGACTCAAAGGAATTGACGGGTACGCCAAGTTCTGAGTCG140.10813315826060091No Hit
CTCGCAGGGGAGACCGCACTCGGACCTGCTCCGCTCCTGCCCCGCGGCCAAGGAGA130.10040936124198656No Hit
CTCGCAGGGATGCGCTGGACTTAAAGGAATTGACGGGTACGCCCAGCTCTGAGTCG130.10040936124198656No Hit
CTCGCAGGGATGCGATGGACTTAAATGAATTGACGGGTACGCCCAGTTCTGAGTCG130.10040936124198656No Hit
CTCGCAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC130.10040936124198656No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCAGC150.00778111650.73122841
ACACAGT203.7492448E-450.73122841
GCCAAGT203.7492448E-450.73122841
ACGCCAA251.8093573E-550.73122439
GTACCCA1100.050.73122437
GTCCGCC700.050.73122437
CCGCCCA700.050.73122439
CGCCAAG251.8093573E-550.73122440
CCCACAG1150.050.73122440
TACGCCA251.8093573E-550.73122438
TCCGCCC750.050.73122438
GGGTATC150.00790413850.53149835
GGGTACT150.00790413850.53149835
GGGTACC1250.050.53149835
GGTACTC150.00790413850.53149836
GGTACCC1200.050.53149836
CCCAGTT2300.050.1367242
CCCAGCT150.00815451850.1367242
CCAAGTT203.975638E-450.1367242
CACAGCT150.00815451850.1367242
Produced by FastQC (version 0.11.5)