FastQCFastQC Report
Fri 17 Jun 2016
SRR1526367_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526367_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28944
Sequences flagged as poor quality0
Sequence length56
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC470.16238253178551687No Hit
ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA340.11746821448313986No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTTT150.00809254550.39404738
CAACCTG150.00809254550.39404741
CCTTCTT150.00809254550.39404739
GGCTGTT150.00814885650.30594642
ACAACAC204.0279632E-450.2181543
CCAACAC309.822688E-750.21814743
GCTGTTC150.008205458550.21814743
TTTTCGT150.00826235350.1306636
GCGTGCA150.00826235350.1306647
TACAAAC150.00826235350.1306637
CCTCAGT150.00826235350.1306648
ATCCCAA150.0083195450.0434826
TCCCAAA150.0083195450.0434827
AAAGGTG150.0083195450.0434833
AAGGTGC150.0083195450.0434834
GTGAACG150.0083195450.0434826
GAAAGGT150.0083195450.0434832
AGGACAA301.0307995E-649.87001818
CCCGGAG150.00843479849.87001813
CCTTGGA150.00843479849.87001820
Produced by FastQC (version 0.11.5)