##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526367_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 28944 Sequences flagged as poor quality 0 Sequence length 56 %GC 49 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.068615257048094 38.0 38.0 39.0 33.0 40.0 2 36.437085406301826 39.0 38.0 39.0 31.0 40.0 3 36.17378385848535 39.0 37.0 39.0 30.0 40.0 4 35.82587064676617 39.0 36.0 39.0 29.0 40.0 5 36.41200248756219 39.0 38.0 39.0 31.0 40.0 6 35.95339275843007 38.0 36.0 39.0 30.0 40.0 7 36.36874654505252 38.0 37.0 39.0 32.0 40.0 8 36.20259812050857 38.0 36.0 39.0 31.0 40.0 9 36.22315505804312 38.0 36.0 39.0 31.0 40.0 10 35.90547263681592 38.0 36.0 39.0 30.0 40.0 11 34.336200939745716 38.0 32.0 39.0 26.0 40.0 12 32.085855444997236 36.0 29.0 39.0 15.0 40.0 13 31.924405749032616 36.0 29.0 39.0 13.0 40.0 14 31.53651879491432 36.0 28.0 39.0 12.0 40.0 15 32.07780541735766 36.0 29.0 39.0 15.0 40.0 16 31.804000829187398 36.0 29.0 39.0 14.0 40.0 17 32.03233830845771 36.0 29.0 38.0 16.0 40.0 18 32.13139165284688 36.0 29.0 38.0 18.0 40.0 19 33.003489496959645 36.0 31.0 38.0 23.0 40.0 20 32.98386539524599 36.0 30.0 38.0 22.0 40.0 21 34.421814538419014 38.0 34.0 39.0 25.0 40.0 22 34.20702045328911 38.0 33.0 39.0 24.0 40.0 23 34.267758430071865 38.0 33.0 39.0 25.0 40.0 24 33.932766721945825 38.0 33.0 39.0 23.0 40.0 25 34.13951077943615 38.0 33.0 39.0 24.0 40.0 26 34.526568546158096 38.0 35.0 39.0 26.0 40.0 27 34.40847844112769 38.0 35.0 39.0 25.0 40.0 28 34.35261194029851 38.0 34.0 39.0 25.0 40.0 29 34.219561912658925 38.0 34.0 39.0 24.0 40.0 30 34.119575732448865 38.0 34.0 39.0 24.0 40.0 31 33.68981481481482 38.0 33.0 39.0 23.0 40.0 32 32.65505804311775 38.0 32.0 39.0 13.0 40.0 33 33.825110558319516 38.0 33.0 39.0 23.0 40.0 34 33.54716003316749 38.0 33.0 39.0 21.0 40.0 35 33.5480237700387 38.0 33.0 39.0 22.0 40.0 36 31.37054311774461 35.0 30.0 38.0 10.0 39.0 37 31.079601990049753 35.0 30.0 38.0 4.0 39.0 38 30.464794085129906 35.0 29.0 38.0 4.0 39.0 39 28.516480099502488 33.0 25.0 38.0 4.0 39.0 40 24.326527086788282 30.0 10.0 37.0 4.0 39.0 41 20.915595632946378 24.0 7.0 32.0 4.0 36.0 42 24.708644278606965 30.0 18.0 33.0 4.0 37.0 43 20.765374516307354 24.0 6.0 32.0 4.0 36.0 44 21.78102542841349 26.0 7.0 33.0 4.0 36.0 45 24.39807904919845 30.0 16.0 33.0 4.0 37.0 46 24.460544499723603 30.0 4.0 35.0 4.0 38.0 47 23.68801824212272 29.0 4.0 35.0 4.0 38.0 48 23.695999170812602 29.0 4.0 35.0 4.0 38.0 49 21.03527501381979 25.0 4.0 35.0 4.0 38.0 50 23.45995715865119 29.0 4.0 35.0 4.0 38.0 51 24.966383360972912 32.0 4.0 38.0 4.0 39.0 52 24.771455223880597 31.0 4.0 38.0 4.0 39.0 53 24.318960751796574 31.0 4.0 37.0 4.0 39.0 54 23.473189607517966 30.0 4.0 37.0 4.0 39.0 55 23.702874516307354 31.0 4.0 37.0 4.0 39.0 56 23.09421641791045 30.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 1.2855701594973041 1 2 1.9868097877552415 1 3 2.343251462477639 1 4 2.1047925490723216 1 5 1.942444815526592 1 6 2.390522769655014 1 7 2.2881181934439283 1 8 1.9818562289806252 1 9 2.2466614832858056 1 10 1.016272045965927 1 11 -3.7545054536275515 1 12 -5.676829033422745 1 13 -18.371809566417017 1 14 -15.895578823424422 1 15 -10.526583220420957 1 16 -20.179209450829948 1 17 -19.497692035320274 1 18 -18.42833019468757 1 19 -5.864372867799929 1 20 -9.843339430542642 1 21 -8.4576395639073 1 22 -4.039284848900884 1 23 -3.8141161015953244 1 24 -3.1123873869802736 1 25 -4.410529592182527 1 26 1.8227035779137495 1 27 1.6926193582544968 1 28 1.0476288547700605 1 29 0.9666800981257779 1 30 1.9066479863180703 1 31 -0.5069222236507755 1 32 -10.010953194474265 1 33 0.9965959922152834 1 34 -0.011316763793047357 1 35 0.306096290834347 1 36 4.357830750181755 1 37 4.34516279207379 1 38 4.848424740623031 1 39 7.2873724059549865 1 40 8.892590878582105 1 41 -2.553236335487984 1 42 -8.397755338558127 1 43 -11.299983822100113 1 44 -14.16694660338749 1 45 -13.203302710684497 1 46 -17.54498487354177 1 47 -17.080933567465305 1 48 -16.143786439743756 1 49 -14.54440695032665 1 50 -15.535224211085595 1 51 -17.110275350088823 1 52 -16.6774200508695 1 53 -16.31179720209398 1 54 -16.985121744956544 1 55 -15.704636428030533 1 56 -15.072886400662586 2 1 1.1709868261639684 2 2 1.901988359183811 2 3 2.355156224382398 2 4 2.1226496919294675 2 5 2.134409101240877 2 6 2.423260864893109 2 7 2.3327610505867824 2 8 2.559237181361574 2 9 2.440113864238185 2 10 2.360022045965927 2 11 -3.241112596484694 2 12 -10.952126652470366 2 13 -12.309309566417017 2 14 -14.400043109138709 2 15 -6.156047506135241 2 16 -1.862245165115663 2 17 -0.8161444162726532 2 18 -9.077139718497097 2 19 -1.4789562011332613 2 20 -2.6007799067331163 2 21 3.9768842456165103 2 22 4.182441341575306 2 23 3.7067172317380113 2 24 4.1673745177816315 2 25 3.489768026865093 2 26 3.4640726255328005 2 27 3.829524120159256 2 28 3.05655742619863 2 29 3.233049145744829 2 30 3.1521837006037856 2 31 -0.17061269984125005 2 32 -9.798155575426645 2 33 0.16623884935813749 2 34 0.16279037906409854 2 35 -0.475153709165653 2 36 4.429259321610324 2 37 5.290103268264264 2 38 6.510627121575411 2 39 8.434693834526417 2 40 9.696162307153536 2 41 6.073251759750111 2 42 2.1632565662037777 2 43 -3.295519536385827 2 44 -10.681827555768443 2 45 -1.0589574725892597 2 46 -11.1610563021132 2 47 -11.091350234131971 2 48 -8.791107868315185 2 49 -11.818216474136173 2 50 -7.956355163466547 2 51 -7.570096778660252 2 52 -8.284562908012358 2 53 -9.478463868760647 2 54 -15.505955078289876 2 55 -9.607910237554343 2 56 -9.31544592447211 3 1 1.7709868261639699 3 2 2.997226454421906 3 3 2.904362573588749 3 4 3.2909036601834316 3 5 2.8820281488599235 3 6 3.0502449918772356 3 7 2.875618193443927 3 8 3.0290784512028495 3 9 2.9369392610635856 3 10 2.660022045965924 3 11 2.546188990816894 3 12 3.089143188799479 3 13 -4.264865121972573 3 14 -4.158773267868867 3 15 -0.7258887759765109 3 16 3.6520405491700494 3 17 3.6092524091241707 3 18 3.5577809164235425 3 19 1.5099326877556294 3 20 2.0865216805684668 3 21 3.422915991648253 3 22 3.10307626221023 3 23 3.5622727872935656 3 24 3.6403903907975064 3 25 2.4929426300396926 3 26 2.5942313556915266 3 27 2.4787304693656083 3 28 1.8406844103256148 3 29 3.0409856536813322 3 30 3.093453541873629 3 31 3.1055777763492216 3 32 3.2939079166368437 3 33 3.7979848811041705 3 34 3.631044347318067 3 35 3.9470685130565712 3 36 5.257830750181753 3 37 5.425023903184904 3 38 4.885230296178584 3 39 8.439455739288316 3 40 9.900924211915438 3 41 10.244680331178682 3 42 7.791827994775208 3 43 1.3409884001221073 3 44 2.7483311743902874 3 45 6.014058400426613 3 46 -2.6547070957639924 3 47 -4.691350234131971 3 48 -1.3403142175215343 3 49 -10.461073616993316 3 50 -2.5373075444189297 3 51 -4.530414238977713 3 52 -4.31630893975839 3 53 -3.834019424316203 3 54 -9.5281773005121 3 55 -5.1650530946972 3 56 -2.03608084510703 4 1 1.8512589350075075 4 2 3.167294481632794 4 3 3.348353503293964 4 4 3.1226496919294675 4 5 3.0221642032816973 4 6 3.0321044023080788 4 7 3.2307202342602537 4 8 2.9946113310214386 4 9 2.457120666959277 4 10 2.5130832704557236 4 11 3.7588874035153026 4 12 3.7281454563731735 4 13 4.568241453991146 4 14 4.4366915847388455 4 15 5.629666779579043 4 16 7.198979324680256 4 17 6.523991638149116 4 18 6.38544531551651 4 19 5.395193458730681 4 20 5.048879957212463 4 21 4.157156354460049 4 22 4.257271273548092 4 23 4.19991451064957 4 24 4.099347306897279 4 25 3.9523530608787 4 26 2.702167863628034 4 27 3.4043540521320494 4 28 3.240230895586386 4 29 3.1956341797584287 4 30 3.3256530883588837 4 31 4.1219043069614685 4 32 4.623613132056345 4 33 4.431544971807121 4 34 4.621974052533481 4 35 4.688111596956794 4 36 6.408851158345023 4 37 6.130239322686034 4 38 7.354164536541397 4 39 9.097959140648864 4 40 12.277794960214756 4 41 6.4474014196140566 4 42 9.462576294094937 4 43 8.857541688103968 4 44 13.70932890681659 4 45 10.86621259543795 4 46 9.849147779519456 4 47 11.024296024371427 4 48 9.535422743929711 4 49 7.348450192530493 4 50 11.057250278710324 4 51 9.582964445829543 4 52 9.470539132803967 4 53 9.167794634640714 4 54 2.725337438716924 4 55 10.232225816867427 4 56 7.4940778850516985 5 1 1.9461806246135822 5 2 3.1445132761273342 5 3 2.779297974105546 5 4 2.886769293258368 5 5 2.6510203969219432 5 6 3.362906490585246 5 7 2.591897263211372 5 8 2.6301120429341154 5 9 2.605673111192779 5 10 1.3251383250356952 5 11 4.948256174279422 5 12 5.550641896809811 5 13 5.760457875443446 5 14 6.397299083552319 5 15 7.048271430741831 5 16 6.572970781728191 5 17 6.4252730809587995 5 18 5.0513209681031235 5 19 3.819493411269839 5 20 4.119596615968987 5 21 3.6906162500461903 5 22 2.875686081331672 5 23 2.640825758869795 5 24 2.937031217670892 5 25 2.103278547352353 5 26 1.2769186941928226 5 27 1.8466891256963578 5 28 1.7641986222119215 5 29 1.3365928888234535 5 30 2.2402235677134215 5 31 2.2823219623957343 5 32 3.257215410176901 5 33 2.797468085238542 5 34 1.566444864113926 5 35 2.269032337345976 5 36 3.718295866460828 5 37 3.9883313967249556 5 38 5.152930554576521 5 39 7.668913103629404 5 40 6.372242041372804 5 41 3.5128973854422476 5 42 10.118959777720946 5 43 13.73271966627198 5 44 11.956599908240417 5 45 9.726203103268993 5 46 10.209375591574506 5 47 9.272990851139348 5 48 10.54444030444229 5 49 10.507918631068698 5 50 10.972769974960919 5 51 8.684056045260014 5 52 9.130719484014222 5 53 9.025412100231602 5 54 9.339006162020201 5 55 8.53882287429505 5 56 7.719991506314159 6 1 1.899668996706609 6 2 2.6328853691505927 6 3 2.756042160152056 6 4 3.351885572328136 6 5 2.8370669085498506 6 6 3.200115792910829 6 7 3.126780984141604 6 8 3.2347632057248106 6 9 2.8149754367741764 6 10 2.3949057668961586 6 11 4.994767802186402 6 12 7.643665152623765 6 13 6.737202061489956 6 14 8.025206060296505 6 15 7.373852826090673 6 16 7.805528921263079 6 17 7.378761453051819 6 18 6.02806515414964 6 19 4.586935271734951 6 20 4.677736150852709 6 21 3.481313924464793 6 22 3.4803372441223743 6 23 3.8501280844511854 6 24 2.3091242409266997 6 25 3.6846738961895653 6 26 1.8815698569835178 6 27 2.42808447453357 6 28 2.671175366397968 6 29 2.545895214404851 6 30 2.2169677537599313 6 31 3.073019636814344 6 32 4.97814564273504 6 33 3.541654131750164 6 34 3.6827239338813698 6 35 3.408567221066903 6 36 3.439226099018967 6 37 4.220889536259836 6 38 4.664558461553263 6 39 7.436354964094523 6 40 1.8373583204425685 6 41 4.047781106372479 6 42 6.793378382372104 6 43 13.825742922085933 6 44 12.375204559403208 6 45 8.400621707920156 6 46 10.837282568318692 6 47 11.691595502302139 6 48 10.590951932349267 6 49 13.135825607812887 6 50 11.34486299821673 6 51 11.47475371967862 6 52 11.665603204944453 6 53 9.839365588603695 6 54 11.92040151085741 6 55 10.887660083597375 6 56 9.789758948174622 7 1 2.194796349973494 7 2 3.211512168707621 7 3 2.950394319620493 7 4 3.6226496919294675 7 5 3.1582186250504023 7 6 3.613737055369299 7 7 3.4279991458248773 7 8 3.4163800385044354 7 9 3.3924948166191413 7 10 3.2885934745373575 7 11 5.020792165420069 7 12 7.785968585624875 7 13 7.904976147868698 7 14 8.457099748004147 7 15 7.867762017674284 7 16 8.137754834884337 7 17 7.279093678965442 7 18 7.922860281502903 7 19 6.449615227438166 7 20 5.351601045647833 7 21 4.6673604360927 7 22 5.063393722527685 7 23 4.087669612690391 7 24 4.905469755876872 7 25 4.156434693531757 7 26 4.559310720770895 7 27 4.662857453492592 7 28 4.270843140484345 7 29 4.518763431459114 7 30 4.43789798631807 7 31 5.3531968239682755 7 32 5.392320615049545 7 33 5.023381706500999 7 34 5.353266569540288 7 35 4.881989147977201 7 36 4.1911640835150905 7 37 4.6234366015976 7 38 5.415389026337316 7 39 6.577550977383556 7 40 1.934257545248773 7 41 -9.593414906916555 7 42 7.258494661441873 7 43 12.204480463614171 7 44 11.08007720613632 7 45 8.13151871788693 7 46 10.791324650267754 7 47 11.694364051582316 7 48 12.399368322161003 7 49 13.08654543062573 7 50 11.924597217485836 7 51 13.739427030863556 7 52 14.167818044368595 7 53 14.283440893144114 7 54 14.708330635995836 7 55 8.96351833387423 7 56 10.255982646956461 8 1 2.0159480664740457 8 2 3.2840481598482683 8 3 3.5699956485241486 8 4 3.8635134793048778 8 5 3.2556715597126384 8 6 3.3163948626782727 8 7 2.8244554027462527 8 8 3.1882515778178373 8 9 3.2335800879369714 8 10 3.3483941389891854 8 11 4.41337245334919 8 12 7.806455850298182 8 13 8.132550898699261 8 14 7.955438618436041 8 15 8.071527244695321 8 16 7.945063804984006 8 17 7.4252730809587995 8 18 6.7722512006612625 8 19 6.563679457781468 8 20 5.677736150852709 8 21 4.272011598883395 8 22 4.829174453424699 8 23 4.850128084451185 8 24 5.35563586883368 8 25 4.475371570608168 8 26 3.5327326476811933 8 27 4.032735637324265 8 28 3.0432683896537824 8 29 3.9179882376606585 8 30 3.147200311899468 8 31 2.6079033577445756 8 32 4.0711688985489936 8 33 2.2160727364013297 8 34 3.403654166439509 8 35 1.966706755950625 8 36 0.5555051687864072 8 37 1.5929825595156508 8 38 1.8738607871346602 8 39 4.157285196652662 8 40 -1.7207812144411498 8 41 -8.92896307967403 8 42 5.188727219581409 8 43 9.197835945341748 8 44 7.584506884984602 8 45 5.563412405594573 8 46 9.44193373110939 8 47 10.06368852555795 8 48 10.381649606767873 8 49 12.92652328223149 8 50 10.833235091239988 8 51 11.963125812701872 8 52 12.316765995642129 8 53 12.746342332789741 8 54 13.059936394578337 8 55 13.120218223132255 8 56 9.626968250500205 9 1 2.1622148963394068 9 2 3.172665050913139 9 3 3.3827251466881663 9 4 3.716634654335479 9 5 3.1644842892108045 9 6 3.354338559128699 9 7 2.659828719759716 9 8 2.764750965822728 9 9 3.1117930622331755 9 10 2.860022045965927 9 11 5.059639283214551 9 12 7.614289387629885 9 13 6.638058854635613 9 14 7.54356591341768 9 15 6.892824674315886 9 16 6.852040549170052 9 17 6.249018490995514 9 18 5.920354015838743 9 19 4.959640290094811 9 20 5.454357937878413 9 21 2.798939383461118 9 22 4.097228308993856 9 23 3.636541793141518 9 24 3.946823139335514 9 25 3.7917730393964177 9 26 2.284874630545332 9 27 2.8570930424650243 9 28 2.695655170559533 9 29 2.738061677073148 9 30 2.9642137757917553 9 31 3.937156723717642 9 32 5.082796805525735 9 33 4.2564644132679135 9 34 4.15151218357537 9 35 3.7616883960975045 9 36 4.5157254870238575 9 37 5.156018055231687 9 38 4.8928326353598734 9 39 5.032438195428671 9 40 0.6360119312136838 9 41 0.4516978750383309 9 42 6.469020977231345 9 43 11.26087144105778 9 44 9.84950076503356 9 45 7.360842026157609 9 46 10.03067302119507 9 47 10.54900064306101 9 48 10.426937244466771 9 49 11.837171997041771 9 50 9.943394209967035 9 51 8.884790439384862 9 52 10.12521152807787 9 53 9.898729113695492 9 54 10.932641412938196 9 55 10.675297782495782 9 56 9.997837283547941 10 1 2.0940637492408953 10 2 2.8433803005757525 10 3 3.208636077862252 10 4 3.5438951131748837 10 5 3.1692076360394097 10 6 2.9818689235011675 10 7 3.449977167802899 10 8 3.3248049469293406 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2.5222461254071256 115 38 3.754674740623031 115 39 5.089455739288319 115 40 8.267590878582105 115 41 6.728013664512016 115 42 1.3418279947752048 115 43 1.1270995112332187 115 44 -0.10444660338749046 115 45 2.4112806226488352 115 46 -3.5241515402084396 115 47 -9.091350234131971 115 48 -3.9042031064104243 115 49 -9.086073616993316 115 50 -1.7539742110855947 115 51 0.5251413165778445 115 52 0.9475799491304997 115 53 -0.020130535427313134 115 54 -0.17262174495654392 115 55 0.28494690530279954 115 56 2.3750302660040816 116 1 1.2959868261639684 116 2 2.3305597877552415 116 3 2.593251462477639 116 4 2.896459215738986 116 5 2.2653614821932564 116 6 1.9530227696550142 116 7 2.433951526777264 116 8 2.3985228956472895 116 9 2.5174948166191413 116 10 1.9016887126325912 116 11 -1.5149221202942194 116 12 -3.1664123667560773 116 13 -7.559309566417017 116 14 -9.197662156757755 116 15 -2.9119998870876245 116 16 -9.814626117496616 116 17 -8.810192035320274 116 18 -3.636663528020904 116 19 -4.687289534466597 116 20 -2.707922763875974 116 21 1.3340271027593644 116 22 2.116965151099116 116 23 1.6650505650713399 116 24 1.970945946353062 116 25 1.6207204078174726 116 26 1.8331202445804138 116 27 3.0259526915878325 116 28 2.943462188103396 116 29 1.7270967647924422 116 30 2.3545646529847346 116 31 1.7222444430158887 116 32 -4.042203194474265 116 33 2.5590959922152834 116 34 1.6449332362069526 116 35 2.024846290834347 116 36 3.399497416848419 116 37 4.022246125407126 116 38 3.4630080739563667 116 39 5.964455739288322 116 40 7.809257545248773 116 41 4.894680331178684 116 42 1.1751613281085405 116 43 -7.664567155433447 116 44 -0.9377799367208226 116 45 0.16128062264883525 116 46 -3.815818206875104 116 47 -7.674683567465305 116 48 -9.112536439743756 116 49 -4.79440695032665 116 50 -2.2539742110855947 116 51 0.31680798324450876 116 52 0.6559132824638318 116 53 -2.311797202093981 116 54 -2.922621744956544 116 55 -1.6317197613638648 116 56 0.2916969326707459 117 1 0.637653492830637 117 2 1.6638931210885772 117 3 2.0599181291443074 117 4 2.3131258824056573 117 5 1.8820281488599235 117 6 1.8946894363216842 117 7 1.808951526777264 117 8 2.0735228956472938 117 9 2.025828149952474 117 10 2.1266887126325926 117 11 -7.364922120294217 117 12 -8.333079033422745 117 13 -7.975976233083685 117 14 -7.714328823424424 117 15 -9.703666553754289 117 16 -13.681292784163283 117 17 -5.835192035320272 117 18 -1.753330194687571 117 19 -3.44562286779993 117 20 -3.5579227638759754 117 21 1.6673604360927001 117 22 2.1919651510991187 117 23 1.6733838984046727 117 24 0.12927927968639352 117 25 1.3373870744841412 117 26 2.2831202445804166 117 27 3.1009526915878283 117 28 2.4851288547700605 117 29 1.752096764792448 117 30 1.9378979863180703 117 31 -2.2277555569841105 117 32 -10.617203194474264 117 33 -1.0909040077847152 117 34 -0.9467334304597124 117 35 -1.3418203758323202 117 36 1.591164083515089 117 37 2.38057945874046 117 38 2.3963414072896967 117 39 5.239455739288321 117 40 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118 23 0.30816650710032434 118 24 -5.041735213067231 118 25 0.1315899730348633 118 26 1.720801404000703 118 27 1.524141097384934 118 28 0.789476680857014 118 29 1.0332561850823012 118 30 1.6335501602311169 118 31 -1.520509180172514 118 32 -8.808507542300351 118 33 -0.34307792082819333 118 34 0.17210714925042936 118 35 -0.7360232743830437 118 36 1.4230481414861025 118 37 1.9226084442477038 118 38 3.520979088449117 118 39 4.419165884215857 118 40 6.8980256611908 118 41 6.1519267079902775 118 42 2.2584946614418726 118 43 -1.4725381699261995 118 44 -1.3598813859961858 118 45 -0.6883570585105829 118 46 -5.797702264846119 118 47 -10.598596610943567 118 48 -8.840797309308973 118 49 -5.098754776413607 118 50 -5.264843776302985 118 51 -3.5545688283496943 118 52 -4.818724398695586 118 53 -3.9422319847026763 118 54 -7.303056527565239 118 55 -4.515777732378357 118 56 -0.8351146615321525 119 1 0.7265423817195256 119 2 1.7750042321996844 119 3 2.0376959069220817 119 4 2.4242369935167645 119 5 1.793139259971035 119 6 1.2169116585439 119 7 1.4200626378883712 119 8 1.3290784512028466 119 9 0.8369392610635842 119 10 1.860022045965927 119 11 -7.264922120294219 119 12 -8.944190144533856 119 13 0.19069043358298288 119 14 -2.0587732678688653 119 15 -9.036999887087624 119 16 -7.647959450829948 119 17 -10.879636479764716 119 18 -0.9977746391320146 119 19 -1.312289534466597 119 20 -0.8468116527648633 119 21 0.05624932498158586 119 22 -0.23025707112310556 119 23 0.8178283428491184 119 24 -4.348498498091384 119 25 0.42627596337302975 119 26 3.083120244580414 119 27 1.9009526915878325 119 28 2.596239965881175 119 29 2.740985653681335 119 30 3.215675764095849 119 31 1.8055777763492245 119 32 -7.972758750029822 119 33 1.7535404366597263 119 34 1.9643776806513955 119 35 1.8026240686121255 119 36 4.024497416848419 119 37 3.480579458740454 119 38 5.074119185067474 119 39 7.617233517066101 119 40 10.323146434137662 119 41 6.144680331178684 119 42 0.7584946614418726 119 43 1.9187661778998866 119 44 -0.6183354922763797 119 45 1.869613955982171 119 46 -9.621373762430661 119 47 -10.035794678576416 119 48 -11.251425328632646 119 49 -5.849962505882205 119 50 -7.615085322196705 119 51 -6.919303127866602 119 52 -6.538531161980611 119 53 -8.867352757649536 119 54 -10.172621744956544 119 55 -8.520608650252754 119 56 -5.291636400662586 120 1 0.004320159497304132 120 2 0.49722645442190583 120 3 1.0376959069220817 120 4 1.3686814379612073 120 5 0.6820281488599278 120 6 1.16135610298835 120 7 0.5311737489994854 120 8 1.2735228956472895 120 9 0.3369392610635842 120 10 0.6377998237437055 120 11 -10.931588786960884 120 12 -1.999745700089413 120 13 -3.0315317886392386 120 14 -3.5587732678688653 120 15 -11.148110998198735 120 16 -8.09240389527439 120 17 -9.824080924209163 120 18 -3.608885750243129 120 19 -1.7567339789110399 120 20 -2.680144986098199 120 21 -0.7215284527961856 120 22 0.10307626221023014 120 23 -1.5710605460397673 120 24 -4.1818318314247165 120 25 0.20405374115080832 120 26 0.24978691124708519 120 27 -0.8212695306343889 120 28 -2.6815378118966073 120 29 -0.5367921240964435 120 30 -2.3398797914597083 120 31 -9.083311112539665 120 32 -12.806092083363154 120 33 -8.690904007784717 120 34 -13.257844541570826 120 35 -9.53070926472121 120 36 -6.697724805373802 120 37 -6.408309430148432 120 38 -7.425880814932526 120 39 -6.771655371822792 120 40 -7.454631343640116 120 41 -5.966430779932429 120 42 -11.963727560780349 120 43 -8.803456044322335 120 44 -13.285002158943046 120 45 -14.185941599573386 120 46 -16.51026265131955 120 47 -16.035794678576416 120 48 -14.862536439743756 120 49 -14.572184728104428 120 50 -14.503974211085595 120 51 -15.919303127866602 120 52 -15.4274200508695 120 53 -16.756241646538427 120 54 -16.061510633845433 120 55 -16.409497539141643 120 56 -15.513858622884808 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 814.0 5 12.0 6 13.0 7 52.0 8 93.0 9 56.0 10 55.0 11 52.0 12 52.0 13 78.0 14 88.0 15 118.0 16 146.0 17 201.0 18 270.0 19 306.0 20 421.0 21 482.0 22 601.0 23 657.0 24 735.0 25 821.0 26 870.0 27 901.0 28 1052.0 29 1091.0 30 1269.0 31 1411.0 32 1697.0 33 1920.0 34 2407.0 35 2789.0 36 3002.0 37 2645.0 38 1398.0 39 369.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 100.0 0.0 0.0 2 0.0 0.0 0.0 100.0 3 100.0 0.0 0.0 0.0 4 0.0 0.0 100.0 0.0 5 0.0 0.0 100.0 0.0 6 100.0 0.0 0.0 0.0 7 99.37119955776672 0.5147871752349364 0.07946379215035931 0.03454947484798231 8 99.0153399668325 0.6771697070204533 0.1554726368159204 0.15201768933112217 9 94.89926391816705 2.892490582990635 1.188789439126378 1.0194560597159346 10 43.22484798231067 37.93877833056937 8.260779436152571 10.575594250967384 11 30.88032061912659 29.96130458817026 19.254422332780543 19.903952459922607 12 34.53911000552792 16.69776119402985 21.565782200110558 27.197346600331674 13 24.875621890547265 15.170674405749033 23.41763405196241 36.53606965174129 14 22.99557644456732 18.5547414984794 26.095521150124412 32.35416090682887 15 24.962801481020104 18.139035952801137 24.834769369182325 32.06339319699644 16 25.911714888174497 20.453524145321303 26.886515261502296 26.7482457050019 17 29.485761680951068 22.504838263754493 22.359690351119713 25.64970970417473 18 29.946099094741207 25.6132955566305 19.89841752470458 24.542187823923708 19 26.473840624784025 27.952864745317573 20.64413573847536 24.929158891423043 20 25.872675744798507 27.434851731526923 20.643533559134582 26.048938964539985 21 25.190892443768785 23.02111045848737 26.814773865874304 24.973223231869536 22 28.916453596848868 24.11719991707553 21.978439637896482 24.98790684817912 23 28.35820895522388 26.748203427307903 22.982310668877833 21.91127694859038 24 26.333609729132117 25.652985074626866 22.88902708678828 25.12437810945274 25 25.23148148148148 27.300995024875625 23.528192371475953 23.939331122166944 26 24.843344296347585 24.711788125324563 28.523455080491605 21.921412497836247 27 27.656412029035604 20.41479433114414 27.17248530936744 24.756308330452818 28 24.460830131200886 26.035933118703912 26.551736075051064 22.951500675044137 29 23.89921891200664 23.99944701734983 28.993571576691778 23.107762493951753 30 21.858471324368235 24.513430359180006 32.250146921561175 21.377951394890587 31 21.68265733781303 25.39226213155069 33.90599563575907 19.01908489487721 32 24.393530997304584 21.328357177413782 33.16746146934826 21.110650355933373 33 22.752293577981654 24.02977323870521 33.89302406093128 19.324909122381857 34 20.753346477119436 23.23683027221473 38.04780187471897 17.962021375946872 35 20.605117565698478 26.00968188105118 37.45850622406639 15.926694329183954 36 21.25030288345045 24.88490428882966 35.20024922981065 18.66454359790924 37 20.82871396895787 25.367239467849224 36.29088137472284 17.51316518847007 38 21.848390446521286 26.763586015922463 36.64935964001385 14.738663897542404 39 19.871039312209664 21.63211537128198 31.73403591485821 26.762809401650138 40 12.954213853956784 16.629611616758147 27.423052872924337 42.993121656360735 41 15.645476090896418 19.040150564617313 28.969747664854317 36.34462567963195 42 18.905627705627705 26.11948051948052 37.26060606060606 17.71428571428571 43 12.584020511399071 14.84304621994318 28.941168318203868 43.631764950453885 44 12.429280483148798 16.72625039047586 27.548505779042724 43.295963347332616 45 19.54054802103515 24.896208137282034 38.074314973706066 17.48892886797675 46 18.897610567447007 25.360489643486982 38.01307099138974 17.728828797676268 47 16.663211002833645 22.911051212938006 36.477987421383645 23.9477503628447 48 19.613603373194167 25.86230732010783 38.010644916015764 16.513444390682242 49 12.781225084064202 17.25309390924533 27.437861822719867 42.52781918397061 50 16.300093519448584 26.355166083613312 38.95950954244744 18.38523085449067 51 19.864747702444944 26.33258193168025 36.62909658401248 17.17357378186232 52 17.54069343699025 27.99777878041162 37.01107139138583 17.4504563912123 53 17.844033520326892 28.312209986841193 36.370247247039266 17.473509245792645 54 15.273406426175077 24.777781620724245 33.72185522083492 26.226956732265762 55 17.86060354374308 27.66472868217054 36.87707641196013 17.59759136212625 56 18.352436026178193 27.3728314692337 36.05041725821531 18.224315246372797 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 1.0 9 1.0 10 5.0 11 9.0 12 36.5 13 79.0 14 94.0 15 110.5 16 127.0 17 138.0 18 149.0 19 166.5 20 184.0 21 203.5 22 265.5 23 308.0 24 406.0 25 504.0 26 597.0 27 690.0 28 820.0 29 1079.5 30 1209.0 31 1222.0 32 1235.0 33 1208.5 34 1182.0 35 1123.0 36 1064.0 37 988.0 38 822.0 39 732.0 40 675.0 41 618.0 42 601.5 43 585.0 44 584.0 45 583.0 46 578.5 47 643.0 48 712.0 49 774.0 50 836.0 51 849.5 52 863.0 53 928.0 54 1086.5 55 1180.0 56 1278.5 57 1377.0 58 1415.5 59 1454.0 60 1638.5 61 1823.0 62 1722.0 63 1586.0 64 1551.0 65 1432.5 66 1314.0 67 1163.0 68 1012.0 69 874.5 70 737.0 71 642.5 72 449.0 73 350.0 74 309.5 75 269.0 76 211.5 77 154.0 78 120.0 79 62.5 80 39.0 81 29.5 82 20.0 83 12.0 84 4.0 85 3.0 86 2.0 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.02418463239358762 10 0.0 11 0.0 12 0.0 13 0.0 14 0.027639579878385848 15 0.1554726368159204 16 0.051824212271973466 17 0.027639579878385848 18 0.006909894969596462 19 0.020729684908789386 20 0.03454947484798231 21 0.003454947484798231 22 0.006909894969596462 23 0.0 24 0.0 25 0.0 26 0.20384190160309562 27 0.04836926478717524 28 0.1969320066334992 29 0.03454947484798231 30 0.05873410724156993 31 0.25221116639027086 32 0.020729684908789386 33 0.20384190160309562 34 0.11401326699834163 35 0.08291873963515754 36 0.1900221116639027 37 0.2763957987838585 38 0.18656716417910446 39 0.33858485351022666 40 0.5458817025981205 41 0.8706467661691543 42 0.23839137645107794 43 0.28330569375345493 44 0.4595080154781647 45 0.13819789939192925 46 0.08637368711995577 47 0.020729684908789386 48 0.03454947484798231 49 0.3351299060254284 50 0.25221116639027086 51 0.3765892758430072 52 0.4525981205085683 53 0.22802653399668327 54 0.10710337202874515 55 0.16583747927031509 56 0.22457158651188502 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 28944.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.89275843007186 #Duplication Level Percentage of deduplicated Percentage of total 1 94.56439105975369 85.95218352681039 2 3.9037555116314433 7.0964621337755664 3 0.8096396533373879 2.20771144278607 4 0.24327200851452033 0.8844665561083471 5 0.12923825452333892 0.5873410724156993 6 0.09502812832598449 0.5182421227197347 7 0.04561350159647256 0.29021558872305137 8 0.03421012619735442 0.24875621890547264 9 0.04181237646343318 0.3420398009950249 >10 0.1330393796563783 1.8725815367606413 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACGTTGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 47 0.16238253178551687 No Hit ACGTTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA 34 0.11746821448313986 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.010364842454394693 0.0 0.0 0.0 0.0 11 0.017274737423991156 0.0 0.0 0.0 0.0 12 0.017274737423991156 0.0 0.0 0.0 0.0 13 0.017274737423991156 0.0 0.0 0.0 0.0 14 0.020729684908789386 0.0 0.0 0.0 0.0 15 0.020729684908789386 0.0 0.0 0.0 0.0 16 0.020729684908789386 0.0 0.0 0.0 0.0 17 0.027639579878385848 0.0 0.0 0.0 0.0 18 0.03454947484798231 0.0 0.0 0.0 0.0 19 0.04145936981757877 0.0 0.0 0.0 0.0 20 0.044914317302377 0.0 0.0 0.0 0.0 21 0.044914317302377 0.0 0.0 0.0 0.0 22 0.04836926478717524 0.0 0.0 0.0 0.0 23 0.055279159756771695 0.0 0.0 0.0 0.0 24 0.055279159756771695 0.0 0.0 0.0 0.0 25 0.055279159756771695 0.0 0.0 0.0 0.0 26 0.06218905472636816 0.0 0.0 0.0 0.0 27 0.06909894969596463 0.0 0.0 0.0 0.0 28 0.06909894969596463 0.0 0.0 0.0 0.0 29 0.07255389718076286 0.0 0.0 0.0 0.0 30 0.07255389718076286 0.0 0.0 0.0 0.0 31 0.07600884466556108 0.0 0.0 0.0 0.0 32 0.07600884466556108 0.0 0.0 0.0 0.0 33 0.07600884466556108 0.0 0.0 0.0 0.0 34 0.07600884466556108 0.0 0.0 0.0 0.0 35 0.07600884466556108 0.0 0.0 0.0 0.0 36 0.07600884466556108 0.0 0.0 0.0 0.0 37 0.07600884466556108 0.0 0.0 0.0 0.0 38 0.07600884466556108 0.0 0.0 0.0 0.0 39 0.07600884466556108 0.0 0.0 0.0 0.0 40 0.07600884466556108 0.0 0.0 0.0 0.0 41 0.07600884466556108 0.0 0.0 0.0 0.0 42 0.07946379215035931 0.0 0.0 0.0 0.0 43 0.08291873963515754 0.0 0.0 0.0 0.0 44 0.08291873963515754 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGTTT 15 0.008092545 50.394047 38 CAACCTG 15 0.008092545 50.394047 41 CCTTCTT 15 0.008092545 50.394047 39 GGCTGTT 15 0.008148856 50.305946 42 ACAACAC 20 4.0279632E-4 50.21815 43 CCAACAC 30 9.822688E-7 50.218147 43 GCTGTTC 15 0.0082054585 50.218147 43 TTTTCGT 15 0.008262353 50.13066 36 GCGTGCA 15 0.008262353 50.13066 47 TACAAAC 15 0.008262353 50.13066 37 CCTCAGT 15 0.008262353 50.13066 48 ATCCCAA 15 0.00831954 50.04348 26 TCCCAAA 15 0.00831954 50.04348 27 AAAGGTG 15 0.00831954 50.04348 33 AAGGTGC 15 0.00831954 50.04348 34 GTGAACG 15 0.00831954 50.04348 26 GAAAGGT 15 0.00831954 50.04348 32 AGGACAA 30 1.0307995E-6 49.870018 18 CCCGGAG 15 0.008434798 49.870018 13 CCTTGGA 15 0.008434798 49.870018 20 >>END_MODULE