FastQCFastQC Report
Fri 17 Jun 2016
SRR1526366_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526366_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34618
Sequences flagged as poor quality0
Sequence length56
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTACCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1080.3119764284476284No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC690.19931827373042926No Hit
TCTACCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC610.17620890866023456No Hit
TCTACCGGGCTCGTCTTAATCAGCAACATCTTTTGATACCCAAAAAAAAAAAAAAA520.15021087295626553No Hit
TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC430.12421283725229648No Hit
TCTACCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGACAACCTGCGTGCAATC410.11843549598474783No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC203.8217002E-450.78254739
ACAAACC150.00786303850.78254338
GCGAACC150.00786303850.78254339
ACCAACC150.00786303850.78254338
GGCGAAC150.00786303850.78254338
CCACCAG150.00786303850.78254340
CGAACCT150.00786303850.78254340
TCCGACC150.00786303850.78254338
GCCCTTG150.00786303850.78254338
AGAGCCC203.877988E-450.63274835
TACCCAA203.877988E-450.63274837
CGGCGAA150.00795560450.63274437
CCGGCGA150.00795560450.63274436
TAGAGCC309.524938E-750.4838234
TGTGAAA150.008285982550.1153349
ACAAAAT150.008285982550.1153348
CTGCAAT150.008285982550.1153349
GCCTGCA150.008285982550.1153347
ATTCAAG150.008285982550.1153333
CGCGCAA150.008285982550.1153347