##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526366_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 34618 Sequences flagged as poor quality 0 Sequence length 56 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.49153619504304 39.0 38.0 39.0 33.0 40.0 2 36.68051302790456 39.0 38.0 39.0 30.0 40.0 3 36.434022762724595 39.0 38.0 39.0 29.0 40.0 4 34.5550291755734 39.0 35.0 39.0 18.0 40.0 5 36.93679588653302 39.0 38.0 39.0 33.0 40.0 6 37.09188861286036 39.0 38.0 39.0 33.0 40.0 7 36.59128199202727 38.0 36.0 39.0 31.0 40.0 8 36.603501068808136 38.0 36.0 39.0 31.0 40.0 9 36.53056213530533 38.0 36.0 39.0 31.0 40.0 10 36.21249061182044 38.0 36.0 39.0 30.0 40.0 11 35.16037899358715 38.0 34.0 39.0 28.0 40.0 12 32.97706395516783 37.0 31.0 39.0 19.0 40.0 13 32.34493616037899 36.0 30.0 39.0 16.0 40.0 14 32.005835114680224 36.0 29.0 39.0 15.0 40.0 15 32.36160378993587 36.0 30.0 39.0 17.0 40.0 16 32.06759489283032 36.0 29.0 38.0 17.0 40.0 17 32.117077820786875 36.0 29.0 38.0 18.0 40.0 18 32.08567797099775 35.0 29.0 38.0 18.0 40.0 19 33.167658443584266 36.0 30.0 38.0 23.0 40.0 20 33.55563579640649 36.0 31.0 38.0 24.0 40.0 21 35.06054653648391 38.0 35.0 39.0 27.0 40.0 22 34.71532150904154 38.0 34.0 39.0 26.0 40.0 23 34.94988156450402 38.0 34.0 39.0 27.0 40.0 24 34.51008146051187 38.0 33.0 39.0 25.0 40.0 25 34.83199491593968 38.0 34.0 39.0 27.0 40.0 26 35.29392223698654 38.0 35.0 39.0 28.0 40.0 27 35.31139869432087 38.0 35.0 39.0 28.0 40.0 28 35.30293488936392 38.0 35.0 39.0 29.0 40.0 29 35.13634525391415 38.0 35.0 39.0 28.0 40.0 30 34.96449823791092 38.0 35.0 39.0 27.0 40.0 31 34.75717834652493 38.0 35.0 39.0 27.0 40.0 32 33.72491189554567 38.0 33.0 39.0 20.0 40.0 33 34.97440637818476 38.0 35.0 39.0 28.0 40.0 34 34.7373331792709 38.0 35.0 39.0 27.0 40.0 35 34.74426598879196 38.0 35.0 39.0 27.0 40.0 36 32.37032757524987 35.0 31.0 38.0 22.0 39.0 37 32.08512912357733 35.0 31.0 38.0 20.0 39.0 38 31.21592812987463 35.0 30.0 38.0 12.0 39.0 39 29.290224738575308 34.0 27.0 38.0 4.0 39.0 40 24.94355537581605 31.0 11.0 37.0 4.0 39.0 41 21.216881391183776 24.0 8.0 32.0 4.0 36.0 42 25.347131550060663 30.0 19.0 34.0 4.0 37.0 43 21.43023860419435 26.0 7.0 33.0 4.0 36.0 44 22.590819804725864 28.0 8.0 33.0 4.0 37.0 45 24.9910451210353 30.0 18.0 34.0 4.0 37.0 46 25.162863250332197 30.0 11.0 36.0 4.0 38.0 47 24.262984574498816 30.0 5.0 36.0 4.0 38.0 48 24.284505170720433 30.0 4.0 35.0 4.0 38.0 49 21.661852215610377 26.0 4.0 35.0 4.0 38.0 50 24.019469640071637 29.0 4.0 35.0 4.0 38.0 51 25.515454387890692 32.0 4.0 38.0 4.0 39.0 52 25.2611358252932 32.0 4.0 38.0 4.0 39.0 53 24.816627188168006 31.0 4.0 38.0 4.0 39.0 54 23.985874400600842 31.0 4.0 38.0 4.0 39.0 55 24.190652261829108 31.0 4.0 38.0 4.0 39.0 56 22.938240221849906 29.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.9714442428142576 1 2 2.3210565348584 1 3 2.950668716308307 1 4 4.612970913920975 1 5 1.674366807497968 1 6 1.575060735487611 1 7 1.8233402892937605 1 8 2.064264305664139 1 9 1.9323566102346916 1 10 2.1185106447354727 1 11 -3.3163601789670594 1 12 -7.715295476721838 1 13 -21.902383336845595 1 14 -19.33948755837387 1 15 -10.591006548991022 1 16 -21.153250156858423 1 17 -21.07805763973324 1 18 -20.551540551472925 1 19 -5.886032387037517 1 20 -11.068581068315435 1 21 -7.662772461088178 1 22 -2.115028435646966 1 23 -2.3234263720481465 1 24 -3.1448223782419618 1 25 -3.511268297421225 1 26 2.3120756500251147 1 27 2.390445490729199 1 28 2.4293703638115147 1 29 1.9546667036527055 1 30 2.6708553547280687 1 31 0.24088947254129778 1 32 -12.73887050753683 1 33 0.7339196849967493 1 34 0.31748788683412954 1 35 0.25281007467788186 1 36 3.5068101675382266 1 37 4.005661066338746 1 38 4.7081636993853095 1 39 7.077183013250785 1 40 8.515891751354765 1 41 -2.619689261025542 1 42 -7.508809559526096 1 43 -13.185688835150843 1 44 -15.45111145518226 1 45 -14.79487294926862 1 46 -17.93057923664382 1 47 -17.19327947177255 1 48 -15.940569327456139 1 49 -13.854484538426815 1 50 -15.739530489135774 1 51 -16.576608427477474 1 52 -16.187907525854428 1 53 -16.314578234448845 1 54 -17.143758208766304 1 55 -15.807451360619332 1 56 -14.55352855379196 2 1 0.8941715155415295 2 2 2.0960565348583984 2 3 2.959759625399215 2 4 4.444789095739154 2 5 1.2039122620434242 2 6 1.4455152809421605 2 7 1.7256130165664842 2 8 1.9415370329368642 2 9 1.9437202465983248 2 10 1.9548742810991087 2 11 -3.875451088057968 2 12 -13.124386385812748 2 13 -13.802383336845597 2 14 -15.391760285646598 2 15 -8.136461094445568 2 16 -3.475977429585697 2 17 -2.7189667306423324 2 18 -11.09017691510929 2 19 -2.2519414779466054 2 20 -3.5958537955881624 2 21 2.273591175275456 2 22 2.5963352007166662 2 23 2.27884635522458 2 24 2.505177621758037 2 25 1.7932771571242299 2 26 2.0416211045705666 2 27 2.617718218001926 2 28 1.2339158183569694 2 29 2.843303067289071 2 30 1.9208553547280687 2 31 -3.272746891095064 2 32 -9.377506871173193 2 33 -3.7228984968214327 2 34 -3.164330294984051 2 35 -2.4585535616857577 2 36 3.2681738039018597 2 37 3.9033883390660193 2 38 4.564981881203487 2 39 7.127183013250782 2 40 9.665891751354764 2 41 6.805310738974459 2 42 2.9320995313829954 2 43 -3.581143380605388 2 44 -7.842020546091353 2 45 0.6460361416404687 2 46 -10.278306509371092 2 47 -9.579643108136185 2 48 -7.270114782001594 2 49 -11.136302720244997 2 50 -6.925894125499411 2 51 -5.899335700204748 2 52 -6.292452980399879 2 53 -6.453214598085207 2 54 -12.923303663311758 2 55 -5.546087724255695 2 56 -3.4853467356101433 3 1 1.4547775761475918 3 2 2.8899161839812066 3 3 3.4541774882381304 3 4 5.026128808657816 3 5 2.328314175919026 3 6 2.193920384610422 3 7 2.3009718682411275 3 8 2.4423344811027334 3 9 2.868760119006623 3 10 2.603598364033722 3 11 1.0292538561206612 3 12 2.1662834706465794 3 13 -4.771681582459632 3 14 -5.769750716268607 3 15 -2.1567960226752305 3 16 4.037977913317015 3 17 3.2666792023720177 3 18 3.4664419046674233 3 19 1.878441297173012 3 20 1.4559803351933382 3 21 3.7995082406662064 3 22 4.144182090668821 3 23 3.8235034525132576 3 24 3.7332477971966327 3 25 3.5624159131050916 3 26 2.4993563517794968 3 27 2.675533210027446 3 28 3.1960370304781804 3 29 2.8173860018983206 3 30 3.4822588635000002 3 31 2.744398244471121 3 32 2.1703400187789583 3 33 3.1168144218388534 3 34 2.9692422727990433 3 35 2.3707925308182283 3 36 5.421283851748754 3 37 5.4420645751106775 3 38 6.291058436227409 3 39 8.447358451847276 3 40 11.5430847338109 3 41 11.629872142483233 3 42 9.06048868608794 3 43 1.8195743227438932 3 44 4.463800825519492 3 45 9.191091963012077 3 46 -0.8652283594508354 3 47 -4.346788243702374 3 48 -0.6796044151754366 3 49 -8.643081029654885 3 50 -0.42110943650419586 3 51 -1.424854041512564 3 52 -0.9431706837491625 3 53 -0.2869466555014739 3 54 -5.363495050871567 3 55 -0.6473636413210855 3 56 2.5517346041027764 4 1 1.7020894041045764 4 2 2.5557339542132382 4 3 3.389378393727661 4 4 4.82748704295323 4 5 1.8945280978205545 4 6 1.8597381548424536 4 7 2.054791902196982 4 8 2.2626514024383297 4 9 2.4686469328153393 4 10 1.7459299995741873 4 11 2.8965430468393905 4 12 1.9701883942459055 4 13 4.125036017993114 4 14 1.9968027642067767 4 15 5.642864418750914 4 16 6.288685327012544 4 17 5.307426231234501 4 18 5.25733041626901 4 19 4.0567095484463565 4 20 4.292709254265212 4 21 2.613033990524727 4 22 3.137390919191745 4 23 2.922541369887334 4 24 3.2309840733709407 4 25 2.3467962187078086 4 26 1.780624037121889 4 27 1.9307680713743594 4 28 2.432596170263132 4 29 2.4070860584914158 4 30 2.1305327740829085 4 31 2.7118572144767796 4 32 2.7280649763341387 4 33 1.7331132333838468 4 34 2.912649177156716 4 35 2.2584552359681993 4 36 4.6156811352801626 4 37 4.751628808274226 4 38 6.391228215514339 4 39 8.82879591647659 4 40 11.23040788038702 4 41 7.724665577684135 4 42 9.679900117893258 4 43 9.79495632613948 4 44 12.240824028688706 4 45 10.353514147505571 4 46 9.52667882787231 4 47 9.814785044356483 4 48 8.773140349963217 4 49 4.601967074476409 4 50 9.178211446348097 4 51 6.034681895103169 4 52 6.58144731285525 4 53 5.38622821716406 4 54 1.063499855749825 4 55 5.172387349058088 4 56 6.80131015588546 5 1 2.3436664650364776 5 2 3.5127232015250627 5 3 3.9395576051971943 5 4 5.800007950958012 5 5 2.9345519926831543 5 6 2.742653328080209 5 7 3.2668588078122767 5 8 3.0475976389974733 5 9 2.992541795419875 5 10 3.4018439780688112 5 11 4.228084265477385 5 12 6.31248230105594 5 13 6.892061107598845 5 14 6.613290219403908 5 15 7.047882339897864 5 16 7.435638732030462 5 17 7.408979397303796 5 18 6.717903892971517 5 19 5.959337983332858 5 20 5.594381894647526 5 21 4.368709020393304 5 22 3.941453045834514 5 23 4.496018072396296 5 24 4.616288732869151 5 25 3.9591020729491504 5 26 3.031520094469556 5 27 3.3071121573958635 5 28 3.010851845292997 5 29 3.674111148097147 5 30 3.7541886880614044 5 31 3.137185768837597 5 32 4.671314677648354 5 33 3.8292900553671245 5 34 3.582302701648949 5 35 3.0296619265297338 5 36 4.298476834204891 5 37 5.602883288560967 5 38 6.207237773459383 5 39 8.321627457695225 5 40 5.814039899502912 5 41 3.323829257492978 5 42 9.656005255288722 5 43 13.862459312997302 5 44 12.572962618891815 5 45 10.675497421101753 5 46 12.142568911504327 5 47 12.760424231931154 5 48 12.989986228099418 5 49 14.091811757869483 5 50 13.006765807160523 5 51 11.632650831781785 5 52 12.365796177849276 5 53 12.03079213592153 5 54 12.253464013455918 5 55 11.76199308382511 5 56 9.83536033509693 6 1 1.9138970730029357 6 2 3.1951131386319815 6 3 3.7676498483837833 6 4 5.196933178071916 6 5 2.409744165988535 6 6 2.3670418675630884 6 7 2.2294723647654564 6 8 2.4406794000037593 6 9 2.7035830253290314 6 10 2.5685106447354755 6 11 3.538356802165019 6 12 5.685647919504579 6 13 5.721201568814781 6 14 6.410984139739334 6 15 6.196729300065581 6 16 6.829768711066102 6 17 6.40967820932336 6 18 5.141383976828958 6 19 4.843684594094562 6 20 5.084249120363808 6 21 2.3589256521193676 6 22 3.3844998662398282 6 23 2.7119509864424174 6 24 2.599517244399543 6 25 1.8811845327674561 6 26 2.1908492349307735 6 27 1.9989360567669365 6 28 1.3218231940001957 6 29 1.6541950055394992 6 30 2.3170817698224084 6 31 1.3408894725412992 6 32 3.825752133972607 6 33 1.0532593076382568 6 34 1.5316388302303565 6 35 1.4042251690175007 6 36 1.8016214882929447 6 37 2.0127365380368616 6 38 3.152031623913608 6 39 4.5469943340055 6 40 -0.12656107883391599 6 41 1.7675748899178565 6 42 7.869492327266357 6 43 11.374688523339724 6 44 10.178133827836607 6 45 7.597579880920058 6 46 9.97555283882788 6 47 10.679362037661413 6 48 11.12310991782688 6 49 12.342685272893938 6 50 11.040658190109507 6 51 10.55263685554139 6 52 10.426243417541798 6 53 9.735893463664361 6 54 11.17275122519596 6 55 9.977925997871235 6 56 11.33326389903823 7 1 1.7614442428142567 7 2 2.7660565348584 7 3 3.5506687163083086 7 4 5.302970913920973 7 5 2.3493668074979723 7 6 2.190060735487613 7 7 2.348340289293759 7 8 2.554264305664141 7 9 2.5073566102346945 7 10 1.8485106447354767 7 11 3.1436398210329415 7 12 5.774704523278164 7 13 5.7476166631544 7 14 7.11551244162613 7 15 6.338993451008978 7 16 7.126749843141575 7 17 5.25194236026676 7 18 4.8934594485270715 7 19 4.708967612962482 7 20 4.651418931684567 7 21 2.2472275389118224 7 22 2.8399715643530357 7 23 1.5715736279518566 7 24 3.085177621758035 7 25 1.8287317025787786 7 26 1.7870756500251161 7 27 1.860445490729198 7 28 2.1693703638115167 7 29 2.489666703652702 7 30 1.3808553547280695 7 31 1.7408894725412978 7 32 3.306129492463171 7 33 2.8589196849967493 7 34 2.127487886834132 7 35 1.5378100746778784 7 36 0.41181016753822774 7 37 0.9006610663387491 7 38 1.9731636993853066 7 39 3.3171830132507836 7 40 -2.4941082486452366 7 41 -8.334689261025542 7 42 4.261190440473907 7 43 9.534311164849157 7 44 7.75888854481774 7 45 4.74512705073138 7 46 7.2144207633561805 7 47 8.476720528227453 7 48 8.17443067254386 7 49 11.695515461573184 7 50 7.170469510864226 7 51 8.893391572522528 7 52 9.002092474145574 7 53 10.300421765551157 7 54 10.271241791233695 7 55 9.057548639380666 7 56 9.226471446208041 8 1 1.7292873800691595 8 2 2.8362526132897727 8 3 3.6389040104259536 8 4 4.861010129607251 8 5 2.2003471996548356 8 6 1.9833940688209495 8 7 2.2483402892937576 8 8 2.3123035213504153 8 9 2.7583370023915563 8 10 2.176353781990379 8 11 3.665992762209413 8 12 5.7503907977879685 8 13 5.869185290605383 8 14 6.982571265155542 8 15 7.041346392185449 8 16 7.272240039220009 8 17 6.881746281835383 8 18 6.391106507350603 8 19 5.46151663257033 8 20 5.200046382664958 8 21 2.887227538911823 8 22 3.8325205839608785 8 23 3.8162795103047955 8 24 3.348314876659998 8 25 3.343633663363093 8 26 1.8811932970839393 8 27 3.0522101966115542 8 28 1.9999585991056321 8 29 3.5728039585546654 8 30 2.2737965311986557 8 31 3.6350071196001252 8 32 4.858678512071016 8 33 2.8804883124477314 8 34 3.306703573108642 8 35 3.3738885060504273 8 36 1.2200454616558716 8 37 2.3316414584956107 8 38 2.623359777816681 8 39 4.51443791521157 8 40 -4.726265111390333 8 41 -9.724101025731423 8 42 5.43922965616018 8 43 8.831958223672686 8 44 7.440065015405974 8 45 5.530617246809811 8 46 9.523832528062066 8 47 10.29201464587451 8 48 8.855999299994842 8 49 12.481789971377108 8 50 9.426155785374029 8 51 9.3635876509539 8 52 10.240523846694593 8 53 10.738853138100175 8 54 9.844967281429774 8 55 9.964607462910081 8 56 9.48568713248255 9 1 2.185274030048298 9 2 3.494992705071162 9 3 4.050668716308309 9 4 5.656587935197571 9 5 2.557877445795846 9 6 2.735167118466336 9 7 2.7377019914214173 9 8 3.0227749439620126 9 9 3.24352682300065 9 10 2.8663829851610103 9 11 4.017682374224428 9 12 5.938959842427099 9 13 6.671020918473548 9 14 7.271682654392087 9 15 6.8611211105834435 9 16 7.716962609099021 9 17 6.840027466649737 9 18 6.50750200171856 9 19 5.4293931448773805 9 20 5.4824827614718 9 21 3.738291368699059 9 22 3.502524755842394 9 23 4.200509798164617 9 24 3.8668797494176133 9 25 3.7057529791745196 9 26 2.991330969174051 9 27 3.3851263417930255 9 28 3.1251150446625786 9 29 3.538177341950579 9 30 3.580429822813173 9 31 3.830251174668959 9 32 3.847831620122747 9 33 3.0653026637201535 9 34 3.0121687378979587 9 35 3.028873904465115 9 36 2.9296825079637614 9 37 3.1359802152749197 9 38 3.8565679547044525 9 39 4.100587268569932 9 40 0.5382321768866767 9 41 -5.067029686557454 9 42 5.022041504303694 9 43 9.650481377615115 9 44 9.243569395881568 9 45 5.698744072007976 9 46 9.739101614420008 9 47 10.843954570780646 9 48 9.764217906586413 9 49 13.391260142424247 9 50 10.082809936396139 9 51 8.937221359756567 9 52 9.39613502733706 9 53 8.937017510232007 9 54 9.467412003999655 9 55 9.296272043635987 9 56 9.340088467484637 10 1 1.6769997983698133 10 2 2.7238343126361784 10 3 3.58400204964164 10 4 4.896304247254307 10 5 2.2160334741646395 10 6 1.6056162910431695 10 7 2.359451400404872 10 8 2.5253754167752476 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0.1257687396158147 115 26 1.7722608352102966 115 27 1.6774825277662373 115 28 2.7886296230707757 115 29 2.6185555925415898 115 30 2.8467812806539925 115 31 3.0075561392079635 115 32 -5.106463100129421 115 33 2.3663270924041555 115 34 1.6748952942415372 115 35 2.3629952598630624 115 36 5.0021805379086 115 37 5.584364770042448 115 38 7.170200736422345 115 39 9.562368198435966 115 40 10.332558418021428 115 41 8.5460514797152 115 42 3.600449699733165 115 43 2.269866720404714 115 44 -4.204815158885964 115 45 2.842164087768417 115 46 -2.190764421829005 115 47 -8.239575768068844 115 48 -6.324828586715398 115 49 -8.426706760649036 115 50 -2.3450860446913318 115 51 1.0956137947447466 115 52 1.717648029701131 115 53 1.6974588025881943 115 54 1.383093643085548 115 55 1.873104194936225 115 56 3.890915890652483 116 1 0.8251479465179585 116 2 2.0127232015250627 116 3 2.4951131607527515 116 4 0.3926005435505999 116 5 1.304922363053528 116 6 1.4648755503024304 116 7 1.989081030034498 116 8 1.91796800936784 116 9 1.7147640176420964 116 10 2.1611032373280707 116 11 -1.771915734522615 116 12 -4.020851032277395 116 13 -7.644975929438189 116 14 -12.368191262077573 116 15 -6.359525067509541 116 16 -11.527324230932496 116 17 -9.702131713807315 116 18 -5.059873884806258 116 19 -5.4295509055560345 116 20 -6.146358846093214 116 21 1.405746057430342 116 22 2.1636752680567355 116 23 1.5330551094333345 116 24 1.653325769906182 116 25 1.3479909618380361 116 26 2.475964538913999 116 27 2.529334379618085 116 28 2.862703697144852 116 29 2.4333704073564064 116 30 2.550484984357695 116 31 1.0445931762450016 116 32 -9.217574211240532 116 33 1.6626233887004531 116 34 1.1193397386859871 116 35 1.4370693339371385 116 36 4.261439797167853 116 37 3.399179584857265 116 38 5.688719254940864 116 39 6.5253311613989275 116 40 9.443669529132542 116 41 6.02753296119668 116 42 1.5263756256590923 116 43 -10.137540687002694 116 44 -3.427037381108189 116 45 0.6940159396202681 116 46 -3.0426162736808564 116 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0.1742228058746349 119 32 -8.51387050753683 119 33 0.875586351663415 119 34 -0.5241787798325319 119 35 -0.4055232586554567 119 36 3.3401435008715623 119 37 3.0473277330054103 119 38 3.9664970327186424 119 39 5.918849679917447 119 40 8.957558418021428 119 41 7.721977405641127 119 42 2.9661904404739055 119 43 -5.702355501817509 119 44 -5.792778121848928 119 45 0.763460384064711 119 46 -3.6305792366438183 119 47 -12.193279471772549 119 48 -8.648902660789473 119 49 -8.204484538426815 119 50 -3.9561971558024425 119 51 -3.4182750941441427 119 52 -6.046240859187758 119 53 -12.297911567782178 119 54 -11.26042487543297 119 55 -12.307451360619332 119 56 -9.67852855379196 120 1 0.4014442428142573 120 2 1.5460565348584012 120 3 2.2906687163083106 120 4 3.4429709139209734 120 5 0.9493668074979738 120 6 -0.6699392645123865 120 7 0.16834028929375933 120 8 -0.025735694335864423 120 9 0.2673566102346925 120 10 0.1285106447354778 120 11 -8.81636017896706 120 12 -1.7852954767218385 120 13 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120 51 -6.886608427477476 120 52 -6.957907525854425 120 53 -10.019578234448844 120 54 -11.108758208766304 120 55 -8.882451360619331 120 56 -4.95352855379196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 304.0 5 15.0 6 30.0 7 28.0 8 71.0 9 74.0 10 50.0 11 53.0 12 42.0 13 56.0 14 79.0 15 138.0 16 182.0 17 246.0 18 319.0 19 399.0 20 443.0 21 590.0 22 730.0 23 911.0 24 1002.0 25 1091.0 26 1121.0 27 1156.0 28 1264.0 29 1301.0 30 1436.0 31 1679.0 32 1986.0 33 2441.0 34 2904.0 35 3414.0 36 3778.0 37 3173.0 38 1629.0 39 483.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 100.0 0.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 99.24316829395113 0.4910740077416373 0.08377144837945577 0.18198624992778323 8 98.64232480212605 1.1381362297070887 0.12132416661852215 0.09821480154832746 9 93.74711049468331 3.843042071197411 1.2135922330097086 1.19625520110957 10 37.731815818360396 38.994164885319776 9.356404182795078 13.917615113524755 11 27.59258189381247 31.472066554971402 19.634294297764168 21.30105725345196 12 34.53694609740597 15.529493327170835 19.90871800797273 30.02484256745046 13 28.43607371887457 14.740886244150442 23.929747530186606 32.89329250678838 14 21.11239526148512 19.708176827506502 25.036116729268997 34.143311181739385 15 24.751902323293695 19.61056621242369 25.51283164077192 30.124699823510692 16 25.24058607635176 18.876975984741208 26.740456029824006 29.141981909083025 17 26.90107477175546 24.280596324973995 21.229631341731192 27.588697561539348 18 28.98127816942101 26.730613660002312 20.42644169652144 23.86166647405524 19 25.235481074833864 27.74920543195608 19.676394105749782 27.33891938746027 20 24.07016732653238 25.931277634887152 21.948963962662198 28.049591075918272 21 23.598913797088052 22.03605269239658 25.96487173561359 28.40016177490178 22 25.29608873996187 23.620659772372754 24.825235426656654 26.258016061008725 23 26.327344156219308 25.729389335028017 25.472297648622106 22.47096886013057 24 22.427638800623953 27.167947310647637 22.53740828470738 27.867005604021028 25 24.839678779825526 27.63591195331908 22.65295511005835 24.871454156797043 26 21.93406466178471 27.601377753335843 27.375611450403774 23.08894613447567 27 22.525646582863747 20.135818523334777 30.353995087415115 26.98453980638636 28 19.7516211208893 24.507874015748033 32.29214914312182 23.44835572024085 29 19.704632814080515 23.52244154793214 33.69555793185168 23.07736770613566 30 18.26614651061985 25.964456003467706 37.402109521745416 18.36728796416703 31 18.250014481839774 24.407692753287378 39.72658286508718 17.61570989978567 32 22.078072177757228 18.839030310034964 39.17478112629663 19.90811638591118 33 20.877370783263355 20.324308672361372 41.49992760967135 17.298392934703923 34 18.805668016194332 23.13475997686524 43.76229034123771 14.297281665702718 35 19.810966268751624 22.90950082377085 42.12793016735555 15.151602740121975 36 18.69899878465189 22.83118236009028 41.80508131257596 16.664737542681866 37 17.53679453007301 23.299339436782944 42.68455209178352 16.47931394136053 38 20.54517043810406 24.223045315122402 42.17547311765727 13.056311129116269 39 17.451708335750332 19.914148152445037 37.800916526480655 24.833226985323975 40 10.300441655044166 17.24779172477917 29.311947931194794 43.13981868898187 41 13.538327018361992 17.47886913436316 32.20052462838823 36.78227921888662 42 15.385728759588943 26.68403531625416 43.053987552467795 14.876248371689101 43 9.527260079417987 14.941595895771137 33.33526564447407 42.19587838033681 44 11.094983743613563 16.616349280074317 29.84498374361356 42.44368323269856 45 14.052646803586924 24.266705235753545 42.918715649407005 18.76193231125253 46 15.140041044021157 25.100442234875857 44.48073532387201 15.278781397230972 47 16.690751445086704 22.289017341040463 39.702312138728324 21.31791907514451 48 16.416702788614362 24.950151712180322 43.84337523479266 14.789770264412658 49 11.824216598544801 16.89422268603067 30.30118561034293 40.9803751050816 50 14.757230956310257 25.87220243782391 43.79976258722024 15.570804018645589 51 18.829944600748323 26.507526756968414 40.859123473620095 13.803405168663167 52 15.732358402971217 28.325208913649025 39.80095171773444 16.14148096564531 53 14.825429911412193 29.98957790515894 40.400092640843035 14.784899542585839 54 14.769373227617338 24.888593089877887 36.74402453845709 23.598009144047687 55 15.561534455823642 25.695770410229706 43.64693629578198 15.095758838164672 56 15.393076299641079 25.897302304040753 40.039944425147624 18.669676971170546 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.0 9 4.0 10 4.5 11 5.0 12 13.0 13 40.5 14 60.0 15 79.5 16 99.0 17 135.5 18 172.0 19 217.5 20 263.0 21 302.5 22 448.0 23 554.0 24 668.0 25 782.0 26 987.5 27 1193.0 28 1372.0 29 1730.5 30 1910.0 31 1982.0 32 2054.0 33 1967.5 34 1881.0 35 1673.5 36 1466.0 37 1281.0 38 979.5 39 863.0 40 784.0 41 705.0 42 668.0 43 631.0 44 602.5 45 574.0 46 604.5 47 719.0 48 803.0 49 833.0 50 863.0 51 918.5 52 974.0 53 1029.5 54 1134.0 55 1183.0 56 1237.5 57 1292.0 58 1400.0 59 1508.0 60 1644.0 61 1780.0 62 1731.0 63 1634.0 64 1586.0 65 1546.0 66 1506.0 67 1311.0 68 1116.0 69 979.0 70 842.0 71 700.5 72 499.0 73 439.0 74 348.5 75 258.0 76 210.0 77 162.0 78 115.5 79 51.0 80 33.0 81 20.0 82 7.0 83 7.0 84 7.0 85 4.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 1.0 95 2.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.028886706337743373 10 0.0 11 0.0 12 0.0 13 0.0 14 0.023109365070194697 15 0.15887688485758852 16 0.04333005950661505 17 0.01733202380264602 18 0.01733202380264602 19 0.023109365070194697 20 0.04333005950661505 21 0.005777341267548674 22 0.0 23 0.0 24 0.0 25 0.0 26 0.19931827373042926 27 0.037552718239066384 28 0.21376162689930095 29 0.04910740077416373 30 0.037552718239066384 31 0.265757698307239 32 0.025998035703969035 33 0.23975966260326997 34 0.10976948408342481 35 0.060662083309261074 36 0.1733202380264602 37 0.29464440464498237 38 0.1733202380264602 39 0.40441388872840717 40 0.583511468022416 41 0.8897105552024958 42 0.21087295626552657 43 0.33797446415159743 44 0.4910740077416373 45 0.13865619042116817 46 0.060662083309261074 47 0.05199607140793807 48 0.037552718239066384 49 0.34952914668669477 50 0.2282049800681726 51 0.4073025593621815 52 0.4448552776012479 53 0.21953896816684962 54 0.1733202380264602 55 0.15021087295626553 56 0.20220694436420358 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 34618.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.08897105552025 #Duplication Level Percentage of deduplicated Percentage of total 1 94.86645076474171 85.46420937084753 2 3.5271106550806426 6.355075394303541 3 0.7791708083496328 2.1058408920214915 4 0.2982011735659089 1.0745854757640534 5 0.17314906852214063 0.7799410711190711 6 0.09298746272485331 0.5026286902767346 7 0.05130342771026389 0.32353111098272574 8 0.035271106550806425 0.25420301577214166 9 0.025651713855131944 0.20798428563175228 >10 0.13787796197133423 2.0942862094863943 >50 0.009619392695674479 0.5257380553469294 >100 0.003206464231891493 0.3119764284476284 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTACCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 108 0.3119764284476284 No Hit TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 69 0.19931827373042926 No Hit TCTACCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCGAACCTGCGTGCAATC 61 0.17620890866023456 No Hit TCTACCGGGCTCGTCTTAATCAGCAACATCTTTTGATACCCAAAAAAAAAAAAAAA 52 0.15021087295626553 No Hit TCTACCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 43 0.12421283725229648 No Hit TCTACCGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGACAACCTGCGTGCAATC 41 0.11843549598474783 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.002888670633774337 0.0 0.0 0.0 0.0 11 0.005777341267548674 0.0 0.0 0.0 0.0 12 0.005777341267548674 0.0 0.0 0.0 0.0 13 0.005777341267548674 0.0 0.0 0.0 0.0 14 0.005777341267548674 0.0 0.0 0.0 0.0 15 0.011554682535097349 0.0 0.0 0.0 0.0 16 0.02022069443642036 0.0 0.0 0.0 0.0 17 0.023109365070194697 0.0 0.0 0.0 0.0 18 0.04333005950661505 0.0 0.0 0.0 0.0 19 0.054884742041712405 0.0 0.0 0.0 0.0 20 0.06643942457680975 0.0 0.0 0.0 0.0 21 0.06643942457680975 0.0 0.0 0.0 0.0 22 0.06643942457680975 0.0 0.0 0.0 0.0 23 0.07221676584435843 0.0 0.0 0.0 0.0 24 0.08377144837945577 0.0 0.0 0.0 0.0 25 0.0866601190132301 0.0 0.0 0.0 0.0 26 0.08954878964700445 0.0 0.0 0.0 0.0 27 0.09821480154832746 0.0 0.0 0.0 0.0 28 0.10976948408342481 0.0 0.0 0.0 0.0 29 0.11265815471719914 0.0 0.0 0.0 0.0 30 0.11265815471719914 0.0 0.0 0.0 0.0 31 0.11265815471719914 0.0 0.0 0.0 0.0 32 0.11265815471719914 0.0 0.0 0.0 0.0 33 0.11265815471719914 0.0 0.0 0.0 0.0 34 0.11265815471719914 0.0 0.0 0.0 0.0 35 0.11265815471719914 0.0 0.0 0.0 0.0 36 0.11265815471719914 0.0 0.0 0.0 0.0 37 0.11265815471719914 0.0 0.0 0.0 0.0 38 0.11265815471719914 0.0 0.0 0.0 0.0 39 0.11265815471719914 0.0 0.0 0.0 0.0 40 0.11265815471719914 0.0 0.0 0.0 0.0 41 0.11265815471719914 0.0 0.0 0.0 0.0 42 0.11265815471719914 0.0 0.0 0.0 0.0 43 0.11265815471719914 0.0 0.0 0.0 0.0 44 0.12132416661852216 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGCAC 20 3.8217002E-4 50.782547 39 ACAAACC 15 0.007863038 50.782543 38 GCGAACC 15 0.007863038 50.782543 39 ACCAACC 15 0.007863038 50.782543 38 GGCGAAC 15 0.007863038 50.782543 38 CCACCAG 15 0.007863038 50.782543 40 CGAACCT 15 0.007863038 50.782543 40 TCCGACC 15 0.007863038 50.782543 38 GCCCTTG 15 0.007863038 50.782543 38 AGAGCCC 20 3.877988E-4 50.632748 35 TACCCAA 20 3.877988E-4 50.632748 37 CGGCGAA 15 0.007955604 50.632744 37 CCGGCGA 15 0.007955604 50.632744 36 TAGAGCC 30 9.524938E-7 50.48382 34 TGTGAAA 15 0.0082859825 50.11533 49 ACAAAAT 15 0.0082859825 50.11533 48 CTGCAAT 15 0.0082859825 50.11533 49 GCCTGCA 15 0.0082859825 50.11533 47 ATTCAAG 15 0.0082859825 50.11533 33 CGCGCAA 15 0.0082859825 50.11533 47 >>END_MODULE