FastQCFastQC Report
Fri 17 Jun 2016
SRR1526365_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526365_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61931
Sequences flagged as poor quality0
Sequence length56
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC1340.21636983094088583No Hit
GTAATGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC1070.17277292470652825No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA1040.16792882401382184No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC800.12917601847217064No Hit
GTAATGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACCAAAAAAAAAAAAAAA760.12271721754856212No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC620.10011141431593225No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAAAA150.00809737750.4558139
ACGCACC150.00809737750.4558138
TTATTAT251.9562518E-550.4558138
GGCGAAC150.00809737750.4558138
ATTATTT251.9562518E-550.4558140
CGAACCT150.00809737750.4558140
AACTAAA150.00809737750.4558138
AGCACCA150.00809737750.4558138
TCACCGA203.9707872E-450.4558140
GTGGACC150.00809737750.4558138
CTATTGT150.00809737750.4558141
CGGCGAA150.00817633150.33224537
TTTATTA251.9848914E-550.33224537
CATCTCT150.00825585750.20928644
CGCAACA150.00825585750.20928644
GCGATGC150.00828249450.1684343
CGTTTTC150.00828249450.1684343
CCGACTC402.5356712E-950.16842743
GTTCGGT204.084475E-450.16842735
TATGAAG150.00830919450.12764448