##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526365_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	61931
Sequences flagged as poor quality	0
Sequence length	56
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.146905427007475	38.0	37.0	39.0	33.0	40.0
2	36.06206907687588	38.0	35.0	39.0	28.0	40.0
3	35.7993573493081	38.0	36.0	39.0	28.0	40.0
4	35.07889425328188	38.0	35.0	39.0	19.0	40.0
5	36.278761847862945	38.0	36.0	39.0	29.0	40.0
6	36.564789846764945	38.0	36.0	39.0	31.0	40.0
7	36.68028935428138	38.0	36.0	39.0	31.0	40.0
8	36.46873133002858	38.0	36.0	39.0	31.0	40.0
9	36.44544735269897	38.0	36.0	39.0	31.0	40.0
10	35.92777445867175	38.0	35.0	39.0	29.0	40.0
11	34.70266909948168	38.0	33.0	39.0	27.0	40.0
12	32.71439182315803	36.0	30.0	39.0	18.0	40.0
13	32.38073016744441	36.0	30.0	39.0	16.0	40.0
14	31.941515557636723	36.0	29.0	39.0	15.0	40.0
15	32.51121409310362	36.0	30.0	39.0	17.0	40.0
16	32.08151006765594	36.0	29.0	39.0	17.0	40.0
17	32.1795223716717	36.0	29.0	38.0	17.0	40.0
18	32.271947812888534	36.0	29.0	38.0	18.0	40.0
19	33.19663819411926	36.0	30.0	38.0	23.0	40.0
20	33.27632365051428	36.0	30.0	38.0	23.0	40.0
21	34.88776218694999	38.0	34.0	39.0	26.0	40.0
22	34.588768145193846	38.0	33.0	39.0	25.0	40.0
23	34.778673039350245	38.0	33.0	39.0	26.0	40.0
24	34.40885824546673	38.0	33.0	39.0	24.0	40.0
25	34.69753435274741	38.0	33.0	39.0	26.0	40.0
26	35.15815988761686	38.0	35.0	39.0	27.0	40.0
27	35.167315237926076	38.0	35.0	39.0	28.0	40.0
28	35.12128013434306	38.0	35.0	39.0	28.0	40.0
29	34.901939254977314	38.0	35.0	39.0	27.0	40.0
30	34.76281668308278	38.0	35.0	39.0	27.0	40.0
31	34.4927580694644	38.0	34.0	39.0	26.0	40.0
32	33.43751917456524	38.0	33.0	39.0	18.0	40.0
33	34.60740178585846	38.0	35.0	39.0	26.0	40.0
34	34.27763155770131	38.0	34.0	39.0	25.0	40.0
35	34.27343333710097	38.0	34.0	39.0	26.0	40.0
36	31.749156319129355	35.0	31.0	38.0	19.0	39.0
37	31.516203516817104	35.0	30.0	38.0	17.0	39.0
38	30.818087871986567	35.0	30.0	38.0	10.0	39.0
39	28.81652161276259	33.0	26.0	38.0	4.0	39.0
40	24.460706269880998	30.0	10.0	37.0	4.0	39.0
41	20.878493807624615	24.0	8.0	32.0	4.0	36.0
42	24.848282766304436	30.0	19.0	33.0	4.0	36.0
43	20.763430269170527	24.0	6.0	32.0	4.0	36.0
44	21.84870258836447	26.0	7.0	32.0	4.0	36.0
45	24.492047601362806	29.0	17.0	33.0	4.0	36.0
46	24.659378986291195	30.0	9.0	35.0	4.0	38.0
47	23.950945406985195	29.0	4.0	35.0	4.0	38.0
48	23.94640809933636	29.0	4.0	35.0	4.0	38.0
49	21.22502462417852	25.0	4.0	35.0	4.0	38.0
50	23.708853401366035	29.0	4.0	35.0	4.0	38.0
51	25.32389271931666	32.0	4.0	38.0	4.0	39.0
52	25.11435307035249	32.0	4.0	38.0	4.0	39.0
53	24.732524906751063	31.0	4.0	38.0	4.0	39.0
54	23.847588445205147	31.0	4.0	37.0	4.0	39.0
55	23.991958792850106	31.0	4.0	37.0	4.0	39.0
56	23.221666047698246	30.0	4.0	37.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	1.2996400559510093
1	2	2.2012529193923385
1	3	2.530159179864029
1	4	4.008587125874328
1	5	2.0668216460663515
1	6	1.8241213185448473
1	7	2.049901474079853
1	8	2.3259643466499043
1	9	2.2069523535304825
1	10	2.209582414118067
1	11	-6.817837198298996
1	12	-9.302125156639015
1	13	-21.293939136840585
1	14	-19.51949718464129
1	15	-12.785118999603263
1	16	-21.634048714938512
1	17	-21.83088511526213
1	18	-20.97464582563881
1	19	-7.440425571952801
1	20	-12.100467271593395
1	21	-8.86222233460477
1	22	-3.27098346346229
1	23	-3.9143630564625163
1	24	-3.8387717937585
1	25	-4.231846648131469
1	26	2.508731046357134
1	27	1.9114804826951257
1	28	2.1415097236619687
1	29	1.9987219917063257
1	30	2.2494140075935434
1	31	0.5906719835328929
1	32	-12.034369676996874
1	33	0.2360921975257284
1	34	0.2846872931372033
1	35	0.6614949969640591
1	36	4.269189096283522
1	37	3.878804546615811
1	38	4.831530736340049
1	39	7.20754861761214
1	40	8.949673168071612
1	41	-3.6238388448191117
1	42	-9.290825869204633
1	43	-11.983494798190002
1	44	-14.906869796989579
1	45	-15.326899231744047
1	46	-17.79722431945258
1	47	-17.24734335548504
1	48	-16.748585743033953
1	49	-14.049065515201889
1	50	-16.339822781378135
1	51	-17.78118816742927
1	52	-17.528074868926883
1	53	-17.7989116412582
1	54	-17.515445527517702
1	55	-17.28750829132965
1	56	-16.473436014876924
2	1	1.5590108725507363
2	2	2.1276250746801537
2	3	2.9058285239068695
2	4	4.188138665365621
2	5	2.342591391715615
2	6	1.9044426036854105
2	7	2.2026792518576315
2	8	2.416660464454452
2	9	2.350359314708527
2	10	2.1525208344661237
2	11	-5.038553396424831
2	12	-12.802627164671144
2	13	-13.530384919973116
2	14	-16.330240156528838
2	15	-10.767381382468056
2	16	-6.5108894110563185
2	17	-4.857993548997072
2	18	-12.869056802881111
2	19	-4.564254219877835
2	20	-6.44986486195484
2	21	1.9627441981930858
2	22	2.1841704856675648
2	23	1.7703758993607792
2	24	1.936495943858631
2	25	1.3340837400880758
2	26	1.3537779004401287
2	27	1.6141243916643333
2	28	2.0029555067944997
2	29	2.210067373232434
2	30	2.0455987465493664
2	31	-1.4091606804564023
2	32	-8.492870346340919
2	33	-1.8557752723537888
2	34	-1.2477758929404388
2	35	-1.600720531817732
2	36	3.4358557629501867
2	37	3.5491526055180884
2	38	4.664027389619839
2	39	6.805440183877202
2	40	9.121861923091693
2	41	2.808724742864957
2	42	-0.2115086001283295
2	43	-5.425261866463092
2	44	-11.672599381996273
2	45	-5.270172324113524
2	46	-13.568810664834107
2	47	-12.640080972620247
2	48	-11.90169819283315
2	49	-12.611983855228662
2	50	-11.698591188339314
2	51	-12.352975316023649
2	52	-12.307358670801047
2	53	-11.655839352101575
2	54	-15.770298271828278
2	55	-11.619000928545178
2	56	-11.223770686898343
3	1	1.4286279547408824
3	2	1.823915185618958
3	3	2.7274914130873498
3	4	4.044753242485989
3	5	1.649879951896935
3	6	1.7614150479177866
3	7	1.8363426181942657
3	8	1.870793829598199
3	9	2.223316489944118
3	10	2.0265516110377604
3	11	0.23593067848868543
3	12	0.4326108169583449
3	13	-6.951117354662369
3	14	-8.440701805103334
3	15	-3.775493037007003
3	16	1.130527742707251
3	17	1.8943624094903448
3	18	1.1542045593996981
3	19	0.23999246985137646
3	20	-0.0014573706032976474
3	21	2.206809568585548
3	22	3.132756910575111
3	23	2.5097293527784075
3	24	2.914666050025879
3	25	2.495601096643007
3	26	1.1982249957520708
3	27	1.9369205266995237
3	28	2.017747347424347
3	29	2.343193938901045
3	30	1.5918232485176347
3	31	1.598235239858525
3	32	0.4997337333441578
3	33	1.8264387321791915
3	34	2.5847422986377566
3	35	1.7306644139057568
3	36	4.307142891663059
3	37	4.315823248485998
3	38	5.1220972929957185
3	39	7.706310993849762
3	40	10.860976798434649
3	41	9.39252529159453
3	42	7.2545536687491605
3	43	1.7623797892687456
3	44	2.949015791569277
3	45	7.138034761655295
3	46	-2.077339831003737
3	47	-5.48345446659615
3	48	-1.8471831027699288
3	49	-9.848295438194187
3	50	-2.2881176108610823
3	51	-4.3773102796404935
3	52	-3.8392684882888197
3	53	-3.9857928293770115
3	54	-7.301611644129364
3	55	-4.775269567457261
3	56	-1.3870773790133377
4	1	1.9940845003954522
4	2	2.747549215688636
4	3	3.155159179864029
4	4	4.753957496244695
4	5	2.789043868288573
4	6	2.8148620592855877
4	7	2.4897162888946696
4	8	2.538927309612866
4	9	2.9060264276045515
4	10	2.1864342659699147
4	11	3.251607246145447
4	12	3.5358378063239506
4	13	4.831060863159415
4	14	3.1610583709142652
4	15	6.353769889285626
4	16	6.338173507283713
4	17	6.706151921774907
4	18	5.807761581768599
4	19	4.633648502121272
4	20	4.288421617295491
4	21	3.211851739469303
4	22	3.793831351352523
4	23	3.7708221287226706
4	24	3.6288207988340915
4	25	3.036671870387046
4	26	2.453175490801577
4	27	2.7957397419543852
4	28	1.9563245384767853
4	29	2.3552034731878067
4	30	2.9531177112972458
4	31	2.4934497613106714
4	32	3.6045192118920113
4	33	2.4722033086368427
4	34	2.9883909968409057
4	35	2.5272357377047996
4	36	4.602522429616851
4	37	4.601026768838032
4	38	5.650975180784499
4	39	8.008474543538064
4	40	10.86171020510865
4	41	4.8344944885142205
4	42	8.653618575239811
4	43	8.822060757365556
4	44	11.917204277084494
4	45	9.09439706455225
4	46	9.235183087954827
4	47	9.368397385255701
4	48	8.228266108817898
4	49	6.8583418922055195
4	50	8.687954996399643
4	51	7.019737758496653
4	52	7.4302584644064495
4	53	6.9464587291121696
4	54	2.4151100280378515
4	55	7.911565782744425
4	56	7.545082503641595
5	1	1.8185289448398976
5	2	2.4356973638367805
5	3	3.4329369576418074
5	4	4.832476014763216
5	5	2.2612660905107944
5	6	2.677454651878186
5	7	2.5226792518576318
5	8	3.1548532355387877
5	9	2.900841242419368
5	10	2.499026858562509
5	11	3.654940579478783
5	12	5.53954151002765
5	13	6.171060863159418
5	14	5.322169482025377
5	15	6.185992111507847
5	16	6.5070623961726
5	17	6.55911488473787
5	18	5.814798618805636
5	19	5.1006855391583095
5	20	5.169532728406608
5	21	3.3488887765063424
5	22	3.597905425426596
5	23	2.7123036102041524
5	24	3.547339317352609
5	25	2.7292644629796428
5	26	1.663175490801578
5	27	2.018702704917345
5	28	1.9015097236619667
5	29	2.6870553250396583
5	30	2.1460806742602045
5	31	3.0601164279773343
5	32	3.9978525452253493
5	33	2.3310921975257273
5	34	2.812465070914982
5	35	2.322050552519613
5	36	3.6858557629501867
5	37	4.833248991060252
5	38	5.5576418474511655
5	39	7.352548617612143
5	40	5.2680065014049475
5	41	2.490050044069779
5	42	8.385840797462034
5	43	12.643171868476667
5	44	11.785352425232642
5	45	8.794767434922619
5	46	10.614442347214084
5	47	10.486545533403849
5	48	10.389747590299379
5	49	10.509823373687002
5	50	9.501288329732976
5	51	9.25714516590406
5	52	8.429147353295338
5	53	9.1460883587418
5	54	9.290665583593409
5	55	9.533047264225907
5	56	9.617675096234187
6	1	2.2806342079977924
6	2	2.666749995415728
6	3	3.0776737997470676
6	4	4.883002330552685
6	5	3.0375818799844794
6	6	2.6521914939834446
6	7	2.792152936068156
6	8	3.264853235538787
6	9	2.9761044003141066
6	10	2.7579742269835634
6	11	4.387572158426153
6	12	6.613225720553967
6	13	5.725797705264675
6	14	6.872169482025381
6	15	7.065465795718371
6	16	7.256009764593649
6	17	7.124378042632603
6	18	6.2526933556477395
6	19	5.433843433895152
6	20	5.099532728406608
6	21	3.4936256186116026
6	22	4.249484372795017
6	23	3.515461504940994
6	24	4.036813001563139
6	25	2.9292644629796385
6	26	3.1979123329068386
6	27	3.3165974417594555
6	28	2.146772881556707
6	29	3.0833711145133478
6	30	3.050291200575998
6	31	3.304853270082596
6	32	4.993115703120083
6	33	3.2031974606836258
6	34	3.9387808603886683
6	35	2.5394189735722463
6	36	2.4884873418975566
6	37	2.478512148954991
6	38	3.209747110609058
6	39	4.819390722875298
6	40	0.5580065014049467
6	41	0.8716289914382003
6	42	5.588998692198874
6	43	10.504224500055614
6	44	9.420615583127379
6	45	6.980556908606832
6	46	10.365494978793034
6	47	11.169177112351221
6	48	9.912379169246748
6	49	12.22403390000279
6	50	10.52918306657508
6	51	10.313987271167218
6	52	9.455989458558495
6	53	11.005035727162849
6	54	10.565402425698672
6	55	10.329889369489063
6	56	10.716622464655238
7	1	1.907278944839895
7	2	2.510280697170117
7	3	3.0891869576418074
7	4	4.73080934809655
7	5	2.81334942384413
7	6	2.626204651878183
7	7	2.9943459185242958
7	8	2.9856865688721257
7	9	3.1062579090860325
7	10	2.81027685856251
7	11	3.8870239128121185
7	12	5.739541510027653
7	13	5.799810863159415
7	14	6.793002815358715
7	15	6.659325444841183
7	16	6.5291457295059345
7	17	6.252448218071205
7	18	6.303131952138973
7	19	4.368602205824974
7	20	5.264116061739941
7	21	2.8634721098396767
7	22	3.326238758759935
7	23	2.543970276870816
7	24	3.3244226506859462
7	25	2.7334311296463056
7	26	2.390675490801577
7	27	2.592036038250683
7	28	2.5685930569953044
7	29	2.884138658372997
7	30	2.332747340926872
7	31	1.983033094644
7	32	3.3232692118920113
7	33	2.694425530859064
7	34	2.041631737581646
7	35	2.4462172191862805
7	36	1.477522429616851
7	37	1.344082324393586
7	38	2.1718085141178314
7	39	4.947131950945472
7	40	-1.8524101652617198
7	41	-9.005783289263556
7	42	5.3550074641287
7	43	10.339421868476666
7	44	8.336185758565975
7	45	5.506434101589289
7	46	7.577775680547418
7	47	8.162378866737182
7	48	7.949330923632711
7	49	10.693990040353668
7	50	7.6358716630663075
7	51	8.979228499237394
7	52	8.600397353295339
7	53	9.1385883587418
7	54	9.158165583593409
7	55	8.601380597559238
7	56	8.148091762900854
8	1	1.864060859733513
8	2	2.252080342560184
8	3	3.0765539789184047
8	4	4.432050482848318
8	5	2.617649069234197
8	6	2.434050396559037
8	7	2.606934571006569
8	8	2.6882574908579357
8	9	2.662330604121493
8	10	2.704558773456128
8	11	3.888132068840484
8	12	5.845924488751056
8	13	6.820422565287075
8	14	6.399829056493463
8	15	6.799396366826993
8	16	6.455785800427918
8	17	6.312731906014466
8	18	5.956500746465213
8	19	4.840898305115754
8	20	5.048468898619369
8	21	2.583995159485063
8	22	3.5434373403202173
8	23	3.114005737863728
8	24	3.6154244237355897
8	25	2.9377751012775164
8	26	2.011686129099452
8	27	1.9395537687471318
8	28	1.5564033406832465
8	29	1.6910978782311474
8	30	1.3788466317070132
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115	10	1.187190123868632
115	11	-2.6038349307252986
115	12	-4.770662571605001
115	13	-2.3117962796977274
115	14	-8.156401946546048
115	15	-1.5566609497166404
115	16	-9.910692705868218
115	17	-10.621701441792744
115	18	-3.129691177112729
115	19	-3.5640083383927106
115	20	-4.1208754348587
115	21	0.2641948989553171
115	22	-0.035155799063197435
115	23	0.3747525897959889
115	24	0.7085638071485292
115	25	-0.46257227171423665
115	26	1.1725632459036177
115	27	1.0648251538969404
115	28	1.6415097236619687
115	29	1.834606345447824
115	30	2.064039857933679
115	31	0.4713409177732544
115	32	-5.536637250693019
115	33	1.014153422015525
115	34	0.9145058872415106
115	35	-0.08141883523548898
115	36	3.1705496405012106
115	37	2.0834530726929046
115	38	3.8533561331654482
115	39	5.7968343318978555
115	40	7.26004731773148
115	41	5.905356166518757
115	42	2.5340040627681546
115	43	0.4790902358236053
115	44	-1.6030149217061336
115	45	2.2402776390042547
115	46	-1.4188229589083612
115	47	-5.565087119657374
115	48	-4.111680981129194
115	49	-6.12956438141504
115	50	0.5672066970799143
115	51	0.6261247577407971
115	52	0.3803718430912575
115	53	-0.4596259269724854
115	54	0.20535946114442893
115	55	1.0461084887157028
115	56	3.7907363207239833
116	1	1.268717624085177
116	2	1.7589049110065957
116	3	2.484823750094641
116	4	3.1404005430650983
116	5	1.64334156220891
116	6	1.5914169160291252
116	7	1.9573962329897086
116	8	2.370513612897284
116	9	2.131218600909932
116	10	1.7224230849776063
116	11	-1.8348707412759353
116	12	-4.581213206953478
116	13	-6.150071212312284
116	14	-11.935377687785941
116	15	-4.169290907360075
116	16	-10.20218288684627
116	17	-12.335602096394208
116	18	-4.754257984967946
116	19	-5.565163517445463
116	20	-5.213674818763206
116	21	0.7724736821667193
116	22	1.0896035386341438
116	23	0.705133798883395
116	24	0.32658460037147563
116	25	0.46888710448907744
116	26	1.3258170002355385
116	27	1.739834780389046
116	28	1.1886795349827253
116	29	0.504225136360418
116	30	1.354759919543227
116	31	0.5233239751471501
116	32	-7.6895059453406915
116	33	0.8705261597898826
116	34	1.3785028067640397
116	35	1.2712958355384814
116	36	3.3981199138935843
116	37	3.573248991060254
116	38	4.1525475078285226
116	39	6.7806618251593065
116	40	9.082534803291743
116	41	6.07401230822072
116	42	1.616406835197882
116	43	-8.241356433410125
116	44	-2.2318173860881103
116	45	0.4458995103943195
116	46	-5.029142558446292
116	47	-9.653265787350866
116	48	-7.785535428568545
116	49	-7.886591720652623
116	50	-4.190975821210422
116	51	-2.011534079378958
116	52	-2.1148149108556034
116	53	-3.249383339371409
116	54	-6.491598567349987
116	55	-3.739028207472206
116	56	-1.0321362245205314
117	1	0.46794070954577904
117	2	1.0574620697191364
117	3	1.9182310752888654
117	4	2.919534838292627
117	5	1.0700896199225625
117	6	0.539807593054654
117	7	0.9967968989164575
117	8	0.788382647303493
117	9	-0.47033522816886375
117	10	0.837850387974278
117	11	-9.488588832285924
117	12	-12.40751731350176
117	13	-10.668939136840585
117	14	-11.610183459151092
117	15	-9.15930200613921
117	16	-12.11117289794505
117	17	-7.580885115262131
117	18	-3.875789616488479
117	19	-3.7999026961358062
117	20	-4.0122319774757464
117	21	-0.05993475290542705
117	22	-0.11738869222045878
117	23	0.16406831608650663
117	24	-4.6173665650003315
117	25	-0.8413237723144746
117	26	0.909057843742751
117	27	0.11164388138793413
117	28	0.5238626648384397
117	29	-0.8664740867250416
117	30	0.3131394977896207
117	31	-0.5898835720226643
117	32	-15.253323925362889
117	33	-1.1094960377683876
117	34	-1.0992996349673732
117	35	-0.40794944748038375
117	36	1.7887969394207737
117	37	1.8085431087073118
117	38	2.126465376862928
117	39	5.406078029376843
117	40	1.0991829719931836
117	41	2.816520632305071
117	42	-0.5212180260673804
117	43	-8.346239896229216
117	44	-7.184647574767357
117	45	-0.44699727095973074
117	46	-5.167322358668265
117	47	-11.454042701890268
117	48	-5.863781821465327
117	49	-9.483706038077706
117	50	-4.787535199678789
117	51	-4.116972481154761
117	52	-4.680852646704661
117	53	-5.320970464787614
117	54	-7.911687357583062
117	55	-6.048129206362329
117	56	-2.421148433177578
118	1	1.055550221435638
118	2	2.0712292787303994
118	3	2.629745468280106
118	4	3.61290154667811
118	5	2.192117154340579
118	6	1.731922736984565
118	7	1.7558707412193328
118	8	2.0605979163898525
118	9	1.3006284764619238
118	10	2.247111964945489
118	11	-7.048038143925474
118	12	-8.643437213376604
118	13	-6.722130626202286
118	14	-1.4086815818044087
118	15	-17.56230576083258
118	16	-7.927192922976339
118	17	-11.410672349304683
118	18	-4.224350317364575
118	19	-4.254846375735308
118	20	-4.845148122657225
118	21	0.8073994148042161
118	22	0.8519479786180852
118	23	0.5927291421190475
118	24	-3.2462777039239867
118	25	0.7250091438307038
118	26	1.5542393205888132
118	27	0.8650856836407499
118	28	1.3010841917470728
118	29	1.5315234101460433
118	30	1.9958679083027633
118	31	-0.18562825287372675
118	32	-9.97172192285976
118	33	0.23343262305764512
118	34	0.7273586879362597
118	35	-0.3441196602463421
118	36	2.5847919331629505
118	37	2.5945255868049344
118	38	4.057003549578823
118	39	5.657016702718522
118	40	8.392049054596434
118	41	6.98047557598467
118	42	2.849245052781182
118	43	-0.870019620885035
118	44	-0.9399667237035274
118	45	2.145618498752409
118	46	-5.085344886828466
118	47	-10.143028934681256
118	48	-8.944507728849556
118	49	-8.043155349717255
118	50	-5.952115925586174
118	51	-5.418599514947001
118	52	-5.297873923300404
118	53	-6.7909329178539455
118	54	-9.933589735555527
118	55	-8.096314437901754
118	56	-5.053814265467942
119	1	0.925708432019384
119	2	1.5944153125547302
119	3	2.2598600345648876
119	4	3.272476014763214
119	5	1.4599840392287433
119	6	0.9010443954679275
119	7	1.1770382262166024
119	8	1.2597250304105856
119	9	1.1631489347270616
119	10	0.5738986534343056
119	11	-8.76762352308532
119	12	-9.439945669459526
119	13	-1.8612468291482784
119	14	-3.573984364128467
119	15	-7.488110452594718
119	16	-12.394732475622273
119	17	-10.324474858851875
119	18	-2.3923808683738486
119	19	-3.3164939480211757
119	20	-3.6645698356959606
119	21	-1.2831625055449436
119	22	-1.0744022668810942
119	23	-1.2348758769753374
119	24	-7.275737605724313
119	25	-2.357915024199844
119	26	1.8057395933656792
119	27	0.5279334741481136
119	28	1.2056122877645308
119	29	2.0852604532447927
119	30	0.6532601614396967
119	31	-0.4103963925354819
119	32	-12.748044890672087
119	33	-0.1773693409358117
119	34	-1.0593298008798868
119	35	0.053589014058076145
119	36	2.9486762757706977
119	37	3.8553002731115384
119	38	4.824052103861419
119	39	5.697933232996753
119	40	7.440057783456229
119	41	5.51184491586465
119	42	1.2027638743851092
119	43	-3.9322127469079486
119	44	-2.248750138869923
119	45	-1.2531812830260982
119	46	-8.896583293811554
119	47	-11.611659594801278
119	48	-10.360765230213442
119	49	-9.89094585708223
119	50	-8.997942439497795
119	51	-10.181829193070298
119	52	-9.962903928755944
119	53	-12.327757795104354
119	54	-13.734462621534796
119	55	-11.914645043466402
119	56	-9.631555672996583
120	1	0.9917204342015964
120	2	1.7308037468155035
120	3	2.672298659769467
120	4	3.61290154667811
120	5	2.000627792638454
120	6	1.4127738008143567
120	7	1.5218281880278468
120	8	1.8265553631983664
120	9	1.768713582844903
120	10	1.587537496860385
120	11	-8.133144526904196
120	12	-2.4519478516744755
120	13	-5.317875307053352
120	14	-6.7278305179746205
120	15	-9.689965335300663
120	16	-11.544214199572082
120	17	-10.729821285474898
120	18	-5.203073721619894
120	19	-3.8080378650970097
120	20	-6.419616207763607
120	21	-0.6819622873234437
120	22	-0.06294563840318546
120	23	-0.5136538366043624
120	24	-5.778192597541008
120	25	-1.2111610689352545
120	26	1.1074308099505146
120	27	1.4608303644918124
120	28	1.3223607874917533
120	29	0.6379063888694461
120	30	1.6128891848985063
120	31	-5.845202720958834
120	32	-12.673849582434226
120	33	-4.681460993963633
120	34	-16.71944982270204
120	35	-5.578162213437832
120	36	-4.159888917900876
120	37	-4.043772285535489
120	38	-5.134485812123305
120	39	-2.4068130845155196
120	40	-4.352631796467392
120	41	-2.679098892100434
120	42	-7.491180479133712
120	43	-6.401934514502058
120	44	-9.854860340724803
120	45	-13.428849586353975
120	46	-15.468323610232723
120	47	-15.270688509149341
120	48	-13.49769921821126
120	49	-14.40485747737683
120	50	-13.228711670267025
120	51	-13.673918663883173
120	52	-13.446810093513172
120	53	-13.769656322109265
120	54	-14.274015267470421
120	55	-13.564399544284733
120	56	-11.245303627170069
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	529.0
5	44.0
6	43.0
7	60.0
8	142.0
9	178.0
10	114.0
11	122.0
12	119.0
13	134.0
14	200.0
15	257.0
16	385.0
17	495.0
18	625.0
19	706.0
20	969.0
21	1114.0
22	1338.0
23	1565.0
24	1806.0
25	1947.0
26	2027.0
27	2136.0
28	2355.0
29	2392.0
30	2566.0
31	3118.0
32	3578.0
33	4293.0
34	5039.0
35	6161.0
36	6544.0
37	5374.0
38	2650.0
39	806.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	100.0	0.0	0.0	0.0
2	0.0	0.0	100.0	0.0
3	0.0	100.0	0.0	0.0
4	0.0	100.0	0.0	0.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.36380810902456	0.3923721561092183	0.08719381246871519	0.1566259223975069
8	98.74376322035813	0.8913145274579775	0.16308472332111543	0.20183752886276662
9	94.18054366611213	3.530760906433221	1.3454363380873162	0.9432590893673382
10	41.307261306938365	39.02407518044275	9.31843503253621	10.350228480082672
11	29.748583054788547	30.89990150010496	17.663776259910545	21.68773918519595
12	35.17947393066477	17.143272351487944	19.97868595695209	27.698567760895187
13	24.331917779464245	14.75513070998369	24.26248566953545	36.65046584101662
14	22.89392079591705	18.255378254409198	26.208088377802184	32.64261257187157
15	26.13562831629352	17.856218454768992	24.648958198524653	31.359195030412838
16	27.523706443952634	22.46902411837875	23.919680790914818	26.08758864675379
17	29.535360713189814	23.485521406999467	21.12598717679549	25.853130703015232
18	29.80815812421277	26.097277395601203	20.343635952588574	23.750928527597456
19	29.28570275027858	27.65297718060109	19.085609082540657	23.975710986579674
20	26.14363248691607	29.92181947405828	20.011953220908445	23.922594818117208
21	24.68469923294308	24.13403310456197	27.720629794105772	23.46063786838918
22	28.066719413540874	23.947619124509533	24.746895738806092	23.238765723143498
23	26.292163859779432	27.66304435581534	25.670504270882113	20.374287513523115
24	27.72763236505143	25.76092748381263	23.548788167476708	22.96265198365923
25	25.314064266106897	27.689326659131275	23.734861940901016	23.261747133860812
26	22.72072742569612	23.181840244632486	31.066060478586564	23.031371851084828
27	25.066227305033273	20.273631840796018	29.51476384312205	25.14537701104865
28	24.10480349344978	23.04544719391881	30.043668122270745	22.806081190360665
29	23.75030283453121	23.598481789550192	31.879189211015103	20.772026164903494
30	21.766169154228855	23.633456096142663	35.28300058150805	19.317374168120438
31	20.415331326276263	24.32261823832184	37.00592405555016	18.256126379851736
32	23.273578811369507	20.802648578811368	36.4437984496124	19.479974160206716
33	21.79800365618883	22.534094769708638	39.19887402326371	16.46902755083882
34	18.238973380957766	23.03266368205841	43.37271507765907	15.355647859324744
35	17.981322303367158	26.99056420862147	41.310670199702706	13.717443288308667
36	18.409432772089865	25.84146085043751	40.031054393711486	15.71805198376114
37	19.014392332723535	25.979050980265185	39.443733911832794	15.562822775178486
38	21.03586559296271	26.22327867792115	39.32764140875133	13.4132143203648
39	18.39993515441355	20.747345383804817	35.81908081381211	25.033638647969525
40	12.22099483414016	16.230221904545306	29.646837129211473	41.901946132103056
41	13.401557865919239	18.880161653032623	31.246944562135386	36.47133591891275
42	16.463602090513405	24.942154911573873	42.77624063556785	15.81800236234487
43	10.847298304755114	14.68023726945642	32.45275679880717	42.019707626981294
44	11.716276124129196	16.376804533865965	30.18301099365064	41.7239083483542
45	17.842015067740164	23.544152358780355	41.13881074789019	17.47502182558929
46	16.017064184603452	25.24885269213367	41.24006205158038	17.494021071682504
47	14.809071525489436	22.200684887252052	39.671771015054595	23.318472572203916
48	17.279762193250296	25.128031147514502	42.12830578846185	15.463900870773356
49	12.386956662668958	16.860069365660756	29.076853262455028	41.67612070921526
50	15.28040786598689	25.04167678239055	42.49089584850692	17.187019503115643
51	17.121511684439113	26.5274041422228	40.3283311185298	16.022753054808284
52	14.989946163326199	26.945903872348705	40.80073944347149	17.263410520853604
53	15.867904989968288	27.33318231829655	41.26593747977477	15.53297521196039
54	14.086829978171235	23.82892715660118	36.028781631498106	26.05546123372949
55	16.373871687857903	26.75757869876088	40.1986476430813	16.669901970299914
56	16.83602472411896	26.614025436070033	38.54082392155594	18.009125918255073
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	3.5
7	7.0
8	32.0
9	57.0
10	132.0
11	207.0
12	241.5
13	274.0
14	272.0
15	337.5
16	403.0
17	440.5
18	478.0
19	557.5
20	637.0
21	770.5
22	1093.5
23	1283.0
24	1598.5
25	1914.0
26	2182.0
27	2450.0
28	2664.0
29	3023.0
30	3168.0
31	3040.0
32	2912.0
33	2761.0
34	2610.0
35	2336.0
36	2062.0
37	1769.0
38	1380.5
39	1285.0
40	1246.0
41	1207.0
42	1172.0
43	1137.0
44	1118.0
45	1099.0
46	1192.5
47	1399.0
48	1512.0
49	1617.5
50	1723.0
51	1885.0
52	2047.0
53	2169.5
54	2482.5
55	2673.0
56	2868.5
57	3064.0
58	3237.0
59	3410.0
60	3421.5
61	3433.0
62	3296.5
63	2902.5
64	2645.0
65	2255.5
66	1866.0
67	1642.0
68	1418.0
69	1200.0
70	982.0
71	827.0
72	570.0
73	468.0
74	372.0
75	276.0
76	215.5
77	155.0
78	121.0
79	57.5
80	28.0
81	17.0
82	6.0
83	5.0
84	4.0
85	3.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.029064604156238395
10	0.0
11	0.003229400461804266
12	0.0
13	0.0
14	0.024220503463531995
15	0.1856905265537453
16	0.04521160646525973
17	0.019376402770825595
18	0.008073501154510665
19	0.01614700230902133
20	0.037138105310749056
21	0.009688201385412798
22	0.0
23	0.0
24	0.0
25	0.001614700230902133
26	0.2002228286318645
27	0.037138105310749056
28	0.16308472332111543
29	0.02583520369443413
30	0.037138105310749056
31	0.24059033440441782
32	0.017761702539923464
33	0.1905346272464517
34	0.09365261339232371
35	0.06297330900518319
36	0.16792882401382184
37	0.26158143740614553
38	0.14370832055028984
39	0.39721625680192474
40	0.6022831861264957
41	0.9123056304597051
42	0.20668162955547303
43	0.3697663528765885
44	0.5651450808157465
45	0.12433191777946424
46	0.07589091085240025
47	0.037138105310749056
48	0.05167040738886826
49	0.3697663528765885
50	0.2357462337117114
51	0.3633075519529799
52	0.42466616072726093
53	0.20506692932457088
54	0.13886421985758343
55	0.18246112609194104
56	0.20829632978637516
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	61931.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.19865656940789
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.95033609243931	81.92343091505062
2	3.984963798307502	6.94966979380278
3	0.8684702701701756	2.2718832248793013
4	0.41664351980445535	1.4532302078119197
5	0.209247634390682	0.9123056304597051
6	0.1518434161065126	0.7944325136038495
7	0.08518045293779976	0.5199334743504869
8	0.05555246930726071	0.3875280554165119
9	0.04259022646889988	0.3342429477967415
>10	0.22220987722904284	3.4489996932069564
>50	0.007406995907634761	0.4472719639598909
>100	0.005555246930726071	0.5570715796612359
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC	134	0.21636983094088583	No Hit
GTAATGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC	107	0.17277292470652825	No Hit
GTAATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA	104	0.16792882401382184	No Hit
GTAATGGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC	80	0.12917601847217064	No Hit
GTAATGGGGACACGCAGGGATGAGATGGCCAAAAAAAAACCAAAAAAAAAAAAAAA	76	0.12271721754856212	No Hit
GTAATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	62	0.10011141431593225	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.001614700230902133	0.0	0.0	0.0	0.0
11	0.001614700230902133	0.0	0.0	0.0	0.0
12	0.003229400461804266	0.0	0.0	0.0	0.0
13	0.003229400461804266	0.0	0.0	0.0	0.0
14	0.004844100692706399	0.0	0.0	0.0	0.0
15	0.011302901616314932	0.0	0.0	0.0	0.0
16	0.01614700230902133	0.0	0.0	0.0	0.0
17	0.017761702539923464	0.0	0.0	0.0	0.0
18	0.019376402770825595	0.0	0.0	0.0	0.0
19	0.024220503463531995	0.0	0.0	0.0	0.0
20	0.03067930438714053	0.0	0.0	0.0	0.0
21	0.03067930438714053	0.0	0.0	0.0	0.0
22	0.033908704848944794	0.0	0.0	0.0	0.0
23	0.046826306696161855	0.0	0.0	0.0	0.0
24	0.05489980785067252	0.0	0.0	0.0	0.0
25	0.06297330900518319	0.0	0.0	0.0	0.0
26	0.06781740969788959	0.0	0.0	0.0	0.0
27	0.06943210992879172	0.0	0.0	0.0	0.0
28	0.07104681015969386	0.0	0.0	0.0	0.0
29	0.07104681015969386	0.0	0.0	0.0	0.0
30	0.07104681015969386	0.0	0.0	0.0	0.0
31	0.07427621062149811	0.0	0.0	0.0	0.0
32	0.07427621062149811	0.0	0.0	0.0	0.0
33	0.07427621062149811	0.0	0.0	0.0	0.0
34	0.07427621062149811	0.0	0.0	0.0	0.0
35	0.07427621062149811	0.0	0.0	0.0	0.0
36	0.07427621062149811	0.0	0.0	0.0	0.0
37	0.07427621062149811	0.0	0.001614700230902133	0.0	0.0
38	0.07427621062149811	0.0	0.001614700230902133	0.0	0.0
39	0.07427621062149811	0.0	0.001614700230902133	0.0	0.0
40	0.07427621062149811	0.0	0.001614700230902133	0.0	0.0
41	0.07589091085240025	0.0	0.001614700230902133	0.0	0.0
42	0.07750561108330238	0.0	0.001614700230902133	0.0	0.0
43	0.08073501154510665	0.0	0.001614700230902133	0.0	0.0
44	0.08396441200691092	0.0	0.001614700230902133	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTAAAA	15	0.008097377	50.45581	39
ACGCACC	15	0.008097377	50.45581	38
TTATTAT	25	1.9562518E-5	50.45581	38
GGCGAAC	15	0.008097377	50.45581	38
ATTATTT	25	1.9562518E-5	50.45581	40
CGAACCT	15	0.008097377	50.45581	40
AACTAAA	15	0.008097377	50.45581	38
AGCACCA	15	0.008097377	50.45581	38
TCACCGA	20	3.9707872E-4	50.45581	40
GTGGACC	15	0.008097377	50.45581	38
CTATTGT	15	0.008097377	50.45581	41
CGGCGAA	15	0.008176331	50.332245	37
TTTATTA	25	1.9848914E-5	50.332245	37
CATCTCT	15	0.008255857	50.209286	44
CGCAACA	15	0.008255857	50.209286	44
GCGATGC	15	0.008282494	50.16843	43
CGTTTTC	15	0.008282494	50.16843	43
CCGACTC	40	2.5356712E-9	50.168427	43
GTTCGGT	20	4.084475E-4	50.168427	35
TATGAAG	15	0.008309194	50.127644	48
>>END_MODULE