FastQCFastQC Report
Fri 17 Jun 2016
SRR1526364_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526364_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24682
Sequences flagged as poor quality0
Sequence length56
%GC54

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC470.19042217000243092No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC470.19042217000243092No Hit
CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC440.17826756340653108No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC430.17421602787456447No Hit
CCTAGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT380.1539583502147314No Hit
CCTAGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCCGTCGACCGTGCGCTT280.11344299489506524No Hit
CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC260.10533992383113198No Hit
CCTAGAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC250.10128838829916538No Hit
CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACCGCTCTTC250.10128838829916538No Hit
CCTAGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG250.10128838829916538No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCTTT251.921807E-550.453838
GACCGCT150.00817225350.24744446
TCTTGTC150.00817225350.24744442
CCTCATG150.00817225350.24744448
CTCATGG150.00817225350.24744449
TGCGGAG150.00817225350.24744444
CCAACAC204.0454313E-450.14489743
ATGTCGT150.00830557150.0427750
TTCGTGG204.127894E-449.9410615
GGCTCTC204.127894E-449.941069
CGCGCAG204.127894E-449.9410632
GCTGGCG150.00837283349.94105513
ATTCGTG150.00837283349.94105514
ATTCAAG150.00837283349.94105533
CGCCGGT150.00837283349.94105532
AGGGGAC950.049.839766
AGGGTGG355.140282E-849.839766
AGGGGTC451.2914825E-1049.8397566
AGGGGTA150.008440549.8397566
GCCTCGC150.008440549.83975628
Produced by FastQC (version 0.11.5)