##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526364_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24682 Sequences flagged as poor quality 0 Sequence length 56 %GC 54 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.64083137509116 39.0 38.0 40.0 34.0 40.0 2 37.2744105015801 39.0 38.0 40.0 33.0 40.0 3 36.953447856737704 39.0 38.0 40.0 32.0 40.0 4 35.61858844502066 39.0 37.0 40.0 22.0 40.0 5 36.412770439996756 39.0 36.0 39.0 30.0 40.0 6 34.79693703913783 38.0 35.0 39.0 19.0 40.0 7 36.596791183858684 38.0 37.0 39.0 31.0 40.0 8 36.46681792399319 38.0 36.0 39.0 31.0 40.0 9 36.623450287659026 38.0 37.0 39.0 31.0 40.0 10 35.34442103557248 38.0 35.0 39.0 27.0 40.0 11 33.584069362288304 36.0 31.0 39.0 24.0 40.0 12 32.00858925532777 35.0 28.0 39.0 16.0 40.0 13 31.801515274288956 36.0 28.0 39.0 15.0 40.0 14 31.42221051778624 36.0 27.0 39.0 14.0 40.0 15 32.02459282067904 36.0 28.0 39.0 16.0 40.0 16 32.45924155254841 36.0 30.0 39.0 17.0 40.0 17 32.534843205574916 36.0 30.0 39.0 18.0 40.0 18 32.69285309132161 36.0 30.0 39.0 20.0 40.0 19 33.611984442103555 36.0 31.0 39.0 24.0 40.0 20 33.67150149906815 36.0 31.0 39.0 24.0 40.0 21 35.270439996758775 38.0 35.0 39.0 27.0 40.0 22 34.88866380358156 38.0 34.0 39.0 26.0 40.0 23 35.058301596305 38.0 34.0 39.0 27.0 40.0 24 34.698363179645085 38.0 33.0 39.0 25.0 40.0 25 35.0467547200389 38.0 34.0 39.0 27.0 40.0 26 35.30670123976987 38.0 35.0 39.0 28.0 40.0 27 35.28077141236528 38.0 35.0 39.0 28.0 40.0 28 35.17883477838101 38.0 35.0 39.0 28.0 40.0 29 35.016692326391706 38.0 35.0 39.0 27.0 40.0 30 34.884531237338955 38.0 35.0 39.0 27.0 40.0 31 34.43788996029495 38.0 34.0 39.0 25.0 40.0 32 33.488615185155176 38.0 33.0 39.0 19.0 40.0 33 34.65456608054453 38.0 34.0 39.0 26.0 40.0 34 34.31573616400616 38.0 34.0 39.0 25.0 40.0 35 34.45421764848878 38.0 34.0 39.0 26.0 40.0 36 32.57807308970099 35.0 31.0 38.0 23.0 39.0 37 32.34608216514059 35.0 31.0 38.0 23.0 39.0 38 31.672028198687304 35.0 30.0 38.0 18.0 39.0 39 29.488129000891337 34.0 27.0 38.0 7.0 39.0 40 25.556235313183695 31.0 13.0 37.0 4.0 39.0 41 22.403451908273237 26.0 10.0 33.0 4.0 37.0 42 25.947451584150393 30.0 22.0 34.0 4.0 37.0 43 22.008953893525646 26.0 8.0 33.0 4.0 37.0 44 22.930556680982093 28.0 10.0 33.0 4.0 37.0 45 25.53411392917916 30.0 21.0 34.0 4.0 37.0 46 25.397374604975287 30.0 17.0 36.0 4.0 38.0 47 24.816019771493394 30.0 12.0 36.0 4.0 38.0 48 24.789684790535613 30.0 13.0 35.0 4.0 38.0 49 22.09577829997569 26.0 5.0 35.0 4.0 38.0 50 24.66505955757232 30.0 11.0 35.0 4.0 38.0 51 26.24013451097966 32.0 8.0 38.0 4.0 40.0 52 25.98047159873592 32.0 4.0 38.0 4.0 40.0 53 25.621100397050483 32.0 4.0 38.0 4.0 39.0 54 24.793574264646303 31.0 4.0 38.0 4.0 39.0 55 25.073211247062638 31.0 4.0 38.0 4.0 39.0 56 24.464143910542095 31.0 4.0 38.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 0.9429694354046987 1 2 1.3466019153027133 1 3 1.0494120545742405 1 4 3.5085476011335004 1 5 2.2580546382815854 1 6 4.334125727597318 1 7 2.027873956451863 1 8 2.04029922800661 1 9 1.9948120086808458 1 10 3.0140109512049733 1 11 -2.3922099059636572 1 12 -6.736578534868119 1 13 -19.962227377738856 1 14 -17.35481795163404 1 15 -10.8154032184985 1 16 -20.47753860987999 1 17 -21.80278483880269 1 18 -19.8047114663365 1 19 -5.2049578559499246 1 20 -11.183488837345315 1 21 -8.646121467550856 1 22 -3.1119818037524745 1 23 -2.919329688586423 1 24 -3.674934388514515 1 25 -3.047013830160367 1 26 1.8588118282946837 1 27 2.3027406867874944 1 28 2.281767717891782 1 29 2.011115354256738 1 30 1.9431500606527692 1 31 0.8615470245754793 1 32 -12.20306477686615 1 33 0.3633645485828296 1 34 1.536150952319808 1 35 0.89783549783062 1 36 2.299929888834921 1 37 3.8486350915542182 1 38 4.618879422380431 1 39 5.810392850434464 1 40 7.7517227839986695 1 41 -6.7356987027923 1 42 -13.421730230925077 1 43 -14.117160914549899 1 44 -16.060091426572182 1 45 -16.34059609171871 1 46 -18.442009601617457 1 47 -18.08315268976247 1 48 -15.959164554166602 1 49 -15.375868350092242 1 50 -15.99449140996202 1 51 -17.39986669494658 1 52 -17.181922452780235 1 53 -17.67063234940874 1 54 -17.883949953786484 1 55 -17.295194326851295 1 56 -16.56147937785841 2 1 1.3563027687380327 2 2 1.7987231274239264 2 3 2.322139327301514 2 4 3.718244570830471 2 5 2.7501758504027976 2 6 4.71473178820338 2 7 2.1975709261488348 2 8 2.2390871067944857 2 9 2.2154180692869048 2 10 2.874617011811033 2 11 -3.394634148387901 2 12 -9.297790656080242 2 13 -11.163439498950979 2 14 -13.231181587997675 2 15 -6.194797157892438 2 16 -3.0932961856375627 2 17 -1.7118757478936004 2 18 -9.771984193609224 2 19 -3.0243517953438612 2 20 -3.8089433827998604 2 21 2.026605805176416 2 22 2.4383212265505563 2 23 2.484306675049943 2 24 2.432944399364274 2 25 2.538440715294179 2 26 2.7388118282946863 2 27 2.076074020120828 2 28 2.5193434754675366 2 29 2.7492971724385598 2 30 2.112847030349741 2 31 -0.36875600572755474 2 32 -10.809125382926755 2 33 -0.5166354514171729 2 34 -1.022636926468067 2 35 -0.4924675324724106 2 36 4.092657161562197 2 37 4.01711994003907 2 38 5.372818816319821 2 39 7.7922110322526414 2 40 10.226874299150186 2 41 8.686119479025884 2 42 3.7322091630143177 2 43 -0.5789790963680836 2 44 -9.691606578087335 2 45 -0.08847487959749856 2 46 -11.760797480405337 2 47 -11.788607235217016 2 48 -9.230679705681755 2 49 -12.880110774334666 2 50 -9.186006561477171 2 51 -9.198654573734462 2 52 -8.838892149749933 2 53 -8.326389925166316 2 54 -15.49970752954406 2 55 -8.700042811699781 2 56 -8.76147937785841 3 1 1.510148922584186 3 2 2.1763455050463065 3 3 2.5622325673947515 3 4 4.056239908825809 3 5 3.1021572023841486 3 6 4.859253932725522 3 7 2.791976520554428 3 8 2.4885043562117417 3 9 2.334299188168025 3 10 3.608882746076766 3 11 2.206764453010699 3 12 3.3106009523113684 3 13 -3.8627401982516787 3 14 -3.543535900351987 3 15 -3.1271980902933727 3 16 3.251692159350778 3 17 2.7100356740178206 3 18 1.5963141746891445 3 19 1.9663241953321275 3 20 1.2749727011162264 3 21 3.420545199115807 3 22 4.049043837273167 3 23 3.7873369780802406 3 24 3.7989117653316384 3 25 2.35196052881399 3 26 3.123427212910073 3 27 2.742740686787492 3 28 3.300229256353319 3 29 1.572140995282382 3 30 1.9893039068066187 3 31 1.5077008707293231 3 32 1.4636018898005148 3 33 1.6884927537110315 3 34 2.5228176189864726 3 35 1.8198867798819052 3 36 4.025058093963128 3 37 4.117353040272167 3 38 4.97887942238043 3 39 7.738597978639593 3 40 9.429671501947386 3 41 10.017121810028215 3 42 8.18442361522877 3 43 3.37206985468087 3 44 4.511190624709869 3 45 6.007096215973597 3 46 0.45132373171587403 3 47 -5.42263986924965 3 48 1.818271343269295 3 49 -10.789201683425576 3 50 -0.23961961509022345 3 51 -0.2942256693055576 3 52 -0.5521788630366444 3 53 -2.391657990434382 3 54 -3.5649755948121253 3 55 -1.5228866345436032 3 56 1.9821103657313337 4 1 1.7058962646729867 4 2 2.4890409396929627 4 3 2.537216932623018 4 4 4.260742723084725 4 5 2.48927415047671 4 6 4.776076947109516 4 7 2.08153249303723 4 8 2.3602992280066104 4 9 1.8377388379491322 4 10 3.5457182682781436 4 11 4.140473020865606 4 12 5.652689757814805 4 13 5.884601890553828 4 14 3.7954259508049866 4 15 6.1592309278429624 4 16 7.067827243778549 4 17 5.880141990465599 4 18 5.491873899517163 4 19 5.427237266001299 4 20 3.712120918752248 4 21 3.802659020254019 4 22 2.944603562101186 4 23 4.023109335803824 4 24 3.35238268465622 4 25 3.383230072278657 4 26 3.69003134048981 4 27 1.7671309306899303 4 28 2.51786527886739 4 29 2.979895842061616 4 30 3.021198841140574 4 31 3.003010439209625 4 32 4.2359596133777515 4 33 3.190681621753562 4 34 4.197614366953957 4 35 2.440274522220868 4 36 5.819929888834924 4 37 4.92473265252983 4 38 5.539855032136529 4 39 7.659173338239338 4 40 9.101966686437695 4 41 7.28478910208575 4 42 9.191928305660287 4 43 7.676009817157418 4 44 11.947713451476595 4 45 10.114038054622753 4 46 8.459453813016687 4 47 8.342213163896066 4 48 6.38425007997974 4 49 3.080717015761415 4 50 7.136240297355055 4 51 6.777694280663173 4 52 6.004419010634397 4 53 4.322538382298578 4 54 1.8565378510915664 4 55 4.418952014612117 4 56 6.019008427019639 5 1 1.644591057026318 5 2 2.5055208342216346 5 3 2.5088715140337 5 4 4.072871925457825 5 5 2.8623789626059093 5 6 4.221693295164883 5 7 2.471117199695108 5 8 2.4954343631417473 5 9 2.614271468140302 5 10 3.354551491745511 5 11 4.5775198237660675 5 12 6.228826870537283 5 13 6.5793942438827635 5 14 6.134911778095688 5 15 6.609461646366363 5 16 7.185164092822713 5 17 5.791809755791906 5 18 5.71204529042026 5 19 4.5366637656716975 5 20 4.7905652167087425 5 21 2.672797451368062 5 22 2.1377479259772514 5 23 2.2287784195216815 5 24 3.4288493952692676 5 25 1.8308240076774709 5 26 3.1171902066730652 5 27 2.634632578679387 5 28 3.2752812314052946 5 29 2.525709948851336 5 30 2.3053122228149334 5 31 3.3912767543052027 5 32 3.7969352231338505 5 33 4.159040224258504 5 34 2.423718519887373 5 35 3.889186849181975 5 36 3.5226325915376258 5 37 4.276743199662324 5 38 4.735636179137188 5 39 7.069852309893921 5 40 6.765776838052723 5 41 1.8015985945049984 5 42 7.959891390696544 5 43 11.846082328693342 5 44 11.000449113968358 5 45 9.726430935308315 5 46 9.469341749733896 5 47 10.297387850778069 5 48 10.947862472860422 5 49 8.841428947205056 5 50 10.614157238686627 5 51 9.166619791539901 5 52 7.966185655327873 5 53 10.222340623564236 5 54 8.562536532700005 5 55 8.487508375851405 5 56 9.887169270790238 6 1 1.758263553051755 6 2 2.6454254447144834 6 3 2.9023532310448275 6 4 4.248547601133502 6 5 3.1262899323992315 6 6 5.212949257009086 6 7 2.75728572115775 6 8 2.9191227574183785 6 9 2.9195178910337845 6 10 4.255187421793202 6 11 5.377201858742225 6 12 7.529303818073053 6 13 8.281302034025845 6 14 7.699299695424788 6 15 7.2445967815014995 6 16 6.894226096002367 6 17 7.520744572962013 6 18 6.808229710134089 6 19 5.150336261697134 6 20 5.295334692066454 6 21 3.165643238331498 6 22 3.937429960953409 6 23 3.6500820761194603 6 24 2.998006787956072 6 25 3.6400449933690453 6 26 3.4741059459417443 6 27 3.242740686787492 6 28 3.026473600244721 6 29 3.6075859424920367 6 30 3.349032413593946 6 31 2.3674293775166504 6 32 4.4734058113691475 6 33 3.30689396034753 6 34 3.9149744817315764 6 35 3.8025413801835626 6 36 4.378753418246692 6 37 2.9627527386130446 6 38 4.067114716498075 6 39 5.9633340269050485 6 40 1.917605136939848 6 41 0.2984189442665226 6 42 7.279446239663159 6 43 12.367544967803042 6 44 11.048143867545466 6 45 7.846462731810703 6 46 10.588578633676658 6 47 11.403906133766942 6 48 12.126717798774571 6 49 12.955896355790113 6 50 10.436096825332097 6 51 10.150721540347533 6 52 9.82866578251388 6 53 11.719955885885376 6 54 12.693697105037046 6 55 11.667158614325174 6 56 10.532638269200415 7 1 2.3658265782618386 7 2 2.929459058159857 7 3 3.1351263402885294 7 4 4.691404743990645 7 5 3.5380546382815865 7 6 5.654125727597318 7 7 3.4278739564518688 7 8 3.3602992280066104 7 9 3.1262405801094104 7 10 4.7854395226335384 7 11 6.190647236893483 7 12 8.309135750846167 7 13 7.44348690797543 7 14 7.63375347693739 7 15 8.230311067215787 7 16 7.225318532977152 7 17 7.3686437326258805 7 18 6.869574247949213 7 19 5.60075642976436 7 20 5.079368305511828 7 21 4.296735675306287 7 22 3.608018196247528 7 23 3.9778131685564304 7 24 3.645065611485485 7 25 3.6215575984110586 7 26 2.8245261140089752 7 27 3.5427406867874893 7 28 2.790339146463211 7 29 3.416829639971027 7 30 3.286007203509918 7 31 4.432975596004049 7 32 4.682649508848137 7 33 3.483364548582827 7 34 3.5990080951769485 7 35 3.6806926406877665 7 36 0.848501317406356 7 37 1.5400636629827922 7 38 2.9217365652375733 7 39 4.1818214218630345 7 40 -0.4882772160013289 7 41 -9.809984417078015 7 42 5.338269769074923 7 43 9.802839085450099 7 44 8.145622859142104 7 45 5.242261051138435 7 46 7.203704684096827 7 47 7.128275881666099 7 48 7.23512116011911 7 49 10.201274507050616 7 50 7.79408001860941 7 51 10.19441901933913 7 52 9.772363261505475 7 53 10.620796222019834 7 54 10.504621474784948 7 55 10.67051995886299 7 56 10.267092050713021 8 1 2.022969435404697 8 2 2.4047837334845354 8 3 2.6857756909378736 8 4 3.68794154052744 8 5 2.5077516079785553 8 6 4.623822697294287 8 7 2.652116380694288 8 8 2.9663598340672195 8 9 2.306327160195991 8 10 3.510980648174666 8 11 5.120517366763615 8 12 7.399179040889457 8 13 7.654742319230838 8 14 7.950636593820509 8 15 7.956717993622711 8 16 5.785491693150316 8 17 6.894184858167005 8 18 6.076500654875627 8 19 3.6726179016258342 8 20 5.675905102048624 8 21 3.7235755021461117 8 22 4.044381832611158 8 23 4.181276372019639 8 24 3.9177928842127585 8 25 3.8717740486275076 8 26 3.4357815252643817 8 27 2.470013414060219 8 28 3.216313172437232 8 29 2.809903233044615 8 30 3.2946652121679207 8 31 4.540334903363352 8 32 5.736329162527788 8 33 3.240940306158585 8 34 4.159181255350113 8 35 3.9620779220730427 8 36 2.3653844342894708 8 37 2.3807563036754296 8 38 3.5849400284410393 8 39 4.701301941343555 8 40 -1.6216105493346618 8 41 -10.132062339155937 8 42 5.004936435741591 8 43 9.057384539995553 8 44 8.429605543124786 8 45 5.850919059796443 8 46 8.693747974140116 8 47 8.302301855692075 8 48 8.132956657954608 8 49 10.847161952938063 8 50 8.632175256704649 8 51 8.983163608083721 8 52 9.070198759340975 8 53 10.06754946877308 8 54 10.924534894698368 8 55 10.209048097391126 8 56 10.359732743353714 9 1 2.287675317757639 9 2 2.9689548564791863 9 3 3.2552944075154144 9 4 4.954429954074676 9 5 3.4792311088698185 9 6 5.830596315832615 9 7 3.374932779981279 9 8 3.4779462868301394 9 9 3.2136355380926105 9 10 4.8728344806167385 9 11 6.288966564624573 9 12 8.941068523955408 9 13 8.281302034025845 9 14 9.258123224836549 9 15 8.332832075619145 9 16 8.011873154825896 9 17 7.608979867079665 9 18 7.867053239545857 9 19 6.738571555814779 9 20 6.412981750889983 9 21 3.93034912068444 9 22 5.202135843306351 9 23 5.003023252590047 9 24 5.05683031736784 9 25 4.581221463957277 9 26 4.356458887118215 9 27 3.360387745611021 9 28 3.791179482597663 9 29 4.166409471903798 9 30 4.70197359006454 9 31 5.249782318693121 9 32 4.738111693722082 9 33 5.042188077994595 9 34 5.444386246437453 9 35 4.214306086065918 9 36 2.584635771187866 9 37 3.1980468562601025 9 38 3.567114716498075 9 39 5.610392850434462 9 40 -2.5529830983542716 9 41 -2.113345761615829 9 42 7.66179918083963 9 43 11.632250850155984 9 44 10.989320338133702 9 45 7.875874496516584 9 46 10.941519810147245 9 47 12.286259074943413 9 48 11.68554132818634 9 49 14.279425767554816 9 50 11.465508590037981 9 51 10.238956834465185 9 52 11.563959900160938 9 53 10.896426474120673 9 54 11.958402987389988 9 55 10.667158614325174 9 56 11.62087356331806 10 1 2.3086837211189817 10 2 2.958030486731289 10 3 3.1351263402885294 10 4 4.148547601133501 10 5 3.2809117811387267 10 6 5.1684114418830305 10 7 2.9421596707375812 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-10.290891142564293 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 305.0 5 49.0 6 10.0 7 27.0 8 32.0 9 47.0 10 30.0 11 30.0 12 27.0 13 49.0 14 62.0 15 102.0 16 124.0 17 158.0 18 237.0 19 274.0 20 336.0 21 396.0 22 502.0 23 600.0 24 722.0 25 696.0 26 750.0 27 774.0 28 809.0 29 851.0 30 963.0 31 1138.0 32 1366.0 33 1692.0 34 2064.0 35 2287.0 36 2670.0 37 2512.0 38 1559.0 39 432.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 0.0 0.0 0.0 100.0 2 0.0 0.0 0.0 100.0 3 0.0 0.0 100.0 0.0 4 0.0 100.0 0.0 0.0 5 100.0 0.0 0.0 0.0 6 0.0 100.0 0.0 0.0 7 99.80552629446561 0.14180374361883152 0.03646381978769954 0.01620614212786646 8 99.7325986548902 0.17421602787456447 0.04051535531966616 0.05266996191556599 9 99.48119325551232 0.29588197146562906 0.08916990920881972 0.13375486381322957 10 61.028279718013124 28.291872619722874 4.006968641114982 6.672879021149016 11 38.77319504092051 23.24771088242444 20.45620290089944 17.52289117575561 12 36.439510574507736 12.936552953569402 22.036301758366424 28.587634713556437 13 26.869783648002592 12.98922291548497 25.346406287983147 34.794587148529295 14 21.06820115897394 14.462860153179074 29.476840782915264 34.99209790493172 15 24.572008113590265 16.450304259634887 30.2920892494929 28.685598377281945 16 32.87937743190661 17.088197146562905 23.982652399481193 26.049773022049287 17 30.52729704535322 21.237790297085883 23.924938191545415 24.309974466015483 18 31.034762174864273 23.077546390081842 22.52653755773438 23.361153877319506 19 29.117873495684588 25.653389521455487 20.961141051096075 24.267595931763847 20 28.805933852140075 25.701199740596625 22.43433852140078 23.058527885862517 21 30.035249787285768 22.965033831692395 23.046067825452777 23.953648555569064 22 27.663884612268046 22.56705291305405 26.76849525970343 23.000567214974478 23 28.31213029738271 22.020095616238557 27.663884612268046 22.00388947411069 24 29.414147962077624 21.015314804310833 23.13831942306134 26.432217810550195 25 28.745644599303137 23.280123166680173 22.461712989222914 25.512519244793776 26 23.875629160578015 23.481896411755155 26.039129728852085 26.60334469881474 27 23.097187322687848 18.37967090864878 27.86739077571533 30.655750992948043 28 21.863333874371044 23.14965103067684 27.292647297516638 27.694367797435483 29 24.721377912867272 21.673758865248228 28.45390070921986 25.15096251266464 30 25.581583853448976 20.227770122396045 31.57169490151577 22.618951122639217 31 26.611167512690354 20.584771573604062 31.11472081218274 21.68934010152284 32 30.927876823338735 17.159643435980552 28.3387358184765 23.573743922204212 33 29.723146870179427 18.6490216773565 30.547211171551513 21.08062028091256 34 24.458593559899423 21.262876145672806 36.75075026360613 17.527780030821642 35 22.168625861370085 23.72111876773409 35.58167815160114 18.52857721929469 36 23.002637451815783 26.394806248731996 31.044836680868332 19.557719618583892 37 24.91879161929511 25.3288939418548 29.990254994315414 19.762059444534678 38 25.80265454397857 26.35466980557698 29.321751836668426 18.520923813776026 39 25.144367629117525 19.568930459536396 26.888979259861735 28.39772265148434 40 16.363413838120106 14.360313315926893 21.76892950391645 47.50734334203655 41 15.672007848266842 17.008665794637018 24.595323741007196 42.724002616088946 42 19.442075770495798 24.314776464855647 30.917285905713243 25.325861858935315 43 12.6056566970091 13.170513654096228 25.459200260078024 48.764629388816644 44 14.469505740574872 14.164156013353962 23.410145753603125 47.95619249246804 45 24.10674453502048 20.65944762136513 31.605629233077824 23.628178610536562 46 22.017753637874428 23.387783227270884 31.449880426411575 23.144582708443114 47 18.99416437023829 19.877613875830765 30.446587777597667 30.681633976333277 48 21.399043527599904 21.89754397341331 34.70454729674962 21.998865202237173 49 16.851558372952987 13.820147100654232 24.34475192002926 44.98354260636353 50 21.097080677250396 19.680051971253402 34.98314994518657 24.239717406309637 51 21.08578162460888 21.35397618757365 35.503271160957375 22.056971026860094 52 20.124380131696608 22.08763515161369 33.867165271116164 23.920819445573528 53 20.944766851994643 21.626557363743355 36.66653139077148 20.762144393490523 54 18.473146195034886 17.122343014765537 32.82492292714587 31.57958786305371 55 21.057969250740335 19.532676159182184 36.07155896312523 23.337795626952254 56 19.904232439232235 20.02597086393702 33.311690946719146 26.758105750111593 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 1.0 10 2.5 11 4.0 12 4.0 13 19.0 14 34.0 15 46.5 16 59.0 17 68.0 18 77.0 19 83.5 20 90.0 21 109.5 22 141.5 23 154.0 24 191.0 25 228.0 26 265.0 27 302.0 28 368.5 29 469.0 30 503.0 31 560.5 32 618.0 33 581.5 34 545.0 35 499.5 36 454.0 37 448.0 38 429.5 39 417.0 40 423.0 41 429.0 42 441.0 43 453.0 44 500.0 45 547.0 46 556.0 47 620.5 48 676.0 49 695.5 50 715.0 51 805.5 52 896.0 53 959.5 54 1098.0 55 1173.0 56 1388.5 57 1604.0 58 1724.0 59 1844.0 60 1960.0 61 2076.0 62 2127.0 63 2013.5 64 1849.0 65 1601.5 66 1354.0 67 1142.5 68 931.0 69 806.5 70 682.0 71 566.5 72 380.5 73 310.0 74 241.5 75 173.0 76 158.0 77 143.0 78 102.0 79 46.5 80 32.0 81 23.5 82 15.0 83 9.5 84 4.0 85 2.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 1.0 95 2.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.04051535531966616 10 0.0 11 0.0 12 0.0 13 0.0 14 0.02025767765983308 15 0.12964913702293168 16 0.04051535531966616 17 0.03646381978769954 18 0.0 19 0.012154606595899845 20 0.04051535531966616 21 0.004051535531966615 22 0.0 23 0.0 24 0.0 25 0.0 26 0.1863706344704643 27 0.03241228425573292 28 0.1539583502147314 29 0.02836074872376631 30 0.03241228425573292 31 0.2309375253220971 32 0.00810307106393323 33 0.19447370553439752 34 0.09723685276719876 35 0.04861842638359938 36 0.14990681468276476 37 0.21878291872619726 38 0.18231909893849768 39 0.3727412689409286 40 0.6887610404343246 41 0.8832347459687221 42 0.22283445425816387 43 0.2998136293655295 44 0.48618426383599384 45 0.10128838829916538 46 0.04456689085163277 47 0.02430921319179969 48 0.03241228425573292 49 0.2957620938335629 50 0.2147313831942306 51 0.2957620938335629 52 0.32412284255732926 53 0.16611295681063123 54 0.12154606595899846 55 0.12559760149096508 56 0.158009885746698 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 24682.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.48075520622317 #Duplication Level Percentage of deduplicated Percentage of total 1 93.23823638384587 81.56551332955189 2 4.441459799925899 7.770845150311969 3 1.12078547610226 2.941414796207763 4 0.4168210448314191 1.4585527915079814 5 0.23619859207113744 1.0331415606514869 6 0.20841052241570954 1.0939145936309862 7 0.07410151908114117 0.45377197958026094 8 0.041682104483141906 0.2917105583015963 9 0.03241941459799926 0.2552467385138968 >10 0.18988514264542425 3.1358885017421603 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC 47 0.19042217000243092 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC 47 0.19042217000243092 No Hit CCTAGAGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC 44 0.17826756340653108 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGACCACCCGACTGCTCTTC 43 0.17421602787456447 No Hit CCTAGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCTGTCGACCGTGCGCTT 38 0.1539583502147314 No Hit CCTAGAGGGGATCCCCTCGACCACTCCTTTGGCGCTTCGCCGTCGACCGTGCGCTT 28 0.11344299489506524 No Hit CCTAGAGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 26 0.10533992383113198 No Hit CCTAGAGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC 25 0.10128838829916538 No Hit CCTAGAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACCGCTCTTC 25 0.10128838829916538 No Hit CCTAGAGGGGCTCTTTTCCTCTGGCGCGCCACCGACGATCCTATTGTCATCATGGG 25 0.10128838829916538 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 14 0.0 0.0 0.0 0.0 0.0 15 0.0 0.0 0.0 0.0 0.0 16 0.0 0.0 0.0 0.0 0.0 17 0.0 0.0 0.0 0.0 0.0 18 0.00810307106393323 0.0 0.0 0.0 0.0 19 0.00810307106393323 0.0 0.0 0.0 0.0 20 0.00810307106393323 0.0 0.0 0.0 0.0 21 0.012154606595899845 0.0 0.0 0.0 0.0 22 0.012154606595899845 0.0 0.0 0.0 0.0 23 0.01620614212786646 0.0 0.0 0.0 0.0 24 0.01620614212786646 0.0 0.0 0.0 0.0 25 0.01620614212786646 0.0 0.0 0.0 0.0 26 0.02836074872376631 0.0 0.0 0.0 0.0 27 0.02836074872376631 0.0 0.0 0.0 0.0 28 0.02836074872376631 0.0 0.0 0.0 0.0 29 0.02836074872376631 0.0 0.0 0.0 0.0 30 0.02836074872376631 0.0 0.0 0.0 0.0 31 0.03241228425573292 0.0 0.0 0.0 0.0 32 0.03241228425573292 0.0 0.0 0.0 0.0 33 0.03241228425573292 0.0 0.0 0.0 0.0 34 0.03241228425573292 0.0 0.0 0.0 0.0 35 0.03241228425573292 0.0 0.0 0.0 0.0 36 0.03241228425573292 0.0 0.0 0.0 0.0 37 0.03241228425573292 0.0 0.0 0.0 0.0 38 0.03241228425573292 0.0 0.0 0.0 0.0 39 0.03241228425573292 0.0 0.0 0.0 0.0 40 0.03241228425573292 0.0 0.0 0.0 0.0 41 0.03241228425573292 0.0 0.0 0.0 0.0 42 0.03241228425573292 0.0 0.0 0.0 0.0 43 0.03241228425573292 0.0 0.0 0.0 0.0 44 0.03241228425573292 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCCTTT 25 1.921807E-5 50.4538 38 GACCGCT 15 0.008172253 50.247444 46 TCTTGTC 15 0.008172253 50.247444 42 CCTCATG 15 0.008172253 50.247444 48 CTCATGG 15 0.008172253 50.247444 49 TGCGGAG 15 0.008172253 50.247444 44 CCAACAC 20 4.0454313E-4 50.144897 43 ATGTCGT 15 0.008305571 50.04277 50 TTCGTGG 20 4.127894E-4 49.94106 15 GGCTCTC 20 4.127894E-4 49.94106 9 CGCGCAG 20 4.127894E-4 49.94106 32 GCTGGCG 15 0.008372833 49.941055 13 ATTCGTG 15 0.008372833 49.941055 14 ATTCAAG 15 0.008372833 49.941055 33 CGCCGGT 15 0.008372833 49.941055 32 AGGGGAC 95 0.0 49.83976 6 AGGGTGG 35 5.140282E-8 49.83976 6 AGGGGTC 45 1.2914825E-10 49.839756 6 AGGGGTA 15 0.0084405 49.839756 6 GCCTCGC 15 0.0084405 49.839756 28 >>END_MODULE