FastQCFastQC Report
Fri 17 Jun 2016
SRR1526363_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526363_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42453
Sequences flagged as poor quality0
Sequence length56
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA2000.4711092266742044No Hit
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA740.17431041386945564No Hit
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA740.17431041386945564No Hit
CTTCTGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC650.1531104986691164No Hit
CTTCTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA640.15075495253574542No Hit
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAACAAAAAAA630.1483994064023744No Hit
CTTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA510.12013285280192212No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTCTC150.00794585550.66906740
AACAACC309.33891E-750.66906738
CTCCCGG150.00794585550.66906740
CGACCCT150.00794585550.66906740
CCCGCCG203.8746605E-450.66906439
TGCTTGT150.00809783750.4272137
TACAAAC203.967452E-450.42720437
TCAAAGG150.00817463350.30714436
TTGCCTG150.00817463350.30714436
AAAAACT150.00821323450.24732635
ACAACAC204.0381972E-450.24732643
AAACAAG150.0082519750.1876546
TCTCGTT150.0082519750.1876546
GCCTGCA204.0620004E-450.1876547
CAGCCGG150.0083298550.0687250
ACTCAAA150.00836899450.00946834
TGATACC204.1340798E-450.00946434
CCGCATA150.00840827549.9503611
AACCCCC150.00840827549.9503610
TACCTGG204.1583306E-449.95035610