##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526363_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	42453
Sequences flagged as poor quality	0
Sequence length	56
%GC	48
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	38.3917273219796	39.0	38.0	40.0	35.0	40.0
2	37.994323133818575	39.0	38.0	40.0	35.0	40.0
3	37.4771865356983	39.0	38.0	40.0	34.0	40.0
4	37.622900619508634	39.0	38.0	40.0	34.0	40.0
5	37.873318728947304	39.0	38.0	40.0	35.0	40.0
6	37.315760959178384	39.0	37.0	40.0	33.0	40.0
7	37.81242786139966	39.0	38.0	40.0	35.0	40.0
8	37.65503026876782	39.0	38.0	40.0	35.0	40.0
9	37.67323864037877	39.0	38.0	40.0	35.0	40.0
10	37.173203307186775	39.0	37.0	40.0	33.0	40.0
11	35.05427178291287	38.0	33.0	39.0	28.0	40.0
12	33.04190516571267	37.0	30.0	39.0	18.0	40.0
13	32.852943254893646	37.0	30.0	39.0	17.0	40.0
14	32.363437212917816	37.0	29.0	39.0	15.0	40.0
15	32.981532518314374	37.0	31.0	39.0	18.0	40.0
16	32.637693449226205	36.0	30.0	39.0	17.0	40.0
17	32.640190328127574	36.0	30.0	39.0	18.0	40.0
18	32.66852754811203	36.0	30.0	39.0	18.0	40.0
19	33.69964431253386	37.0	31.0	39.0	24.0	40.0
20	33.86267166042447	37.0	31.0	39.0	24.0	40.0
21	35.368713636256565	38.0	35.0	40.0	27.0	40.0
22	34.853296586813656	38.0	34.0	39.0	25.0	40.0
23	35.216097802275456	38.0	35.0	39.0	27.0	40.0
24	34.74015970602784	38.0	34.0	39.0	25.0	40.0
25	35.12595105175135	38.0	35.0	39.0	27.0	40.0
26	35.40948813982522	38.0	35.0	39.0	28.0	40.0
27	35.37585094104068	38.0	35.0	39.0	28.0	40.0
28	35.332438225802655	38.0	35.0	39.0	28.0	40.0
29	35.08571832379337	38.0	35.0	39.0	27.0	40.0
30	34.93274915789226	38.0	35.0	39.0	27.0	40.0
31	34.44675287965515	38.0	34.0	39.0	24.0	40.0
32	33.41257390525993	38.0	33.0	39.0	17.0	40.0
33	34.52990365816314	38.0	35.0	39.0	25.0	40.0
34	34.294372600287375	38.0	34.0	39.0	24.0	40.0
35	34.254446093326735	38.0	34.0	39.0	25.0	40.0
36	32.04056250441665	35.0	31.0	38.0	17.0	39.0
37	31.79118083527666	35.0	31.0	38.0	15.0	39.0
38	31.13035592302075	35.0	30.0	38.0	9.0	39.0
39	29.027866110757778	34.0	26.0	38.0	4.0	39.0
40	25.319011612842438	31.0	11.0	37.0	4.0	39.0
41	22.006524862789437	26.0	9.0	33.0	4.0	37.0
42	25.453089298753916	30.0	20.0	34.0	4.0	37.0
43	21.577250135443904	26.0	7.0	33.0	4.0	37.0
44	22.586790097284055	27.0	8.0	33.0	4.0	37.0
45	25.12543283160201	30.0	18.0	34.0	4.0	38.0
46	25.020799472357666	30.0	11.0	36.0	4.0	38.0
47	24.27376157162038	30.0	6.0	35.0	4.0	38.0
48	24.19275434009375	29.0	4.0	35.0	4.0	38.0
49	21.60332603114032	26.0	4.0	35.0	4.0	38.0
50	23.934892704873626	29.0	4.0	35.0	4.0	38.0
51	25.358537676960403	32.0	4.0	38.0	4.0	40.0
52	25.06176241961699	31.0	4.0	38.0	4.0	40.0
53	24.627847266388713	31.0	4.0	38.0	4.0	40.0
54	23.859326784915083	30.0	4.0	37.0	4.0	40.0
55	24.039902951499304	31.0	4.0	37.0	4.0	40.0
56	23.839987751160105	31.0	4.0	38.0	4.0	40.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	0.5719369073634653
1	2	0.8292520053343893
1	3	2.069895442370324
1	4	1.4208115774296672
1	5	1.1548355933285421
1	6	1.422983092102264
1	7	0.9143766950906027
1	8	1.3709869589131785
1	9	1.4767170699313965
1	10	1.5043307507404862
1	11	-3.647974803885763
1	12	-7.117279407554108
1	13	-20.96210301226074
1	14	-18.280718496058235
1	15	-11.27777358054437
1	16	-21.972719213368897
1	17	-21.76019533724441
1	18	-20.379119591017982
1	19	-5.99053107351828
1	20	-10.743243807792084
1	21	-7.653945409816881
1	22	-3.6094923679442132
1	23	-3.6780846973048185
1	24	-3.7311717808956004
1	25	-4.185013090672765
1	26	2.7201051525286886
1	27	2.2101179310828414
1	28	2.3262412057501862
1	29	2.5843418757481444
1	30	2.3182330795131563
1	31	2.1424058091660427
1	32	-10.611219519241157
1	33	2.494078791615422
1	34	1.7227785557352462
1	35	1.9445465537541509
1	36	4.821736306998574
1	37	5.408710176735113
1	38	6.103155984274409
1	39	7.929494897162499
1	40	10.562486852801158
1	41	-1.8833857941966627
1	42	-8.343376883508867
1	43	-14.711892056085247
1	44	-16.636852695087555
1	45	-16.212177441109613
1	46	-18.293550447273155
1	47	-18.267965867275613
1	48	-16.51378365710526
1	49	-14.591424791830217
1	50	-16.281948554423955
1	51	-17.76842836459978
1	52	-17.454719822865684
1	53	-17.317733016260153
1	54	-17.914884044977295
1	55	-16.726630486490915
1	56	-16.044003248623724
2	1	0.14221029973928268
2	2	0.5831988670979626
2	3	1.6590367592744428
2	4	0.934481196220581
2	5	0.6685052121194559
2	6	0.8654166693067324
2	7	0.7168410770698088
2	8	0.7587420609539919
2	9	0.7859199963849974
2	10	0.9417585520496843
2	11	-3.0780864711941973
2	12	-10.153860077557958
2	13	-13.341771860932283
2	14	-16.291885226901513
2	15	-5.819552556285608
2	16	-4.0258574497955415
2	17	-1.1352434697049425
2	18	-9.313274385011052
2	19	-2.065232652262985
2	20	-2.516828713452462
2	21	2.719947543590898
2	22	2.6173077860796568
2	23	2.763193700846891
2	24	3.070522481715102
2	25	2.009827109558273
2	26	2.453258791342705
2	27	2.811966217567246
2	28	1.7128411287382477
2	29	2.3610072588825304
2	30	2.466866117634062
2	31	-4.352010825412318
2	32	-11.155308853087902
2	33	-3.268339383201674
2	34	-2.563320789663287
2	35	-4.201006006892751
2	36	2.439372040537272
2	37	1.9639662414251333
2	38	3.140891833331011
2	39	5.026144877909516
2	40	7.262140299085335
2	41	5.642605734490285
2	42	2.064786381797255
2	43	-1.403844308684402
2	44	-10.416213496011698
2	45	2.0115422354402526
2	46	-11.45874105181686
2	47	-11.583714808361483
2	48	-8.334730903928518
2	49	-12.281451746008115
2	50	-8.541478781609168
2	51	-7.446518468565895
2	52	-8.113942002303496
2	53	-7.7335974752512975
2	54	-15.037333024569133
2	55	-8.37199051729569
2	56	-8.054784919782753
3	1	0.8011362794199783
3	2	1.272894077547889
3	3	2.1760178913499146
3	4	1.4653955648707964
3	5	1.13788111923121
3	6	1.7429202977537557
3	7	1.3316451409304761
3	8	1.389511291723224
3	9	1.1313481531339065
3	10	2.0540952719335763
3	11	0.9030456042775015
3	12	1.7941805610487158
3	13	-4.00040756485101
3	14	-5.532523833577855
3	15	-2.9217296245004114
3	16	3.700122230901119
3	17	3.585801523038164
3	18	2.8817909270353965
3	19	1.719673008114377
3	20	1.7952177306694566
3	21	3.4116746843746384
3	22	4.268686595949035
3	23	3.2534694628207674
3	24	4.375892583311618
3	25	3.773542639311536
3	26	3.6651600975836374
3	27	3.188453880847362
3	28	2.8025363391881797
3	29	2.9790043561249107
3	30	3.0799285269228918
3	31	4.406770016387391
3	32	2.6295968072894595
3	33	3.515114898365816
3	34	4.497346844589252
3	35	4.346744355951948
3	36	6.097717468694022
3	37	6.065853033877964
3	38	7.149309830428258
3	39	9.367799449752766
3	40	11.936741169912622
3	41	10.293694268597687
3	42	8.203247920258796
3	43	0.23127905851443842
3	44	4.436616692667545
3	45	8.40980058086841
3	46	1.3061355809843036
3	47	-3.8824085674325985
3	48	1.0702037840250362
3	49	-10.518897319302745
3	50	0.31773747383350326
3	51	-0.19542993445849532
3	52	-0.020182931185935615
3	53	-0.5048601748158852
3	54	-4.160409947646055
3	55	0.04542602842274235
3	56	2.305447300826824
4	1	0.6204835987673007
4	2	0.4251085297623476
4	3	2.3457148610468863
4	4	1.3936006930759248
4	5	1.2942913756414711
4	6	1.6109855658190284
4	7	1.183393392678724
4	8	1.6375299397418672
4	9	1.2630497848355375
4	10	1.5275218453601482
4	11	3.8272880285199236
4	12	2.5874206542888096
4	13	5.295163530720089
4	14	3.412231411177391
4	15	5.930018627247836
4	16	6.126229457008346
4	17	6.51284115007779
4	18	6.319086964331433
4	19	4.959066947508312
4	20	4.802210737662463
4	21	3.444075616775571
4	22	3.827427854690292
4	23	3.668854078205385
4	24	3.9518832593022992
4	25	3.42692259269149
4	26	3.0859060183295526
4	27	2.9043046966981763
4	28	2.388084124735961
4	29	3.1706127477333013
4	30	3.2270147740091346
4	31	2.4247187343361105
4	32	3.2932331709258236
4	33	2.9980985813495025
4	34	2.7425682898106984
4	35	2.2887023979099936
4	36	4.202379473356025
4	37	4.387065155090092
4	38	4.618307499425924
4	39	6.914652596605912
4	40	9.613198046369497
4	41	7.343577718481136
4	42	8.534483351494224
4	43	8.475801203036582
4	44	10.521465177516031
4	45	7.865744636812465
4	46	7.419189194037916
4	47	7.472603087579056
4	48	7.093513807335061
4	49	3.4475362471308237
4	50	7.6429122990083265
4	51	6.618089879061319
4	52	5.977252966249964
4	53	5.869032599076888
4	54	1.4475154602793516
4	55	5.858712741709457
4	56	6.525260820640344
5	1	1.1061295317816544
5	2	0.8804514324736701
5	3	2.0777722773148284
5	4	1.340969114128562
5	5	1.0048176914309437
5	6	1.5089122005877655
5	7	1.1084332650870223
5	8	1.3376894293750468
5	9	1.3324277752661615
5	10	1.4932315742277709
5	11	4.271466656909084
5	12	6.516447767526454
5	13	6.735354918279896
5	14	6.417813548338476
5	15	6.6620760435157855
5	16	6.963550031171021
5	17	6.98094322344302
5	18	6.953855704363328
5	19	5.74774318355297
5	20	5.37956320975178
5	21	3.199259029853721
5	22	3.9175394974335163
5	23	3.0723628501352067
5	24	3.8474175495734286
5	25	3.4053914922130204
5	26	3.329127708919664
5	27	3.224081411211735
5	28	3.0802691327104412
5	29	3.116386272454193
5	30	3.489375220580108
5	31	4.135245050125583
5	32	5.201526631850861
5	33	4.111336220902928
5	34	3.6995060888537594
5	35	4.270361090095001
5	36	5.847515039544227
5	37	6.0026951391411245
5	38	6.769822650941073
5	39	8.319756264867479
5	40	5.232017823084011
5	41	4.568458101256255
5	42	9.713909188814796
5	43	13.650442351361942
5	44	12.0015289733693
5	45	10.660003010018205
5	46	12.171182814452585
5	47	12.44309750544189
5	48	13.064805673363768
5	49	12.932384731979305
5	50	12.340679444143895
5	51	11.338983021007095
5	52	11.249182790811364
5	53	11.084343603861576
5	54	11.050386273676484
5	55	11.704805245696697
5	56	10.84822732781738
6	1	1.0332317701361617
6	2	1.3018065443913969
6	3	2.6518799603154335
6	4	1.8821064402023637
6	5	1.7195787319633098
6	6	2.1904004038545537
6	7	1.3918573425219876
6	8	1.8966730954367534
6	9	1.978828259234703
6	10	2.231283600846041
6	11	4.816838707725779
6	12	6.544578439032801
6	13	6.938319225599919
6	14	7.959253354751059
6	15	6.759172232263513
6	16	7.4956129470814865
6	17	7.425589321446651
6	18	7.346777664435926
6	19	6.019673008114374
6	20	5.2567561922079165
6	21	3.998412084905148
6	22	4.352506224596247
6	23	3.272302354067456
6	24	4.391277198696237
6	25	3.724205769285014
6	26	3.0550805219868167
6	27	2.848400830449485
6	28	2.8280005301696107
6	29	3.314813374692548
6	30	3.3048622139255386
6	31	3.867247469968291
6	32	5.170976117634282
6	33	3.7196241821854485
6	34	3.9233415395494617
6	35	2.429768228630998
6	36	3.384720121213917
6	37	3.093439240774522
6	38	3.3962594325502735
6	39	5.908383004129426
6	40	3.7725501884802597
6	41	3.223932995388136
6	42	6.6165105197282905
6	43	12.404223355596667
6	44	10.450409796115824
6	45	7.950384135245066
6	46	10.11250162872966
6	47	9.496636525405595
6	48	9.401768770762438
6	49	10.713993927381605
6	50	8.38272421123403
6	51	7.910670861297476
6	52	8.155413885790193
6	53	7.6375801434865025
6	54	8.979908354741212
6	55	8.42924565706996
6	56	6.537277539553617
7	1	1.2484297267134323
7	2	1.4857145903684028
7	3	2.690832706164734
7	4	1.9121192115944439
7	5	1.6276247089747997
7	6	1.7389316937651529
7	7	1.3618445711299074
7	8	1.7402235424354728
7	9	1.5829151047008594
7	10	1.8187676366059407
7	11	4.551193752425647
7	12	6.296174863043017
7	13	6.729506965063521
7	14	6.950951949897924
7	15	6.84921054643976
7	16	6.675051005829896
7	17	6.992639129875769
7	18	6.5025886478331145
7	19	5.649302637744
7	20	5.904904340356062
7	21	2.8464325191324775
7	22	4.571535598798036
7	23	3.205891115242423
7	24	3.409795717214756
7	25	2.8595825253514207
7	26	2.7306871631107015
7	27	2.842015134408612
7	28	3.0042457408975807
7	29	1.884417461538014
7	30	3.269102316096678
7	31	2.665459475076851
7	32	2.966633844326495
7	33	2.632778716029634
7	34	2.4412214884638956
7	35	2.367826977034575
7	36	0.5424468134233678
7	37	0.04363081165574556
7	38	0.992044873163298
7	39	2.719366401319718
7	40	-4.120808687637236
7	41	-10.06214618724277
7	42	2.379601196612068
7	43	7.110481337716717
7	44	5.906987063037917
7	45	3.3909971620649912
7	46	6.897303672152393
7	47	7.418731033707001
7	48	7.367924581745836
7	49	9.41386621346079
7	50	6.501498157594185
7	51	8.885766646738084
7	52	8.810586299583296
7	53	8.875766605810895
7	54	9.27748179024568
7	55	7.833460216456931
7	56	8.294621090000614
8	1	1.136680045998233
8	2	1.353530682322429
8	3	2.6001558223844015
8	4	1.864865060892022
8	5	1.6333718354115803
8	6	1.9835038521304185
8	7	1.374615963211646
8	8	1.5001213712988175
8	9	1.5133110178553935
8	10	0.6968008422253504
8	11	4.506493880139573
8	12	6.406647404550046
8	13	6.852112329048197
8	14	6.838563699578639
8	15	6.569517059849719
8	16	6.8059577746677
8	17	6.85662380420527
8	18	6.398501802366958
8	19	5.6058799046660965
8	20	5.636066537035504
8	21	3.774274153870664
8	22	4.576644155630731
8	23	3.49644028510194
8	24	4.443001336627269
8	25	2.5345505968712203
8	26	2.6068046599178487
8	27	2.469090485621898
8	28	2.310759150859262
8	29	2.538951305727032
8	30	2.9083104897876098
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115	11	-1.1525099512780557
115	12	-2.9630843962162388
115	13	-2.5760485904920323
115	14	-5.539788790842813
115	15	-2.511900564671354
115	16	-6.121245290466401
115	17	-6.64624975901312
115	18	-0.6177817225372557
115	19	-0.4525492141078473
115	20	-0.5765771411254192
115	21	1.0223584450584013
115	22	1.5344985617609979
115	23	0.724409633760942
115	24	1.391277198696237
115	25	0.6743973401662373
115	26	1.4621686445921824
115	27	1.6568299492234289
115	28	1.5598012964531378
115	29	1.3196026467231974
115	30	1.537620834615197
115	31	-0.8808368212194466
115	32	-5.959292081599433
115	33	0.11426019751111482
115	34	-1.1513711041286996
115	35	-1.9284693192617226
115	36	-0.1890346680581132
115	37	0.4047419227668563
115	38	0.8531559842744088
115	39	3.071218253171569
115	40	6.277339460510909
115	41	5.058224183127599
115	42	0.7036752706861442
115	43	0.7864072636426442
115	44	0.7310611371119897
115	45	1.752108273176102
115	46	-0.9267704019216794
115	47	-5.646083781107812
115	48	-4.5580013441800915
115	49	-6.262059712465138
115	50	-1.4151685090724797
115	51	-0.7531222421508019
115	52	-0.1338581448611471
115	53	-0.8834926534483643
115	54	-0.8706663579024649
115	55	-0.2776508946541796
115	56	2.0075840529635762
116	1	0.2201646195009559
116	2	0.47694266054384116
116	3	1.4812810492446573
116	4	0.9374603421987331
116	5	0.5399054107291903
116	6	0.6931227269035531
116	7	0.49439817736772085
116	8	0.653478903059252
116	9	0.4289190033363397
116	10	1.0370912233505791
116	11	-1.6495859746698684
116	12	-2.1414469693156555
116	13	-4.755873151895543
116	14	-9.336572416573809
116	15	-3.1010818512210605
116	16	-7.650485056548273
116	17	-9.01905677655698
116	18	-3.1250916640577238
116	19	-3.032958570832996
116	20	-2.506401702528926
116	21	2.3132941175730224
116	22	2.250872830766852
116	23	1.3530646045211725
116	24	1.7596982513278192
116	25	1.5545142992305614
116	26	1.6712329720775614
116	27	1.2767129901591048
116	28	1.8697428169209687
116	29	2.309368728594542
116	30	2.366568203036252
116	31	1.4334906641606722
116	32	-5.438824245342122
116	33	1.4446695542362633
116	34	0.07669907130989628
116	35	1.12123828307746
116	36	2.40014661849159
116	37	1.0816425075621758
116	38	2.7084191421691486
116	39	4.933791352586773
116	40	7.530263437119096
116	41	4.086001960905378
116	42	-0.01416098662379639
116	43	-10.393417297760866
116	44	-1.9107517283850832
116	45	-1.1557864636660042
116	46	-7.31127332589829
116	47	-11.556902494558106
116	48	-8.99659783540816
116	49	-6.471124039950517
116	50	-6.747039854101722
116	51	-4.134701189519223
116	52	-3.715729489890389
116	53	-5.03846341368228
116	54	-8.668911971937554
116	55	-4.935545631496286
116	56	-2.792123549375603
117	1	-0.9136073103236058
117	2	-0.7642854096315972
117	3	-0.5823154419834182
117	4	-0.23139930692407518
117	5	-0.4973752910252003
117	6	-0.3814386766052138
117	7	-1.286303577018245
117	8	-0.9079246057126724
117	9	-0.8013441545584001
117	10	-0.3664175485792427
117	11	-10.763621062389166
117	12	-8.81030661843846
117	13	-11.451048590492032
117	14	-9.09534434639837
117	15	-4.748011675782465
117	16	-9.926800846021958
117	17	-9.437916425679784
117	18	-2.548337278092813
117	19	-2.1469936585522937
117	20	-1.9099104744587514
117	21	1.05013622283618
117	22	1.2706096728721121
117	23	0.3771874115387206
117	24	-5.358722801303763
117	25	0.22995289572179445
117	26	1.9343908668144039
117	27	0.7262743936678717
117	28	1.0320235186753592
117	29	1.2501582022787545
117	30	1.3709541679485326
117	31	1.0497187343361105
117	32	-11.320403192710543
117	33	1.0170379752888934
117	34	1.0152955625379718
117	35	0.015975125182720262
117	36	1.0887431097196654
117	37	3.1825197005446313
117	38	2.1864893176077445
117	39	5.543440475393794
117	40	4.041228349399798
117	41	6.461001960905378
117	42	3.773119715130587
117	43	-4.29692606969069
117	44	1.244950026000879
117	45	5.085441606509434
117	46	-1.3989926241439044
117	47	-4.7849726699967015
117	48	-6.974668010846758
117	49	-1.3592819346873597
117	50	0.7792759353719632
117	51	-1.086455575484134
117	52	-0.8977470337500364
117	53	-2.077937097892807
117	54	-6.953999691235799
117	55	-3.4026508946541796
117	56	-2.2285270581475345
118	1	-0.05742451462468523
118	2	-0.003532721459549748
118	3	1.3760178913499175
118	4	0.21080499415119647
118	5	0.36418384875975107
118	6	0.32555057070661064
118	7	0.1921910466376744
118	8	0.532935609341088
118	9	0.7268816518932155
118	10	-0.15136378513838622
118	11	-6.935664073141854
118	12	-11.948747478653516
118	13	-10.105618482965152
118	14	-2.7915809055381544
118	15	-15.061183718793217
118	16	-4.78970407182841
118	17	-10.036034705249676
118	18	-2.740541579168081
118	19	-3.125488282208206
118	20	-2.6142115497275675
118	21	0.9694910615458596
118	22	1.081093543839856
118	23	1.0882089169150646
118	24	-4.834529252916667
118	25	1.186942143033619
118	26	1.5311650603627882
118	27	1.6926722431302395
118	28	-0.16421304046442486
118	29	1.9154807829239147
118	30	1.303749866873261
118	31	-1.8279694377069013
118	32	-9.634919321742803
118	33	0.03719926561147702
118	34	0.2948654550110845
118	35	-0.8388635844946961
118	36	4.037667840902458
118	37	2.975530453232807
118	38	3.5225108229840885
118	39	6.524623271092715
118	40	7.324830499937434
118	41	7.295679380260218
118	42	2.5163992850230628
118	43	0.6278051131050084
118	44	3.455971531377223
118	45	2.115011498982554
118	46	-4.587164667154656
118	47	-6.064542562469821
118	48	-7.810689516223102
118	49	-3.282669031461552
118	50	-4.349756322692553
118	51	-3.922477080860478
118	52	-4.221671764932832
118	53	-6.473098388215387
118	54	-10.876042701988487
118	55	-8.858296055944502
118	56	-5.2836345850292545
119	1	-0.44194064365694175
119	2	-0.08095207629826007
119	3	1.0560178913499172
119	4	0.2069340264092574
119	5	-0.019041957691861455
119	6	0.23522799006145334
119	7	-0.3329702436849118
119	8	-0.28125793904600727
119	9	-0.05634415455840269
119	10	-0.41975088191257726
119	11	-7.3769543957225
119	12	-4.571973285105127
119	13	-1.3727152571586991
119	14	-2.098677679731704
119	15	-3.746345009115796
119	16	-6.016800846021958
119	17	-6.229583092346452
119	18	-2.136670611426144
119	19	-0.4803269918856259
119	20	-0.583243807792087
119	21	0.5501362228361799
119	22	0.7056096728721144
119	23	-0.05114592179461397
119	24	-5.048722801303764
119	25	0.6966195623884559
119	26	1.3943908668144047
119	27	2.4746077270012066
119	28	1.1686901853420224
119	29	2.185158202278757
119	30	1.827620834615196
119	31	1.3113854010027737
119	32	-8.170403192710545
119	33	1.6720379752888945
119	34	1.8419622292046327
119	35	2.395975125182723
119	36	5.685409776386329
119	37	5.745853033877964
119	38	6.903155984274406
119	39	7.460107142060458
119	40	8.982895016066465
119	41	8.066001960905378
119	42	0.42478638179725436
119	43	-2.4302594030240243
119	44	-4.203383307332452
119	45	-3.9378917268238993
119	46	-6.272325957477236
119	47	-9.25163933666337
119	48	-9.871334677513424
119	49	-4.174281934687359
119	50	-3.9807240646280384
119	51	-6.703122242150801
119	52	-7.269413700416704
119	53	-7.467937097892808
119	54	-11.797333024569133
119	55	-12.557650894654179
119	56	-8.546860391480866
120	1	-0.02819064365694146
120	2	0.5377979237017385
120	3	1.6260178913499175
120	4	0.8831840264092605
120	5	0.6172080423081354
120	6	0.9102279900614505
120	7	0.35952975631509076
120	8	0.22749206095399188
120	9	0.6674058454415999
120	10	1.1127491180874216
120	11	-6.2219543957224985
120	12	-3.883223285105128
120	13	-6.4927152571587
120	14	-4.7411776797317025
120	15	-4.456345009115797
120	16	-7.864300846021958
120	17	-3.917083092346452
120	18	-0.131670611426145
120	19	-1.1365769918856259
120	20	-1.4307438077920835
120	21	1.73763622283618
120	22	2.583109672872112
120	23	1.168854078205385
120	24	-2.858722801303763
120	25	1.0841195623884587
120	26	3.121890866814404
120	27	2.7158577270012074
120	28	1.8236901853420235
120	29	2.8751582022787545
120	30	3.100120834615197
120	31	-3.002364598997225
120	32	-9.664153192710543
120	33	-3.9204620247111066
120	34	-8.974287770795364
120	35	-1.8277748748172797
120	36	-1.2445902236136703
120	37	-0.515396966122033
120	38	-1.9280940157255912
120	39	1.0538571420604583
120	40	-5.073354983933534
120	41	-0.6327480390946221
120	42	-3.466463618202745
120	43	-5.099009403024024
120	44	-6.557133307332453
120	45	-10.372891726823898
120	46	-10.857325957477236
120	47	-11.70163933666337
120	48	-10.360084677513424
120	49	-14.23428193468736
120	50	-9.439474064628037
120	51	-9.596872242150802
120	52	-8.595663700416704
120	53	-8.859187097892807
120	54	-10.037333024569133
120	55	-9.84015089465418
120	56	-6.561860391480867
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	82.0
5	26.0
6	86.0
7	59.0
8	131.0
9	180.0
10	103.0
11	115.0
12	88.0
13	83.0
14	98.0
15	124.0
16	185.0
17	251.0
18	366.0
19	407.0
20	552.0
21	674.0
22	868.0
23	1080.0
24	1216.0
25	1334.0
26	1389.0
27	1389.0
28	1548.0
29	1595.0
30	1737.0
31	1991.0
32	2341.0
33	2794.0
34	3249.0
35	3802.0
36	4421.0
37	4429.0
38	2686.0
39	974.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	0.0	100.0	0.0
3	0.0	0.0	100.0	0.0
4	0.0	0.0	0.0	100.0
5	0.0	0.0	100.0	0.0
6	100.0	0.0	0.0	0.0
7	99.3098249829223	0.49466468800791463	0.1271994912020352	0.06831083786775964
8	98.78218264904717	0.8432855157468259	0.21199915200339198	0.16253268320260053
9	93.8598558032138	3.4729748833702465	1.5527072239762498	1.114462089439706
10	46.01794926153629	32.59369184745483	10.552846677502178	10.835512213506702
11	30.46898923515417	33.771464914140346	17.7372623842838	18.02228346642169
12	31.24631945916661	20.460273714460698	20.898405295267708	27.395001531104985
13	22.632087249428782	18.667703106965348	24.38461357265682	34.31559607094905
14	20.29641148889046	21.969322117763486	25.590820197450583	32.14344619589548
15	22.857479943369512	22.135441245870695	24.2590844738084	30.74799433695139
16	24.069274269557024	24.210650329877474	24.50518378887842	27.214891611687086
17	24.91693569290949	28.48929000636237	21.295096260338855	25.298678040389284
18	24.955239351677346	32.70825480588014	20.63937052393517	21.69713531850735
19	24.534702916647035	33.923102294680305	17.89803515054422	23.644159638128446
20	24.995876823033246	31.65657470018613	18.787550361661523	24.5599981151191
21	23.870998138943204	30.019552875214966	22.028787486159864	24.08066149968197
22	24.648434739594375	30.183968153016277	22.059689539019622	23.107907568369725
23	26.594115845758836	30.386545120486186	22.026711893152427	20.99262714060255
24	22.78990884036464	30.186323699149646	22.19866676088851	24.8251006995972
25	24.429944407801752	31.725242626966928	20.248751531141053	23.596061434090267
26	22.953295730771952	31.494111816486914	23.349774620631063	22.20281783211007
27	23.758923733006622	28.47112598072709	22.02247720472163	25.74747308154466
28	22.698098069753172	32.663174288545946	22.757090943414035	21.881636698286847
29	23.704052780395855	30.855325164938733	24.516965127238453	20.923656927426958
30	21.22340300195575	32.82122575932515	25.825302198449542	20.130069040269564
31	21.34306155698803	33.0216523812897	26.856508701093247	18.77877736062903
32	23.680738786279683	28.51253298153034	26.486524689031288	21.32020354315869
33	21.74313225715095	31.525535731143208	28.70763711885207	18.023694892853772
34	19.574648684334623	32.11591059134207	31.571253418843725	16.73818730547958
35	20.01037051004054	34.088337890072594	29.867068916753087	16.03422268313378
36	18.373366051625688	35.986975602850265	28.64659525270162	16.993063092822425
37	20.62067499586689	33.610448501452495	28.78297631137668	16.985900191303937
38	21.040217006722493	35.17867673074655	28.45854463969808	15.32256162283288
39	18.86810302012629	27.261547193907713	26.123974174017928	27.746375611948064
40	12.586251867308468	19.853934982097552	21.807791715078366	45.752021435515616
41	14.550126088404625	23.21216158348004	24.139981919398583	38.09773040871675
42	18.290408630580014	33.490710795307	30.273128585255304	17.945751988857676
43	12.136186310438585	17.6592977893368	23.808960870079204	46.39555503014541
44	12.838845616610243	19.612206728378986	22.557352210042854	44.99159544496792
45	18.65258094182564	31.164666210767088	32.298912915320585	17.883839932086683
46	17.536706653154532	32.125097216657636	31.564186561711953	18.774009568475876
47	16.89679547596607	29.201225259189446	28.932610744580582	24.969368520263902
48	18.32469368520264	33.10084825636192	31.892082940622057	16.682375117813383
49	12.93402982957903	20.52851773938119	21.809629612120926	44.72782281891885
50	16.345518088221404	33.84103145366959	32.72409558892982	17.089354869179182
51	17.80183928698078	33.442870989857916	31.232416842005723	17.522872881155582
52	17.222051415462477	33.61871201191968	31.189839888371214	17.969396684246625
53	16.773279830048388	34.21456390888705	31.941461111766788	17.07069514929777
54	16.470865770228826	29.13422977117245	28.15994338287332	26.234961075725405
55	17.8071851484915	32.28599061165758	31.792984690868774	18.113839548982146
56	17.734362096222362	32.96524385927656	30.315471555649935	18.984922488851137
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.5
7	5.0
8	7.5
9	10.0
10	39.5
11	69.0
12	214.0
13	450.0
14	541.0
15	537.5
16	534.0
17	483.5
18	433.0
19	435.0
20	437.0
21	456.5
22	510.0
23	544.0
24	630.5
25	717.0
26	857.0
27	997.0
28	1121.0
29	1350.0
30	1455.0
31	1575.0
32	1695.0
33	1717.0
34	1739.0
35	1657.0
36	1575.0
37	1396.0
38	1173.0
39	1129.0
40	1029.0
41	929.0
42	912.5
43	896.0
44	867.0
45	838.0
46	863.5
47	896.5
48	904.0
49	994.5
50	1085.0
51	1164.0
52	1243.0
53	1315.5
54	1487.5
55	1587.0
56	1683.5
57	1780.0
58	1868.5
59	1957.0
60	2188.5
61	2420.0
62	2447.0
63	2326.5
64	2179.0
65	2049.0
66	1919.0
67	1700.5
68	1482.0
69	1305.0
70	1128.0
71	975.5
72	693.5
73	564.0
74	456.0
75	348.0
76	283.0
77	218.0
78	168.5
79	90.5
80	62.0
81	45.5
82	29.0
83	18.5
84	8.0
85	7.0
86	6.0
87	3.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.025911007467081245
10	0.0
11	0.0
12	0.0
13	0.0
14	0.028266553600452264
15	0.1719548677360846
16	0.03062209973382329
17	0.037688738133936354
18	0.01177773066685511
19	0.016488822933597155
20	0.02355546133371022
21	0.009422184533484089
22	0.0
23	0.0
24	0.0
25	0.002355546133371022
26	0.18844369066968175
27	0.02355546133371022
28	0.17666596000282664
29	0.03062209973382329
30	0.03297764586719431
31	0.24026570560384425
32	0.01177773066685511
33	0.19079923680305277
34	0.09657739146821191
35	0.05888865333427555
36	0.16724377546934258
37	0.2638211669375545
38	0.13662167573551928
39	0.40044284267307373
40	0.6595529173438861
41	0.9869738298824583
42	0.21671024427013402
43	0.3721762890726215
44	0.5040868725413987
45	0.10835512213506701
46	0.05182201493416249
47	0.03062209973382329
48	0.03062209973382329
49	0.3439097354721692
50	0.24733234400395732
51	0.3627541045391374
52	0.40044284267307373
53	0.20728805973664996
54	0.1483994064023744
55	0.14133276800226133
56	0.1695993216027136
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	42453.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.89845240619037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.94703315171722	81.63851789037288
2	3.916944512211651	6.807528325442254
3	0.9433195088232902	2.459190163239347
4	0.4147353012929983	1.4415942336230656
5	0.21143368301211676	0.9186629920146986
6	0.124691659212274	0.6501307328104021
7	0.0840313355560977	0.5111535109415118
8	0.07318858258111735	0.5087979648081408
9	0.03523894716868613	0.2755988976044096
>10	0.23040850071833238	3.396697524321014
>50	0.016264129462470522	0.9210185381480697
>100	0.0027106882437450868	0.4711092266742044
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA	200	0.4711092266742044	No Hit
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAA	74	0.17431041386945564	No Hit
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAAAAAAAAAAAA	74	0.17431041386945564	No Hit
CTTCTGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC	65	0.1531104986691164	No Hit
CTTCTGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA	64	0.15075495253574542	No Hit
CTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAACAAAAAAA	63	0.1483994064023744	No Hit
CTTCTGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCAAAAAAAAAAAAAAA	51	0.12013285280192212	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.004711092266742044	0.0	0.0	0.0	0.0
11	0.016488822933597155	0.0	0.0	0.0	0.0
12	0.016488822933597155	0.0	0.0	0.0	0.0
13	0.016488822933597155	0.0	0.0	0.0	0.0
14	0.023555461333710222	0.0	0.0	0.0	0.0
15	0.030622099733823287	0.0	0.0	0.0	0.0
16	0.0423998304006784	0.0	0.0	0.0	0.0
17	0.04946646880079146	0.0	0.0	0.0	0.0
18	0.06124419946764657	0.0	0.0	0.0	0.0
19	0.07302193013450169	0.0	0.0	0.0	0.0
20	0.07773302240124373	0.0	0.0	0.0	0.0
21	0.0847996608013568	0.0	0.0	0.0	0.0
22	0.09186629920146987	0.0	0.0	0.0	0.0
23	0.10364402986832497	0.0	0.0	0.0	0.0
24	0.10835512213506701	0.0	0.0	0.0	0.0
25	0.12013285280192212	0.0	0.0	0.0	0.0
26	0.1271994912020352	0.0	0.0	0.0	0.0
27	0.14368831413563235	0.0	0.0	0.0	0.0
28	0.15546604480248746	0.0	0.0	0.0	0.0
29	0.1601771370692295	0.0	0.0	0.0	0.0
30	0.16724377546934258	0.0	0.0	0.0	0.0
31	0.1695993216027136	0.0	0.0	0.0	0.0
32	0.17431041386945564	0.0	0.0	0.0	0.0
33	0.17431041386945564	0.0	0.0	0.0	0.0
34	0.17431041386945564	0.0	0.0	0.0	0.0
35	0.17431041386945564	0.0	0.0	0.0	0.0
36	0.17666596000282667	0.0	0.0	0.0	0.0
37	0.17666596000282667	0.0	0.0	0.0	0.0
38	0.1790215061361977	0.0	0.0	0.0	0.0
39	0.1790215061361977	0.0	0.0	0.0	0.0
40	0.1790215061361977	0.0	0.0	0.0	0.0
41	0.1813770522695687	0.0	0.0	0.0	0.0
42	0.1813770522695687	0.0	0.0	0.0	0.0
43	0.18844369066968178	0.0	0.0	0.0	0.0
44	0.19079923680305277	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTCTC	15	0.007945855	50.669067	40
AACAACC	30	9.33891E-7	50.669067	38
CTCCCGG	15	0.007945855	50.669067	40
CGACCCT	15	0.007945855	50.669067	40
CCCGCCG	20	3.8746605E-4	50.669064	39
TGCTTGT	15	0.008097837	50.42721	37
TACAAAC	20	3.967452E-4	50.427204	37
TCAAAGG	15	0.008174633	50.307144	36
TTGCCTG	15	0.008174633	50.307144	36
AAAAACT	15	0.008213234	50.247326	35
ACAACAC	20	4.0381972E-4	50.247326	43
AAACAAG	15	0.00825197	50.18765	46
TCTCGTT	15	0.00825197	50.18765	46
GCCTGCA	20	4.0620004E-4	50.18765	47
CAGCCGG	15	0.00832985	50.06872	50
ACTCAAA	15	0.008368994	50.009468	34
TGATACC	20	4.1340798E-4	50.009464	34
CCGCATA	15	0.008408275	49.95036	11
AACCCCC	15	0.008408275	49.95036	10
TACCTGG	20	4.1583306E-4	49.950356	10
>>END_MODULE