FastQCFastQC Report
Fri 17 Jun 2016
SRR1526362_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526362_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133583
Sequences flagged as poor quality0
Sequence length56
%GC56

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG17311.2958235703644925No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG14191.0622609164339774No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG14161.0600151216846454No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG10020.7500954462768465No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG8620.6452916913080258No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG6130.45889072711348No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG3510.2627579856718295No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG3380.2530262084247247No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG2620.19613274144165052No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC2500.18714956244432301No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG2430.18190937469588195No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC2350.17592058869766364No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG2140.1602000254523405No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG2040.15271404295456756No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG1850.138490676208799No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCAGTCTCAAAGATTAAG1840.13774207795902174No Hit
GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC1660.12426730946303048No Hit
GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAACGATTAAG1460.10929534446748465No Hit
GTTGCCGGGTACCCGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG1440.10779814796793007No Hit
GTTGCCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC1370.10255796021948901No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCG251.9387784E-550.58675839
TAGTCTC251.9387784E-550.58675840
GACACAT150.008071627550.5290841
TGCACGT203.99653E-450.4332337
TATGCTA252.0007386E-550.31870335
TATGCGC150.00820591650.318735
ATATGCG301.0066251E-650.2426334
ACCGGCG252.0277907E-550.20468542
CAAGATT301.0118911E-650.20468547
CAATGAT150.0082798650.20468546
CACGATT1700.050.2046847
CTCCGCA150.00830461850.16679444
CCAATTC150.00830461850.16679443
TGACCGC150.00830461850.16679445
CAAGATA150.00830461850.16679443
ATTCGTG150.00847946949.90315613
GAGTTAA150.00847946949.9031569
GATCTCC150.00847946949.9031569
CCAGTGA150.00847946949.90315613
AACATAT252.1060885E-549.8844331
Produced by FastQC (version 0.11.5)