##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1526362_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 133583 Sequences flagged as poor quality 0 Sequence length 56 %GC 56 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.076087526107365 39.0 38.0 39.0 35.0 40.0 2 37.834941571906604 39.0 38.0 39.0 35.0 40.0 3 37.19606536759917 39.0 38.0 40.0 33.0 40.0 4 37.55694212586931 39.0 38.0 39.0 35.0 40.0 5 37.6820254074246 39.0 38.0 39.0 35.0 40.0 6 37.77226144045275 39.0 38.0 39.0 35.0 40.0 7 37.10847188639273 38.0 37.0 39.0 33.0 40.0 8 37.09788670714088 39.0 37.0 39.0 33.0 40.0 9 37.14796044406848 39.0 38.0 39.0 33.0 40.0 10 37.02799757454167 39.0 37.0 39.0 33.0 40.0 11 35.42625184342319 38.0 35.0 39.0 29.0 40.0 12 33.61885868710839 38.0 32.0 39.0 21.0 40.0 13 33.02892583637139 37.0 31.0 39.0 18.0 40.0 14 32.05489470965617 36.0 29.0 39.0 15.0 40.0 15 32.73303489216442 37.0 30.0 39.0 18.0 40.0 16 32.53026208424725 36.0 30.0 39.0 18.0 40.0 17 32.63255803507931 36.0 30.0 39.0 18.0 40.0 18 32.99881721476535 36.0 30.0 39.0 20.0 40.0 19 33.629818165485126 36.0 31.0 38.0 24.0 40.0 20 33.72514466661177 36.0 31.0 39.0 24.0 40.0 21 35.12961230096644 38.0 35.0 39.0 27.0 40.0 22 34.8410127037123 38.0 34.0 39.0 26.0 40.0 23 35.151658519422384 38.0 35.0 39.0 27.0 40.0 24 34.62610511816624 38.0 33.0 39.0 25.0 40.0 25 34.91279578988345 38.0 34.0 39.0 27.0 40.0 26 35.11544882208065 38.0 35.0 39.0 27.0 40.0 27 35.06679742182763 38.0 35.0 39.0 27.0 40.0 28 34.64446823323327 38.0 34.0 39.0 27.0 40.0 29 34.65619128182478 38.0 34.0 39.0 26.0 40.0 30 34.2665533787982 38.0 33.0 39.0 25.0 40.0 31 34.02033192846395 38.0 33.0 39.0 23.0 40.0 32 33.0057118046458 38.0 32.0 39.0 18.0 40.0 33 34.29654222468428 38.0 33.0 39.0 25.0 40.0 34 34.026403060269644 38.0 33.0 39.0 24.0 40.0 35 34.09899463255055 38.0 33.0 39.0 25.0 40.0 36 32.42548078722592 36.0 31.0 38.0 22.0 39.0 37 32.192644273597686 35.0 31.0 38.0 20.0 39.0 38 31.46840541086815 35.0 30.0 38.0 15.0 39.0 39 29.7099181782113 34.0 27.0 38.0 7.0 39.0 40 25.64097227940681 31.0 13.0 38.0 4.0 39.0 41 22.36050994512775 26.0 10.0 33.0 4.0 37.0 42 26.006220851455648 30.0 21.0 34.0 4.0 37.0 43 21.64729044863493 25.0 9.0 33.0 4.0 36.0 44 23.32523599559824 28.0 10.0 33.0 4.0 37.0 45 25.551769311963348 30.0 20.0 34.0 4.0 37.0 46 25.267960743507782 30.0 16.0 36.0 4.0 38.0 47 24.63482628777614 30.0 11.0 35.0 4.0 38.0 48 24.539612076386966 30.0 11.0 35.0 4.0 38.0 49 22.31401450783408 27.0 6.0 35.0 4.0 38.0 50 24.443477089150566 30.0 10.0 35.0 4.0 38.0 51 25.79135069582207 32.0 4.0 37.0 4.0 39.0 52 25.740962547629564 32.0 4.0 38.0 4.0 39.0 53 25.28311985806577 32.0 4.0 37.0 4.0 39.0 54 24.427382226780352 31.0 4.0 37.0 4.0 39.0 55 24.59987423549404 31.0 4.0 37.0 4.0 39.0 56 24.069222880156907 31.0 4.0 37.0 4.0 39.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1 1 -0.07537381295018264 1 2 0.16457289861822488 1 3 1.7101135145219786 1 4 0.32552268994578526 1 5 0.6324536341564055 1 6 0.5361437115712491 1 7 1.2466370130815676 1 8 1.3009822725504563 1 9 1.4839072782554936 1 10 0.5361667898753311 1 11 -4.848987461343853 1 12 -10.22300826462136 1 13 -21.70339777221177 1 14 -18.858520987838197 1 15 -12.132141334966672 1 16 -21.495706362300304 1 17 -21.709315100932354 1 18 -20.757814155637874 1 19 -7.465628212463827 1 20 -11.83815677745826 1 21 -9.188288436177068 1 22 -3.8398023814914737 1 23 -4.246845140046734 1 24 -4.398501455333744 1 25 -4.078824295232472 1 26 0.996478125483705 1 27 1.2277579331398485 1 28 0.9990073894795586 1 29 0.8993319597449201 1 30 1.276470516607148 1 31 0.3295451455574181 1 32 -12.630713025634495 1 33 -0.3765712171868998 1 34 -0.16629317239844 1 35 -0.057479398658372816 1 36 2.449139467559931 1 37 2.864420067395532 1 38 3.2229990445847427 1 39 5.182454288122564 1 40 7.771163740543862 1 41 -5.674404433666311 1 42 -9.5499231832405 1 43 -14.086886950510069 1 44 -16.73767104869975 1 45 -16.6290328073109 1 46 -18.485638616215535 1 47 -18.019692254983774 1 48 -16.75776551731827 1 49 -15.451638219402119 1 50 -16.988680292931498 1 51 -18.15514885608341 1 52 -17.978873960155724 1 53 -17.48854219620308 1 54 -18.021774533183702 1 55 -17.26859492162741 1 56 -16.54134500331285 2 1 0.38452006969650654 2 2 0.6063644092299612 2 3 2.129979307281026 2 4 0.862585736138044 2 5 0.8639611247931143 2 6 0.6760469575138259 2 7 1.376084578624635 2 8 1.5656514360960259 2 9 1.6588448562829612 2 10 0.939334704981448 2 11 -2.4742995712065223 2 12 -9.507574431912246 2 13 -11.205660568716137 2 14 -14.929369926664663 2 15 -7.757063307501003 2 16 -2.8078942524376345 2 17 -2.1944118549897844 2 18 -9.668862844776449 2 19 -1.8978223447984064 2 20 -3.0018584004295477 2 21 2.4812652467193104 2 22 2.8258499281215066 2 23 2.2241286427248 2 24 2.3799785696350497 2 25 2.321456603643931 2 26 2.3657821204899463 2 27 2.7810506921910303 2 28 2.495496153524499 2 29 2.801407490331684 2 30 2.75423268889179 2 31 -0.5063630941429551 2 32 -9.64210503562201 2 33 -0.9760562359134966 2 34 -0.2428381162186639 2 35 -0.5978976258743529 2 36 3.8973292303564406 2 37 3.995584237183291 2 38 4.832783688779497 2 39 6.649604725076372 2 40 9.031151256149357 2 41 6.09947197082807 2 42 2.399046854212685 2 43 -3.2695492476386576 2 44 -10.044787153568663 2 45 -1.4162519084344964 2 46 -12.419315270397808 2 47 -13.118162916656683 2 48 -10.02945715277395 2 49 -11.609721864845314 2 50 -10.31304046771302 2 51 -9.614106409142089 2 52 -9.545977580630131 2 53 -9.881488513306703 2 54 -15.269745819076334 2 55 -9.84233087668359 2 56 -9.423913667482637 3 1 0.39645158387521207 3 2 0.5713189303642565 3 3 2.05753414944261 3 4 0.5352449121680039 3 5 0.8128107770135529 3 6 0.9716595845871225 3 7 1.6865179654625209 3 8 1.7787600503282306 3 9 1.859700929049147 3 10 1.3292223454308854 3 11 0.8109331735767853 3 12 0.22361871950562318 3 13 -6.107961264275261 3 14 -7.43391781323502 3 15 -3.562895303220639 3 16 2.254492050398106 3 17 1.9821531530359024 3 18 1.4064715586478442 3 19 0.6056019462663329 3 20 0.48128766698618364 3 21 2.965481405092774 3 22 3.673094443905349 3 23 3.0555358123342202 3 24 3.122728703396419 3 25 2.7461757047675306 3 26 2.110565427071002 3 27 2.2835119013938154 3 28 2.1103169132890827 3 29 2.4396097375227015 3 30 2.011589564226192 3 31 2.7337118122240867 3 32 1.5569853870639179 3 33 2.3549764018607178 3 34 2.4307306371253716 3 35 2.3849809188019435 3 36 4.122155340575809 3 37 3.9586660991415634 3 38 5.108118092203785 3 39 7.466779684947966 3 40 9.917989137369261 3 41 8.786309852047975 3 42 6.208410150092831 3 43 1.5879146367915205 3 44 2.0432813322526293 3 45 6.44656243078434 3 46 -0.15746401304093283 3 47 -5.601041461332979 3 48 -0.35101948557224105 3 49 -10.646479489243388 3 50 -0.5503866421378447 3 51 -2.3007837767183332 3 52 -2.0637945950763594 3 53 -2.9877485454094312 3 54 -5.030901517310685 3 55 -2.964428254960744 3 56 -0.06297198743983401 4 1 0.09877148619511189 4 2 0.31735067639600345 4 3 1.8944083863168473 4 4 -0.15364397672088614 4 5 0.787902352105128 4 6 0.9576180705456139 4 7 0.9084959874405385 4 8 1.4120933836615635 4 9 1.32258249193071 4 10 0.6916155078240536 4 11 2.6093458719894826 4 12 2.412141308028211 4 13 4.099486843172848 4 14 3.2333593540421433 4 15 5.10450396417863 4 16 5.693930389836446 4 17 5.557244728127472 4 18 4.569108921285206 4 19 3.265675206339594 4 20 3.3829970686955875 4 21 2.406262845874217 4 22 2.309236080046986 4 23 2.114265971064377 4 24 2.6810925617602734 4 25 2.3795090381008634 4 26 2.0696618861674665 4 27 1.7400442579261721 4 28 1.2677039706761377 4 29 1.5985840964970564 4 30 1.6733722260088584 4 31 1.668754547266822 4 32 2.76992800000653 4 33 1.4658433127276282 4 34 1.491292297687032 4 35 1.2811958150168365 4 36 2.7739257923462617 4 37 2.8670910075664686 4 38 3.425456309542003 4 39 5.540894459062741 4 40 7.897232116612244 4 41 2.683501549239672 4 42 5.540888782571464 4 43 4.946766895643776 4 44 7.783696472667771 4 45 6.047783432005339 4 46 6.45560070002378 4 47 5.9728291125375925 4 48 5.377918243365489 4 49 0.5131053703414707 4 50 5.685999194247991 4 51 3.60019302425847 4 52 3.7924935612117956 4 53 2.432397974737089 4 54 0.4258750394658719 4 55 3.8184777279452398 4 56 3.29893277446493 5 1 1.0756495788626808 5 2 1.0740758225948355 5 3 2.719689537913787 5 4 1.2071747367294066 5 5 1.4348659148581646 5 6 1.4781758753139442 5 7 1.574487890274547 5 8 2.092795138047528 5 9 1.8486733601268384 5 10 1.1555381349045746 5 11 3.032737684854979 5 12 5.596801676899112 5 13 5.604570064045539 5 14 6.041771409822623 5 15 6.724072115325729 5 16 6.527173754658762 5 17 5.697190747020862 5 18 5.751834967169145 5 19 5.372017986366586 5 20 4.649708719617763 5 21 3.5043285228872563 5 22 3.292142062952969 5 23 2.8891197722339683 5 24 3.804934217180879 5 25 3.025123073188581 5 26 2.4481594120334123 5 27 2.25970237758429 5 28 1.907560021058508 5 29 2.418557105943748 5 30 2.114847709589604 5 31 2.8635363736275963 5 32 3.7647548106228115 5 33 2.8183849231639755 5 34 2.7580489328647175 5 35 2.416058613037535 5 36 3.3181453155131493 5 37 2.715808956284416 5 38 4.453481500725093 5 39 5.502118030812625 5 40 5.679643272707608 5 41 0.23217451370210895 5 42 7.225954009741955 5 43 11.487162757092271 5 44 10.237767547791478 5 45 7.89267771900489 5 46 9.751057290217215 5 47 9.357354528641956 5 48 9.493842669816232 5 49 9.088858856621272 5 50 9.6175331573609 5 51 9.026283892454597 5 52 8.730190367329655 5 53 9.58894318391388 5 54 8.350301490208114 5 55 9.679431394162066 5 56 8.537529265693 6 1 0.9003206314942602 6 2 1.079503454173782 6 3 2.5764329589664214 6 4 0.9852449121680067 6 5 1.3199536341564055 6 6 1.433713156015699 6 7 1.8247620130815676 6 8 2.219385050328235 6 9 1.9509211671443865 6 10 1.2740140120975525 6 11 3.37670698310059 6 12 5.629422290934194 6 13 5.742782783343785 6 14 7.191826234384024 6 15 6.793205887255549 6 16 6.408807526588582 6 17 6.0736710101787565 6 18 5.5026025110287975 6 19 4.702774565313952 6 20 4.517746000319519 6 21 2.9297671193784893 6 22 3.422350396286305 6 23 2.8000298599532627 6 24 3.582401322444035 6 25 3.0670090381008634 6 26 2.0242559032614835 6 27 2.2138690442509557 6 28 1.9729657228128943 6 29 2.3489847375226987 6 30 2.8025121832738122 6 31 2.9649618122240895 6 32 3.961300863254394 6 33 2.3994704494797645 6 34 2.88058182760156 6 35 2.2168261568971843 6 36 3.0862922453377095 6 37 2.857475622951082 6 38 3.8636240445847427 6 39 5.048773732567014 6 40 0.6583165183216444 6 41 -0.273362766999643 6 42 5.835493483426166 6 43 10.633599160601044 6 44 8.564412284633583 6 45 5.9334671926891005 6 46 9.038666939340022 6 47 8.634821633905116 6 48 9.204040038237284 6 49 10.586556225042326 6 50 8.884583595957391 6 51 8.525406699472146 6 52 8.592306595399833 6 53 9.10868002601914 6 54 8.573711577927412 6 55 7.569946745039257 6 56 7.45518277446493 7 1 0.8756495788626779 7 2 1.4003916120685176 7 3 2.877584274755897 7 4 1.265069473571515 7 5 1.629602756963422 7 6 1.5939653489981538 7 7 1.9481721008008606 7 8 2.555953032784373 7 9 2.1749891496005276 7 10 1.6871170822729908 7 11 3.369579790118138 7 12 5.317854308478054 7 13 6.172991116677117 7 14 7.015455620348938 7 15 6.424072115325725 7 16 7.085068491500863 7 17 6.397190747020865 7 18 5.993940230327041 7 19 5.314123249524478 7 20 4.9391824038282905 7 21 3.8306443123609455 7 22 3.3605631155845472 7 23 3.1312250353918643 7 24 3.5944079013914063 7 25 3.672491494241214 7 26 2.9639488857176204 7 27 2.9281234302158694 7 28 2.68124423158482 7 29 3.0185571059437493 7 30 2.846426656958023 7 31 3.6793258473118087 7 32 4.222649547464918 7 33 3.255227028427136 7 34 3.036996301285768 7 35 2.742374402511217 7 36 0.881303210249996 7 37 0.7526510615475743 7 38 1.1850604480935125 7 39 3.3021180308126254 7 40 -3.4256198851871282 7 41 -9.709930749455784 7 42 3.0627961150051135 7 43 8.65558380972385 7 44 6.574609653054637 7 45 4.3347829821627855 7 46 7.6563204481119485 7 47 7.325775581273536 7 48 7.283316354026759 7 49 9.215174646094958 7 50 7.675427894203008 7 51 8.421020734559864 7 52 8.803874577855971 7 53 8.704732657598086 7 54 9.68714359547127 7 55 8.21627349942522 7 56 7.716476634114052 8 1 0.8370354556148598 8 2 1.2835706652290568 8 3 2.9268569539412965 8 4 1.253670372804521 8 5 1.6017794298012973 8 6 1.6796040689135197 8 7 1.9626127249743632 8 8 2.4539694305627364 8 9 2.287578411700501 8 10 1.6749509886469696 8 11 3.357810416936438 8 12 5.6781293679861236 8 13 6.202136845320339 8 14 7.165627532541478 8 15 6.770461959282354 8 16 6.842407819720911 8 17 6.502612593093332 8 18 5.606926212871343 8 19 5.137212379384302 8 20 4.752644450905784 8 21 3.5851272533818346 8 22 3.8599812589328693 8 23 3.6354831681609667 8 24 3.9844898902832284 8 25 3.1667787198429025 8 26 2.6579451829934726 8 27 2.708948608740073 8 28 2.2367586457609647 8 29 3.0587052149096365 8 30 2.6457125601582376 8 31 3.28630812713196 8 32 4.027648225063437 8 33 2.4267160524948395 8 34 2.3528651190588477 8 35 2.4832101602472676 8 36 0.5652757042991894 8 37 0.6952059412090392 8 38 1.344753650949901 8 39 2.6795578615452342 8 40 -2.0586799222143704 8 41 -9.678801418590934 8 42 4.158828910561844 8 43 8.58023337333136 8 44 6.505210023327052 8 45 4.082336539422769 8 46 7.560076068318242 8 47 8.629875025864916 8 48 8.406458379945828 8 49 10.536147933585038 8 50 8.342314961116521 8 51 9.823982913877504 8 52 9.639076905282579 8 53 10.250011684310596 8 54 10.566514132366269 8 55 9.729206585910276 8 56 9.473974650511831 9 1 1.0224797224033537 9 2 1.0553557269010554 9 3 2.637512504420968 9 4 1.1916843061073976 9 5 1.516923331126108 9 6 1.2779366408641835 9 7 2.112167316111865 9 8 2.222699444267626 9 9 2.4655991974474176 9 10 1.3417223454308882 9 11 3.2412903164339255 9 12 4.881789715176623 9 13 5.8900365712225735 9 14 6.63453456771736 9 15 6.559777856952518 9 16 6.703788587194644 9 17 6.03815964654239 9 18 5.882337359513642 9 19 4.528058656223045 9 20 4.3506058488043635 9 21 3.0850701496815205 9 22 3.3716875174984224 9 23 3.2739881932865984 9 24 3.2940490497167616 9 25 2.8359484320402544 9 26 2.565922569928148 9 27 2.3710660139479245 9 28 2.3569619349341053 9 29 2.1657461011590655 9 30 1.9147280923647187 9 31 2.5946966607089337 9 32 3.7297667723453003 9 33 2.187822722207038 9 34 2.64620682760156 9 35 2.198833732654755 9 36 0.793205124125592 9 37 1.4805816835571441 9 38 2.321483893069587 9 39 3.929929035597315 9 40 0.45613848801861323 9 41 -3.702813524575401 9 42 5.497425301607983 9 43 10.25528476666165 9 44 7.993389557360857 9 45 5.876649010870917 9 46 9.151356333279413 9 47 9.624878452086936 9 48 9.235290038237284 9 49 10.623488043224143 9 50 9.288466171714969 9 51 9.265937002502444 9 52 9.777439171157404 9 53 9.73368002601914 9 54 9.17835172944256 9 55 8.998450532918042 9 56 8.439084289616446 10 1 0.9558353373766124 10 2 0.9598343365267255 10 3 2.478822664848778 10 4 1.048970402364084 10 5 1.4032869674897412 10 6 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-3.338427438868081 114 53 -3.371460160896749 114 54 -4.791115135468228 114 55 -2.9946463234965712 114 56 0.6182474162094778 115 1 0.1119951597961446 115 2 0.3970152466266157 115 3 1.8727183363249154 115 4 0.571722899589389 115 5 0.799513382584081 115 6 0.5776793509842477 115 7 1.2989168872953982 115 8 1.5473135094477328 115 9 1.4852175507921856 115 10 0.9820525341101316 115 11 -0.42734175903777327 115 12 -3.06407220592115 115 13 0.4956326261110817 115 14 -8.185040903980752 115 15 -2.573048043562057 115 16 -13.10956904573846 115 17 -10.962590782274074 115 18 -3.0801411996630286 115 19 -3.828180623365295 115 20 -3.697957616032685 115 21 -1.1724341384831476 115 22 -0.3021975596885369 115 23 -0.14464388218510038 115 24 -0.12426141340502284 115 25 -0.2179752386286964 115 26 1.0647433246451286 115 27 0.7370608681503299 115 28 1.2053753140078598 115 29 1.2867795488434552 115 30 1.3853541644058893 115 31 0.6852841392681128 115 32 -6.291352438632398 115 33 0.3384445060835404 115 34 0.6721502238279768 115 35 -0.1630873651153948 115 36 2.3244015220672694 115 37 2.6271297110013982 115 38 3.6007506169117853 115 39 5.037669172818582 115 40 7.811520134673842 115 41 6.153425755012936 115 42 1.2323095211620156 115 43 1.497887682613623 115 44 2.3692471902939616 115 45 1.8933728530664595 115 46 -5.421435261917843 115 47 -6.345426007604317 115 48 -5.468719395724978 115 49 -8.311439057976543 115 50 -2.9814737939797133 115 51 -1.0107568225404364 115 52 -0.22363287000897358 115 53 -0.2333011060563308 115 54 -1.004216881192086 115 55 0.1311692293159865 115 56 2.424718937986942 116 1 0.1706761360814184 116 2 0.6318257018802029 116 3 2.0588010782324773 116 4 0.5624620375502687 116 5 0.7840209124438715 116 6 0.4987456483704378 116 7 1.4448403769959413 116 8 1.1857936894719643 116 9 1.554371090691788 116 10 0.8319746390088696 116 11 -1.2810720688871768 116 12 -4.161527632613204 116 13 -7.049027231946734 116 14 -10.461222313016584 116 15 -7.9464252289524 116 16 -8.202956617142302 116 17 -10.472630595325832 116 18 -3.683655134231145 116 19 -4.531313737438342 116 20 -4.252657287142256 116 21 0.8189108502653397 116 22 0.860949402402511 116 23 1.1300661749379728 116 24 0.5702166894165117 116 25 0.8259922185289952 116 26 1.661679450662092 116 27 1.2450234785017216 116 28 1.1681587656263446 116 29 1.5148390953208661 116 30 1.6765087429068402 116 31 -0.7895297779899835 116 32 -8.031961751424504 116 33 -0.0854779450156471 116 34 0.2597389376933066 116 35 0.5420364015455021 116 36 1.8813514961022406 116 37 2.3387447361009315 116 38 3.377624426847735 116 39 4.944368151527257 116 40 8.15062347550819 116 41 4.640962538810754 116 42 0.8977067861784604 116 43 -8.858612230836266 116 44 1.247038431422574 116 45 0.4185589358083668 116 46 -5.86318703619515 116 47 -6.478280430315067 116 48 -8.831797117726019 116 49 -5.187239646517307 116 50 -4.218101801596124 116 51 -0.6955413127602732 116 52 -0.717660912245428 116 53 -2.7135676804028783 116 54 -2.560128253876872 116 55 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1.3764150168904798 117 38 2.0695899536756492 117 39 3.481065399233678 117 40 2.7534869728670976 117 41 1.5036258693639937 117 42 -1.5309837893011071 117 43 -7.076154627277745 117 44 -2.0710043820330846 117 45 -0.5097146254927161 117 46 -5.214173969750888 117 47 -5.20277306306458 117 48 -6.296906931459684 117 49 -4.29886044162434 117 50 -3.6395641313153355 117 51 -0.663987239921795 117 52 -1.491500222781987 117 53 -0.7738957315566175 117 54 -5.823542209951377 117 55 -1.9371555276880166 117 56 2.0716600471922 118 1 -0.22592936850573864 118 2 0.13512845417378117 118 3 1.6670579589664243 118 4 0.20191157883466815 118 5 0.3832869674897381 118 6 0.19975482268236533 118 7 0.9108036797482342 118 8 1.0154267169949023 118 9 0.9044628338110527 118 10 0.7692223454308902 118 11 -5.796209683566076 118 12 -7.63161937573247 118 13 -7.597008883322879 118 14 -0.042965432282638716 118 15 -16.189085779411116 118 16 -7.8759841400780815 118 17 -15.110703989821243 118 18 -4.234480822304537 118 19 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-0.08201632502748168 119 2 0.18208497591291462 119 3 1.6192318720098982 119 4 0.4562594049216244 119 5 0.39241740227235056 119 6 0.11192873572584006 119 7 0.6442819406177946 119 8 1.0062962822122898 119 9 0.870984572941488 119 10 0.8553093019526301 119 11 -9.620122727044336 119 12 -9.119445462688994 119 13 -2.061791492018532 119 14 -2.1616610844565542 119 15 -9.812998822889377 119 16 -12.803375444425907 119 17 -12.31287790286472 119 18 -2.826654735348015 119 19 -3.4514373912077865 119 20 -3.3991471156225117 119 21 -1.8383488226504952 119 22 -1.3491622848731168 119 23 -1.967859632800355 119 24 -7.493006286251617 119 25 -1.5516503821889955 119 26 0.9164660481890152 119 27 1.1423110732364634 119 28 1.175637824262168 119 29 1.0548271288270499 119 30 1.5871589224042495 119 31 -0.5952736950222928 119 32 -10.192911093267345 119 33 -0.27783933312893083 119 34 0.379902479775474 119 35 0.3766993453029812 119 36 2.418946230844959 119 37 2.544069825849636 119 38 3.5662961460340163 119 39 5.6115001818423735 119 40 6.893144416872367 119 41 7.4397260011162984 119 42 3.274352179078342 119 43 1.4798401026300283 119 44 1.552184023764017 119 45 0.9497715405151865 119 46 -8.09493813312375 119 47 -7.407977279138363 119 48 -9.772862135675759 119 49 -6.893063340175065 119 50 -6.158668215636812 119 51 -6.81879619907858 119 52 -7.89795608575959 119 53 -11.548928669633034 119 54 -10.669721393087082 119 55 -11.32022535641002 119 56 -7.389473022636521 120 1 -0.1472337163318258 120 2 0.06251975852160996 120 3 1.5757536111403354 120 4 -0.06547972551315695 120 5 0.2619826196636552 120 6 -0.0837234381871994 120 7 0.8073254188786692 120 8 0.9302093256905479 120 9 0.6101150077240973 120 10 0.05096147586566957 120 11 -9.174470553131293 120 12 -2.9564019844281226 120 13 -6.235704535496794 120 14 -9.009487171413074 120 15 -9.562998822889377 120 16 -15.259897183556344 120 17 -12.128095294169068 120 18 -5.174480822304538 120 19 -4.668828695555614 120 20 -6.203494941709469 120 21 -1.3274792574331045 120 22 -1.1861188066122494 120 23 -1.5656857197568783 120 24 -7.851701938425531 120 25 -1.888606903928121 120 26 0.31863996123249905 120 27 1.0336154210625494 120 28 0.44737695469694927 120 29 0.5656966940444406 120 30 -0.09762368629140639 120 31 -2.214838912413594 120 32 -8.72551978891952 120 33 -2.690882811389802 120 34 -8.326619259354963 120 35 -1.1667789155665886 120 36 -1.4180102908941734 120 37 -1.0211475654547115 120 38 -0.6293560278790267 120 39 0.8071523557554166 120 40 -3.345986017910242 120 41 -1.0820131293184865 120 42 -5.573473907878181 120 43 -4.737551201717798 120 44 -5.697815976235981 120 45 -10.756750198615247 120 46 -13.475372915732445 120 47 -13.46232510522532 120 48 -11.675036048719237 120 49 -15.382193774957674 120 50 -11.245624737375941 120 51 -11.55792663386119 120 52 -10.952303911846546 120 53 -11.831537365285207 120 54 -10.778417045260994 120 55 -11.678921008583933 120 56 -7.639473022636521 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 366.0 5 115.0 6 331.0 7 181.0 8 342.0 9 518.0 10 324.0 11 325.0 12 303.0 13 245.0 14 351.0 15 481.0 16 575.0 17 767.0 18 1046.0 19 1368.0 20 1711.0 21 2084.0 22 2552.0 23 3071.0 24 3560.0 25 3902.0 26 4125.0 27 4429.0 28 4660.0 29 4619.0 30 5393.0 31 6355.0 32 7573.0 33 9183.0 34 11014.0 35 12793.0 36 14779.0 37 14707.0 38 7668.0 39 1767.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 100.0 0.0 0.0 0.0 2 0.0 0.0 100.0 0.0 3 0.0 0.0 100.0 0.0 4 100.0 0.0 0.0 0.0 5 0.0 0.0 0.0 100.0 6 0.0 0.0 0.0 100.0 7 99.02457648054019 0.6108561718182702 0.16544021320078153 0.1991271344407597 8 97.4547659507572 0.8953235067336413 1.5076768750514662 0.14223366745768548 9 93.33223514260256 3.166533136657357 1.1171594797571003 2.3840722409829804 10 39.81794090565416 30.533825411916187 22.258071760628223 7.390161921801427 11 30.480382689154894 31.949154445617268 20.561307371557334 17.00915549367051 12 26.371619143154447 11.39516255810994 20.297492944461496 41.935725354274126 13 24.005300075608424 11.368961619367735 22.889888683440258 41.73584962158359 14 21.923203642136162 12.535567735945127 36.60706262916704 28.93416599275167 15 36.02600440905206 13.991991721779817 24.11031628199937 25.871687587168758 16 41.642452745409344 14.787466674654764 21.22637270467004 22.343707875265853 17 25.81125803021908 18.124709863879364 33.00139265337906 23.0626394525225 18 27.181187955739887 19.49406321589531 32.7324179855361 20.592330842828694 19 42.02707559602252 21.067449383011862 17.17458368276027 19.730891338205343 20 26.404271411347995 35.651757164573645 18.217150046053963 19.726821378024397 21 24.116013206459485 19.98637428783194 35.24193125753345 20.655681248175128 22 22.535970415175697 18.374481591831234 23.749457262205986 35.34009073078708 23 24.642357186168898 20.302733132209937 21.040102408240568 34.01480727338059 24 23.51122523075541 21.733304387534343 32.65535285178503 22.100117529925214 25 37.273455454661146 22.353892336599717 18.98295441785257 21.389697790886565 26 22.469609820846053 21.637207628103695 20.33911015455684 35.55407239649341 27 21.238056728666844 19.11747087189625 21.8191212148441 37.825351184592805 28 22.66223735321025 34.352175412811015 20.458328959011354 22.52725827496738 29 38.000988497483824 21.380002396357536 20.148873711957823 20.470135394200813 30 24.719361965027893 19.44284270041562 35.13011570000374 20.70767963455274 31 24.10771227706875 31.47035211320443 24.241264696393333 20.180670913333483 32 37.9934863175233 18.796840489649234 20.06289072736121 23.146782465466252 33 24.703711482492725 20.592427015512015 23.194515286987308 31.509346215007948 34 20.725291525525346 34.23364009712521 25.71195179711622 19.329116580233222 35 21.3293680520181 24.8456836364726 36.233631977946246 17.59131633356306 36 23.313214620431115 35.78331771321462 22.30253045923149 18.600937207122776 37 23.414634146341466 23.371106941838647 34.666416510318946 18.54784240150094 38 36.283205746290484 23.055640946818322 20.979508596192634 19.681644710698567 39 20.451163979019825 18.726611460947716 19.061001818482392 41.76122274155007 40 14.926575796370209 14.21341968521726 24.178025453723926 46.681979064688605 41 16.836850293793333 15.911665634487862 27.66868570910684 39.58279836261197 42 30.397784831498615 21.332252763332658 25.69505414105941 22.574908264109318 43 12.950261799770127 12.262145330799221 28.120375308938755 46.6672175604919 44 15.288196038009827 13.687148736391475 18.081752725467034 52.94290250013166 45 20.394011859337166 22.380563281033304 35.323882845941064 21.901542013688463 46 19.32202120088399 22.359815709630297 25.610368206165486 32.707794883320226 47 18.098083705883674 28.614112731112257 26.303926193845996 26.983877369158073 48 21.814913299127372 32.863937680236695 24.78184337665256 20.539305643983372 49 14.105365926927266 20.12767075964102 17.66287409410086 48.10408921933085 50 31.370562945956287 21.291857053893654 26.92431779473443 20.413262205415624 51 21.09734752593505 33.03911479030356 26.34896071994651 19.514576963814875 52 20.991514404467527 23.316973445873323 35.781554164255276 19.909957985403874 53 21.559595171466942 21.46656563458898 36.57861370985288 20.3952254840912 54 18.61320980991785 28.16753612760089 23.06769802722312 30.151556035258142 55 20.52262874458095 33.45408997494862 24.578851836851026 21.4444294436194 56 31.476868594408845 21.968457856274664 24.447412253717683 22.107261295598807 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 5.5 9 10.0 10 13.0 11 16.0 12 18.5 13 48.0 14 75.0 15 122.5 16 170.0 17 168.5 18 167.0 19 175.5 20 184.0 21 214.0 22 279.0 23 314.0 24 330.5 25 347.0 26 426.0 27 505.0 28 640.0 29 943.0 30 1111.0 31 1267.0 32 1423.0 33 1528.0 34 1633.0 35 1686.5 36 1740.0 37 1839.5 38 1976.0 39 2013.0 40 2096.5 41 2180.0 42 2274.0 43 2368.0 44 2525.0 45 2682.0 46 3060.0 47 4997.5 48 6557.0 49 8834.5 50 11112.0 51 9928.0 52 8744.0 53 8031.0 54 7362.0 55 7406.0 56 7588.5 57 7771.0 58 7592.5 59 7414.0 60 7722.0 61 8030.0 62 8538.5 63 8775.0 64 8503.0 65 7783.0 66 7063.0 67 6481.0 68 5899.0 69 5326.5 70 4754.0 71 4159.5 72 3125.0 73 2685.0 74 2310.0 75 1935.0 76 1496.5 77 1058.0 78 839.5 79 490.5 80 360.0 81 299.5 82 239.0 83 168.0 84 97.0 85 65.5 86 34.0 87 22.5 88 6.0 89 1.0 90 1.0 91 1.0 92 1.0 93 1.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.022457947493318763 10 0.0 11 0.001497196499554584 12 0.0 13 0.0 14 0.026200938742205217 15 0.16544021320078153 16 0.03817851073864189 17 0.0187149562444323 18 0.006737384247995629 19 0.023206545743096052 20 0.03293832299020085 21 0.008983178997327505 22 7.48598249777292E-4 23 0.0 24 0.0 25 0.0 26 0.1751719904478863 27 0.026200938742205217 28 0.17142899919899987 29 0.03518411773953273 30 0.03593271598931002 31 0.22607667143274218 32 0.013474768495991258 33 0.199875732690537 34 0.11004394271726192 35 0.06812244072973357 36 0.15570843595367673 37 0.24928321717583826 38 0.15720563245323133 39 0.37804211613753247 40 0.5936384120733926 41 0.8811001399878728 42 0.23955143992873346 43 0.3488467843962181 44 0.5015608273507857 45 0.1392392744585763 46 0.07336262847817462 47 0.03293832299020085 48 0.05839066348262878 49 0.320400050904681 50 0.22607667143274218 51 0.34585239139710894 52 0.399751465381074 53 0.2185906889349693 54 0.12501590771280777 55 0.19238975019276405 56 0.22532807318296488 >>END_MODULE >>Sequence Length Distribution pass #Length Count 56 133583.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.63953057305865 #Duplication Level Percentage of deduplicated Percentage of total 1 92.21699839845343 69.75250469715519 2 4.707999918235452 7.122218075066562 3 1.2760017061848992 2.89548510198743 4 0.5559999986653136 1.682223155906622 5 0.28999999501145274 1.0967731744427816 6 0.20199999649220382 0.9167510946257876 7 0.14599999746439885 0.7730359890312417 8 0.10299999821118345 0.6232697210975838 9 0.08299999855852645 0.5650272835678337 >10 0.36799999360888835 5.1155013638142695 >50 0.02599999954845407 1.3781522231065502 >100 0.018999999670024127 2.7517660744575356 >500 0.001999999965265698 1.1156830565763785 >1k 0.003999999930531396 4.211608989164254 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 1731 1.2958235703644925 No Hit GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG 1419 1.0622609164339774 No Hit GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG 1416 1.0600151216846454 No Hit GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG 1002 0.7500954462768465 No Hit GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG 862 0.6452916913080258 No Hit GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG 613 0.45889072711348 No Hit GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTCAG 351 0.2627579856718295 No Hit GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAAAGATTAAG 338 0.2530262084247247 No Hit GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTAAG 262 0.19613274144165052 No Hit GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC 250 0.18714956244432301 No Hit GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAAAGATTAAG 243 0.18190937469588195 No Hit GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCGTCTTCTGCTTGGATC 235 0.17592058869766364 No Hit GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTCAG 214 0.1602000254523405 No Hit GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTAGTCTCAAAGATTAAG 204 0.15271404295456756 No Hit GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCCACGATTCAG 185 0.138490676208799 No Hit GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCAGTCTCAAAGATTAAG 184 0.13774207795902174 No Hit GTTGCCGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC 166 0.12426730946303048 No Hit GTTGCCGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGCCTCAACGATTAAG 146 0.10929534446748465 No Hit GTTGCCGGGTACCCGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG 144 0.10779814796793007 No Hit GTTGCCGGGAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCTGACCCC 137 0.10255796021948901 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 7.48598249777292E-4 0.0 0.0 0.0 0.0 9 0.001497196499554584 0.0 0.0 0.0 0.0 10 0.001497196499554584 0.0 0.0 0.0 0.0 11 0.002994392999109168 0.0 0.0 0.0 0.0 12 0.005988785998218336 0.0 0.0 0.0 0.0 13 0.0074859824977729205 0.0 0.0 0.0 0.0 14 0.011977571996436672 0.0 0.0 0.0 0.0 15 0.012726170246213964 0.0 0.0 0.0 0.0 16 0.015720563245323133 0.0 0.0 0.0 0.0 17 0.020960750993764175 0.0 0.0 0.0 0.0 18 0.02620093874220522 0.0 0.0 0.0 0.0 19 0.02919533174131439 0.0 0.0 0.0 0.0 20 0.03518411773953273 0.0 0.0 0.0 0.0 21 0.039675707238196474 0.0 0.0 0.0 0.0 22 0.04267010023730564 0.0 0.0 0.0 0.0 23 0.046413091486192104 0.0 0.0 0.0 0.0 24 0.05165327923463315 0.0 0.0 0.0 0.0 25 0.053150475734187734 0.0 0.0 0.0 0.0 26 0.05539627048351961 0.0 0.0 0.0 0.0 27 0.05764206523285149 0.0 0.0 0.0 0.0 28 0.06437944948084712 0.0 0.0 0.0 0.0 29 0.0651280477306244 0.0 0.0 0.0 0.0 30 0.0651280477306244 0.0 0.0 0.0 0.0 31 0.0658766459804017 0.0 0.0 0.0 0.0 32 0.0658766459804017 0.0 0.0 0.0 0.0 33 0.0658766459804017 0.0 0.0 0.0 0.0 34 0.0658766459804017 0.0 0.0 0.0 0.0 35 0.0658766459804017 0.0 0.0 0.0 0.0 36 0.06662524423017899 0.0 0.0 0.0 0.0 37 0.06737384247995629 0.0 0.0 0.0 0.0 38 0.06737384247995629 0.0 0.0 0.0 0.0 39 0.06737384247995629 0.0 0.0 0.0 0.0 40 0.06737384247995629 0.0 0.0 0.0 0.0 41 0.06887103897951087 0.0 0.0 0.0 0.0 42 0.07111683372884274 0.0 0.0 0.0 0.0 43 0.07411122672795191 0.0 0.0 0.0 0.0 44 0.0756084232275065 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCACCG 25 1.9387784E-5 50.586758 39 TAGTCTC 25 1.9387784E-5 50.586758 40 GACACAT 15 0.0080716275 50.52908 41 TGCACGT 20 3.99653E-4 50.43323 37 TATGCTA 25 2.0007386E-5 50.318703 35 TATGCGC 15 0.008205916 50.3187 35 ATATGCG 30 1.0066251E-6 50.24263 34 ACCGGCG 25 2.0277907E-5 50.204685 42 CAAGATT 30 1.0118911E-6 50.204685 47 CAATGAT 15 0.00827986 50.204685 46 CACGATT 170 0.0 50.20468 47 CTCCGCA 15 0.008304618 50.166794 44 CCAATTC 15 0.008304618 50.166794 43 TGACCGC 15 0.008304618 50.166794 45 CAAGATA 15 0.008304618 50.166794 43 ATTCGTG 15 0.008479469 49.903156 13 GAGTTAA 15 0.008479469 49.903156 9 GATCTCC 15 0.008479469 49.903156 9 CCAGTGA 15 0.008479469 49.903156 13 AACATAT 25 2.1060885E-5 49.88443 31 >>END_MODULE