FastQCFastQC Report
Fri 17 Jun 2016
SRR1526361_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526361_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27013
Sequences flagged as poor quality0
Sequence length56
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC510.18879798615481436No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC430.15918261577758858No Hit
CACAAGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC380.14067300929182244No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGAGC150.00803216850.480341
GCCCAGT203.9207964E-450.480341
GAGCACT203.9207964E-450.480338
TACGCCC150.00803216850.480338
GCCATGA150.00803216850.480339
CTGTCGA203.9207964E-450.480340
GTACGCC150.00809206850.3857737
GCAAAGT150.008212863550.1977649
TGCTTTT150.008212863550.1977647
CGAAAGC150.008212863550.1977646
AAAGCCA150.008212863550.1977648
AAGCCAT150.008212863550.1977649
GAAAGCC150.008212863550.1977647
TTGCTTT150.008212863550.1977646
ATGAGCA150.008212863550.1977642
CCCAGTT204.0313168E-450.19775842
CCAACAC150.00827376250.10428243
CCAGTTC204.068702E-450.10428243
AGACGAA150.00827376250.10428243
ACGAAAG150.00827376250.10428245