##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1526361_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27013
Sequences flagged as poor quality	0
Sequence length	56
%GC	53
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	37.43930700033317	39.0	38.0	39.0	33.0	40.0
2	36.33435753155888	38.0	37.0	39.0	30.0	40.0
3	36.36682338133491	39.0	36.0	39.0	29.0	40.0
4	35.49827860659683	38.0	37.0	39.0	24.0	40.0
5	35.767630400177694	38.0	36.0	39.0	29.0	40.0
6	35.962647614111724	38.0	35.0	39.0	29.0	40.0
7	36.75478473327657	38.0	36.0	39.0	31.0	40.0
8	36.511050235072005	38.0	36.0	39.0	31.0	40.0
9	36.65412949320697	38.0	36.0	39.0	31.0	40.0
10	35.722837152482136	38.0	35.0	39.0	28.0	40.0
11	34.41368970495687	37.0	32.0	39.0	26.0	40.0
12	32.33065561026173	35.0	29.0	39.0	17.0	40.0
13	32.25191574427128	36.0	29.0	39.0	16.0	40.0
14	31.81927220227298	36.0	28.0	39.0	14.0	40.0
15	32.247769592418464	36.0	29.0	39.0	16.0	40.0
16	32.0134009550957	36.0	29.0	39.0	17.0	40.0
17	32.19957057712953	36.0	29.0	38.0	18.0	40.0
18	32.287602265575835	35.0	29.0	38.0	19.0	40.0
19	33.129900418317106	36.0	30.0	38.0	23.0	40.0
20	33.22226335468108	36.0	30.0	39.0	23.0	40.0
21	34.9132639840077	38.0	34.0	39.0	27.0	40.0
22	34.58882760152519	38.0	33.0	39.0	25.0	40.0
23	34.74464146892237	38.0	33.0	39.0	26.0	40.0
24	34.32758301558509	38.0	33.0	39.0	24.0	40.0
25	34.64191315292637	38.0	33.0	39.0	26.0	40.0
26	35.15170473475734	38.0	35.0	39.0	28.0	40.0
27	35.0215822011624	38.0	35.0	39.0	28.0	40.0
28	34.91985340391663	38.0	35.0	39.0	27.0	40.0
29	34.78040202865287	38.0	34.0	39.0	27.0	40.0
30	34.586939621663646	38.0	34.0	39.0	27.0	40.0
31	34.1998667308333	38.0	33.0	39.0	25.0	40.0
32	33.111686965535114	37.0	32.0	39.0	18.0	40.0
33	34.23166623477585	38.0	33.0	39.0	26.0	40.0
34	33.94932069744197	37.0	33.0	39.0	25.0	40.0
35	34.02295191204235	37.0	33.0	39.0	26.0	40.0
36	31.936363972901937	35.0	31.0	38.0	22.0	39.0
37	31.74201310480139	35.0	30.0	38.0	21.0	39.0
38	31.087772553955503	34.0	30.0	38.0	16.0	39.0
39	28.967978380779623	33.0	26.0	37.0	5.0	39.0
40	25.003775959723097	31.0	12.0	36.0	4.0	39.0
41	21.488061303816682	25.0	9.0	32.0	4.0	36.0
42	25.143042238922	30.0	20.0	33.0	4.0	36.0
43	21.20771480398327	25.0	8.0	33.0	4.0	36.0
44	22.31407100285048	27.0	9.0	33.0	4.0	36.0
45	24.738681375633956	29.0	19.0	33.0	4.0	36.0
46	24.76925924554844	30.0	14.0	35.0	4.0	38.0
47	24.128567726650132	29.0	8.0	35.0	4.0	38.0
48	24.11894273127753	29.0	9.0	35.0	4.0	38.0
49	21.600488653611226	26.0	4.0	35.0	4.0	38.0
50	23.946285121978306	29.0	4.0	35.0	4.0	38.0
51	25.50953244734017	32.0	4.0	37.0	4.0	39.0
52	25.190982119720136	31.0	4.0	37.0	4.0	39.0
53	24.845296708991967	31.0	4.0	37.0	4.0	39.0
54	24.086773035205272	31.0	4.0	37.0	4.0	39.0
55	24.195572502128606	31.0	4.0	36.0	4.0	39.0
56	23.790360196942213	31.0	4.0	37.0	4.0	39.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1	1	0.6802101881392488
1	2	2.0630425970691633
1	3	2.185954867183419
1	4	2.6434829170650502
1	5	2.672943657479017
1	6	1.284670570646945
1	7	1.2882575274596633
1	8	1.6633662865326002
1	9	0.5431824844325845
1	10	1.1463392466934934
1	11	-8.94455353931605
1	12	-11.483467047711994
1	13	-21.832487134122655
1	14	-19.82196426166316
1	15	-14.930616410049092
1	16	-21.306601592901178
1	17	-22.44698118379862
1	18	-20.868394071673265
1	19	-9.312536238339188
1	20	-12.74614688394799
1	21	-9.49639823598412
1	22	-6.823062844438208
1	23	-6.8139010467985806
1	24	-7.195448087247481
1	25	-6.312998837541155
1	26	-0.21530430105391218
1	27	-0.8843535258569233
1	28	-0.9312076439804642
1	29	-1.98169020575558
1	30	-1.5303838660470994
1	31	-2.824337242566582
1	32	-15.429683271592165
1	33	-3.8376892564090497
1	34	-4.813210706306165
1	35	-4.532931471566943
1	36	-1.629878417091149
1	37	-1.9432858612861246
1	38	-0.6545140189151262
1	39	1.6422715852759104
1	40	3.9094475414971086
1	41	-6.440466009356362
1	42	-10.590117499459755
1	43	-14.605918694879943
1	44	-15.407782173568712
1	45	-16.054030175128638
1	46	-17.777926987304483
1	47	-17.503165696247688
1	48	-15.952364403498176
1	49	-14.179658111266843
1	50	-16.018487869429123
1	51	-17.23564881541875
1	52	-17.1997737411334
1	53	-16.990365254900368
1	54	-17.184801735390906
1	55	-16.322256866317282
1	56	-15.513721032209503
2	1	0.9183054262344825
2	2	2.4797092637358276
2	3	2.3331410143695663
2	4	3.3675088910910276
2	5	2.9186146531500086
2	6	1.5768783628547425
2	7	1.3661796053817454
2	8	1.9425870657533792
2	9	1.7812777225278182
2	10	2.112789463143713
2	11	-5.506241851004361
2	12	-12.260523324768268
2	13	-16.500236051871575
2	14	-16.140146079844982
2	15	-8.648148877581562
2	16	-5.912662198961783
2	17	-4.5043404911579294
2	18	-13.320775024054218
2	19	-4.181583857386805
2	20	-5.156320044121152
2	21	1.6507879112020305
2	22	2.3825648611895005
2	23	1.8138045809070462
2	24	2.068621176821786
2	25	1.9683864438441248
2	26	1.7316653959157833
2	27	2.65568976418637
2	28	1.9335109707381477
2	29	2.042119318053942
2	30	3.1471053114420826
2	31	-1.1187095369388764
2	32	-10.800895392804286
2	33	-0.7294641481839435
2	34	-0.2049855980810591
2	35	-0.5870440256794964
2	36	5.323584786372056
2	37	5.868402450402186
2	38	6.4666981022969985
2	39	7.603310546314873
2	40	10.389967022016588
2	41	0.21429589540554161
2	42	-2.9851391444814013
2	43	-5.816957655918904
2	44	-11.55713282291936
2	45	-5.208792079890539
2	46	-13.153468112845607
2	47	-12.57675876984076
2	48	-10.474009425143198
2	49	-12.025978457587188
2	50	-10.554202155143408
2	51	-10.07655790632784
2	52	-9.67271746407712
2	53	-9.77175053628565
2	54	-15.883935934525105
2	55	-10.583079377139791
2	56	-10.285366053854524
3	1	1.9958248060794475
3	2	3.4409495738133486
3	3	3.26901134558873
3	4	4.124379927031832
3	5	4.168790833558752
3	6	3.4923117666602366
3	7	2.235101381280259
3	8	2.5387815689246267
3	9	2.696006404698366
3	10	3.627236256660275
3	11	0.7855129058666748
3	12	0.8205196632514635
3	13	-5.9512578982422575
3	14	-7.182429377942231
3	15	-3.931446974833147
3	16	1.9957239884941735
3	17	0.9790985504206482
3	18	1.22961257284501
3	19	-0.24359936126277404
3	20	0.007175375188221977
3	21	2.3773559168398037
3	22	2.3945451289837223
3	23	1.8638398169887935
3	24	1.924153241656171
3	25	1.9394928568110075
3	26	1.639346861736783
3	27	1.7310949791264676
3	28	1.8270980038600655
3	29	1.8173131165035556
3	30	1.750347030962871
3	31	1.7072242192274416
3	32	1.0877585888729548
3	33	2.085898783458063
3	34	2.540609891700477
3	35	1.109925671290199
3	36	4.867629888556689
3	37	4.809205833066038
3	38	6.182695283410457
3	39	7.017686867667933
3	40	8.806457508274516
3	41	7.8809625620722095
3	42	3.756228015490411
3	43	-1.835985140062668
3	44	0.1320849360658407
3	45	4.357099392977677
3	46	-3.57277748564335
3	47	-6.253996261031741
3	48	-4.176616895192527
3	49	-8.570023559771824
3	50	-4.344899829562014
3	51	-4.753090675883865
3	52	-4.311899986980574
3	53	-4.184717414368809
3	54	-6.933971170606853
3	55	-5.178569158676083
3	56	-2.5926246866945526
4	1	2.007194315123371
4	2	3.213042597069162
4	3	2.97960566083421
4	4	4.488721012303145
4	5	4.005483340018699
4	6	2.6576864436628185
4	7	2.2469876861898257
4	8	2.8617789849453033
4	9	2.0701666114167097
4	10	2.9915773419315883
4	11	4.1038591590966504
4	12	4.054628190383248
4	13	5.6937033420678205
4	14	3.6113690716701683
4	15	6.7114470820143985
4	16	6.404509518209931
4	17	6.218891832074391
4	18	5.168113864834673
4	19	3.97397169816875
4	20	3.3244880366869296
4	21	3.0083636687777826
4	22	2.911857790482429
4	23	1.9734005405030075
4	24	2.4807423889429927
4	25	3.3219217973794812
4	26	2.1680290322794207
4	27	2.4799321884287906
4	28	2.1981574353846156
4	29	2.3976748736095033
4	30	2.0966002609370307
4	31	2.4550278367985
4	32	3.7425389506300597
4	33	1.8321520134322213
4	34	2.5121861190906642
4	35	3.0877034490679733
4	36	4.907423170210439
4	37	4.2583014403011745
4	38	6.23437486997376
4	39	7.4295731725774985
4	40	9.391987224036793
4	41	6.8809625620722095
4	42	7.958295198952943
4	43	5.53455749559625
4	44	11.47317020738367
4	45	8.280096808998348
4	46	6.943501584124089
4	47	6.833342240260251
4	48	6.7603340092002355
4	49	3.656849825241096
4	50	7.645797844856592
4	51	4.858795629025696
4	52	5.078797687438033
4	53	3.91915855462344
4	54	0.4009125503233797
4	55	4.822981228920813
4	56	3.082310713822242
5	1	2.2748945735213084
5	2	3.6967635273017194
5	3	3.5015694851236177
5	4	4.682519461915554
5	5	3.9362326940238646
5	6	3.6085908364276804
5	7	2.490915334768637
5	8	2.585293196831607
5	9	2.579727334930922
5	10	3.1388641636370167
5	11	4.808768719820165
5	12	6.86703129115844
5	13	7.746416520362395
5	14	6.538500854615911
5	15	7.347622792608714
5	16	7.298049569889518
5	17	6.258168317862506
5	18	6.787752107728728
5	19	6.0122145922256
5	20	5.100198631002179
5	21	3.842472195909572
5	22	4.394545128983722
5	23	4.468490979779489
5	24	4.366013706772449
5	25	3.172050996345888
5	26	3.2905096524344586
5	27	3.52179265354507
5	28	2.8270980038600655
5	29	3.421964279294251
5	30	3.1224400542186856
5	31	1.7072242192274416
5	32	3.6226423098031866
5	33	2.1789220392720168
5	34	2.726656403328384
5	35	1.9936466015227552
5	36	2.21646709785902
5	37	2.251066298182316
5	38	2.926881329922086
5	39	5.645593844412126
5	40	6.08552727571638
5	41	-1.467874647230115
5	42	6.8492512713043645
5	43	10.954712534355938
5	44	9.759991912810026
5	45	7.217564509256746
5	46	9.28768763063572
5	47	8.815771180828726
5	48	9.707104035040029
5	49	10.05788341697236
5	50	9.74812342625194
5	51	10.200397696209158
5	52	9.89740233860082
5	53	9.722259329817238
5	54	11.066028829393147
5	55	11.472593632021592
5	56	11.26784042958452
6	1	2.530708527009679
6	2	3.8595542249761365
6	3	4.05970902000734
6	4	5.077868299124852
6	5	4.54088385681456
6	6	3.9109164178230245
6	7	2.7234734743035176
6	8	3.2132001735757925
6	9	2.951820358186737
6	10	3.9993292799160898
6	11	5.878536161680628
6	12	7.84377547720495
6	13	7.211532799432163
6	14	8.561756668569394
6	15	7.905762327492436
6	16	6.623630965238359
6	17	7.048865992281115
6	18	6.485426526333377
6	19	5.733144824783739
6	20	5.821128863560318
6	21	3.7727047540491014
6	22	4.441056756890696
6	23	4.1429095844306545
6	24	4.9706648695631515
6	25	3.9394928568110075
6	26	3.081207326853061
6	27	3.52179265354507
6	28	3.1061677713019265
6	29	3.7708014885965824
6	30	3.1224400542186856
6	31	3.5444335215530174
6	32	5.0412469609659745
6	33	4.109154597411546
6	34	3.9359587289097817
6	35	3.551786136406477
6	36	4.425769423440411
6	37	3.3208337400427794
6	38	5.112927841549993
6	39	5.645593844412126
6	40	2.5273877408326584
6	41	3.0670090737001168
6	42	7.756228015490411
6	43	12.6291311390071
6	44	10.643712843042586
6	45	7.845471486000932
6	46	10.450478328310137
6	47	11.211120018038027
6	48	9.84663891876096
6	49	10.150906672786313
6	50	9.980681565786824
6	51	10.432955835744039
6	52	9.874146524647337
6	53	10.350166306561423
6	54	11.159052085207101
6	55	9.263291306440195
6	56	10.546910197026378
7	1	1.3766387595678182
7	2	3.513042597069166
7	3	2.907383438611987
7	4	4.372054345636478
7	5	3.9443722289075893
7	6	3.2632419992183728
7	7	2.60254324174538
7	8	3.1062234293897433
7	9	2.914611055861151
7	10	3.399910675264927
7	11	5.448303603541092
7	12	7.112961523716578
7	13	7.060370008734488
7	14	8.02803573833684
7	15	7.43366930423662
7	16	7.018398407098825
7	17	6.899447387629948
7	18	6.995891642612449
7	19	4.276749475946527
7	20	5.921710258909151
7	21	3.1500303354444554
7	22	3.659080012704649
7	23	2.178956096058563
7	24	4.122409055609658
7	25	4.144144019601704
7	26	2.159695698946088
7	27	3.2299321884287906
7	28	3.05093521316239
7	29	3.1754526513872747
7	30	2.9160447053814735
7	31	2.832805614576273
7	32	5.695316728407835
7	33	4.026596457876664
7	34	2.576075007979547
7	35	2.984925671290199
7	36	0.23797872576599488
7	37	1.3388569958567338
7	38	1.3704859810848724
7	39	4.110128728133052
7	40	-1.1191238870743199
7	41	-8.19403743792779
7	42	4.202739643397386
7	43	8.951224162262914
7	44	6.256503540717002
7	45	3.949541253442792
7	46	7.093501584124091
7	47	6.611120018038026
7	48	7.629778453644683
7	49	8.634627603018874
7	50	6.4457978448565925
7	51	8.764351184581251
7	52	8.853797687438032
7	53	8.577491887956775
7	54	10.27591255032338
7	55	9.881314562254147
7	56	8.118421824933353
8	1	1.5687119302995285
8	2	2.764262109264287
8	3	3.123237097148575
8	4	4.236688491977944
8	5	3.9333966191514875
8	6	2.4815346821452025
8	7	2.2952261685746436
8	8	2.306223429389746
8	9	2.5048549583001787
8	10	3.056617992338097
8	11	4.484888969394753
8	12	6.880644450545844
8	13	7.853052935563753
8	14	7.580474762727086
8	15	7.6800107676512575
8	16	7.351934992464674
8	17	6.8134717778738505
8	18	7.053208715783185
8	19	5.298090939361163
8	20	6.244271234518905
8	21	3.245762042761527
8	22	4.460909280997335
8	23	3.524078047278074
8	24	4.435213933658439
8	25	3.763656214723653
8	26	2.5712810647997486
8	27	2.8604199933068415
8	28	2.468618139991655
8	29	2.3266721635824013
8	30	2.1264105590400106
8	31	3.9596348828689614
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115	9	0.5431824844325845
115	10	1.1344344847887342
115	11	-1.7659821107446234
115	12	0.7784377141927692
115	13	-0.9158204674559869
115	14	-6.702916642615541
115	15	-2.6568068862395684
115	16	-9.163744450044035
115	17	-12.339838326655766
115	18	-5.035060738339929
115	19	-4.89586957167252
115	20	-5.329480217281322
115	21	-2.0202077597936423
115	22	-1.6563961777715406
115	23	-2.1948534277509637
115	24	-0.10020999200938974
115	25	-1.7891893137316295
115	26	-0.15578049153010198
115	27	0.9846940931906971
115	28	-0.3002552630280846
115	29	-0.29121401527938673
115	30	0.16009232442909394
115	31	-0.11005152828087006
115	32	-6.2511118430207375
115	33	2.0194536007338115
115	34	0.7344083413128857
115	35	0.5384970998616296
115	36	1.786788249575519
115	37	2.1281427101424484
115	38	3.4883431239420197
115	39	5.451795394799717
115	40	7.242780874830444
115	41	8.119057800167447
115	42	2.3741682148259606
115	43	4.810747971786725
115	44	2.4731702073836708
115	45	2.457874586776125
115	46	-3.504117463494957
115	47	-6.122213315295307
115	48	-7.1190310701648425
115	49	-8.155848587457317
115	50	-2.078011678952933
115	51	1.8714940417241088
115	52	2.288321496961842
115	53	1.938206173671059
115	54	1.6961506455614739
115	55	2.9277431336827213
115	56	5.164850396361928
116	1	1.3385952813069508
116	2	2.247825205764812
116	3	2.5399921342641605
116	4	2.169880432593004
116	5	2.3204591854293284
116	6	1.9088941731314222
116	7	1.2199345460932065
116	8	1.132310385911488
116	9	0.6363501862959353
116	10	1.5857802404823147
116	11	-1.6169137877632558
116	12	-3.9772558675877683
116	13	-2.7439778173524694
116	14	-10.974138174706638
116	15	-2.4228524348938123
116	16	-6.567471158118568
116	17	-3.8864221775874412
116	18	-2.0454127052136357
116	19	-1.7612940023143437
116	20	-2.5054636541343243
116	21	2.2924216397922805
116	22	3.4471234909655166
116	23	3.1985213134498665
116	24	1.6865394903922706
116	25	2.9789266282973514
116	26	1.6433913511200018
116	27	1.906019144950534
116	28	0.958543908814562
116	29	0.5928439557351055
116	30	0.36713166190321544
116	31	-0.8367596028150288
116	32	-10.940552836809555
116	33	-0.8190557160363809
116	34	-0.07718586158566865
116	35	-1.1074656330576254
116	36	2.567326551852947
116	37	3.239943952378475
116	38	2.2150511984761785
116	39	5.350346119437404
116	40	8.350441330316986
116	41	6.228788649028733
116	42	2.23752225209304
116	43	-9.563993229041433
116	44	2.9804165841952646
116	45	2.168019514312359
116	46	-4.673889720223734
116	47	-7.8758365037011036
116	48	-8.638699807224883
116	49	-3.7045028317637367
116	50	-6.2498543290564506
116	51	-0.7682575110709244
116	52	-2.216854486475011
116	53	-2.2170733294345304
116	54	-5.312130927937492
116	55	-3.165424568180633
116	56	-0.49353469680577433
117	1	0.020869528798584724
117	2	1.736119520146083
117	3	1.2958449770735285
117	4	2.1297466533287874
117	5	1.6482183827537398
117	6	1.905549691526069
117	7	0.002543241745378566
117	8	0.2293003524666659
117	9	0.19153413278423415
117	10	0.09414144449569051
117	11	-15.667081011843525
117	12	-19.104346168591114
117	13	-13.285783837419359
117	14	-15.245041184740085
117	15	-9.466330695763379
117	16	-13.383524669824254
117	17	-9.62555261237005
117	18	-5.346416049695243
117	19	-7.309788985591933
117	20	-5.237905125706231
117	21	-1.8480465876324672
117	22	-1.4659199872953508
117	23	-0.3633515962491316
117	24	-4.591052482851879
117	25	-0.07124059578291053
117	26	1.573157237407628
117	27	1.8491629576595585
117	28	-1.2086801714529969
117	29	0.6831449590795842
117	30	-0.9204937561569864
117	31	-3.197963616192954
117	32	-18.29506788697678
117	33	-3.431095849815641
117	34	-1.9066172997127566
117	35	-2.7362281748636477
117	36	-1.0447135819263131
117	37	-2.4322968502971136
117	38	-2.8083601727612795
117	39	2.0158979589022827
117	40	-1.4422008101512418
117	41	-0.3498066686970205
117	42	-3.9664911258333824
117	43	-10.306468145429394
117	44	-4.731957997744535
117	45	-3.2600741311725905
117	46	-5.148806108183603
117	47	-10.327341520423511
117	48	-7.4413753925091655
117	49	-5.196141627750357
117	50	-6.5542021551434075
117	51	-3.0625718923418255
117	52	-3.290433081792738
117	53	-2.6552004197355323
117	54	-6.168318218907391
117	55	-3.028300822361235
117	56	1.3626525941641248
118	1	0.5674282332520306
118	2	1.8656741760165332
118	3	1.5994887017698858
118	4	2.822054345636481
118	5	2.231214334170744
118	6	0.5816630518499579
118	7	0.4235958733243237
118	8	-0.641144991662884
118	9	0.5356636874400991
118	10	0.16701593842282136
118	11	-11.367485870143117
118	12	-10.306775318388684
118	13	-12.597524728107619
118	14	-1.8482800511368467
118	15	-18.04527806418443
118	16	-8.201338435006441
118	17	-10.783447349212157
118	18	-4.443582041598077
118	19	-3.6903557872113666
118	20	-4.221710793722426
118	21	1.1276619143918225
118	22	0.639343170599389
118	23	0.8552718855322468
118	24	-0.9473277864955989
118	25	0.6494071774964425
118	26	1.0873272778934577
118	27	1.2378269252708947
118	28	0.5443562657939722
118	29	2.2701894934925377
118	30	1.4357815474867337
118	31	1.1907003514183785
118	32	-11.7849464294869
118	33	-2.2812982789654406
118	34	0.5589697448216526
118	35	-1.1006006444992735
118	36	1.6313997783975758
118	37	2.3085938379619932
118	38	3.187591244242771
118	39	5.364076096554108
118	40	6.90192874450463
118	41	9.091488877861682
118	42	0.381687011818439
118	43	4.264382056999757
118	44	3.911766698611739
118	45	0.38769914817963524
118	46	-6.193340521139067
118	47	-8.999406297751447
118	48	-5.595221546355319
118	49	-5.860109239086391
118	50	-4.291044260406565
118	51	-4.090911973313485
118	52	-3.836991786246177
118	53	-3.0843502173063833
118	54	-10.241192712834517
118	55	-7.69631701669322
118	56	-5.301315017171909
119	1	-0.5608612404321818
119	2	0.9380425970691633
119	3	0.06988343861198842
119	4	1.6970543456364808
119	5	0.5568722289075865
119	6	-0.4117580007816244
119	7	0.002543241745378566
119	8	0.3062234293897461
119	9	-2.072888944138846
119	10	-1.987589324735076
119	11	-13.426696396458908
119	12	-10.84953847628342
119	13	-3.7521299912655124
119	14	-4.571964261663162
119	15	-7.028830695763379
119	16	-9.244101592901178
119	17	-13.75055261237005
119	18	-4.65410835738755
119	19	-5.973250524053473
119	20	-3.9157897410908475
119	21	-4.362469664555547
119	22	-2.653419987295351
119	23	-4.296043903941438
119	24	-6.052590944390339
119	25	-4.643355980398297
119	26	-3.215304301053912
119	27	-3.420067811571208
119	28	-3.51156478683761
119	29	-2.6870473486127224
119	30	-2.2714552946185265
119	31	-3.779694385423724
119	32	-11.429683271592165
119	33	-3.0609035421233344
119	34	-4.16142499202045
119	35	-3.390074328709801
119	36	1.0754787257659935
119	37	1.163856995856733
119	38	1.8454859810848738
119	39	4.035128728133053
119	40	5.7308761129256816
119	41	4.8809625620722095
119	42	-0.5722603566026123
119	43	-0.061275837737085226
119	44	-1.130996459282997
119	45	-0.5629587465572072
119	46	-8.70649841587591
119	47	-12.601379981961973
119	48	-10.032721546355319
119	49	-7.465372396981127
119	50	-8.679202155143408
119	51	-9.485648815418749
119	52	-9.708702312561968
119	53	-12.785008112043226
119	54	-11.024087449676621
119	55	-12.206185437745852
119	56	-9.594078175066645
120	1	0.3104622889795863
120	2	2.0483367147162213
120	3	1.9926775562590464
120	4	2.233819051518836
120	5	2.2590781112605285
120	6	1.7426537639242596
120	7	0.6496020652747916
120	8	0.8356351940956301
120	9	0.526375761743509
120	10	1.736675381147279
120	11	-9.698755219988321
120	12	-3.000273770401069
120	13	-7.851394697147864
120	14	-6.204317202839633
120	15	-5.583977754586908
120	16	-10.60071923996
120	17	-3.1549643770759346
120	18	-2.1835201220934337
120	19	-1.5541328769946503
120	20	-2.3827015057967316
120	21	0.4867950413268076
120	22	2.0634917774105332
120	23	0.5642502137056198
120	24	-3.7584732973315162
120	25	-0.3970324509865293
120	26	1.4464604048284428
120	27	0.903461600193495
120	28	1.234758742574158
120	29	1.0225114749166906
120	30	1.1881035289108866
120	31	-3.9083708560119597
120	32	-8.15027150688628
120	33	-1.1234035421233344
120	34	-11.775395580255744
120	35	-3.9488978581215655
120	36	-1.442903627175184
120	37	-1.821437121790325
120	38	-4.007455195385713
120	39	1.4910110810742303
120	40	-5.5191238870743184
120	41	-3.177860967339555
120	42	-5.667848591896728
120	43	-4.2928934847959095
120	44	-3.958202341635939
120	45	-15.346046981851325
120	46	-14.927086651170027
120	47	-13.847703511373737
120	48	-14.124633311061201
120	49	-14.61243122051054
120	50	-13.495378625731643
120	51	-14.456237050712867
120	52	-13.874143489032557
120	53	-13.935743406160872
120	54	-14.104969802617799
120	55	-13.467214849510558
120	56	-11.994813469184292
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	295.0
5	10.0
6	7.0
7	19.0
8	59.0
9	93.0
10	31.0
11	39.0
12	49.0
13	55.0
14	76.0
15	112.0
16	186.0
17	224.0
18	258.0
19	314.0
20	424.0
21	481.0
22	596.0
23	694.0
24	754.0
25	827.0
26	873.0
27	867.0
28	925.0
29	973.0
30	1119.0
31	1390.0
32	1651.0
33	1944.0
34	2226.0
35	2590.0
36	2827.0
37	2401.0
38	1237.0
39	387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.0	0.0	0.0	100.0
2	0.0	100.0	0.0	0.0
3	0.0	0.0	0.0	100.0
4	0.0	100.0	0.0	0.0
5	0.0	100.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	99.30774071743235	0.6515381482989672	0.01480768518861289	0.025913449080072558
8	99.51875023137008	0.3924036574982416	0.06293266205160479	0.025913449080072558
9	97.54887440758294	1.4292061611374407	0.5850118483412322	0.4369075829383886
10	54.818050568244914	30.52604301632547	5.504756968866841	9.151149446562766
11	36.05804827484081	26.980601214275136	18.59173700577521	18.369613505108838
12	35.33483878132751	13.71561840595269	22.326287343131085	28.623255469588717
13	25.55066079295154	13.193647503054084	25.191574427127676	36.06411727686669
14	21.441001147765558	15.683661001888261	28.386834018290198	34.48850383205598
15	25.28279494121574	17.156844564773948	26.721803953566	30.838556540444312
16	30.006665679158644	17.41964153458747	26.729373426159086	25.844319360094804
17	28.555559669715258	22.19424593623875	23.379123930832748	25.871070463213243
18	30.749296608914555	23.996742188656896	20.6426773285947	24.61128387383385
19	26.463509460510238	28.30007035213093	19.883733846780462	25.352686340578366
20	29.28653411825688	23.495871746454885	21.05594431485801	26.161649820430227
21	28.034649983341353	22.8630659312183	23.399844519305518	25.70243956613482
22	28.03835190463851	21.741383778180875	25.30633398733943	24.91393032984119
23	30.955465886795245	23.57013289897457	23.229556139636472	22.244845074593712
24	28.056861511124275	22.385518083885536	22.555806463554585	27.001813941435604
25	27.4608521822826	24.432680561211267	21.604412690186205	26.50205456631992
26	24.869313758202647	23.312201089978867	25.199273347421496	26.61921180439699
27	24.186322064649907	19.780057022253487	25.337875365645946	30.69574554745066
28	24.576428280131985	24.94346198049902	25.41059578096615	25.069513958402844
29	26.407968304513645	22.571925797015588	27.38549265005369	23.63461324841708
30	23.83632660618404	22.025550823921495	30.08331790409183	24.054804665802628
31	26.317546267106778	21.229091718280607	29.666580128324	22.78678188628862
32	29.55832808855651	18.592425308207766	28.928954870238048	22.920291732997665
33	27.866360130525063	20.227677247107685	31.759863541975676	20.146099080391576
34	24.37208268504112	21.14543972734682	35.2115284878121	19.270949099799957
35	23.585185185185185	24.933333333333334	32.781481481481485	18.7
36	23.182121414276182	26.947594692758138	29.67533911496553	20.19494477800015
37	24.942520210635617	24.846102499443745	30.226952458651635	19.984424831269003
38	24.936981020166073	27.491103202846972	30.608689205219452	16.9632265717675
39	23.939686548317614	20.485775830052738	26.349996286117506	29.224541335512143
40	15.002792776019364	15.479426549990691	21.984732824427482	47.533047849562465
41	17.418728772440563	17.332885455156198	23.849512932482362	41.39887283992088
42	19.82419701802537	24.805281507306578	30.669089830131295	24.701431644536758
43	13.39922711058264	13.272889417360284	25.24152794292509	48.086355529131986
44	14.385651559127782	15.159633846840812	23.636228324774873	46.81848626925653
45	22.824636821820338	22.635635932404387	32.541506077675656	21.998221168099615
46	22.37037037037037	22.874074074074073	32.57037037037037	22.185185185185187
47	19.337037037037035	19.48148148148148	32.07407407407407	29.10740740740741
48	21.149501907195496	22.54934636892197	35.01092471206903	21.290227011813503
49	15.50514239037612	14.72171685293135	24.02628745405265	45.746853302639884
50	19.224064389303066	22.350803011757726	36.59730722154223	21.82782537739698
51	22.30760663771021	22.18138619742362	34.05353231614508	21.457474848721088
52	20.119618113600062	23.544708198670083	32.68323488985475	23.652438797875107
53	19.600934614100805	24.12194488743834	35.192671438638136	21.08444905982272
54	18.910303928836175	20.148257968865828	32.083024462564865	28.858413639733136
55	20.91660795728429	20.946271645222293	34.354258593199596	23.78286180429382
56	21.153917460788314	23.87926879009233	31.973747636174863	22.99306611294449
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	2.0
11	4.0
12	14.5
13	30.5
14	36.0
15	49.0
16	62.0
17	73.0
18	84.0
19	81.0
20	78.0
21	106.0
22	162.5
23	191.0
24	217.5
25	244.0
26	315.5
27	387.0
28	465.5
29	615.0
30	686.0
31	757.5
32	829.0
33	812.0
34	795.0
35	756.5
36	718.0
37	667.0
38	569.0
39	522.0
40	505.0
41	488.0
42	485.0
43	482.0
44	497.0
45	512.0
46	542.5
47	606.5
48	640.0
49	679.5
50	719.0
51	776.5
52	834.0
53	899.0
54	1060.5
55	1157.0
56	1299.0
57	1441.0
58	1543.5
59	1646.0
60	1813.5
61	1981.0
62	2103.5
63	2063.0
64	1900.0
65	1729.0
66	1558.0
67	1387.5
68	1217.0
69	1087.5
70	958.0
71	813.0
72	536.0
73	404.0
74	343.0
75	282.0
76	254.0
77	226.0
78	161.5
79	76.0
80	55.0
81	38.5
82	22.0
83	13.5
84	5.0
85	3.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.018509606485766113
10	0.0
11	0.0037019212971532225
12	0.0
13	0.0
14	0.01480768518861289
15	0.1850960648576611
16	0.033317291674379
17	0.022211527782919335
18	0.0037019212971532225
19	0.022211527782919335
20	0.01480768518861289
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.1480768518861289
27	0.022211527782919335
28	0.1480768518861289
29	0.022211527782919335
30	0.02961537037722578
31	0.1850960648576611
32	0.007403842594306445
33	0.166586458371895
34	0.07033650464591122
35	0.04812497686299189
36	0.1147595602117499
37	0.17399030096620147
38	0.13697108799466923
39	0.3220671528523304
40	0.5849035649502091
41	0.8144226853737089
42	0.18879798615481436
43	0.37389405101247547
44	0.5145670603042979
45	0.10735571761744346
46	0.04812497686299189
47	0.04812497686299189
48	0.037019212971532225
49	0.2961537037722578
50	0.19249990745196757
51	0.2813460185836449
52	0.34798060193240293
53	0.1850960648576611
54	0.12216340280605635
55	0.1628845370747418
56	0.1628845370747418
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
56	27013.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	88.58697664087661
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.6105307145842	82.92673897752934
2	4.262432093606352	7.551919446192574
3	1.0112829084830754	2.6875948617332397
4	0.4011700793982449	1.4215377781068375
5	0.2381947346427079	1.0550475696886683
6	0.12536564981195153	0.66634583348758
7	0.08775595486836607	0.5441824306815237
8	0.0710405348934392	0.5034612964128383
9	0.041788549937317176	0.33317291674379
>10	0.14625992478061012	2.121200903268796
>50	0.004178854993731718	0.18879798615481436
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCCCCCGACTGCTCTTCC	51	0.18879798615481436	No Hit
CACAAGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTCC	43	0.15918261577758858	No Hit
CACAAGGGGGCCGAATAGCCTCTCCACCCAAGCGGCCGGCCAACCTGCGTGCAATC	38	0.14067300929182244	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.01480768518861289	0.0	0.0	0.0	0.0
11	0.018509606485766113	0.0	0.0	0.0	0.0
12	0.018509606485766113	0.0	0.0	0.0	0.0
13	0.022211527782919335	0.0	0.0	0.0	0.0
14	0.02961537037722578	0.0	0.0	0.0	0.0
15	0.033317291674379	0.0	0.0	0.0	0.0
16	0.033317291674379	0.0	0.0	0.0	0.0
17	0.033317291674379	0.0	0.0	0.0	0.0
18	0.033317291674379	0.0	0.0	0.0	0.0
19	0.037019212971532225	0.0	0.0	0.0	0.0
20	0.04072113426868545	0.0	0.0	0.0	0.0
21	0.04072113426868545	0.0	0.0	0.0	0.0
22	0.04442305556583867	0.0	0.0	0.0	0.0
23	0.04442305556583867	0.0	0.0	0.0	0.0
24	0.04442305556583867	0.0	0.0	0.0	0.0
25	0.04442305556583867	0.0	0.0	0.0	0.0
26	0.051826898160145116	0.0	0.0	0.0	0.0
27	0.05552881945729834	0.0	0.0	0.0	0.0
28	0.05923074075445156	0.0	0.0	0.0	0.0
29	0.05923074075445156	0.0	0.0	0.0	0.0
30	0.05923074075445156	0.0	0.0	0.0	0.0
31	0.06293266205160478	0.0	0.0	0.0	0.0
32	0.066634583348758	0.0	0.0	0.0	0.0
33	0.066634583348758	0.0	0.0	0.0	0.0
34	0.066634583348758	0.0	0.0	0.0	0.0
35	0.066634583348758	0.0	0.0	0.0	0.0
36	0.066634583348758	0.0	0.0	0.0	0.0
37	0.066634583348758	0.0	0.0	0.0	0.0
38	0.066634583348758	0.0	0.0	0.0	0.0
39	0.066634583348758	0.0	0.0	0.0	0.0
40	0.066634583348758	0.0	0.0	0.0	0.0
41	0.07033650464591122	0.0	0.0	0.0	0.0
42	0.07033650464591122	0.0	0.0	0.0	0.0
43	0.07033650464591122	0.0	0.0	0.0	0.0
44	0.07403842594306445	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGAGC	15	0.008032168	50.4803	41
GCCCAGT	20	3.9207964E-4	50.4803	41
GAGCACT	20	3.9207964E-4	50.4803	38
TACGCCC	15	0.008032168	50.4803	38
GCCATGA	15	0.008032168	50.4803	39
CTGTCGA	20	3.9207964E-4	50.4803	40
GTACGCC	15	0.008092068	50.38577	37
GCAAAGT	15	0.0082128635	50.19776	49
TGCTTTT	15	0.0082128635	50.19776	47
CGAAAGC	15	0.0082128635	50.19776	46
AAAGCCA	15	0.0082128635	50.19776	48
AAGCCAT	15	0.0082128635	50.19776	49
GAAAGCC	15	0.0082128635	50.19776	47
TTGCTTT	15	0.0082128635	50.19776	46
ATGAGCA	15	0.0082128635	50.19776	42
CCCAGTT	20	4.0313168E-4	50.197758	42
CCAACAC	15	0.008273762	50.104282	43
CCAGTTC	20	4.068702E-4	50.104282	43
AGACGAA	15	0.008273762	50.104282	43
ACGAAAG	15	0.008273762	50.104282	45
>>END_MODULE