FastQCFastQC Report
Fri 17 Jun 2016
SRR1526360_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1526360_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64424
Sequences flagged as poor quality0
Sequence length56
%GC55

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCCCTTGCCTAGCAAGCGC2400.3725319756612443No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAACGATTAAG2140.33217434496460946No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGC2020.31354774618154724No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1750.27163789891965723No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCTGTCTCAAAGATTAAG1310.20334037004842917No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTCGTCTCAAAGATTAAG1230.1909226375263877No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCCCGTCTCAAAGATTAAG890.1381472743077114No Hit
AGGACTGGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTCCCTACCAAGCGC870.13504284117720106No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCA830.1288339749161803No Hit
AGGACTGGGGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCCTGCCTAGCAAGCGCA800.12417732522041476No Hit
AGGACTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGCCTCAACGATTAAG670.10399850987209734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTCA251.9367666E-550.5449541
ATACAAA203.9682724E-450.4653536
TATGCCT402.4429028E-950.38600535
TCGCCTA204.077541E-450.18872542
CTCAGGT150.00827073750.18872542
CCATCTA150.00829639550.14945233
CCCTCAC150.00832211150.1102443
ATTTTAT150.00832211150.1102444
CCTCAAC204.1091954E-450.1102443
CCGACGC150.00832211150.1102443
CGACTCC355.095535E-850.1102444
CGTCACA150.00832211150.1102444
CGATTCA204.1250957E-450.071149
CGATTAA402.582965E-950.071149
CAGAATG150.00834788850.07109549
ACGATTA402.5993359E-950.0320148
AACGATT550.050.0320147
AGGGTGT150.00837372350.03200550
ACCAAGC600.050.03200548
CGGTTGA204.1730903E-449.95401414